Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 8,591 results for: dykddddk

Showing: 6821 - 6840 of 8591 results

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SARS-CoV-2 spike G13 mutant

Plasmid

#231915

Purpose

For expression of SARS-CoV-2 spike protein with glycan deletion at N1098, N1158 by implementing Asn-to-Gln mutation

Depositor

Sriram Neelamegham

Article

Yang et al Npj Viruses. 2025 Jan 25;3(1):4. doi: 10

Insert

spike G13 (S SARS-CoV-2)

Tags

FLAG

Expression

Mammalian

Mutation

N1098Q, N1158Q

Promoter

CMV

Available Since

Jan. 27, 2025

Availability

Academic Institutions and Nonprofits only

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SARS-CoV-2 spike G14 mutant

Plasmid

#231916

Purpose

For expression of SARS-CoV-2 spike protein with glycan deletion at N1098, N1173 by implementing Asn-to-Gln mutation

Depositor

Sriram Neelamegham

Article

Yang et al Npj Viruses. 2025 Jan 25;3(1):4. doi: 10

Insert

spike G14 (S SARS-CoV-2)

Tags

FLAG

Expression

Mammalian

Mutation

N1098Q, N1173Q

Promoter

CMV

Available Since

Jan. 27, 2025

Availability

Academic Institutions and Nonprofits only

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SARS-CoV-2 spike G15 mutant

Plasmid

#231917

Purpose

For expression of SARS-CoV-2 spike protein with glycan deletion at N1098, N1194 by implementing Asn-to-Gln mutation

Depositor

Sriram Neelamegham

Article

Yang et al Npj Viruses. 2025 Jan 25;3(1):4. doi: 10

Insert

spike G15 (S SARS-CoV-2)

Tags

FLAG

Expression

Mammalian

Mutation

N1098Q, N1194Q

Promoter

CMV

Available Since

Jan. 27, 2025

Availability

Academic Institutions and Nonprofits only

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SARS-CoV-2 spike G145 mutant

Plasmid

#231918

Purpose

For expression of SARS-CoV-2 spike protein with glycan deletion at N1098, N1173, N1194 by implementing Asn-to-Gln mutation

Depositor

Sriram Neelamegham

Article

Yang et al Npj Viruses. 2025 Jan 25;3(1):4. doi: 10

Insert

spike G145 (S SARS-CoV-2)

Tags

FLAG

Expression

Mammalian

Mutation

N1098Q, N1173Q, N1194Q

Promoter

CMV

Available Since

Jan. 27, 2025

Availability

Academic Institutions and Nonprofits only

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SARS-CoV-2 spike G1345 mutant

Plasmid

#231919

Purpose

For expression of SARS-CoV-2 spike protein with glycan deletion at N1098, N1158, N1173, N1194 by implementing Asn-to-Gln mutation

Depositor

Sriram Neelamegham

Article

Yang et al Npj Viruses. 2025 Jan 25;3(1):4. doi: 10

Insert

spike G1345 (S SARS-CoV-2)

Tags

FLAG

Expression

Mammalian

Mutation

N1098Q, N1158Q, N1173Q, N1194Q

Promoter

CMV

Available Since

Jan. 27, 2025

Availability

Academic Institutions and Nonprofits only

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SARS-CoV-2 spike G1 mutant

Plasmid

#231913

Purpose

For expression of SARS-CoV-2 spike protein with glycan deletion at N1098 by implementing Asn-to-Gln mutation

Depositor

Sriram Neelamegham

Article

Yang et al Npj Viruses. 2025 Jan 25;3(1):4. doi: 10

Insert

spike G1 (S SARS-CoV-2)

Tags

FLAG

Expression

Mammalian

Mutation

N1098Q

Promoter

CMV

Available Since

Jan. 27, 2025

Availability

Academic Institutions and Nonprofits only

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SARS-CoV-2 spike G12 mutant

Plasmid

#231914

Purpose

For expression of SARS-CoV-2 spike protein with glycan deletion at N1098, N1134 by implementing Asn-to-Gln mutation

Depositor

Sriram Neelamegham

Article

Yang et al Npj Viruses. 2025 Jan 25;3(1):4. doi: 10

Insert

spike G12 (S SARS-CoV-2)

Tags

FLAG

Expression

Mammalian

Mutation

N1098Q, N1134Q

Promoter

CMV

Available Since

Jan. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pC2.4

Plasmid

#226557

Purpose

NATURA gene encoding plasmid

Depositor

Dave Wee

Article

Tabaglio et al Mol Ther Nucleic Acids. 2025 Jan 7;36(1)

Insert

NATURA reporter gene

Use

Luciferase; Piggybac

Tags

EGFP and Flag

Expression

Mammalian

Promoter

EF1alpha

Available Since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti-HA-CBS(opt)-FLAG

Plasmid

#230088

Purpose

expressing human CBS

Depositor

Xingguo Zhu

Article

Xi et al Commun Biol. 2025 Jan 6;8(1):15. doi: 10

Insert

CBS (CBS Human)

Use

Lentiviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

Multiple wobble mutations

Promoter

CMV

Available Since

Jan. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pET28b-SUMO-FLAG-TeKaiB-1-94-Y8A-Y94A-FLAG

Plasmid

#229223

Purpose

For expression of T. elongatus KaiB protein mutant

Depositor

Andy LiWang

Insert

kaiB (kaiB T. elongatus)

Tags

FLAG and SUMO-FLAG

Expression

Bacterial

Mutation

1-94 (truncated), Y8A, Y94A

Available Since

Jan. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pET28b-SUMO-FLAG-TeKaiB-FLAG

Plasmid

#229221

Purpose

For expression of T. elongatus KaiB protein

Depositor

Andy LiWang

Insert

kaiB (kaiB T. elongatus)

Tags

FLAG and SUMO-FLAG

Expression

Bacterial

Available Since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

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3xFLAG-DGKδ2

Plasmid

#226506

Purpose

Express N-terminal three tandem FLAG epitope tags, followed by an enterokinase cleavage site and human DGKD gene

Depositor

Chiaki Murakami, Fumio Sakane

Article

Murakami et al J Biol Chem. 2020 Mar 6;295(10):2932-294

Insert

DGKD (DGKD Human)

Tags

Three tandem FLAG epitope tag and enterokinase cl…

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

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3xFLAG-DGKδ2-ΔSAMD

Plasmid

#226507

Purpose

Express N-terminal three tandem FLAG epitope tags, followed by an enterokinase cleavage site and human DGKD gene deleted SAM domain

Depositor

Chiaki Murakami, Fumio Sakane

Article

Murakami et al J Biol Chem. 2020 Mar 6;295(10):2932-294

Insert

DGKD (DGKD Human)

Tags

Three tandem FLAG epitope tag and enterokinase cl…

Expression

Mammalian

Mutation

deleted sterile alpha motif domain (deleted amino…

Promoter

CMV

Available Since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

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FLAG-MKI-MUT-P2A-GFP

Plasmid

#229406

Purpose

Lentiviral or overpexression of cDNA

Depositor

Christopher Vakoc

Article

Klingbeil et al Cancer Discov. 2024 Jul 26. doi: 10.1158

Insert

MKI-MUT-P2A-GFP

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

interaction mutant L109G; L143G (Control mutant)

Promoter

EFS

Available Since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pET28b-SUMO-SeKaiB-K25C-FLAG

Plasmid

#229225

Purpose

For expression of S. elongatus KaiB protein mutant

Depositor

Andy LiWang

Insert

kaiB (kaiB S. elongatus)

Tags

FLAG and SUMO

Expression

Bacterial

Mutation

K25C

Available Since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

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CLIPf-KIF5A-SNAPf-HisTag-FLAG

Plasmid

#200789

Purpose

Mammalian expression of CLIPf-KIF5A-SNAPf-HisTag-FLAG

Depositor

Alison Twelvetrees

Article

Smith et al iScience. 2026 Feb 2;29(3):114875. doi:

Insert

KIF5A (Kif5a Mouse)

Tags

CLIPf and SNAPf, HisTag, FLAG

Expression

Mammalian

Promoter

CMV & T7

Available Since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

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CLIPf-KIF5A(Δ954-1027)-SNAPf-HisTag-FLAG

Plasmid

#200792

Purpose

Mammalian expression of CLIPf-KIF5A(Δ954-1027)-SNAPf-HisTag-FLAG

Depositor

Alison Twelvetrees

Article

Smith et al iScience. 2026 Feb 2;29(3):114875. doi:

Insert

KIF5A (Kif5a Mouse)

Tags

CLIPf and SNAPf, HisTag, FLAG

Expression

Mammalian

Mutation

Δ954-1027

Promoter

CMV & T7

Available Since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

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CLIPf-KIF5A(1-914; Δ505-610)-SNAPf-HisTag-FLAG

Plasmid

#200793

Purpose

Mammalian expression of CLIPf-KIF5A(1-914; Δ505-610)-SNAPf-HisTag-FLAG

Depositor

Alison Twelvetrees

Article

Smith et al iScience. 2026 Feb 2;29(3):114875. doi:

Insert

KIF5A (Kif5a Mouse)

Tags

CLIPf and SNAPf, HisTag, FLAG

Expression

Mammalian

Mutation

1-914; Δ505-610

Promoter

CMV & T7

Available Since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

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CLIPf-KIF5A(411-680)-SNAPf-HisTag-FLAG

Plasmid

#200798

Purpose

Mammalian expression of CLIPf-KIF5A(411-680)-SNAPf-HisTag-FLAG

Depositor

Alison Twelvetrees

Article

Smith et al iScience. 2026 Feb 2;29(3):114875. doi:

Insert

KIF5A (Kif5a Mouse)

Tags

CLIPf and SNAPf, HisTag, FLAG

Expression

Mammalian

Mutation

411-680

Promoter

CMV & T7

Available Since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

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FLAG-MARK3-GK-CR

Plasmid

#229424

Purpose

Lentiviral or overpexression of cDNA

Depositor

Christopher Vakoc

Article

Klingbeil et al Cancer Discov. 2024 Jul 26. doi: 10.1158

Insert

MARK3-GK-CR (MARK3 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

Gatekeeper mutation M132G; silent mutation CRISPR…

Promoter

EFS

Available Since

Dec. 19, 2024

Availability

Academic Institutions and Nonprofits only

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Showing: 6821 - 6840 of 8591 results

Results per page:

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