We narrowed to 7,068 results for: poly

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pHIV-EGFP.Flag-EZH2-mt2

Plasmid

#220245

Purpose

Expresses an EZH2 mutant for knockout/rescue experiments in mammalian cells.

Depositor

Chen Davidovich

Article

Gail et al Nat Genet. 2024 May 14. doi: 10.1038/s41

Insert

EZH2 mt 2 (EZH2 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

F32A, R34A, D36A, K39A, PRKKKR494-499NAAIRS

Promoter

EF-1α

Available Since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

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pSN840

Plasmid

#184832

Purpose

Expresses human KIF5A(∆exon27) and human KLC1 in insect cells

Depositor

Kyoko Chiba

Article

Nakano et al Genes Cells. 2022 Apr 17. doi: 10.1111/g

Inserts

KIF5A (∆exon27) (KIF5A Human)
KLC1 (KLC1 Human)

Use

Cre/Lox

Tags

His-FLAG and mScarlet-2xStrepII

Expression

Insect

Mutation

∆exon27 form of human KIF5A

Promoter

p10 and polyhedrin

Available Since

July 19, 2022

Availability

Academic Institutions and Nonprofits only

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pFB1.HMBP.PrS.RBBP4

Plasmid

#125164

Purpose

Expresses human RBBP4 in insect cells, , under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.

Depositor

Chen Davidovich

Article

Zhang et al Nat Struct Mol Biol. 2019 Mar;26(3):237-

Insert

Histone-binding protein RBBP4 (RBBP4 Human)

Tags

His-MBP

Expression

Insect

Promoter

Polyhedrin

Available Since

April 27, 2020

Availability

Academic Institutions and Nonprofits only

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pEW108

Plasmid

#232823

Purpose

Expresses yeast compass complex in insect cells

Depositor

Cynthia Wolberger

Article

Worden et al Elife. 2020 Jan 10;9. pii: 53199. doi: 1

Insert

COMPASS (Cps60, Cps50, Cps35, Cps25, Cps15, Flag-Set1, Cps30, Twinstrep-Cps40) (BRE2, SWD1, SET1, SPP1, SWD3, SWD2, SDC1, SHG1 Budding Yeast)

Tags

His6-3xFLAG, Twinstrep

Expression

Insect

Mutation

Set1(762-1080)

Promoter

polyhedrin promoter

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pFB1.HMBP.PrS.AEBP2

Plasmid

#125165

Purpose

Expresses human AEBP2 in insect cells, , under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.

Depositor

Chen Davidovich

Article

Zhang et al Nat Struct Mol Biol. 2019 Mar;26(3):237-

Insert

Zinc finger protein AEBP2 (AEBP2 Human)

Tags

His-MBP

Expression

Insect

Promoter

Polyhedrin

Available Since

April 27, 2020

Availability

Academic Institutions and Nonprofits only

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pVD1 tRNA-gRNA position E2 (GB2239)

Plasmid

#160561

Purpose

tRNA and scaffold for the assembly of GBoligomers for position [2-3] of a polycistronic tRNA-gRNA

Depositor

Diego Orzaez

Article

Vazquez-Vilar et al Front Plant Sci. 2021 Jul 1;12:689937. d

Insert

tRNA-gRNA position E2 (Multiplexing Edit)

Use

CRISPR and Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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pcDHa_mDDX5_FL_K144N

Plasmid

#88870

Purpose

constitutive expression of Ha-DDX5 K144N in mammalian cells

Depositor

Dan Littman

Article

Huang et al Nature. 2015 Dec 24;528(7583):517-22. do

Insert

DDX5 (Ddx5 Mouse)

Tags

HA

Expression

Mammalian

Mutation

K144N

Promoter

CMV

Available Since

June 12, 2017

Availability

Academic Institutions and Nonprofits only

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mCherry-CRY2-INPP5E

Plasmid

#79561

Purpose

expression of mCherry-tagged CRY2PHR fused to inositol 5-phosphatase domain of human INPP5E with mutated C-terminal CaaX-motif

Depositor

Pietro De Camilli, Olof Idevall-Hagren

Article

Idevall-Hagren et al Proc Natl Acad Sci U S A. 2012 Aug 28;10

Insert

INPP5E (INPP5E Human)

Tags

CRY2 (photolyase homology region domain of Arabid…

Expression

Mammalian

Mutation

C-terminal region, aa 214_644, C641A mutation to …

Promoter

CMV

Available Since

July 11, 2016

Availability

Academic Institutions and Nonprofits only
Donor_ANKRD1_KO_Puro

Plasmid

#186667

Purpose

Donor plasmid to endogenously knock out the ANKRD1 in Hek293 cells. eEF1a promoter, puromycin and polyA sequence is inserted between two homology arms.

Depositor

Astrid Haase

Article

Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

Insert

ANKRD1 KO Puromycin (ANKRD1 Human)

Expression

Mammalian

Available Since

Aug. 11, 2022

Availability

Academic Institutions and Nonprofits only

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Donor_YTHDF2_KO_Hygro

Plasmid

#186671

Purpose

Donor plasmid to endogenously knock out the YTHDF2 in Hek293 cells. Hygromycin and polyA sequence is inserted between two homology arms.

Depositor

Astrid Haase

Article

Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

Insert

YTHDF2 KO Hygromycin (YTHDF2 Human)

Expression

Mammalian

Available Since

Aug. 11, 2022

Availability

Academic Institutions and Nonprofits only

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Donor_YTHDF2_KO_Puro

Plasmid

#186670

Purpose

Donor plasmid to endogenously knock out the YTHDF2 in Hek293 cells. Puromycin and polyA sequence is inserted between two homology arms.

Depositor

Astrid Haase

Article

Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

Insert

YTHDF2 KO Puromycin (YTHDF2 Human)

Expression

Mammalian

Available Since

Aug. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pFl Strep-sumo-TEV-hAgo2 D597N

Plasmid

#136236

Purpose

Expressing catalytically inactive human Argonaute-2 in insect cells

Depositor

Leemor Joshua-Tor

Article

Elkayam et al Nucleic Acids Res. 2017 Apr 7;45(6):3528

Insert

human Argonaute-2 (AGO2 Human)

Tags

Sterp Sumo

Expression

Insect

Mutation

D597N

Promoter

polyhedrin

Available Since

April 21, 2020

Availability

Academic Institutions and Nonprofits only

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pHAGE2-TetOminiCMV-SM

Plasmid

#136578

Purpose

Dox-inducible polycistronic lentiviral vector expressing mouse Sox2, cMyc

Depositor

Hans Schöler

Article

Velychko et al Cell Stem Cell. 2019 Oct 30. pii: S1934-

Insert

Sox2-E2A-cMyc (Sox2, Myc Mouse)

Use

Lentiviral

Expression

Bacterial

Promoter

tetO-miniCMV (dox-inducible)

Available Since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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PTRF/Cavin1_L (OZ513)

Plasmid

#27188

Depositor

Keith Joung

Article

Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

Insert

Zinc finger array targeting PTRF/Cavin1 (cavin1b Zebrafish)

Use

Zebrafish targeting

Available Since

Feb. 8, 2011

Availability

Academic Institutions and Nonprofits only
pLIB_GST-nsp13 (SARS-CoV-2)

Plasmid

#169190

Purpose

Baculoviral transfer vector to express GST-nsp13 (SARS-CoV-2) in insect cells

Depositor

John Diffley

Article

Zeng et al Biochem J. 2021 Jul 16;478(13):2405-2423

Insert

GST-nsp13 (ORF1ab SARS-CoV-2, Synthetic)

Tags

GST

Expression

Insect

Mutation

Codon optimised for insect cells (Spodoptera frug…

Promoter

Polyhedrin

Available Since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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LIC4B-His-TEV-TAK1-TAB1

Plasmid

#154874

Purpose

Insect cell optimized TAK1-TAB1 construct used to solve structure and deposit PDB ID: 4O91

Depositor

Kenneth Westover

Article

Tan et al J Med Chem. 2015 Jan 8;58(1):183-96. doi

Insert

TAK1-TAB1 (TAB1 Human)

Tags

LIC TEV His

Expression

Insect

Mutation

Codon optimized

Promoter

polyhedrin

Available Since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pVD1 Multiplexing Edit En-1 (GB2242)

Plasmid

#160564

Purpose

tRNA and scaffold for the assembly of GBoligomers for the position [5_(n-1)] of a polycistronic tRNA-gRNA regulated by the U6-26 or U6-1 promoter.

Depositor

Diego Orzaez

Article

Vazquez-Vilar et al Front Plant Sci. 2021 Jul 1;12:689937. d

Insert

Multiplexing Edit (En-1)

Use

CRISPR and Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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pFBDMeGmTet1CD (pc2838)

Plasmid

#246924

Purpose

Expresses mCherry-tagged catalytic active domain of mouse Tet1 in mammalian cells. N-terminal tagged to GFP.

Depositor

Cristina Cardoso

Article

Ludwig et al Nucleic Acids Res. 2017 Mar 17;45(5):243

Insert

Tet1 (Tet1 Mouse)

Tags

GFP

Expression

Insect

Mutation

aa1365-2057

Promoter

Polyhedrin Promoter

Available Since

April 7, 2026

Availability

Academic Institutions and Nonprofits only

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pFBDMchmTet1CD (pc2859)

Plasmid

#246925

Purpose

Expresses mCherry-tagged catalytic active domain of mouse Tet1 in mammalian cells. N-terminal tagged to mCherry.

Depositor

Cristina Cardoso

Article

Ludwig et al Nucleic Acids Res. 2017 Mar 17;45(5):243

Insert

Tet1 (Tet1 Mouse)

Tags

mcherry

Expression

Insect

Mutation

aa 1365-2057

Promoter

Polyhedrin Promoter

Available Since

April 7, 2026

Availability

Academic Institutions and Nonprofits only

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pCAG1.1-human GALNTL5-3xHA

Plasmid

#241431

Purpose

The nucleotides coding human GALNTL5 variant #1 and 3xHA tag sequence were inserted under the CAG promoter.

Depositor

Taichi Noda

Article

Noda et al Nat Commun. 2025 Sep 17;16(1):8264. doi:

Insert

UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase-like 5 (GALNTL5 Human)

Tags

3xHA

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 9, 2025

Availability

Academic Institutions and Nonprofits only

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