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pcDNA3.1-mPCSK1

Plasmid

#122672

Purpose

Expresses mouse PCSK1 in mammalian cells

Depositor

Liming Pei

Article

Li et al Mol Cell Biol. 2018 Oct 15;38(21). pii:

Insert

proprotein convertase subtilisin/kexin type 1 (PCSK1) (Pcsk1 Mouse)

Expression

Mammalian

Promoter

T7

Available Since

May 16, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-mPCSK2

Plasmid

#122673

Purpose

Expresses mouse PCSK2 in mammalian cells

Depositor

Liming Pei

Article

Li et al Mol Cell Biol. 2018 Oct 15;38(21). pii:

Insert

Mus musculus proprotein convertase subtilisin/kexin type 2 (Pcsk2) (Pcsk2 Mouse)

Expression

Mammalian

Promoter

T7

Available Since

May 16, 2019

Availability

Academic Institutions and Nonprofits only

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pCAG-mTagBFP2

Plasmid

#122373

Purpose

Expresses mTagBFP2 in mammalian cells

Depositor

Hiroshi Ochiai

Article

Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

Insert

mTagBFP2

Expression

Mammalian

Available Since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

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pUB_smFLAG_KDM5B_MS2

Plasmid

#81084

Purpose

Translation reporter: Encodes KDM5B (1544 aa) fused to smFLAG (Spaghetti Monster FLAG: 10 copies of an epitope tag FLAG).

Depositor

Tim Stasevich

Article

Morisaki et al Science. 2016 Jun 17;352(6292):1425-9. d

Insert

smFLAG_KDM5B_24xMS2

Expression

Mammalian

Available Since

Sept. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pBAD-pHlorina

Plasmid

#182763

Purpose

pH-sensitive RFP pHlorina for detection of efferocytosis

Depositor

Robert Campbell

Article

Raymond et al Science. 2022 Mar 11;375(6585):1182-1187

Insert

pHlorina

Expression

Bacterial

Available Since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pFCcGi

Plasmid

#59324

Purpose

Inducible expression of GFP and constitutive expression of mCherry

Depositor

Sophie Helaine, David Holden

Article

Figueira et al MBio. 2013 Apr 16;4(2):e00065. doi: 10.1

Inserts

mCherry
EGFP

Expression

Bacterial

Promoter

Pbad and rpsM

Available Since

Oct. 24, 2014

Availability

Academic Institutions and Nonprofits only

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pMIIS948-CXCR4

Plasmid

#238213

Purpose

TasR tigRNA expression to target CXCR4 under U6 promoter in human cells.

Depositor

Feng Zhang

Article

Faure et al Science. 2025 Feb 27:eadv9789. doi: 10.1

Insert

tigRNA

Expression

Mammalian

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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pSSBIO32_dCas9_RPA1786

Plasmid

#240489

Purpose

CRISPRi-mediated Knockdown of RPA1786

Depositor

Rajib Saha

Article

Kathol et al Appl Environ Microbiol. 2025 Aug 20;91(8

Insert

Cas9 (cas9 Streptococcus pyogenes)

Use

CRISPR

Expression

Bacterial

Mutation

D10A, H840A

Promoter

Plac

Available Since

Aug. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pYC1640

Plasmid

#158719

Purpose

CRISPR system used for genome editing in Mycobacterium tuberculosis. Helper plasmid expresses Sth1 Cas9 and the cognate sgRNA, using zeocin as a selection marker.

Depositor

Yi-Cheng Sun

Article

Yan et al mBio. 2020 Jan 28;11(1). pii: mBio.02364

Insert

Sth1 sgRNA scaffold

Use

CRISPR

Expression

Bacterial

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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gRNA_Cloning Vector

Plasmid

#41824

Purpose

An empty gRNA expression vector, used to create a gRNA to a specific sequence.

Depositor

George Church

Article

Mali et al Science. 2013 Feb 15;339(6121):823-6. do

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

CRISPR

Tags

AflII site for Gibson Assembly

Expression

Mammalian

Available Since

Jan. 11, 2013

Availability

Academic Institutions and Nonprofits only

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pCMV-ABEmax(TadA E59A, TadA*D108Q)

Plasmid

#125655

Purpose

pCMV-ABEmax(TadA E59A, TadA*D108Q) in mammalian cells

Depositor

David Liu

Article

Rees et al Sci Adv. 2019 May 8;5(5):eaax5717. doi:

Insert

ABEmax(TadA E59A TadA* D108Q)

Expression

Mammalian

Available Since

June 6, 2019

Availability

Academic Institutions and Nonprofits only

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NLRP3-mNeonGreen

Plasmid

#200942

Purpose

NLRP3 fused with mNeonGreen to indicate its localization change

Depositor

Nir Hacohen

Article

Liu et al Science. 2023 Aug 4;381(6657):508-514. d

Insert

NLRP3 (NLRP3 Human)

Use

Lentiviral

Available Since

Sept. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pRPF215

Plasmid

#106377

Purpose

C. difficile transposon mutagenesis system. Shuttle vector containing tet-inducible Himar1 transposase and custom ErmR transposon

Depositor

Robert Fagan, Neil Fairweather

Article

Dembek et al MBio. 2015 Feb 24;6(2):e02383. doi: 10.1

Insert

Ptet-himar1-Ter(slpA)-transposon

Use

E. coli - c. difficile shuttle vector

Mutation

Codon optimised for C. difficile

Promoter

Ptet

Available Since

March 8, 2018

Availability

Academic Institutions and Nonprofits only

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pNHEJ-recX-sacB

Plasmid

#158718

Purpose

CRISPR-assisted-NHEJ system used for genome editing in Mycobacterium tuberculosis. Helper plasmid expresses NHEJ machinery of M. marinum and recX.

Depositor

Yi-Cheng Sun

Article

Yan et al mBio. 2020 Jan 28;11(1). pii: mBio.02364

Insert

recX

Expression

Bacterial

Promoter

Pmyc

Available Since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pTrex-n-eGFP

Plasmid

#62544

Purpose

Expression of eGFP in T. cruzi. The NheI-linearized form of this plasmid could be efficiently integrated in to T. cruzi genome

Depositor

Rick Tarleton

Article

Peng et al MBio. 2014 Dec 30;6(1). pii: e02097-14.

Insert

eGFP

Use

(1)trypanosoma cruzi expression of egfp (2) genom…

Tags

None

Mutation

None

Promoter

Ribo-HX1

Available Since

April 16, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pZR069_CROPseq-sgPTPRC-CRISPRi

Plasmid

#180271

Purpose

Lentiviral CROPseq vector expressing PTPRC (CD45) targeting sgRNA for CRISPRi

Depositor

Alexander Marson

Article

Schmidt et al Science. 2022 Feb 4;375(6580):eabj4008.

Insert

PTPRC (CD45) sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pLV-MPAct-mRuby3-IRES-Puro

Plasmid

#155231

Purpose

lentiviral MPAct construct

Depositor

Tobias Meyer

Article

Bisaria et al Science. 2020 Jun 12;368(6496):1205-1210

Insert

Lentiviral expression of MPAct-mRuby sensor

Use

Lentiviral

Tags

mRuby3

Available Since

Aug. 31, 2020

Availability

Academic Institutions and Nonprofits only

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pCEP4-BaLgp160

Plasmid

#100919

Purpose

Mammalian expression plasmid for Env from the BaL HIV-1 isolate

Depositor

Erik Procko

Article

Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

Insert

HIV-1 (BaL) Env

Tags

CD5 leader sequence

Expression

Mammalian

Mutation

Codon-optimized synthetic gene

Promoter

CMV

Available Since

March 25, 2019

Availability

Academic Institutions and Nonprofits only

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pA-His6-Tn5

Plasmid

#171871

Purpose

Expresses protein A and Tn5 Transposase fusion protein in bacterial cells

Depositor

Kaiwei Liang

Article

Wang et al Sci Adv. 2021 Feb 17;7(8). pii: 7/8/eabe

Insert

Protein A and hyperactive Tn5 transposase

Tags

His-tag

Expression

Bacterial

Mutation

WT

Available Since

July 27, 2021

Availability

Academic Institutions and Nonprofits only

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pMBP-LbCas12a

Plasmid

#113431

Purpose

Expresses 10xHis-MBP fused to LbCas12a

Depositor

Jennifer Doudna

Article

Chen et al Science. 2018 Apr 27;360(6387):436-439.

Has Service

Cloning Grade DNA

Insert

LbCas12a

Tags

MBP

Expression

Bacterial

Promoter

T7

Available Since

July 31, 2018

Availability

Academic Institutions and Nonprofits only

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