We narrowed to 15,169 results for: invs

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KP501: pMVP (L5-L4) eYFP-P2A

Plasmid

#121714

Purpose

pMVP L5-L4 entry plasmid, contains eYFP-P2A for 4-component MultiSite Gateway Pro assembly. Allows expression of N-term eYFP linked by P2A to gene of interest.

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

eYFP-P2A (open)

Use

Synthetic Biology; Pmvp gateway entry plasmid

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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HA-MBP-TEVpCS-ADGRE4 CTFdeltaTA-FLAG

Plasmid

#217725

Purpose

Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

Depositor

Stephen Blacklow

Article

Dates et al Cell Chem Biol. 2024 Aug 15;31(8):1542-1

Insert

ADGRE4

Tags

FLAG and HA

Expression

Mammalian

Mutation

Deletion of residues 1-179

Available Since

April 16, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3/Myc-DNMT3B3

Plasmid

#37546

Depositor

Arthur Riggs

Article

Chen et al J Cell Biochem. 2005 Aug 1;95(5):902-17.

Insert

DNMT3B3 (DNMT3B Human)

Tags

Myc

Expression

Mammalian

Available Since

Aug. 15, 2012

Availability

Academic Institutions and Nonprofits only

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Yellow Cameleon-Nano140/pcDNA3

Plasmid

#51966

Purpose

mammalian expression of Yellow Cameleon-Nano140, an ultrasensitive Ca2+ indicator with Kd = 140 nM

Depositor

Takeharu Nagai

Article

Horikawa et al Nat Methods. 2010 Sep;7(9):729-32. doi:

Insert

Yellow Cameleon-Nano140

Expression

Mammalian

Available Since

March 18, 2014

Availability

Academic Institutions and Nonprofits only

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pCEP4-ZM197M-gp160

Plasmid

#123226

Purpose

Mammalian expression plasmid for Env from the ZM197M HIV-1 isolate

Depositor

Erik Procko

Article

Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

Insert

HIV-1 (ZM197M) Env

Tags

CD5 leader peptide

Expression

Mammalian

Mutation

Codon-optimized synthetic gene

Promoter

CMV

Available Since

April 2, 2019

Availability

Academic Institutions and Nonprofits only

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pCDNA3-Myc3-Cul3 delta N41

Plasmid

#21590

Depositor

Yue Xiong

Article

Furukawa et al Mol Cell Biol. 2005 Jan . 25(1):162-71.

Insert

cullin 3 (CUL3 Human)

Tags

myc3

Expression

Mammalian

Mutation

Residues 34-74 deleted from N terminus.

Available Since

July 1, 2009

Availability

Academic Institutions and Nonprofits only
pcDNA3-sapphireCKAR(T/A)

Plasmid

#118469

Purpose

Negative-control mutant for sapphireCKAR biosensor.

Depositor

Jin Zhang

Article

Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

Insert

sapphireCKAR(T/A)

Tags

6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…

Expression

Mammalian

Mutation

Contains Thr-to-Ala mutation in substrate sequenc…

Promoter

CMV

Available Since

Dec. 6, 2018

Availability

Academic Institutions and Nonprofits only

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LacZ-5BoxB in pcDNA3.1+

Plasmid

#29729

Depositor

Howard Chang

Article

Wang et al Nature. 2011 Apr 7;472(7341):120-4. doi:

Insert

LacZ-5BoxB

Expression

Mammalian

Available Since

May 23, 2011

Availability

Academic Institutions and Nonprofits only
3492 pcDNA3 Bad

Plasmid

#8778

Depositor

Stanley Korsmeyer

Article

Harada et al Proc Natl Acad Sci U S A 2001 Aug 14;98(

Insert

Bad (Bad Mouse)

Expression

Mammalian

Available Since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only
pcDNA5/FRT/TO DNAJB5

Plasmid

#19473

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

Insert

DNAJB5 (DNAJB5 Human)

Expression

Mammalian

Available Since

Oct. 6, 2008

Availability

Academic Institutions and Nonprofits only

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PM-ABKAR

Plasmid

#61506

Purpose

Expression of AMPK biosensor targeted to the plasma membrane via Lyn kinase targeting sequence

Depositor

Takanari Inoue, Jin Zhang

Article

Miyamoto et al Cell Rep. 2015 Apr 28;11(4):657-70. doi:

Insert

AMPK Biosensor

Tags

Lyn kinase sequence (see comments)

Expression

Mammalian

Promoter

CMV

Available Since

April 17, 2015

Availability

Academic Institutions and Nonprofits only
hHSF1 K80R

Plasmid

#118350

Purpose

Plasmid expresses hHSF1 with K80 in DBD mutated to R.

Depositor

Lea Sistonen

Article

Westerheide et al Science. 2009 Feb 20. 323(5917):1063-6.

Insert

hHSF1 (HSF1 Human)

Tags

myc and 6xHIS

Expression

Mammalian

Mutation

K80R

Available Since

Nov. 8, 2018

Availability

Academic Institutions and Nonprofits only

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VinTS-tCRMod-GGSGGS7

Plasmid

#111764

Purpose

Expresses vinculin tension sensor comprised of Clover-(GGSGGS)7-mRuby2 module

Depositor

Brenton Hoffman

Article

LaCroix et al eLife. 2018 Jul 19;7. pii: 33927. doi: 1

Insert

Clover-mRuby2-based vinculin tension sensor with (GGSGGS)7 extensible domain

Expression

Mammalian

Mutation

Residues 1-228 of Clover, residues 1-3 deleted fr…

Promoter

CMV

Available Since

July 5, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-RAB-EKARev(T/A)

Plasmid

#118475

Purpose

Negative-control mutant for RAB-EKARev biosensor.

Depositor

Jin Zhang

Article

Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

Insert

RAB-EKARev(T/A)

Tags

6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…

Expression

Mammalian

Mutation

Contains Thr-to-Ala mutation in substrate sequenc…

Promoter

CMV

Available Since

May 21, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3/Myc-DNMT3B2

Plasmid

#36942

Depositor

Arthur Riggs

Article

Chen et al J Cell Biochem. 2005 Aug 1;95(5):902-17.

Insert

DNMT3B2 (DNMT3B Human)

Tags

Myc

Expression

Mammalian

Available Since

June 22, 2012

Availability

Academic Institutions and Nonprofits only
YFP Gamma 3

Plasmid

#36103

Depositor

Narasimhan Gautam

Article

Saini et al J Biol Chem. 2007 Aug 17;282(33):24099-1

Insert

G protein subunit Gamma 3 (Gng3 Mouse)

Tags

YFP

Expression

Mammalian

Promoter

CMV

Available Since

May 3, 2012

Availability

Academic Institutions and Nonprofits only
pcDNA3/mArf1(T31N)-HA

Plasmid

#79411

Purpose

Expresses C-terminally HA-tagged Arf1(T31N) in mammalian cells

Depositor

Kazuhisa Nakayama

Article

Kondo et al Cell Struct Funct. 2012;37(2):141-54. Ep

Insert

Arf1 (Arf1 Mouse)

Tags

HA

Expression

Mammalian

Mutation

changed Threonine 31 to Asparagine

Available Since

April 25, 2017

Availability

Academic Institutions and Nonprofits only

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pcFLAG RUNX3 (R122C)

Plasmid

#203438

Purpose

Mammalian expression of RUNX3

Depositor

Yoshiaki Ito

Article

Oei et al Commun Biol. 2023 Jul 3;6(1):689. doi: 1

Insert

RUNX3 (RUNX3 Human)

Tags

FLAG

Expression

Mammalian

Available Since

Aug. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-ER-CaNAR2

Plasmid

#64732

Purpose

Endoplasmic reticulum (ER)-targeted FRET biosensor for monitoring calcineurin (CaN) phosphatase activity; targeted to cytosolic face of ER membrane.

Depositor

Jin Zhang

Article

Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.755

Insert

CYP450-CaNAR2

Tags

Cerulean3, N-terminal 27 amino acids from CYP450,…

Expression

Mammalian

Promoter

CMV

Available Since

July 21, 2015

Availability

Academic Institutions and Nonprofits only

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pFastBac1 hBRM

Plasmid

#1956

Depositor

Robert Kingston

Article

Phelan et al Mol Cell 1999 Feb;3(2):247-53.

Insert

BRM (SMARCA2 Human)

Tags

flag

Expression

Insect

Available Since

May 24, 2006

Availability

Academic Institutions and Nonprofits only