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We narrowed to 13,841 results for: Cre

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Showing: 6841 - 6860 of 13841 results

  2. StrKDEL-IRES-mannosidase II-mTagBFP2

    Plasmid
    #165460
    Purpose
    Expressed mannII-BFP simultaneously with a RUSH Hook Str-KDEL.
    Depositor
    David Stephens
    Publication
    McCaughey et al J Cell Sci. 2021 Aug 5. pii: 271201. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Partial ManII-mTag2BFP fusion (Man2a1 Mouse)
    Tags
    Str-KDEL and mTag2BFP
    Expression
    Mammalian
    Mutation
    contains only amino acids 1 to 116 of ManII
    Promoter
    CMV
    Available since
    Aug. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    165460-plasmid-map-sequence-id-331274

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  3. pCC_12 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-KRAB-dxCas9NG-NLS-2A-Puro-WPRE

    Plasmid
    #139097
    Purpose
    Expresses human codon-optimized inactive xCas9-NG fused to a transcriptional repressor KRAB in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a barcode downstream of sgRNA cassette.
    Depositor
    Neville Sanjana
    Publication
    Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.
    Insert
    KRAB-dxCas9-NG
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Mutation
    D10A, H840A, A262T,R324L, S409I, E480K, E543D, M6…
    Available since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    139097-plasmid-map-sequence-id-273896

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  7. TFORF1879

    Plasmid
    #141880
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Publication
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    CREBZF (CREBZF Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    141880-plasmid-map-sequence-id-415057

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  11. HCTR1-DuET

    Plasmid
    #213310
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Publication
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    HCTR1 (HCRTR1 Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213310-plasmid-map-sequence-id-430848

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  12. pFN35K SmBiT TK-neo Human collagen type IV alpha 3 chain (COL4A3)

    Plasmid
    #229767
    Purpose
    Human Col4A3 tagged at N-terminal with SmBiT to be used with N-tagged LgBiT hCol4A5 and Flag-tagged hCol4A4 in Nanobit assay system
    Depositor
    Hirofumi Kai
    Publication
    Omachi et al Cell Chem Biol. 2018 May 17;25(5):634-64
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Human collagen type IV alpha 3 chain (COL4A3) (COL4A3 Human)
    Use
    Luciferase
    Tags
    SmBiT
    Mutation
    See Depositor Comments
    Available since
    Feb. 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    229767-plasmid-map-sequence-id-457423

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  13. pBIG2abc_nsp12-His6-3xFlag/nsp7/nsp8 (SARS-CoV-2)

    Plasmid
    #169185
    Purpose
    Baculoviral transfer vector to co-express nsp12-His6-3xFlag, nsp7 and nsp8 (SARS-CoV-2) in insect cells
    Depositor
    John Diffley
    Publication
    Bertolin et al Biochem J. 2021 Jul 16;478(13):2425-2443
    Insert
    nsp12-His6-3xFlag/nsp7/nsp8 (ORF1ab Synthetic, SARS-CoV-2)
    Tags
    His6-3xFlag (nsp12 C-terminus)
    Expression
    Insect
    Mutation
    Codon optimised for insect cells (Spodoptera frug…
    Promoter
    Polyhedrin
    Available since
    July 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    169185-plasmid-map-sequence-id-334217

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  14. pGL2 HNF4A P2 mut

    Plasmid
    #60325
    Purpose
    Mutated HNF4A P2 promoter region cloned into the pGL2-basic backbone. The -181 G to A mutation is in a HNF1alpha binding site and cosegregates with MODY.
    Depositor
    Jorge Ferrer
    Publication
    Hansen et al J Clin Invest. 2002 Sep;110(6):827-33.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    HNF4A P2 mut (-181 G to A) (HNF4A Human)
    Use
    Luciferase
    Tags
    Luciferase
    Expression
    Mammalian
    Mutation
    The -181 G to A mutation is in a HNF1alpha bindin…
    Available since
    Jan. 6, 2015
    Availability
    Academic Institutions and Nonprofits only

  20. pVH613

    Plasmid
    #169602
    Purpose
    Tier-2 vector encoding PCREm4-driven Nluc and PmPGK1-driven Fluc expression (PCREm4-Nluc-pA::A2-pA::PmPGK1-Fluc-pA).
    Depositor
    Martin Fussenegger
    Publication
    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    cAMP response element-controlled NanoLuc Luciferase expression cassette
    Expression
    Mammalian
    Promoter
    PCREm4 / PPGK
    Available since
    June 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    169602-plasmid-map-sequence-id-365597

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Showing: 6841 - 6860 of 13841 results