We narrowed to 63,605 results for: cell

1. LRG-sgRNA.FOXA-ZEB2_CRISPRd_v2

   Plasmid

   #216173

   Purpose

   Express the gRNA targeting the FOXA-binding site in the human ZEB2 locus for the CRISPRd experiment

   Depositor

   Makiko Iwafuchi

   Article

   Matsui et al Mol Cell. 2024 Jan 4:S1097-2765(23)01026

   Insert

   sgRNA- FOXA.bs- ZEB2.locus
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mutation

   Promoter

   Available Since

   July 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. Src(YF)-SsrA(Y7I)-mCer3

   Plasmid

   #220999

   Purpose

   this is microTag (Y7I mutation in SsrA)-Src(Y529F) with mCerulean3 at C-terminal

   Depositor

   Klaus Hahn

   Article

   Liu et al Cell. 2021 Oct 28;184(22):5670-5685.e23.

   Insert

   Src, SsrA, mCerulean3 (Src Mouse)
   

   Use

   Tags

   mCerulean3

   Expression

   Mammalian

   Mutation

   Y529F in Src, Y7I in SsrA

   Promoter

   CMV

   Available Since

   June 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. 2x3APWWP_K299E_D333A-IFP2.0C

   Plasmid

   #215753

   Purpose

   Expresses binding-deficient K299E/D333A mutant of 2xDNMT3A PWWP domain fused to C-terminal part of IFP2.0 as negative control for H3K36me2/3 detection in BiAD sensors

   Depositor

   Albert Jeltsch

   Article

   Kohler et al Cell Rep Methods. 2024 Mar 26:100739. do

   Insert

   2x3APWWP_K299E_D333A-IFP2.0C
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   April 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCF702

   Plasmid

   #209650

   Purpose

   Suicide plasmid carrying a neomycin resistance marker flanked by homologous regions of the Mth60-fimbria encoding operon of Methanothermobacter thermautotrophicus

   Depositor

   Bastian Molitor

   Article

   Fink et al Appl Environ Microbiol. 2023 Jul 26;89(7

   Inserts

   Downstream homologous region
   thermostable neomycin phosphotransferase gene
   Upstream homologous region
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   March 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAS_4xMmaPylT(UUA)_EF1_sfGFP150TAA

   Plasmid

   #174897

   Purpose

   ochre suppression reporter sfGFP150TAA expression, with MmaPylT(UUA) ochre suppressor tRNA cassette

   Depositor

   Simon Elsaesser

   Article

   Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

   Insert

   sf GFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   150TAA in sfGFP

   Promoter

   EF1

   Available Since

   Nov. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAS_4xMmaPylT(UCA)_EF1_sfGFP150TGA

   Plasmid

   #174898

   Purpose

   opal suppression reporter sfGFP150TGA expression, with MmaPylT(UCA) opal suppressor tRNA cassette

   Depositor

   Simon Elsaesser

   Article

   Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

   Insert

   sfGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   150TGA in sfGFP

   Promoter

   EF1

   Available Since

   Nov. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFH2.99_PB-EPN1-MCP_I-T2A-eGFP-PGK-Blast

   Plasmid

   #205544

   Purpose

   Express EPN1-MCP-I-T2A-eGFP and blasticidin resistance marker

   Depositor

   Michael Elowitz

   Article

   Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

   Insert

   EPN1-MCP-I
   

   Use

   Piggybac

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   EF1

   Available Since

   Sept. 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFH2.98_PB-EPN1-MCP_Myc-T2A-eGFP-PGK-Blast

   Plasmid

   #205543

   Purpose

   Express EPN1-MCP-Myc-T2A-eGFP and blasticidin resistance marker

   Depositor

   Michael Elowitz

   Article

   Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

   Insert

   EPN1-MCP-Myc
   

   Use

   Piggybac

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   EF1

   Available Since

   Sept. 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pPIP1;4-NLS-3xmCitrine

   Plasmid

   #192524

   Purpose

   Fluorescent reporter for plant responses to bacterial infection

   Depositor

   Gitta Coaker

   Article

   Zhu et al Cell Rep. 2023 Jun 20;42(7):112676. doi:

   Insert

   PLASMA MEMBRANE INTRINSIC PROTEIN 1;4 (PIP1;4 Mustard Weed)
   

   Use

   Tags

   3xmCitrine and NLS

   Expression

   Plant

   Mutation

   Promoter

   PIP1;4

   Available Since

   June 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pACT2-μ1B (137-423)

    Plasmid

    #198172

    Purpose

    Expression of GAL4 transcriptional activation domain (AD)-AP-1 μ1B (137-423) fusion protein in yeast (yeast two-hybrid assays)

    Depositor

    Juan Bonifacino

    Article

    Guo et al Dev Cell. 2013 Nov 11;27(3):353-66. doi:

    Insert

    AP-1 μ1B (137-423)
    

    Use

    Tags

    GAL4 transcriptional activation domain (AD) fragm…

    Expression

    Yeast

    Mutation

    Promoter

    ADH1

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pXPR_003 sgNFkBIA guide 1

    Plasmid

    #193595

    Purpose

    NFkBIA knockout

    Depositor

    William Hahn

    Article

    Hagel et al Cancer Res. 2022 Dec 22:CAN-22-2245. doi

    Insert

    sgNFkBIA guide 1 (NFKBIA Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pXPR_003 sgNFkBIA guide 2

    Plasmid

    #193596

    Purpose

    NFkBIA knockout

    Depositor

    William Hahn

    Article

    Hagel et al Cancer Res. 2022 Dec 22:CAN-22-2245. doi

    Insert

    sgNFkBIA guide 2 (NFKBIA Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pXPR_003 sgNFkB2 guide 1

    Plasmid

    #193593

    Purpose

    NFkB2 knockout

    Depositor

    William Hahn

    Article

    Hagel et al Cancer Res. 2022 Dec 22:CAN-22-2245. doi

    Insert

    sgNFkB2 guide 1 (NFKB2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. attB53_SNCB-_attB53

    Plasmid

    #183615

    Purpose

    RMCE vector to shuttle puromycin resistance (+), anti-GFP SynNotch receptor (+), tagBFP (-) and TRE-mCherry (-) cassettes into attP50-flanked landing pad (Addgene 183609). (+) = sense (-) = antisense.

    Depositor

    Sally Lowell

    Article

    Malaguti et al Development. 2022 May 26. pii: 275525. d

    Inserts

    LaG17 GFP nanobody SynNotch tTA
    TRE-mCherry-SV40pA
    tagBFP
    

    Use

    Rmce

    Tags

    Myc and human CD8a signal sequence

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. GFP-Rock1-GBD (840-110 aa) in pEGFPN1

    Plasmid

    #187279

    Purpose

    Express EGFP-Rock1 GTPase-binding domain

    Depositor

    Alpha Yap

    Article

    Budnar et al Dev Cell. 2019 Jun 17;49(6):894-906.e12.

    Insert

    Rock1 (Rock1 Mouse)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    GBD (84-110aa)

    Promoter

    CMV

    Available Since

    Sept. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. Cherry-Plekhh1 5 silent mutations

    Plasmid

    #187373

    Purpose

    Expresses human Plekhh1 with 5 silent mutations labelled with Cherry

    Depositor

    Evelyne Coudrier

    Article

    Prosperi et al J Cell Sci. 2021 Dec 1;134(23). pii: 272

    Insert

    Plekhh1 with 5 silent mutations
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Five silent mutations at the Plekhh1 siRNA site (…

    Promoter

    CMV

    Available Since

    Aug. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. puc19_N_tag

    Plasmid

    #186650

    Purpose

    Empty donor plasmid.

    Depositor

    Astrid Haase

    Article

    Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

    Insert

    N-terminal 3xFlag-3xHA Tag
    

    Use

    Tags

    3xFlag-3xHA

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    July 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-CBA-Con/Fon-EYFP-WPRE

    Plasmid

    #186752

    Purpose

    AAV-mediated Cre and Flp-dependent expression of EYFP under CBA promoter

    Depositor

    Yongling Zhu

    Article

    Xu et al Cell Rep. 2022 Jul 5;40(1):111036. doi:

    Insert

    EYFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    CBA

    Available Since

    July 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pPOLY_new

    Plasmid

    #179276

    Purpose

    Expresses CuCBP, CcCDP and BaSP tricistronically in E. coli BL21(DE3)

    Depositor

    Bernd Nidetzky

    Article

    Schwaiger et al Microb Cell Fact. 2022 Apr 9;21(1):61. d

    Inserts

    cellodextrin phosphorylase
    cellobiose phosphorylase
    sucrose phosphorylase (C8077_RS00435 Bifidobacterium alodescentis)
    

    Use

    Synthetic Biology

    Tags

    6xHIS and Strep-Tag II

    Expression

    Bacterial

    Mutation

    Promoter

    T7 lacO

    Available Since

    April 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pT7-RiboJ-mTFP1-T7

    Plasmid

    #173225

    Purpose

    Encodes mTFP fluorescent protein. To be used in cell-free system

    Depositor

    Jim Haseloff

    Article

    Guzman-Chavez et al ACS Synth Biol. 2022 Mar 18;11(3):1114-1

    Insert

    mTFP1
    

    Use

    Cell-free system

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    March 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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