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miniTurbo-V5_pRS415

Plasmid

#107168

Purpose

expresses V5-tagged miniTurbo in the yeast cytosol

Depositor

Alice Ting

Article

Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

Insert

miniTurbo (BirA mutant)

Use

Tags

Expression

Yeast

Mutation

aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

Promoter

Available since

May 4, 2018

Availability

Academic Institutions and Nonprofits only

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pEFmEGFP-mDia2

Plasmid

#25407

Depositor

Arthur Alberts

Article

Tominaga et al Mol Cell. 2000 Jan . 5(1):13-25.

Insert

mDia2 (Diaph3 Mouse)

Use

Tags

EGFP, His, and Myc

Expression

Mammalian

Mutation

Encodes mDia2

Promoter

Available since

Aug. 24, 2010

Availability

Academic Institutions and Nonprofits only

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pCEP4-FLAG-Tapasin

Plasmid

#141308

Purpose

Mammalian expression of FLAG-tagged Tapasin

Depositor

Erik Procko

Article

McShan et al Nat Commun. 2021 May 26;12(1):3174. doi:

Insert

TAPBP (TAPBP Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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pHAGE-TO-nls-st1dCas9-3nls-3XGFP

Plasmid

#64111

Purpose

Expression of St1 dCas9-3XGFP in mammalian cells

Depositor

Thoru Pederson

Article

Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

Insert

St1 dCas9

Use

CRISPR and Lentiviral

Tags

3XsfGFP

Expression

Mammalian

Mutation

Promoter

CMV-TO

Available since

April 24, 2015

Availability

Academic Institutions and Nonprofits only

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IMS-APEX2

Plasmid

#79058

Purpose

Expresses APEX in the mitochondrial intermembrane space.

Depositor

Alice Ting

Article

Hung et al Mol Cell. 2014 Jul 17;55(2):332-41. doi:

Insert

APEX

Use

Tags

V5

Expression

Bacterial and Mammalian

Mutation

Promoter

T7 promoter

Available since

Aug. 12, 2016

Availability

Academic Institutions and Nonprofits only

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Flag-TurboID-NES_pLX304

Plasmid

#202008

Purpose

expresses TurboID in the mammalian cytosol, lentiviral vector

Depositor

Alice Ting

Article

Qin et al Cell. 2023 Jun 23:S0092-8674(23)00596-2.

Insert

Flag-TurboID-NES

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 29, 2023

Availability

Academic Institutions and Nonprofits only

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pHAGE EF1α dCas9-KRAB

Plasmid

#50919

Purpose

Constitutive dCas9-KRAB 2nd generation lentiviral expression vector

Depositor

Scot Wolfe, Rene Maehr

Article

Kearns et al Development. 2014 Jan;141(1):219-23. doi

Insert

dCas9

Use

CRISPR and Lentiviral

Tags

3xHA and KRAB

Expression

Mammalian

Mutation

D10A, H840A

Promoter

EF1alpha

Available since

March 28, 2014

Availability

Academic Institutions and Nonprofits only

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pLYS1-FLAG-MitoGFP-HA

Plasmid

#50057

Purpose

Expression of GFP targeted to mitochondria with Flag and HA tags

Depositor

Vamsi Mootha

Article

Sancak et al Science. 2013 Nov 14.

Insert

Mito-GFP

Use

Lentiviral

Tags

Flag and HA

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 13, 2014

Availability

Academic Institutions and Nonprofits only

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pCRII_TOPO_hNEAT1

Plasmid

#61518

Purpose

human NEAT1_v1 short isoform 3.7kb insert in general cloning vector

Depositor

Archa Fox

Article

Clemson et al Mol Cell. 2009 Mar 27;33(6):717-26. doi:

Insert

NEAT1 (NEAT1 Human)

Use

General cloning vector

Tags

Expression

Mutation

*see comment below

Promoter

Available since

March 11, 2015

Availability

Academic Institutions and Nonprofits only

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pT22-GluCNZ-6His

Plasmid

#213504

Purpose

Designed for cytoplasmic E.coli overexpression of glucanase (β-1,3-glycosidase) for gentle yeast spheroblasting that unlike Zymolyase prevents proteolysis of intracellular components.

Depositor

John Denu

Article

Kuznetsov et al J Biol Chem. 2018 Aug 3;293(31):12271-12

Insert

Glucanase (glucan endo-1,3-beta-glucosidase)

Use

Tags

6His tag

Expression

Bacterial

Mutation

1-36 Signal Sequence Deletion

Promoter

T7

Available since

March 11, 2024

Availability

Academic Institutions and Nonprofits only

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pWZL Blast mouse E-cadherin

Plasmid

#18804

Depositor

Bob Weinberg

Article

Onder et al Cancer Res. 2008 May 15. 68(10):3645-54.

Insert

E-cadherin (Cdh1 Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 7, 2008

Availability

Academic Institutions and Nonprofits only

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pT3-EF1α-Dest

Plasmid

#133299

Purpose

The pT3-EF1α destination vector without any insert which can be used for LR reaction.

Depositor

Xin Chen

Article

Lee et al Hepatology. 2008 Apr;47(4):1200-10. doi:

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

EF1α

Available since

July 20, 2022

Availability

Academic Institutions and Nonprofits only

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Dendra2-Actin-C-18

Plasmid

#57701

Purpose

Localization: Actin, Excitation: 490 / 553, Emission: 507 / 573

Depositor

Michael Davidson

Article

McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

Insert

Actin (ACTB Human)

Use

Tags

Dendra2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 6, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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Dendra2-Golgi-7

Plasmid

#57722

Purpose

Localization: Golgi, Excitation: 490 / 553, Emission: 507 / 573

Depositor

Michael Davidson

Article

McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

Insert

Golgi (B4GALT1 Human)

Use

Tags

Dendra2

Expression

Mammalian

Mutation

aa 1-82 of NM_001497.3

Promoter

CMV

Available since

Jan. 6, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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MSCV_JAK3_IRES_BFP

Plasmid

#206291

Purpose

Expresses the wild type form of human JAK3.

Depositor

Charles de Bock

Article

Omari et al Heliyon. 2023 Nov 8;9(11):e22085. doi: 1

Insert

JAK3 (JAK3 Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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FSW-HRP-TM

Plasmid

#82540

Purpose

Lentiviral vector for expressing HRP-TM in neurons

Depositor

Alice Ting

Article

Loh et al Cell. 2016 Aug 25;166(5):1295-1307.e21.

Insert

HRP fused to TM domain of PDGFR

Use

Lentiviral

Tags

HRP

Expression

Mammalian

Mutation

Promoter

Human Synapsin

Available since

Nov. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pAG426GPD-ccdB-yeastSMASh-YFP

Plasmid

#68854

Purpose

Expresses SMASh tag fused to YFP at N terminus in yeast

Depositor

Michael Lin

Article

Chung et al Nat Chem Biol. 2015 Jul 27. doi: 10.1038

Insert

yeastSMASh-YFP

Use

Tags

SMASh tag

Expression

Yeast

Mutation

Promoter

GPD

Available since

Sept. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pT2/shp53/GFP4

Plasmid

#20208

Depositor

John Ohlfest

Article

Wiesner et al Cancer Res. 2009 Jan 15. 69(2):431-9.

Insert

shp53/GFP4

Use

Transposon

Tags

Expression

Mutation

Promoter

Available since

Dec. 3, 2009

Availability

Academic Institutions and Nonprofits only

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pET28-mE1

Plasmid

#32534

Depositor

Jorge Eduardo Azevedo

Article

Carvalho et al Mol Biotechnol. 2011 Oct 20.

Insert

E1, ubiquitin-activating enzyme (Uba1 Mouse)

Use

Tags

His tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 18, 2011

Availability

Academic Institutions and Nonprofits only

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PB-Mycn

Plasmid

#60667

Purpose

PiggyBac expression vector encoding murine Mycn downstream of a doxycycline inducible promoter

Depositor

James Thomson

Article

Vereide et al Stem Cell Reports. 2014 Dec 9;3(6):1043-

Insert

Mycn (Mycn Mouse)

Use

Piggybac

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 15, 2016

Availability

Academic Institutions and Nonprofits only

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