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We narrowed to 62,186 results for: cloning

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Showing: 6781 - 6800 of 62186 results

  3. pDG335

    Plasmid
    #100899
    Purpose
    SpCas9n (D10A Nickase mutant) with a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of DSBs with no off-target cuts.
    Depositor
    Paul Thomas
    Article
    Adikusuma et al PLoS One. 2017 Dec 6;12(12):e0187236. do
    Inserts
    humanized CRISPR associated protein 9 Nickase
    U6-gRNA scaffold 1
    U6-gRNA scaffold 2
    Use
    CRISPR and Mouse Targeting
    Tags
    HA
    Expression
    Mammalian
    Mutation
    D10A mutant converts to Nickase
    Promoter
    CBh and U6
    Available Since
    Dec. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    100899-plasmid-map-sequence-id-195324

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  4. WDR39

    Plasmid
    #25308
    Purpose
    Expresses His-tagged WDR39 (CIAO1) in bacteria
    Depositor
    Cheryl Arrowsmith
    Insert
    WDR39_1 (CIAO1 Human)
    Tags
    6xHis
    Expression
    Bacterial
    Promoter
    T7-lacO
    Available Since
    Aug. 30, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits
    25308-plasmid-map-sequence-id-139124

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  5. pDONR P2R-P3-IRES_ECFP

    Plasmid
    #48354
    Purpose
    Contributes an IRES-ECFP cassette as the 3’-module during MultiSite Gateway cloning of a bi-cistronic mRNA shared with a two-part fusion protein encoded by the 5′- and middle modules.
    Depositor
    Anna Planas, Tomas Santalucia
    Article
    Buj et al BMC Mol Biol. 2013 Aug 20;14(1):18. doi:
    Insert
    IRES_ECFP
    Use
    Gateway Cloning
    Mutation
    ECFP translation is mediated by an IRES. Contains…
    Promoter
    none
    Available Since
    April 9, 2014
    Availability
    Academic Institutions and Nonprofits only
    48354-plasmid-map-sequence-id-70631

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  6. TBRII-C-18

    Plasmid
    #62797
    Purpose
    Localization: Membrane Receptor, C1 Cloning Vector, Excitation:N/A, Emission:N/A
    Depositor
    Michael Davidson
    Insert
    TBRII (TGFBR2 Human)
    Tags
    SNAP Tag
    Expression
    Mammalian
    Mutation
    H385N
    Promoter
    CMV
    Available Since
    March 12, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits
    62797-plasmid-map-sequence-id-173039

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  7. pLIX403-ccdB-EYFP

    Plasmid
    #158564
    Purpose
    Set of empty Gateway Cloning compatible, inducible, lentiviral vectors with various mammalian selection markers and N-terminal fluorescent protein fusions
    Depositor
    Alejandro Chavez
    Type
    Empty backbone
    Use
    Lentiviral
    Expression
    Mammalian
    Available Since
    Aug. 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    158564-plasmid-map-sequence-id-309879

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  8. pLIX403-EBFP2-ccdB-G418

    Plasmid
    #158556
    Purpose
    Set of empty Gateway Cloning compatible, inducible, lentiviral vectors with various mammalian selection markers and N-terminal fluorescent protein fusions
    Depositor
    Alejandro Chavez
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    EBFP2
    Expression
    Mammalian
    Available Since
    Aug. 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    158556-plasmid-map-sequence-id-309869

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  10. pB6-Hipp11-DTA-Capecchi-for

    Plasmid
    #126649
    Purpose
    B6J Hipp11 targeting vector homology arms, Capecchi configuration, DTA, I-CeuI, forward cloning sites, low copy number
    Depositor
    Mario Capecchi
    Type
    Empty backbone
    Use
    Mouse Targeting
    Available Since
    June 26, 2019
    Availability
    Academic Institutions and Nonprofits only
    126649-plasmid-map-sequence-id-247127

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  11. Hsp68-LacZ-Gibson

    Plasmid
    #170102
    Purpose
    Transgenic LacZ reporter construct for validation of enhancer sequences (inserted via Gibson-cloning). Can be introduced into the mouse genome via random integration following pro-nuclear injection.
    Depositor
    Len Pennacchio
    Type
    Empty backbone
    Use
    Mouse Targeting
    Promoter
    Mouse Hsp68 minimal promoter
    Available Since
    Oct. 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    170102-plasmid-map-sequence-id-346308

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  12. mRaspberry-C1

    Plasmid
    #54659
    Purpose
    Localization: C1 Cloning Vector, Excitation: 598, Emission: 625
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Tags
    mRaspberry
    Expression
    Mammalian
    Available Since
    June 11, 2014
    Availability
    Academic Institutions and Nonprofits only
Showing: 6781 - 6800 of 62186 results