We narrowed to 2,466 results for: crispr system

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pAAV-FLEXfrt-SaCas9-U6-sgSlc17a8

Plasmid

#124861

Purpose

Mutagenesis of Slc17a8

Depositor

Larry Zweifel

Article

Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

Insert

Slc17a8 (Slc17a8 Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Available Since

May 3, 2019

Availability

Academic Institutions and Nonprofits only

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pVCERC-5 Pvan-dcas9 (Sth3)

Plasmid

#133319

Purpose

expresses dcas9 from Streptococcus thermophilus #3; induced with vanillate; for homologous recombination at the vanillate locus in Caulobacter crescentus; tetracycline resistance

Depositor

Michael Laub

Article

Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:

Insert

dcas9 (Streptococcus thermophilus #3)

Use

Unspecified

Promoter

vanillate

Available Since

Jan. 30, 2020

Availability

Academic Institutions and Nonprofits only

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CMV-AsCas12f-v4.1

Plasmid

#204636

Purpose

Expression of AsCas12f v4.1 in mammalian cells

Depositor

Weixin Tang

Article

Wu et al Nat Chem Biol. 2023 Jul 3. doi: 10.1038/

Insert

AsCas12f-v4.1

Tags

NLS

Expression

Mammalian

Mutation

D169K, N199K, N328G, D364R

Promoter

CMV

Available Since

Aug. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pYPQ262m3

Plasmid

#149556

Purpose

Gateway entry clone for CRISPR-iSpyMacCas9 ABE7.10 A-G base editing at NAAR PAMs

Depositor

Yiping Qi

Article

Sretenovic et al Plant Commun. 2020 Jul 22;2(2):100101. d

Insert

ecTadAwt-ecTadAmut-ziSpyMacCas9(D10A)

Use

CRISPR

Expression

Plant

Mutation

D10A, R221K, N394K, Protospacer interacting domai…

Available Since

July 20, 2020

Availability

Academic Institutions and Nonprofits only

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pM423

Plasmid

#172408

Purpose

Expresses sfGFP (1-10) and MCP-GFP11

Depositor

Hajin Kim

Article

Chaudhary et al Genome Res. 2020 Sep;30(9):1306-1316. do

Insert

MCP, bipartite sfGFP

Use

CRISPR and Lentiviral

Tags

sfGFP {1-10}

Expression

Mammalian

Promoter

hPGK

Available Since

Sept. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pXGFPC-5 Pxyl-dcas9 (Spa)

Plasmid

#133318

Purpose

expresses dcas9 (Streptococcus pasteurianus); repressed with 0.2% glucose, induced with 0.3% xylose; for homologous recombination at the xylose locus in Caulobacter crescentus; tetracycline resistance

Depositor

Michael Laub

Article

Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:

Insert

dcas9 (Streptococcus pasteurianus)

Use

Unspecified

Promoter

xylose

Available Since

Feb. 5, 2020

Availability

Academic Institutions and Nonprofits only

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psgRNA2.02-PP7-ccdB

Plasmid

#99912

Purpose

Basic cloning vector for the expression of PP7 inserted sgRNA

Depositor

Yujie Sun

Article

Shao et al Nucleic Acids Res. 2016 May 19;44(9):e86

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available Since

Oct. 10, 2017

Availability

Academic Institutions and Nonprofits only

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psgRNA2.02-MS2-ccdB

Plasmid

#99911

Purpose

Basic cloning vector for the expression of MS2 inserted sgRNA

Depositor

Yujie Sun

Article

Shao et al Nucleic Acids Res. 2016 May 19;44(9):e86

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available Since

Oct. 10, 2017

Availability

Academic Institutions and Nonprofits only

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EFS eGFP hPGK BSD

Plasmid

#188520

Purpose

Backbone for CRISPR interference (CRISPRi) isogenic PAM testing

Depositor

Isaac Hilton

Article

Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

Type

Empty backbone

Use

Lentiviral

Tags

eGFP

Expression

Mammalian

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pBXMCS-2 Pconstitutive-sgRNA(Spa)_ctrA

Plasmid

#133342

Purpose

for constitutive expression of a single guide RNA from Streptococcus pasteurianus with a seed region that targets ctrA gene's promoter from Caulobacter crescentus

Depositor

Michael Laub

Article

Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:

Insert

sgRNA_ctrA

Use

CRISPR

Promoter

constitutive

Available Since

Jan. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pBXMCS-2 Pconstitutive-sgRNA(Sth3)_gcrA1

Plasmid

#133340

Purpose

for constitutive expression of a single guide RNA from Streptococcus thermophilus #3 with a seed region that targets gcrA gene's promoter on the non-template strand in Caulobacter crescentus

Depositor

Michael Laub

Article

Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:

Insert

sgRNA_gcrA

Use

CRISPR

Promoter

constitutive

Available Since

Jan. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pHAGE-IRES-puro-NLS-dHgm4Cas13b-EGFP-NLS

Plasmid

#191360

Purpose

Overexpression of NLS-dHgm4Cas13b-EGFP-NLS in human cells

Depositor

Ling-Ling Chen

Article

Yang et al Cell Insight. 2022 Jun 15;1(4):100044. d

Insert

NLS-dHgm4Cas13b-EGFP-NLS

Tags

EGFP

Expression

Mammalian

Available Since

Oct. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pBXMCS-2 Pconstitutive-sgRNA(Spy)_ctrA

Plasmid

#133334

Purpose

for constitutive expression of a single guide RNA from Streptococcus pyogenes with a seed region that targets ctrA gene's promoter from Caulobacter crescentus

Depositor

Michael Laub

Article

Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:

Insert

sgRNA_ctrA

Use

CRISPR

Promoter

constitutive

Available Since

Jan. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pET-3a-His-MBP-TEV-VC_Cas1_WT

Plasmid

#120364

Purpose

Expresses wildtype V-C Cas1

Depositor

Jennifer Doudna

Article

Wright et al Mol Cell. 2019 Feb 21;73(4):727-737.e3.

Insert

VC_Cas1

Use

CRISPR

Expression

Bacterial

Available Since

April 12, 2021

Availability

Academic Institutions and Nonprofits only

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pET-3a-His-MBP-TEV-VD_Cas1_WT

Plasmid

#120365

Purpose

Expresses wildtype V-D Cas1

Depositor

Jennifer Doudna

Article

Wright et al Mol Cell. 2019 Feb 21;73(4):727-737.e3.

Insert

VD_Cas1

Use

CRISPR

Expression

Bacterial

Available Since

April 12, 2021

Availability

Academic Institutions and Nonprofits only

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pYPQ141C2.0

Plasmid

#99905

Purpose

Express single gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under OsU6 promoter

Depositor

Yiping Qi

Article

Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(

Type

Empty backbone

Use

CRISPR

Expression

Plant

Promoter

OsU6

Available Since

March 8, 2018

Availability

Academic Institutions and Nonprofits only

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pM215A

Plasmid

#173217

Purpose

Expresses ScFV-GCN4-sFGFP10M2-GB1

Depositor

Hajin Kim

Article

Chaudhary et al Genome Res. 2020 Sep;30(9):1306-1316. do

Insert

ScFV-GCN4-sfGFP10M2-GB1

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pM214

Plasmid

#173216

Purpose

Expresses MCP-mCherry-GFP11-GB1

Depositor

Hajin Kim

Article

Chaudhary et al Genome Res. 2020 Sep;30(9):1306-1316. do

Insert

MCP-mCherry-GFP11-GB1

Use

CRISPR and Lentiviral

Tags

HA and mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 31, 2021

Availability

Academic Institutions and Nonprofits only

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pBXMCS-2 Pconstitutive-sgRNA(Sth3)_cpaA

Plasmid

#133344

Purpose

for constitutive expression of a single guide RNA from Streptococcus thermophilus #3 with a seed region that targets cpaA gene's promoter from Caulobacter crescentus

Depositor

Michael Laub

Article

Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:

Insert

sgRNA_cpaA

Use

CRISPR

Promoter

constitutive

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pENTR-R4R3-stop

Plasmid

#113745

Purpose

Contains three stop codons in every frame to ensure a premature stop codon when introduced into the genome via homology-directed repair. Gateway multisite entry clone.

Depositor

Magdalena Bezanilla

Article

Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

Insert

stop cassette

Use

CRISPR; Gateway entry vector

Mutation

Added 3 stop codons to the original sequence; see…

Available Since

Sept. 24, 2018

Availability

Academic Institutions and Nonprofits only

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