We narrowed to 10,396 results for: plasmids 101

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pAAV-FLEXfrt-SaCas9-U6-sgGabrg3

Plasmid

#124872

Purpose

Mutagenesis of Gabrg3

Depositor

Larry Zweifel

Article

Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

Insert

Gabrg3 (Gabrg3 Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Available Since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-FLEXfrt-SaCas9-U6-sgGabrg2

Plasmid

#124871

Purpose

Mutagenesis of Gabrg2

Depositor

Larry Zweifel

Article

Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

Insert

Gabrg2 (Gabrg2 Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Available Since

May 3, 2019

Availability

Academic Institutions and Nonprofits only

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pU6-1 (GB1204)

Plasmid

#75405

Purpose

Provides the A. thaliana U6-1 RNA polIII promoter as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Vazquez-Vilar et al Plant Methods. 2016 Feb 1;12:10. doi: 10

Insert

AtU6-1 promoter

Use

CRISPR and Synthetic Biology

Expression

Plant

Available Since

Aug. 5, 2016

Availability

Academic Institutions and Nonprofits only

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gh6

Plasmid

#106690

Purpose

expression of gRNA targeting GALNT6

Depositor

Eric-Paul Bennett

Article

Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

Insert

GALNT6 (GALNT6 Human)

Expression

Mammalian

Available Since

April 26, 2019

Availability

Academic Institutions and Nonprofits only

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pAG415-GPDp-EGFP-rap1-K651R

Plasmid

#84536

Purpose

This plasmid can be used to express EGFP-tagged Rap1-K651R from the GDP promoter in yeast

Depositor

Jorrit Enserink

Article

Chymkowitch et al Genome Res. 2015 Jun;25(6):897-906. doi:

Insert

RAP1 (RAP1 Budding Yeast)

Tags

EGFP

Expression

Yeast

Mutation

K651R

Promoter

GPD

Available Since

Dec. 22, 2016

Availability

Academic Institutions and Nonprofits only
pZNpF-∆48∆11

Plasmid

#245369

Purpose

Dual luciferase (nano, firefly) with Zfp423 5' end fused to NanoLuciferase

Depositor

Bruce Hamilton

Article

Concepcion et al bioRxiv [Preprint]. 2025 Jun 2:2025.05.3

Insert

Zfp423-∆48∆11 (Zfp423 Mouse)

Use

Luciferase and Synthetic Biology

Tags

NanoLuciferase

Expression

Mammalian

Mutation

11-bp deletion, H96Wfs*4, plus synthetic 48-bp up…

Promoter

EF1a

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pCSIIpuro-TGFa-mNeonGreen

Plasmid

#209913

Purpose

To monitor the expression of TGFα, the plasmid encodes a recombinant TGFα fused intracellularly to the mNeonGreen fluorescent protein

Depositor

Michiyuki Matsuda

Article

Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi

Insert

TGFa-mNeonGreen (TGFA Human)

Use

Lentiviral

Tags

mNeonGreen

Available Since

Nov. 18, 2024

Availability

Academic Institutions and Nonprofits only

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FgH1tUTG-sgRNA.PRDM1-ZEB2_CRISPRd

Plasmid

#216171

Purpose

Express the gRNA targeting the PRDM1-binding site in the human ZEB2 locus for the CRISPRd experiment

Depositor

Makiko Iwafuchi

Article

Matsui et al Mol Cell. 2024 Jan 4:S1097-2765(23)01026

Insert

sgRNA- PRDM1.bs- ZEB2.locus (PRDM1 Human)

Use

Lentiviral

Tags

EGFP

Available Since

July 29, 2024

Availability

Academic Institutions and Nonprofits only

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pOTTC1642 - pAAV EF1a EGFP-KASH with mTACR1 gRNAs

Plasmid

#195018

Purpose

An adeno-associated viral vector expressing eGFP-KASH and two guides targeting mouse TACR1

Depositor

Christopher Richie

Article

Belilos et al bioRxiv [Preprint]. 2023 Oct 9:2023.02.0

Inserts

CCGTATAGGCGGCTGCCCAA
EGFP-KASH
TTCCGTGGTGGGCAACGTAG

Use

AAV

Tags

KASH domain of Nesprin2

Promoter

EF1a, hU6, and mU6

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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Dyn1 R271E

Plasmid

#198343

Purpose

Mammalian expression plasmid of GFP-chimera proteins.

Depositor

Justin Taraska

Article

Jimah et al Dev Cell. 2024 Apr 23:S1534-5807(24)0023

Insert

Dynamin1 (DNM1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

R271E

Promoter

CMV

Available Since

April 24, 2024

Availability

Academic Institutions and Nonprofits only

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Dyn1 K44A

Plasmid

#198340

Purpose

Mammalian expression plasmid of GFP-chimera proteins.

Depositor

Justin Taraska

Article

Jimah et al Dev Cell. 2024 Apr 23:S1534-5807(24)0023

Insert

Dynamin1 (DNM1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

K44A

Promoter

CMV

Available Since

April 24, 2024

Availability

Academic Institutions and Nonprofits only

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Dyn1 D406A

Plasmid

#198341

Purpose

Mammalian expression plasmid of GFP-chimera proteins.

Depositor

Justin Taraska

Article

Jimah et al Dev Cell. 2024 Apr 23:S1534-5807(24)0023

Insert

Dynamin1 (DNM1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

D406A

Promoter

CMV

Available Since

April 24, 2024

Availability

Academic Institutions and Nonprofits only

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pAS_4xKBstTyrT(CUA)_EF1_sfGFP 102TAG 150TAA

Plasmid

#174895

Purpose

amber and ochre dual suppression reporter sfGFP 102TAG 150 TAA expression, with BstTyr(CUA) amber suppressor tRNA cassette

Depositor

Simon Elsaesser

Article

Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

Insert

sfGFP

Expression

Mammalian

Mutation

102TAG 150TAA in sfGFP

Promoter

EF1

Available Since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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miR5 HsATP10B

Plasmid

#204481

Purpose

transfer plasmid for lentiviral vector production with miR for Hs ATP10B

Depositor

Veerle Baekelandt

Article

Wouters et al Biochim Biophys Acta Mol Cell Res. 2024

Insert

ATP10B (ATP10B Human)

Use

Lentiviral

Promoter

SFFV

Available Since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CAGGS-Flex/3'USS(103)-GFP

Plasmid

#197890

Purpose

Can be used to generate AAV virus that will express GFP in the presence of Cre including the shortage of a unilateral spacer sequence(USS)

Depositor

Kazuto Kobayashi

Article

Matsushita et al Cell Rep Methods. 2023 Jan 18;3(2):10039

Insert

GFP

Use

AAV

Mutation

N/A

Promoter

CAG

Available Since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CAGGS-Flex/3'USS(553)-GFP

Plasmid

#197888

Purpose

Can be used to generate AAV virus that will express GFP in the presence of Cre including the shortage of a unilateral spacer sequence(USS)

Depositor

Kazuto Kobayashi

Article

Matsushita et al Cell Rep Methods. 2023 Jan 18;3(2):10039

Insert

GFP

Use

AAV

Mutation

N/A

Promoter

CAG

Available Since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CAGGS-Flex/3'USS(285)-GFP

Plasmid

#197889

Purpose

Can be used to generate AAV virus that will express GFP in the presence of Cre including the shortage of a unilateral spacer sequence(USS)

Depositor

Kazuto Kobayashi

Article

Matsushita et al Cell Rep Methods. 2023 Jan 18;3(2):10039

Insert

GFP

Use

AAV

Mutation

N/A

Promoter

CAG

Available Since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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MEN2A-MAC

Plasmid

#139634

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Yadav et al iScience. 2020 Feb 21;23(2):100871. doi:

Insert

MEN2A (RET Human)

Tags

StrepIII-HA-BirA*

Expression

Mammalian

Mutation

C634W

Promoter

CMV

Available Since

May 13, 2020

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(Rictor-g1)-PGKpuroBFP-W

Plasmid

#105041

Purpose

Lentiviral gRNA plasmid targeting mouse Rictor , co-expression of TagBFP

Depositor

Kosuke Yusa

Article

Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

Insert

Rictor (Rictor Mouse)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(Rictor-g2)-PGKpuroBFP-W

Plasmid

#105042

Purpose

Lentiviral gRNA plasmid targeting mouse Rictor , co-expression of TagBFP

Depositor

Kosuke Yusa

Article

Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

Insert

Rictor (Rictor Mouse)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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