We narrowed to 24,459 results for: Spr

1. lentiCRISPR - DDX3Y sgRNA 1

   Plasmid

   #70656

   Purpose

   Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and a DDX3Y targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.

   Depositor

   David Sabatini

   Article

   Wang et al Science. 2015 Oct 15. pii: aac7041.

   Insert

   sgRNA against DDX3Y
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Oct. 29, 2015

   Availability

   Academic Institutions and Nonprofits only

2. pNIC-ZB-SPRTN-E112A-FL

   Plasmid

   #110218

   Purpose

   Expression in E. coli of full-length SPRTN protein carrying E112A mutation, a catalytically-dead mutant, with His and ZB tags at N-terminus.

   Depositor

   Kristijan Ramadan

   Article

   Vaz et al Mol Cell. 2016 Nov 17;64(4):704-719. doi

   Insert

   SPRTN (SPRTN Human)
   

   Tags

   His6 and ZB

   Expression

   Bacterial

   Mutation

   E112A, P296L (see depositor comments below)

   Available Since

   May 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. lenticrispr-wt-ltr-puro

   Plasmid

   #173428

   Purpose

   Expresses Cas9 and guide RNA, contains intact LTR

   Depositor

   Jakob Reiser

   Article

   Iaffaldano et al Front Genome Ed. 2023 Jul 13;5:1218328.

   Insert

   S. pyogenes sgRNA cassette
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   July 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLenti-Crispr-V2-HMID.v1

   Plasmid

   #103062

   Purpose

   LentiCrisprV2 with guide targeting V1-V5 GESTALT barcodes

   Depositor

   Jay Shendure

   Article

   McKenna et al Science. 2016 May 26. pii: aaf7907.

   Insert

   Cas9 plus expressed GESTALT V1-V5 guide
   

   Use

   Lentiviral

   Available Since

   Feb. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGEMHE-mEGFP-mTrim21ΔPRYSPRY

   Plasmid

   #105521

   Purpose

   T7 promotor drives in vitro transcription of mEGFP-tagged mouse TRIM21 with deleted PRYSPRY domain mRNA

   Depositor

   Melina Schuh

   Article

   Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

   Insert

   TRIM21 (Trim21 Mouse)
   

   Use

   In vitro transcription

   Tags

   mEGFP

   Mutation

   deletion of PRYSPRY domain (aa1-281)

   Promoter

   T7

   Available Since

   March 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLenti CRISPR sgSHMT1_1

   Plasmid

   #106311

   Purpose

   Express Cas9 and sgRNA targeting SHMT1

   Depositor

   Richard Possemato

   Article

   Minton et al Mol Cell. 2018 Feb 15;69(4):610-621.e5.

   Insert

   sgRNA targeting SHMT1
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   April 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. BRD4 CRISPRi plasmid

   Plasmid

   #154890

   Purpose

   Human BRD4 CRISPRi gRNA

   Depositor

   Rob Mitra, Joseph Dougherty

   Article

   Moudgil et al Cell. 2020 Aug 20;182(4):992-1008.e21. d

   Insert

   BRD4-targeting gRNA
   

   Use

   CRISPR and Lentiviral

   Available Since

   July 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. SpRY dCas9 VPR

   Plasmid

   #188511

   Purpose

   Expresses FLAG tagged SpRY dCas9 VPR

   Depositor

   Isaac Hilton

   Article

   Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

   Insert

   dCas9
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Mutation

   D10A/A61R/H840A/L1111R/D1135L/S1136W/G1218K/E1219…

   Promoter

   EF1a

   Available Since

   Sept. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. LentiCRISPR v2-sgACSL4 clone3

   Plasmid

   #162130

   Purpose

   Lentiviral sgRNA plasmid targeting human ACSL4

   Depositor

   Boyi Gan

   Article

   Lee et al Nat Cell Biol. 2020 Feb;22(2):225-234. d

   Insert

   sgACSL4 (ACSL4 Human)
   

   Use

   Lentiviral

   Available Since

   Feb. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLenti CRISPR V2 sgMTFMT_1

    Plasmid

    #106318

    Purpose

    Express Cas9 and sgRNA targeting MTFMT

    Depositor

    Richard Possemato

    Article

    Minton et al Mol Cell. 2018 Feb 15;69(4):610-621.e5.

    Insert

    sgRNA targeting MTFMT
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    April 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pNOC-CRISPR-35S

    Plasmid

    #99861

    Purpose

    Expresses Cas9-Nlux and sgRNA. Contains CEN/ARS and 35 terminator. No selection marker.

    Depositor

    Eva Farre

    Article

    Poliner et al ACS Synth Biol. 2018 Apr 20;7(4):962-968

    Type

    Empty backbone

    Use

    Algae, nannochloropsis expression

    Tags

    Cas9-Nlux

    Promoter

    Ribi promoter

    Available Since

    Feb. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. lentiCRISPRv2-7SK-Blast

    Plasmid

    #183620

    Purpose

    lentiCRISPRv2 derivative with modified 7SK promoter driving sgRNA and encoding Blasticidin resistance. Compatible with inserts designed for lentiCRISPRv2.

    Depositor

    Bill Sugden

    Article

    Hutcheson et al ACS Synth Biol. 2022 Jun 29. doi: 10.102

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    Modified 7SK

    Available Since

    July 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. CRISPR-Sa-TdTom. Reporter

    Plasmid

    #99652

    Purpose

    Red fluorescent reporter that can be activated by dSa Cas9 activator indicating activity

    Depositor

    George Church

    Insert

    RFP
    

    Expression

    Mammalian

    Mutation

    dead Cas9

    Available Since

    Dec. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLenti CRISPR sgSHMT1_2

    Plasmid

    #106312

    Purpose

    Express Cas9 and sgRNA targeting SHMT1

    Depositor

    Richard Possemato

    Article

    Minton et al Mol Cell. 2018 Feb 15;69(4):610-621.e5.

    Insert

    sgRNA targeting SHMT1
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    April 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. R777-E331 Hs.SPRY3

    Plasmid

    #70615

    Purpose

    Gateway ORF clone of human SPRY3 [NM_005840.2] with stop codon (for native or N-terminal fusions)

    Depositor

    Dominic Esposito

    Insert

    SPRY3 (SPRY3 Human)
    

    Use

    Gateway entry clone

    Available Since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. 474 p5E-basprom

    Plasmid

    #31158

    Depositor

    Nathan Lawson

    Insert

    basal adenovirus E1b TATA element
    

    Use

    Entry vector

    Available Since

    Aug. 11, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLenti CRISPR V2 sgShmt2_1

    Plasmid

    #106314

    Purpose

    Express Cas9 and sgRNA targeting mShmt2

    Depositor

    Richard Possemato

    Article

    Minton et al Mol Cell. 2018 Feb 15;69(4):610-621.e5.

    Insert

    sgRNA targeting SHMT2 (Shmt1 Mouse)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    April 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLenti CRISPR V2 sgShmt2_2

    Plasmid

    #106315

    Purpose

    Express Cas9 and sgRNA targeting mShmt2

    Depositor

    Richard Possemato

    Article

    Minton et al Mol Cell. 2018 Feb 15;69(4):610-621.e5.

    Insert

    sgRNA targeting mShmt2
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    April 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. lentiCRISPR v2 kif26b sgRNA1

    Plasmid

    #102857

    Purpose

    Expresses sgRNA targeting mouse Kif26b

    Depositor

    Henry Ho

    Article

    Susman et al Elife. 2017 Sep 8;6. pii: e26509. doi: 1

    Insert

    Mouse Kif26b (Kif26b Mouse)
    

    Use

    Lentiviral

    Available Since

    April 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. lentiCRISPR v2 kif26b sgRNA2

    Plasmid

    #102858

    Purpose

    Expresses sgRNA targeting mouse Kif26b

    Depositor

    Henry Ho

    Article

    Susman et al Elife. 2017 Sep 8;6. pii: e26509. doi: 1

    Insert

    Mouse Kif26b (Kif26b Mouse)
    

    Use

    Lentiviral

    Available Since

    Dec. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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