We narrowed to 9,085 results for: mel

1. pN1 Lck-GCaMP5G

   Plasmid

   #34924

   Purpose

   Expresses a fusion between the Lck domain and cytosolic GCaMP5G. This construct targets GCaMP5G to the plasma membrane

   Depositor

   Baljit Khakh

   Article

   Akerboom et al J Neurosci. 2012 Oct 3;32(40):13819-40.

   Insert

   Lck-GCaMP5G
   

   Expression

   Mammalian

   Mutation

   Plasmid contains N-terminal 26 amino acid membran…

   Promoter

   CMV

   Available Since

   Feb. 21, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCS2plus-TDP-43 M337V

   Plasmid

   #162609

   Purpose

   Allows for transcription of mutant TDP-43-M337V mRNA for injection into zebrafish embryos for BiFC assays

   Depositor

   Marco Morsch

   Article

   Don et al Mol Neurobiol. 2021 May;58(5):2061-2074.

   Insert

   TARDBP (TARDBP Human)
   

   Expression

   Mammalian

   Mutation

   M337V

   Available Since

   June 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCRISPaint-mScarlet-BlastR [M1G]

   Plasmid

   #171814

   Purpose

   Universal donor plasmid for CRISPitope method encoding mScarlet fluorescent protein and blasticidin resistance cassette [p.M1G]

   Depositor

   Michael Hoelzel

   Article

   Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.

   Insert

   mScarlet-T2A-BlastR
   

   Use

   Gene tagging

   Mutation

   BlastR: Changed Methionin 1 to Glycine.

   Available Since

   July 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pX330-TP53-1

   Plasmid

   #121917

   Purpose

   Encodes sgRNA targeting exon 4 of TP53

   Depositor

   William Hahn

   Article

   Howard et al Cancer Res. 2019 Feb 12. pii: 0008-5472.

   Insert

   TP53 (TP53 Human)
   

   Use

   CRISPR

   Promoter

   U6

   Available Since

   Feb. 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pX330-TP53-2

   Plasmid

   #121918

   Purpose

   Encodes sgRNA targeting exon 9 of TP53

   Depositor

   William Hahn

   Article

   Howard et al Cancer Res. 2019 Feb 12. pii: 0008-5472.

   Insert

   TP53 (TP53 Human)
   

   Use

   CRISPR

   Promoter

   U6

   Available Since

   Feb. 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. SmoM2 (W535L)-pcw107

   Plasmid

   #64627

   Purpose

   when used to produce lentivirus, express physiological levels of insert

   Depositor

   David Sabatini, Kris Wood

   Article

   Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

   Insert

   SMO (SMO Human)
   

   Use

   Lentiviral

   Mutation

   W535L

   Promoter

   PGK

   Available Since

   May 15, 2015

   Availability

   Academic Institutions and Nonprofits only

7. myr-FLAG-AKT1-pcw107

   Plasmid

   #64606

   Purpose

   when used to produce lentivirus, express physiological levels of insert

   Depositor

   David Sabatini, Kris Wood

   Article

   Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

   Insert

   AKT1 (AKT1 Human)
   

   Use

   Lentiviral

   Tags

   FLAG and myristoylated

   Promoter

   PGK

   Available Since

   May 12, 2015

   Availability

   Academic Institutions and Nonprofits only

8. Kras (G12V)-pcw107

   Plasmid

   #64602

   Purpose

   when used to produce lentivirus, express physiological levels of insert

   Depositor

   David Sabatini, Kris Wood

   Article

   Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

   Insert

   KRAS (KRAS Human)
   

   Use

   Lentiviral

   Mutation

   G12V

   Promoter

   PGK

   Available Since

   May 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. Notch1 intracellular domain-pcw107-V5

   Plasmid

   #64622

   Purpose

   when used to produce lentivirus, express physiological levels of insert

   Depositor

   David Sabatini, Kris Wood

   Article

   Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

   Insert

   NOTCH1 (NOTCH1 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Mutation

   intracellular domain *

   Promoter

   PGK

   Available Since

   Nov. 30, 2015

   Availability

   Academic Institutions and Nonprofits only

10. pLKO.1 Sox2 3H b

    Plasmid

    #26352

    Depositor

    Matthew Meyerson

    Article

    Bass et al Nat Genet. 2009 Nov . 41(11):1238-42.

    Insert

    Sox2 shRNA
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Available Since

    Sept. 22, 2010

    Availability

    Academic Institutions and Nonprofits only

11. pLVX-Phi3-AURKB-PGK-mCherry

    Plasmid

    #153316

    Purpose

    Expresses AURKB and mCherry in mammalian cells.

    Depositor

    Robert Judson-Torres

    Article

    McNeal et al Elife. 2021 Nov 23;10. pii: 70385. doi:

    Insert

    AURKB (AURKB Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. AR-V7-pcw107

    Plasmid

    #64635

    Purpose

    when used to produce lentivirus, express physiological levels of insert

    Depositor

    David Sabatini, Kris Wood

    Article

    Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

    Insert

    AR (transcript variant 1, splice isoform) (AR Human)
    

    Use

    Lentiviral

    Mutation

    splice isoform*

    Promoter

    PGK

    Available Since

    May 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. OCT4-GFP-2A-PURO transgenic reporter

    Plasmid

    #52379

    Purpose

    BAC recombineering generated construct, used as a reporter for OCT4 expression

    Depositor

    Jacob Hanna

    Article

    Gafni et al Nature. 2013 Dec 12;504(7479):282-6. doi

    Inserts

    GFP-2A-puro
    OCT4
    

    Expression

    Mammalian

    Promoter

    OCT4

    Available Since

    Oct. 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pET28a-AlkB

    Plasmid

    #228218

    Purpose

    Express wild type AlkB protein in E. coli BL21(DE3)

    Depositor

    Junchao Shi

    Article

    Shi et al Nat Cell Biol. 2021 Apr;23(4):424-436. d

    Insert

    Alpha-ketoglutarate-dependent Dioxygenase (alkB Synthetic)
    

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    codon optimized

    Promoter

    T7

    Available Since

    Jan. 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. beta-catenin (S33A, S37A, T41A, S45A)-pcw107

    Plasmid

    #64612

    Purpose

    when used to produce lentivirus, express physiological levels of insert

    Depositor

    David Sabatini, Kris Wood

    Article

    Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

    Insert

    Ctnnb1 (Ctnnb1 Mouse)
    

    Use

    Lentiviral

    Mutation

    S33A, S37A, T41A, S45A

    Promoter

    PGK

    Available Since

    May 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. beta-catenin (S33Y)-pcw107-V5

    Plasmid

    #64616

    Purpose

    when used to produce lentivirus, express physiological levels of insert

    Depositor

    David Sabatini, Kris Wood

    Article

    Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

    Insert

    CTNNB1 (CTNNB1 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Mutation

    S33Y

    Promoter

    PGK

    Available Since

    May 12, 2015

    Availability

    Academic Institutions and Nonprofits only

17. IKKalpha (S176E,S180E)-pcw107-V5

    Plasmid

    #64608

    Purpose

    when used to produce lentivirus, express physiological levels of insert

    Depositor

    David Sabatini, Kris Wood

    Article

    Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

    Insert

    CHUK (CHUK Human)
    

    Use

    Lentiviral

    Tags

    V5

    Mutation

    S176E, S180E

    Promoter

    PGK

    Available Since

    June 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pDONR221-GATA4

    Plasmid

    #101417

    Purpose

    Donor Vector containing GATA4 transcription factor, part of the Human TFome Collection

    Depositor

    George Church

    Article

    Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

    Insert

    GATA4 (GATA4 Human)
    

    Use

    Gateway shuttling vector

    Mutation

    Last nucleotide of stop codon removed to allow fo…

    Available Since

    June 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. NES-EGFP-PH-BTKx2

    Plasmid

    #183652

    Purpose

    PtdIns(3,4,5)P3 biosensor

    Depositor

    Sergio Grinstein

    Article

    Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

    Insert

    BTK (BTK Frog, Human)
    

    Tags

    X. laevis map2k1.L(32-44)-EGFP

    Expression

    Mammalian

    Mutation

    Amino acids 2-174 and 2-170

    Promoter

    CMV

    Available Since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCRISPaint-mNeon-PuroR [M1G]

    Plasmid

    #171800

    Purpose

    Universal donor plasmid for CRISPitope method encoding mNeon fluorescent protein and puromycin resistance cassette [p.M1G]

    Depositor

    Michael Hoelzel

    Article

    Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.

    Insert

    mNeonGreen-T2A-PuroR
    

    Use

    Gene tagging

    Mutation

    PuroR: Changed Methionin 1 to Glycine.

    Available Since

    Sept. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge