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Plasmid#230837PurposeEf1a-MS2-AIDDead-T2A-GFP-wPREDepositorInsertMS2-AIDDead
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGH518
Plasmid#230836PurposeEf1a-MS2-AID*-T2A-GFP-wPREDepositorInsertMS2-AID*
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
yWL234 MCP-GFP-PABPC1*
Plasmid#197054PurposepUBC-NLS-HA-2xMCP-1xGFP-PABPC1*DepositorInsertpubc-nls-ha-MCP-GFP-PABPC1*
UseLentiviralTagsExpressionMammalianMutationPromoterubcAvailable sinceMarch 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
MCP-APEX2
Plasmid#154936PurposeNLS-V5-MCP-APEX2DepositorInsertNLS-V5-MCP-APEX2
UseLentiviralTagsV5ExpressionMammalianMutationPromoterCMVAvailable sinceSept. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJK2014
Plasmid#171931PurposeTo express sygl-1 fused to 24 copies of MS2 loops.DepositorInsertPsygl-1::24XMS2 loops::3X Flag::sygl-1 ORF::sygl-1 3'UTR
UseTagsN-terminal Tag 24X MS2 loopsExpressionWormMutationPromoter-Available sinceDec. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
yWL229 MCP-GFP-eRF3
Plasmid#197053PurposepUBC-NLS-HA-2xMCP-1xGFP-eRF3DepositorInsertpubc-nls-ha-MCP-GFP-eRF3
UseLentiviralTagsExpressionMammalianMutationPromoterubcAvailable sinceMarch 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
yWL268 MCP-GFP
Plasmid#197052PurposepUBC-NLS-HA-2xMCP-2xGFPDepositorInsertpubc-nls-ha-MCP-2xGFP
UseLentiviralTagsExpressionMammalianMutationPromoterubcAvailable sinceMarch 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMH183
Plasmid#122856PurposeMS2-modified guide RNA targeting the FT promoter with GreenGate D-E flanking sequencesDepositorInsertMS2-modified sgRNA targeting the Arabidopsis FT promoter
UseGolden gate compatible cloning vectorTagsExpressionMutationPromoterAvailable sinceJune 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMH184
Plasmid#122857PurposeMS2-modified guide RNA targeting the FT promoter with GreenGate E-F flanking sequencesDepositorInsertMS2-modified sgRNA targeting the Arabidopsis FT promoter
UseGolden gate compatible cloning vectorTagsExpressionMutationPromoterAvailable sinceMarch 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
Lenti-SAM-Puro
Plasmid#198713PurposeFor expression of guide RNAs and the SAM transcriptional activator for CRISPRa in mammalian cells.DepositorInsertsU6-MS2-gRNAscaffold
EF1a-MPH-Puro
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF1a and U6Available sinceJan. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
Lenti-SAM-Cre
Plasmid#198714PurposeFor expression of guide RNAs, the SAM transcriptional activator for CRISPRa and Cre in mammalian cellsDepositorInsertsU6-MS2-gRNAscaffold
Pgk-MPH-Cre
UseCre/Lox and LentiviralTagsExpressionMammalianMutationPromoterPgk and U6Available sinceJan. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
dCas13d-dsRBD
Plasmid#154938PurposeBPNLS-dRfxCas13d-dsRBD-BPNLS-HADepositorInsertBPNLS-dRfxCas13d-dsRBD-BPNLS-HA
UseLentiviralTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceSept. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTK333
Plasmid#59574PurposeSIN SP6 P1234 (nsP2 P726S) SGP(14) Kozak MS2-CNOT7 (SacI mutated)-P2A-EBFP2 4xFF5DepositorInsertSGP(14) Kozak MS2-CNOT7 (SacI mutated)-P2A-EBFP2 4xFF5
UseTagsExpressionMutationPromoterAvailable sinceAug. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCML 221
Plasmid#98587PurposepMS2-CYFP. Expresses MS2 protein-CYFP fusion in E. coli for TriFC assay (kanR).DepositorInsertMS2
UseTagsExpressionMutationPromoterpLacOAvailable sinceSept. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJK2020
Plasmid#171932Purposeexpress MS2 coat protein (MCP) fused to superfolder GFP and histone subunit H2B fused to mCherry in an operonDepositorInsertPmex-5::MS2 Coat Protein::linker::sfGFP::tbb-2 3'UTR::gpd-2 intergenic sequence::H2B::mCherry::unc-54 3'UTR
UseTagssuperfolder GFP, mCherryExpressionWormMutationPromoter-Available sinceJuly 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET296-YcpLac111 CYC1p-MCP-NLS-2xyeGFP
Plasmid#104394PurposeYeast MCP-GFP expressionDepositorInsertMCP-NLS-2xyeGFP
UseTagsExpressionBacterial and YeastMutationMCP fused to NLS(SV40) and 2x yeast codon optimiz…PromoterCYC1Available sinceJan. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTK332
Plasmid#59573PurposeSIN SP6 P1234 (nsP2 P726S) SGP(14) 2xK-turn Kozak MS2-CNOT7 (SacI mutated)-P2A-EBFP2-4xFF5DepositorInsertSGP(14) 2xK-turn Kozak MS2-CNOT7 (SacI mutated)-P2A-EBFP2-4xFF5
UseTagsExpressionMutationPromoterAvailable sinceAug. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
dCas13d-dsRBD-APEX2
Plasmid#154939PurposeTetON-APEX2-V5-BPNLS-dRfxCas13d-dsRBD-BPNLS-P2A-GFPDepositorInsertTetON-APEX2-V5-BPNLS-dRfxCas13d-dsRBD-BPNLS-P2A-GFP
UseTagsV5ExpressionMammalianMutationPromoterTRE3GAvailable sinceSept. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
NLS-HA-tdMCP-tdTomato
Plasmid#183938Purposetandem MS2 binding protein labeled with tdTomato, containing a NLS and a HA-tagDepositorInserttdMCP-tdTomato (cp Synthetic)
UseTagsNLS-HAExpressionMammalianMutationnonePromoterCMV (TATA box removed)Available sinceJune 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJZC545
Plasmid#62313PurposesgRNA with 1x MS2 for yeast cellsDepositorInsertsgRNA + 1x MS2 binding module
UseTagsExpressionYeastMutationPromoterSNR52Available sinceMay 8, 2015AvailabilityAcademic Institutions and Nonprofits only