Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

Filter By

We narrowed to 40,940 results for: Ina

Showing: 6781 - 6800 of 40940 results

  1. pcPylRS(Y384F Y306A)

    Plasmid
    #196486
    Purpose
    To express Methanosarcina mazei PylRS(Y384F Y306A) and allow UAG codon to be translated into Z-Lys or Azido-Z-Lys when used together with the pU6tRNAPyl plasmid.
    Depositor
    Kensaku Sakamoto
    Article
    Hino et al Methods Mol Biol. 2012;794:215-28. doi:
    Insert
    pyrrolysyl-tRNA synthetase variant with Y384F Y306A mutations
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    March 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    196486-plasmid-map-sequence-id-386704

    Enlarge

  2. pSUMO-YHRC

    Plasmid
    #54336
    Purpose
    Expression vector (pT7) for untagged expression as well as fusions to His6-SUMO or His6 tags. Designed for single or multiple fragment cloning by yeast homologous recombination.
    Depositor
    Claes Andréasson
    Article
    Holmberg et al Protein Expr Purif. 2014 Jun;98:38-45. d
    Type
    Empty backbone
    Use
    Tags
    6His-SUMO (yeast gene: SMT3)
    Expression
    Bacterial
    Mutation
    Promoter
    T7-LacO
    Available since
    Aug. 1, 2014
    Availability
    Academic Institutions and Nonprofits only
    54336-plasmid-map-sequence-id-84530

    Enlarge

  7. pOCC65

    Plasmid
    #118865
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C protease, and N-terminal monomeric GFP
    Depositor
    PEPC MPI CBG, Marc Bickle
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-HIS6-3C-mGFP-NotI-ccdB-AscI-stop-HindIII cassette
    Use
    Tags
    HIS6, cleavable with 3C protease, mGFP
    Expression
    Insect
    Mutation
    Promoter
    polH
    Available since
    Jan. 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    118865-plasmid-map-sequence-id-232317

    Enlarge

  8. pCAG-C-CreintG

    Plasmid
    #69573
    Purpose
    One half component of the optimized Cre recombinase dependent on GFP (CRE-DOG OPT) for expression in mammalian cells
    Depositor
    Connie Cepko
    Article
    Tang et al Nat Neurosci. 2015 Sep;18(9):1334-41. do
    Insert
    kozak consensus - C-CreintG
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    Nov. 13, 2015
    Availability
    Academic Institutions and Nonprofits only
    69573-plasmid-map-sequence-id-129736

    Enlarge

  9. p663-UBC-miniTurbo-V5-CDK13_IDG-K

    Plasmid
    #135266
    Purpose
    Gateway destination clone of CDK13 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    miniTurbo-V5-CDK13 (CDK13 Human)
    Use
    Lentiviral; Gateway destination
    Tags
    V5-miniTurbo
    Expression
    Mammalian
    Mutation
    Promoter
    Ubiquitin
    Available since
    Jan. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    135266-plasmid-map-sequence-id-268080

    Enlarge

  14. pOCC292

    Plasmid
    #118881
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with C-terminal mRuby3, and C-terminal HIS6 tag, cleavable with 3C
    Depositor
    PEPC MPI CBG, Marc Bickle
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-NotI-ccdB-AscI-mRuby3-3C-HIS6-stop-HindIII cassette
    Use
    Tags
    mRuby3, HIS6, cleavable with 3C protease
    Expression
    Insect
    Mutation
    Promoter
    polH
    Available since
    Jan. 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    118881-plasmid-map-sequence-id-232308

    Enlarge

  15. p667-UBC-AKT1-V5-miniTurbo_IDG-K

    Plasmid
    #189889
    Purpose
    Gateway destination clone of AKT1 (human) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    AKT1 (AKT1 Human)
    Use
    Lentiviral; Gateway destination
    Tags
    V5-miniTurbo
    Expression
    Mammalian
    Mutation
    Promoter
    Ubiquitin
    Available since
    Oct. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    189889-plasmid-map-sequence-id-374470

    Enlarge

  16. p663-UBC-miniTurbo-V5-AKT1_IDG-K

    Plasmid
    #189890
    Purpose
    Gateway destination clone of AKT1 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    AKT1 (AKT1 Human)
    Use
    Lentiviral; Gateway destination
    Tags
    miniTurbo-V5
    Expression
    Mammalian
    Mutation
    Promoter
    Ubiquitin
    Available since
    Oct. 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    189890-plasmid-map-sequence-id-374279

    Enlarge

  17. pOCC291

    Plasmid
    #118867
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C, and N-terminal mRuby3
    Depositor
    PEPC MPI CBG, Marc Bickle
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-HIS6-3C-mRuby3-NotI-ccdB-AscI-stop-HindIII cassette
    Use
    Tags
    HIS6, cleavable with 3C protease, mRuby3
    Expression
    Insect
    Mutation
    Promoter
    polH
    Available since
    Jan. 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    118867-plasmid-map-sequence-id-232275

    Enlarge

  18. pYZ172

    Plasmid
    #98409
    Purpose
    Donor DNA plasmid to introduce lys9 deletion in S. pombe. Linearization with Not1 digestion.
    Depositor
    Jef Boeke
    Article
    Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.
    Inserts
    upstream homologous region to delete lys9 in genome by recombination
    downstream homologous region to delete lys9 in genome by recombination
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    98409-plasmid-map-sequence-id-198900

    Enlarge

Showing: 6781 - 6800 of 40940 results