We narrowed to 5,654 results for: pCas

1. LLP784_SnoopCatcher-DNMT3A-CO

   Plasmid

   #211777

   Purpose

   SnoopTag counterpart binding domain, SnoopCatcher, fused to DNA methyltransferase DNMT3A, with GFP selection

   Depositor

   Ryan Lister

   Article

   Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

   Insert

   Spy-EZH2
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Last 300 bp codon-optimised (CO) to detect EZH2 e…

   Promoter

   pEF1a and pSV40

   Available since

   Dec. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAG-EnvARGCD

   Plasmid

   #100800

   Purpose

   express fusion protein of EnvA (ASLV-A envelope protein) extracellular and transmembrane domains with rabies virus SAD B19 strain glycoprotein intracellular domain

   Depositor

   Ian Wickersham

   Insert

   fusion protein of EnvA extracellular and transmembrane domain with rabies virus glycoprotein intracellular domain
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Dec. 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCas_5+7_gfp

   Plasmid

   #60595

   Purpose

   encodes a reversed Int7 gene flanked by the attB/P sites of Int5, as well as a reversed GFP (GFPmut33) gene flanked by the attB/P sites of Int7.

   Depositor

   Christopher Voigt

   Article

   Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

   Insert

   Cas_5+7_gfp
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAG-tdTHC

   Plasmid

   #112621

   Purpose

   expression plasmid with CAG promoter driving multicistronic cassette H2BtdTomato_p2A_HygroR_p2A_CreERt2

   Depositor

   Stefan Heller

   Article

   Hartman et al Dev Biol. 2018 Sep 1. pii: S0012-1606(18

   Inserts

   H2B-tdTomato (H2BC3 Human, Anthozoa)
   Hygrophosphotransferase
   Cre-ERT2 fusion protein
   

   Use

   Cre/Lox

   Tags

   tdTomato

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Sept. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCas9-VRER_2A_GFP

   Plasmid

   #75475

   Purpose

   Cas9 VRER variant that detects NGCG PAM, combined with 2A_GFP for expression control

   Depositor

   Marc Tessier-Lavigne

   Article

   Paquet et al Nature. 2016 May 5;533(7601):125-9. doi:

   Insert

   Cas9-VRER
   

   Use

   CRISPR

   Tags

   2A_GFP

   Expression

   Mammalian

   Mutation

   D1135V, G1218R, R1335E, T1337R

   Promoter

   CAG

   Available since

   May 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAG-B19M

   Plasmid

   #59923

   Purpose

   Expression vector for Rabies virus SAD B19 matrix protein gene

   Depositor

   Ian Wickersham

   Insert

   Rabies virus matrix protein
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Sept. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCaRNA3-eIFiso4E120(+)

   Plasmid

   #86092

   Purpose

   This vector is used to specifically silence the eIF(iso)4E gene in peach via VIGS.

   Depositor

   Aiming Wang

   Article

   Cui et al Plant Biotechnol J. 2016 Aug 27. doi: 10

   Insert

   eIFiso4E
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Jan. 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAG-Bmp2

   Plasmid

   #163587

   Purpose

   Expresses BMP-2 in mammalian cells

   Depositor

   Takeshi Imai

   Article

   Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

   Insert

   Bmp2 (Bmp2 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAGGS-6020nes

   Plasmid

   #108656

   Purpose

   A JNK biosensor based on fluorescence resonance energy transfer (FRET) and those based on bioluminescence energy transfer (BRET)

   Depositor

   Michiyuki Matsuda

   Article

   Komatsu et al Sci Rep. 2018 Jun 12;8(1):8984. doi: 10.

   Insert

   hyBRET-JNK-EV
   

   Use

   Tags

   XFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAG-VHC

    Plasmid

    #112618

    Purpose

    expression plasmid with CAG promoter driving multicistronic cassette H2BVenus_p2A_HygroR_p2A_CreERt2

    Depositor

    Stefan Heller

    Article

    Hartman et al Dev Biol. 2018 Sep 1. pii: S0012-1606(18

    Inserts

    H2B-Venus (H2BC3 Aequorea victoria, Human)
    Hygrophosphotransferase
    Cre-ERT2 fusion protein
    

    Use

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Sept. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAGGS-ROSAf

    Plasmid

    #54323

    Purpose

    TALEN expression construct for targeting the murine ROSA26 locus. It recognize the sequence: CTGCAACTCCAGTCT. Can be used with the pBS SK mCherryROSAbsr plasmid

    Depositor

    Silvestro Conticello

    Insert

    TALEN for the murine ROSA26 locus
    

    Use

    Mouse Targeting and TALEN

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAGG

    Available since

    Sept. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAG-GFPmG1

    Plasmid

    #50444

    Purpose

    Expression of a EGFP variant in mammalian cells

    Depositor

    Connie Cepko

    Article

    Tang et al Cell. 2013 Aug 15;154(4):928-39. doi: 10

    Insert

    GFPmG1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    E143K, N147Q

    Promoter

    CAG

    Available since

    March 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. LLP787_SnoopCatcher-EZH2-FL-CO

    Plasmid

    #211780

    Purpose

    SnoopTag counterpart binding domain, SnoopCatcher, fused to polycomb repressive complex subunit EZH2, with GFP selection

    Depositor

    Ryan Lister

    Article

    Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

    Insert

    aGCN4-DNMT3A
    

    Use

    Tags

    3xTy1

    Expression

    Mammalian

    Mutation

    Last 300 bp codon-optimised (CO) to detect DNMT3A…

    Promoter

    pEF1a and pSV40

    Available since

    Dec. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSL2622 (pCas7_Vch)

    Plasmid

    #200894

    Purpose

    Expresses human codon-optimized V. cholerae Cas7 from a CMV promoter.

    Depositor

    Samuel H. Sternberg

    Article

    Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

    Insert

    VchCas7
    

    Use

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCAG-mTcte1-PA

    Plasmid

    #176469

    Purpose

    Expression vector of mouse t-complex-associated testis expressed 1 (Tcte1) tagged with PA at C-terminus.

    Depositor

    Masahito Ikawa

    Article

    Morohoshi et al PLoS Genet. 2020 Jan 21;16(1):e1008585.

    Insert

    t-complex-associated testis expressed 1 (Tcte1 Mouse)
    

    Use

    Tags

    PA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAGGS-AcGFP-C

    Plasmid

    #203325

    Purpose

    Express N-terminal AcGFP-fused protein in mammalian cell

    Depositor

    Chiaki Murakami

    Article

    Furuta et al FEBS Open Bio. 2023 May 11. doi: 10.1002

    Type

    Empty backbone

    Use

    Tags

    AcGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCAGGS-ROSAr

    Plasmid

    #54324

    Purpose

    TALEN expression construct for targeting the murine ROSA26 locus. It recognize the sequence: GGGAGTCTTCTGGGC. Can be used with the pBS SK mCherryROSAbsr plasmid

    Depositor

    Silvestro Conticello

    Insert

    TALEN for the murine ROSA26 locus
    

    Use

    Mouse Targeting and TALEN

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAGG

    Available since

    Sept. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAG-HArcLight

    Plasmid

    #138326

    Purpose

    chemigenetic voltage indicator

    Depositor

    Eric Schreiter

    Article

    Deo et al Nat Chem Biol. 2021 Jun;17(6):718-723. d

    Insert

    HArcLight
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    March 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAG-Bmp5

    Plasmid

    #163589

    Purpose

    Expresses BMP-5 in mammalian cells

    Depositor

    Takeshi Imai

    Article

    Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

    Insert

    Bmp5 (Bmp5 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMA-SpCas9-g9

    Plasmid

    #80792

    Purpose

    BsaI-based cloning of SpCas9 gRNA guide sequence, position 9/19/29 in the array

    Depositor

    Yonglun Luo

    Article

    Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.

    Insert

    CRISPR gRNA expression cassette (for SpCas9)
    

    Use

    CRISPR

    Tags

    na

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Sept. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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