We narrowed to 9,085 results for: mel

1. pAAV-EF1α1.1-ChrimsonR-GFP

   Plasmid

   #122063

   Purpose

   AAV-mediated expression of ChrimsonR-GFP under the EF1α promoter (1.1kb short version). Using SV40 pA signal.

   Depositor

   Edward Boyden

   Article

   Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

   Has Service

   AAV2

   Insert

   ChrimsonR-GFP
   

   Use

   AAV

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Chrimson K176R mutant

   Promoter

   EF1a（1.1kb short version)

   Available Since

   March 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. AR-V7-pcw107-V5

   Plasmid

   #64636

   Purpose

   when used to produce lentivirus, express physiological levels of insert

   Depositor

   David Sabatini, Kris Wood

   Article

   Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

   Insert

   AR (transcript variant 1, splice isoform) (AR Human)
   

   Use

   Lentiviral

   Tags

   V5

   Mutation

   splice isoform *

   Promoter

   PGK

   Available Since

   Dec. 1, 2015

   Availability

   Academic Institutions and Nonprofits only

3. pGL3Basic_ME.1/ApoEpromoter

   Plasmid

   #51435

   Purpose

   This plasmid is a luciferase-based reporter construct to measure the activity of the ApoE promoter fused to ME.1 enhancer

   Depositor

   Sohail Tavazoie

   Article

   Pencheva et al Cell. 2014 Feb 27;156(5):986-1001. doi:

   Insert

   ME.1+ApoE promoter -980/+(93 bp.) (APOE, ME1 Human)
   

   Use

   Luciferase

   Expression

   Mammalian

   Mutation

   ME.1 enhancer is fused upstream of ApoE promoter …

   Available Since

   Feb. 27, 2014

   Availability

   Academic Institutions and Nonprofits only

4. pNTK human MBD3 PGK-Neo-pA flox

   Plasmid

   #52362

   Purpose

   Targeting vector for Human MBD3 conditional knockout. Also creates hypomorphic alleles.

   Depositor

   Jacob Hanna

   Article

   Rais et al Nature. 2013 Oct 3;502(7469):65-70. doi:

   Insert

   MBD3 (MBD3 Human)
   

   Use

   Targeting

   Available Since

   Sept. 8, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. NES-mCherry-PH-BTKx2

   Plasmid

   #183654

   Purpose

   PtdIns(3,4,5)P3 biosensor

   Depositor

   Sergio Grinstein

   Article

   Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

   Insert

   BTK (BTK Frog, Human)
   

   Tags

   X. laevis map2k1.L(32-44)-mCherry

   Expression

   Mammalian

   Mutation

   Amino acids 2-174 and 2-170

   Promoter

   CMV

   Available Since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. Notch1 intracellular domain-pcw107

   Plasmid

   #64621

   Purpose

   when used to produce lentivirus, express physiological levels of insert

   Depositor

   David Sabatini, Kris Wood

   Article

   Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

   Insert

   NOTCH1 (NOTCH1 Human)
   

   Use

   Lentiviral

   Mutation

   intracellular domain *

   Promoter

   PGK

   Available Since

   May 12, 2015

   Availability

   Academic Institutions and Nonprofits only

7. pCRISPaint-mNeon-BlastR [M1G]

   Plasmid

   #171805

   Purpose

   Universal donor plasmid for CRISPitope method encoding mNeon fluorescent protein and blasticidin resistance cassette [p.M1G]

   Depositor

   Michael Hoelzel

   Article

   Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.

   Insert

   mNeonGreen-T2A-BlastR
   

   Use

   Gene tagging

   Mutation

   BlastR: Changed Methionin 1 to Glycine.

   Available Since

   July 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. TMEM230-WT-DsRed(isoform 2)

   Plasmid

   #99563

   Purpose

   mammalian expression of TMEM230-dsRed (isoform 2)

   Depositor

   Han-Xiang Deng

   Article

   Deng et al Nat Genet. 2016 Jul;48(7):733-9. doi: 10

   Insert

   TMEM230 (TMEM230 Human)
   

   Tags

   DsRed

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 16, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-Syn-FLEX-fd [ChrimsonR-tdTomato]

   Plasmid

   #84483

   Purpose

   AAV-mediated expression of ChrimsonR-tdTomato under the Syn promoter, in floxed/forward (Cre-dependent) manner. Cre turns gene off. Using bGHpA signal. tdTomato is a codon diversified version.

   Depositor

   Edward Boyden

   Article

   Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

   Insert

   ChrimsonR-tdTomato
   

   Use

   AAV

   Tags

   tdTomato

   Expression

   Mammalian

   Mutation

   Chrimson K176R mutant

   Promoter

   human synapsin promoter

   Available Since

   April 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. Hras (G12V)-pcw107

    Plasmid

    #64603

    Purpose

    when used to produce lentivirus, express physiological levels of insert

    Depositor

    David Sabatini, Kris Wood

    Article

    Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

    Insert

    HRAS (HRAS Human)
    

    Use

    Lentiviral

    Mutation

    G12V

    Promoter

    PGK

    Available Since

    May 12, 2015

    Availability

    Academic Institutions and Nonprofits only

11. AAV-Syn-FLPX-rc [ChrimsonR-tdTomato]

    Plasmid

    #128589

    Purpose

    AAV-mediated expression of ChrimsonR-tdTomato under the Syn promoter in frt/reversed (Flp-dependent) manner. tdTomato has codons varied to reduce recombination.

    Depositor

    Edward Boyden

    Article

    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

    Has Service

    AAV8

    Insert

    ChrimsonR-tdTomato
    

    Use

    AAV

    Tags

    tdTomato (codon diversified version)

    Expression

    Mammalian

    Mutation

    Chrimson K176R mutant

    Promoter

    Syn

    Available Since

    Sept. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. TRCN0000338462

    Plasmid

    #78158

    Purpose

    shXPO1 targeting CDS

    Depositor

    William Hahn

    Article

    Hong et al Nat Commun. 2016 Jun 22;7:11987. doi: 10

    Insert

    XPO1 (XPO1 Human)
    

    Use

    Lentiviral and RNAi

    Promoter

    hU6 and hU6

    Available Since

    June 22, 2016

    Availability

    Academic Institutions and Nonprofits only

13. pDONR221-FOXN1

    Plasmid

    #101445

    Purpose

    Donor Vector containing FOXN1 transcription factor, part of the Human TFome Collection

    Depositor

    George Church

    Article

    Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

    Insert

    FOXN1 (FOXN1 Human)
    

    Use

    Gateway shuttling vector

    Mutation

    Last nucleotide of stop codon removed to allow fo…

    Available Since

    June 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. MEK5 DD(S311D,T315D)-pcw107-V5

    Plasmid

    #64619

    Purpose

    when used to produce lentivirus, express physiological levels of insert

    Depositor

    David Sabatini, Kris Wood

    Article

    Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

    Insert

    MAP2K5 (MAP2K5 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Mutation

    S311D, T315D*

    Promoter

    PGK

    Available Since

    June 1, 2015

    Availability

    Academic Institutions and Nonprofits only

15. p38 WT O/E (MAPK14)-pcw107-V5

    Plasmid

    #64624

    Purpose

    when used to produce lentivirus, express physiological levels of insert

    Depositor

    David Sabatini, Kris Wood

    Article

    Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

    Insert

    MAPK14 (transcript variant 2) (MAPK14 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Promoter

    PGK

    Available Since

    May 12, 2015

    Availability

    Academic Institutions and Nonprofits only

16. pCS2plus-mCerulean 156-239-GGGS-TDP-43 M337V

    Plasmid

    #162618

    Purpose

    Allows for transcription of mCerulean fluorophore half fused to mutant TDP-43-M337V for injection into zebrafish embryos for BiFC assays

    Depositor

    Marco Morsch

    Article

    Don et al Mol Neurobiol. 2021 May;58(5):2061-2074.

    Insert

    TARDBP (TARDBP Human)
    

    Expression

    Mammalian

    Mutation

    M337V

    Available Since

    June 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. AAV-EF1α1.1-FLPX-rc [ChrimsonR-tdTomato]

    Plasmid

    #128587

    Purpose

    AAV-mediated expression of ChrimsonR-tdTomato under the EF1α1.1 promoter in frt/reversed (Flp-dependent) manner. tdTomato has codons varied to reduce recombination.

    Depositor

    Edward Boyden

    Article

    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

    Insert

    ChrimsonR-tdTomato
    

    Use

    AAV

    Tags

    tdTomato (codon diversified version)

    Expression

    Mammalian

    Mutation

    Chrimson K176R mutant

    Promoter

    EF1α (1.1 kb short version)

    Available Since

    Sept. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. FUW-Flag-Mbd3 delta1-70

    Plasmid

    #52372

    Purpose

    Lentiviral expression vector for constitutive expression of double Flag tagged Mbd3 mutant

    Depositor

    Jacob Hanna

    Article

    Rais et al Nature. 2013 Oct 3;502(7469):65-70. doi:

    Insert

    Mbd3 (Mbd3 Mouse)
    

    Use

    Lentiviral

    Tags

    flag

    Expression

    Mammalian

    Mutation

    deleted amino acids 1-70

    Promoter

    Ubiquitin

    Available Since

    Sept. 8, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. FUW-Flag-Mbd3 delta 25-83

    Plasmid

    #52373

    Purpose

    Lentiviral expression vector for constitutive expression of double Flag tagged Mbd3 mutant

    Depositor

    Jacob Hanna

    Article

    Rais et al Nature. 2013 Oct 3;502(7469):65-70. doi:

    Insert

    Mbd3 (Mbd3 Mouse)
    

    Use

    Lentiviral

    Tags

    flag

    Expression

    Mammalian

    Mutation

    amino acids 25-83 deleted

    Promoter

    Ubiquitin

    Available Since

    Sept. 8, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. FUW-Flag-Mbd3 delta49-63

    Plasmid

    #52374

    Purpose

    Lentiviral expression vector for constitutive expression of double Flag tagged Mbd3 mutant

    Depositor

    Jacob Hanna

    Article

    Rais et al Nature. 2013 Oct 3;502(7469):65-70. doi:

    Insert

    Mbd3 (Mbd3 Mouse)
    

    Use

    Lentiviral

    Tags

    flag

    Expression

    Mammalian

    Mutation

    deleted amino acids 49-63

    Promoter

    Ubiquitin

    Available Since

    Sept. 8, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge