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pCDEF3‐mTim1 (no tag)

Plasmid

#49206

Purpose

expresses mouse Tim1

Depositor

Lawrence Kane

Article

de Souza et al Proc Natl Acad Sci U S A. 2005 Nov 22;10

Insert

Tim1 (Havcr1 Mouse)

Expression

Mammalian

Promoter

EF1a

Available Since

Dec. 11, 2013

Availability

Academic Institutions and Nonprofits only
pJOE6089.4

Plasmid

#135081

Purpose

pBR322-based L-rhamnose-inducible vector.

Depositor

Josef Altenbuchner

Article

Hoffmann et al FEBS J. 2012 Oct;279(20):3911-24. doi: 1

Insert

P-rhaP-BAD-eGFP-Strep-tag II

Expression

Bacterial

Available Since

Feb. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV4-Flag-mIL-17RA/CFP.Hygro

Plasmid

#46861

Purpose

encodes Flag-tagged mouse IL-17RA fused to Cyan Fl. Protein

Depositor

Sarah Gaffen

Article

Maitra et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

IL-17RA-CFP

Tags

Flag/IL-3 signal sequence and fused to CFP

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 13, 2013

Availability

Academic Institutions and Nonprofits only
pPICZ:hTRAAK(G124I)

Plasmid

#130672

Purpose

P. pastoris expression vector. It will generate the human TRAAK channel (1-300) fused to a C-terminal GFP

Depositor

Dan Minor

Article

Lolicato et al Neuron. 2014 Dec 17;84(6):1198-212. doi:

Insert

KCNK4 (KCNK4 Human)

Tags

SNS- 3C_cleavage_site-TAAA-GFP

Expression

Yeast

Mutation

N104Q, N108Q, G124I

Available Since

May 15, 2020

Availability

Academic Institutions and Nonprofits only

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pNAS1b split mCherry NEDD4 WW2

Plasmid

#111882

Purpose

Mode #2 phosphosite expression (split mCherry, using NEDD4 WW2 binding domain)

Depositor

Jesse Rinehart

Article

Barber et al Nat Biotechnol. 2018 Aug;36(7):638-644.

Type

Empty backbone

Tags

Split mCherry system: NEDD4 WW2 fused to C-termin…

Expression

Bacterial

Available Since

July 20, 2018

Availability

Academic Institutions and Nonprofits only

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pBABE-puroGFP-Eps8WT

Plasmid

#74956

Purpose

mammalian expression of Eps8 fused to GFP

Depositor

Giorgio Scita

Article

Frittoli et al Immunity. 2011 Sep 23;35(3):388-99. doi:

Insert

Eps8 (Eps8 Mouse)

Tags

EGFP

Expression

Mammalian

Available Since

May 3, 2016

Availability

Academic Institutions and Nonprofits only
pCDNA3 Flag BimL(T56A,DE)

Plasmid

#25059

Depositor

Roger Davis

Article

Lei et al Proc Natl Acad Sci U S A. 2003 Mar 4. 10

Insert

BimL (T56A,DE) (BCL2L11 Human)

Tags

Flag

Expression

Mammalian

Mutation

Changed threonine 56 to alanine changed leucine …

Available Since

Aug. 13, 2010

Availability

Academic Institutions and Nonprofits only
pCDNA3 Flag BimL(T56D,DE)

Plasmid

#25060

Depositor

Roger Davis

Article

Lei et al Proc Natl Acad Sci U S A. 2003 Mar 4. 10

Insert

BimL (T56D,DE) (BCL2L11 Human)

Tags

Flag

Expression

Mammalian

Mutation

Changed threonine 56 to aspartic acid changed le…

Available Since

Aug. 13, 2010

Availability

Academic Institutions and Nonprofits only
KHBD00327

Plasmid

#34450

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG18783 (Kr-h1 Fly)

Use

Gateway donor vector

Available Since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only
3625 pSP72 Bim (genomic)

Plasmid

#8805

Depositor

Stanley Korsmeyer

Article

Harada et al Proc Natl Acad Sci U S A 2004 Oct 26;101

Insert

Bim genomic (Bcl2l11 Mouse)

Expression

Bacterial

Available Since

July 6, 2005

Availability

Academic Institutions and Nonprofits only
PAL-GFP

Plasmid

#221175

Purpose

Expresses the palmitolylation region of Arl13b and GFP-tagged

Depositor

Lei Lu

Article

Mahajan et al J Biol Chem. 2023 May;299(5):104604. doi

Insert

Arl13b (ARL13B )

Tags

EGFP

Expression

Mammalian

Mutation

deleted amino acids 10-428

Promoter

CMV promoter

Available Since

Jan. 9, 2026

Availability

Academic Institutions and Nonprofits only

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pETDuet::MGYFPDa829/MGYFPDa829I

Plasmid

#248877

Purpose

Expression of MGYFPDa829 and its immunity protein in bacterial cells for protein purification

Depositor

Brian Liau

Article

Roh et al Nat Methods. 2025 Oct;22(10):2083-2093.

Insert

6His-MGYFPDa829, MGYFPDa829I

Expression

Bacterial

Available Since

Dec. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pZNpF-∆11cag

Plasmid

#245370

Purpose

Dual luciferase (nano, firefly) with Zfp423 5' end fused to NanoLuciferase

Depositor

Bruce Hamilton

Article

Concepcion et al bioRxiv [Preprint]. 2025 Jun 2:2025.05.3

Insert

Zfp423-∆11cag (Zfp423 Mouse)

Use

Luciferase and Synthetic Biology

Tags

NanoLuciferase

Expression

Mammalian

Mutation

11-bp deletion, H96Wfs*4, plus 3 synonymous mutat…

Promoter

EF1a

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-EFS-DjCas13d-T2A-mCherry-pA

Plasmid

#192499

Purpose

To express DjCas13d

Depositor

Jonathan Ploski

Article

Back et al Mol Ther Methods Clin Dev. 2025 Aug 20;3

Insert

DjCas13d

Use

AAV

Mutation

No

Promoter

EFS

Available Since

Aug. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-0.5Syn-CasRX-mCherry-pA

Plasmid

#192491

Purpose

To express CasRx

Depositor

Jonathan Ploski

Article

Back et al Mol Ther Methods Clin Dev. 2025 Aug 20;3

Insert

CasRx

Use

AAV

Mutation

No

Promoter

0.5Syn

Available Since

Aug. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-EFS-CasRx-T2A-mCherry-pA

Plasmid

#192494

Purpose

To express CasRx

Depositor

Jonathan Ploski

Article

Back et al Mol Ther Methods Clin Dev. 2025 Aug 20;3

Insert

CasRx

Use

AAV

Mutation

No

Promoter

EFS

Available Since

Aug. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-intron-DjCas13d-pA

Plasmid

#192495

Purpose

To express DjCas13d

Depositor

Jonathan Ploski

Article

Back et al Mol Ther Methods Clin Dev. 2025 Aug 20;3

Insert

DjCas13d

Use

AAV

Mutation

No

Promoter

CMV

Available Since

Aug. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-intron-DIO-DjCas13d-pA

Plasmid

#192498

Purpose

To express DjCas13d in a Cre dependent manner

Depositor

Jonathan Ploski

Article

Back et al Mol Ther Methods Clin Dev. 2025 Aug 20;3

Insert

DjCas13d

Use

AAV

Mutation

No

Promoter

CMV

Available Since

Aug. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-EFS-CasRx-pA

Plasmid

#192482

Purpose

To express CasRx

Depositor

Jonathan Ploski

Article

Back et al Mol Ther Methods Clin Dev. 2025 Aug 20;3

Insert

CasRx

Use

AAV

Mutation

No

Promoter

EFS

Available Since

Aug. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-0.4CaMKII-CasRx-T2A-mCherry-pA

Plasmid

#192483

Purpose

To express CasRx

Depositor

Jonathan Ploski

Article

Back et al Mol Ther Methods Clin Dev. 2025 Aug 20;3

Insert

CasRx

Use

AAV

Mutation

No

Promoter

0.4CaMKIIa

Available Since

Aug. 27, 2025

Availability

Academic Institutions and Nonprofits only

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