We narrowed to 10,841 results for: esp
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Plasmid#206840PurposeThe plasmid contains the human WIPI1 promotor sequence (-1226 bp upstream to +329 bp downstream of the transcription start site) cloned into pGL4.23[luc2/minP] (NheI/HindIII).DepositorInsertWIPI1 promotor (partial)
UseLuciferaseAvailable SinceJan. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pENTR1A 2xHA GPR137
Plasmid#226809PurposeGateway entry vector for GPR137DepositorInsertGPR137 (GPR137 Human)
UseGateway CloningTags2xHA tag followed by a GDPPVAT linkerMutationSequence optimized from NP_001164351Available SinceJan. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV_GR-RE_MLPmin_BC1093-luc2
Plasmid#227113PurposeGlucocorticoid receptor response element for luciferase and barcode assays; barcode BC1093DepositorInsert12x clustered GR response element linked to MLP minimal promoter driving barcode 1093 and a luciferase reporter gene
UseAAVExpressionMammalianAvailable SinceNov. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcs2-Δhel1/Δhel2HRI-GFP-IRES-mCherry
Plasmid#226101PurposeHRI stability reporter construct with deletion of SIFI degron helices 1&2 for transient expression in mammalian cellsDepositorInsertHRI (EIF2AK1 Human)
TagsGFPExpressionMammalianMutationSIFI degron helices 1 & 2 deleted (Δ61-112)Available SinceOct. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcs2-N-HRI(1-138)-GFP-IRES-mCherry
Plasmid#226100PurposeHRI N-terminal region (residues 1-138) stability reporter construct for transient expression in mammalian cellsDepositorAvailable SinceOct. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pIRHDGAL43C
Plasmid#218279PurposeIntroduction of a LowTempGAL Turbo module (HD-GAL43C) to accelerate induction of GAL promoter inductionDepositorInsertfcy1(207-356)-pAgTEF1>ble>tAgTEF1-pNRG2>GAL4>tNRG2-PENO2> UBI4>RHGSGTMV>DHFRP66L>G*6>GAL3C(S509P)-tENO2-fcy1(454-778)
ExpressionYeastMutationGAL3 (S509P), DHFR (P66L)Available SinceMay 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pIRHDGAL3C
Plasmid#218276PurposeIntroduction of a LowTempGAL Turbo module (HD-GAL3C) to accelerate induction of GAL promoter inductionDepositorInsertfcy1(207-356)-pAgTEF1>ble>tAgTEF1-pENO2> UBI4>RHGSGTMV>DHFRP66L>G*6>GAL3C(S509P)>tENO2-fcy1(454-778)
ExpressionYeastMutationGAL3 (S509P), DHFR (P66L)Available SinceMay 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBluescript II Ks(+)-ZsGreen1-SV40 PolyA-Stop Mettl3 HDR
Plasmid#207136PurposeDNA sequence source for amplifying an HDR template for mouse Mettl3 knockout and reporter knock-inDepositorInsert5'HDR Mettl3 - ZsGreen- sv40(polyA) - 3'HDR Mettl3 (Mettl3 Mouse)
ExpressionMammalianAvailable SinceDec. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
GFP Cav2.2 D122A pcDNA3
Plasmid#206068Purposeexpression of rabbit Cav2.2 calcium channel with a N-terminal GFP tag and a D122A mutation that disrupts the interaction with ?2?-1DepositorAvailable SinceOct. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
mEos3.2trLATm2allL+A
Plasmid#203750PurposeEncodes the transmembrane domain from human LAT with mEos3.2 fused on the C terminus. Residues within the transmembrane domain mutated to L and A to increase the TMD interfacial surface area with the membrane. Used as a fluorescent plasma membrane probe.DepositorAvailable SinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pXPR_023d-sgCiPOLR2D
Plasmid#202445PurposeExpression of a CRISPRi guide targeting POLR2DDepositorAvailable SinceJuly 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pXPR_003-sgUBR4
Plasmid#202442PurposeExpression of a guide RNA targeting UBR4DepositorAvailable SinceJune 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pXPR_003-sgUBA6
Plasmid#202440PurposeExpression of a guide RNA targeting UBA6DepositorAvailable SinceJune 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET-Duet-6xHis-TEV-Nsp8(76-198)
Plasmid#201024PurposeBacterial expression of codon optimized SARS-CoV-2 nsp8 residues 76-198, with an N-terminus His tag followed by a TEV cleavage site.DepositorInsertnon-structural protein 8 (ORF1ab Synthetic, Severe acute respiratory syndrome coronavirus 2)
Tags6xHis-TEVExpressionBacterialMutationGene insert is codon optimized for Ecoli.PromoterT7/LacOAvailable SinceMay 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pROS_phleo-Tps1/Gsy2
Plasmid#196612PurposeEncoding guide RNAs for the knock out of TPS1 and GSY2 genesDepositorAvailable SinceMarch 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pROS13-Tps2/Gsy1
Plasmid#196613PurposeEncoding guide RNAs for the knock out of TPS2 and GSY1 genesDepositorAvailable SinceMarch 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
GA46
Plasmid#196614PurposePlasmid used for the C-terminal tagging of Atp3 with mCherry and the auxin-inducible degron in yeastDepositorInsertATP3::mCherry-AID(71-114)-NatMX (ATP3 Budding Yeast)
TagsAID(71-114) and mCherryExpressionYeastAvailable SinceMarch 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUGP1.1
Plasmid#196615PurposePlasmid used for the C-terminal tagging of Ugp1 with mCherry and the auxin-inducible degron in yeastDepositorInsertUGP1::mCherry-AID(71-114)-NatMX (UGP1 Budding Yeast)
TagsAID(71-114) and mCherryExpressionYeastPromoterNative promoterAvailable SinceMarch 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
PX459_GRHL1-exon5
Plasmid#177763PurposesgRNA for CRISPR/Cas9-mediated deletion of human GRHL1DepositorInsertGRHL1 (GRHL1 Human)
UseCRISPRAvailable SinceApril 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
PX459_GRHL1-exon8
Plasmid#177764PurposesgRNA for CRISPR/Cas9-mediated deletion of human GRHL1DepositorInsertGRHL1 (GRHL1 Human)
UseCRISPRAvailable SinceApril 5, 2022AvailabilityAcademic Institutions and Nonprofits only