We narrowed to 11,283 results for: Vars;

1. pAc5.1B-EGFP-DmGW182-dsRNAres_L

   Plasmid

   #146831

   Purpose

   Insect Expression of DmGW182-dsRNAres

   Depositor

   Elisa Izaurralde

   Article

   Huntzinger et al EMBO J. 2010 Dec 15;29(24):4146-60. doi:

   Insert

   DmGW182-dsRNAres (gw Fly)
   

   Expression

   Insect

   Mutation

   Two non silent mutations V704A and N799S and one …

   Available Since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA-Prom1 Del AA 6

   Plasmid

   #184024

   Purpose

   Mammalian Flag tagged expression clone for the mouse Prom1 splicing variant SV8 (GenBank accession BC028286). Deletion of amino acids 689 to 694.

   Depositor

   Peter Stoilov

   Article

   Matalkah et al PLoS One. 2022 Oct 10;17(10):e0274958. d

   Insert

   Prom1 SV8 Del AA 6 (Prom1 Mouse)
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Deletion of amino acids 689 to 694

   Available Since

   May 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA-Prom1 D-1

   Plasmid

   #184013

   Purpose

   Mammalian Flag tagged expression clone for the mouse Prom1 splicing variant SV8 (GenBank accession BC028286). Deletion of amino acids 685-706.

   Depositor

   Peter Stoilov

   Article

   Matalkah et al PLoS One. 2022 Oct 10;17(10):e0274958. d

   Insert

   Prom1 SV8(-Ex19a) D-1 (Prom1 Mouse)
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Deletion of amino acids 685-706.

   Available Since

   May 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAc5.1B-EGFP-DmHelz-E958Q_AH

   Plasmid

   #148884

   Purpose

   Insect Expression of DmHelz-E958Q

   Depositor

   Elisa Izaurralde

   Article

   Hanet et al Life Sci Alliance. 2019 Sep 30;2(5). pii

   Insert

   DmHelz-E958Q (CG9425 Fly)
   

   Expression

   Insect

   Mutation

   two silent an two non silent G1374S, R2076G mutat…

   Available Since

   April 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAc5.1B-EGFP-DmHelz_1213-2103_AH

   Plasmid

   #148883

   Purpose

   Insect Expression of DmHelz_1213-2103

   Depositor

   Elisa Izaurralde

   Article

   Hanet et al Life Sci Alliance. 2019 Sep 30;2(5). pii

   Insert

   DmHelz_1213-2103 (CG9425 Fly)
   

   Expression

   Insect

   Mutation

   two silent an two non silent G1374S, R2076G mutat…

   Available Since

   March 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1-myc-TRBP-dsRBD2

   Plasmid

   #180602

   Purpose

   Expressing various parts of TRBP

   Depositor

   Kumiko Ui-Tei

   Article

   Takahashi et al Nucleic Acids Res. 2018 Sep 28;46(17):91

   Insert

   TRBP (TARBP2 Human)
   

   Tags

   Myc

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1-myc-TRBP-dsRBD1

   Plasmid

   #180601

   Purpose

   Expressing various parts of TRBP

   Depositor

   Kumiko Ui-Tei

   Article

   Takahashi et al Nucleic Acids Res. 2018 Sep 28;46(17):91

   Insert

   TRBP (TARBP2 Human)
   

   Tags

   Myc

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1-TRBP-L2+D2+L3-myc/His

   Plasmid

   #180600

   Purpose

   Expressing various parts of TRBP

   Depositor

   Kumiko Ui-Tei

   Article

   Takahashi et al Nucleic Acids Res. 2018 Sep 28;46(17):91

   Insert

   TRBP (TARBP2 Human)
   

   Tags

   His and Myc

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAc5.1B-lambdaN-HA-DmAGO1-F594V-siRNAres_F

   Plasmid

   #146331

   Purpose

   Insect Expression of DmAGO1-F594V

   Depositor

   Elisa Izaurralde

   Article

   Eulalio et al RNA. 2009 Jun;15(6):1067-77. doi: 10.126

   Insert

   DmAGO1-F594V (AGO1 Fly)
   

   Expression

   Insect

   Mutation

   variation with 2 non silent mutations compared to…

   Available Since

   Feb. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAc5.1B-lambdaN-HA-DmAGO1-F2V2_A

    Plasmid

    #145896

    Purpose

    Insect Expression of DmAGO1-F2V2

    Depositor

    Elisa Izaurralde

    Article

    Eulalio et al Nat Struct Mol Biol. 2008 Apr . 15(4):34

    Insert

    DmAGO1-F2V2 (AGO1 Fly)
    

    Expression

    Insect

    Mutation

    variation with 2 non silent mutations compared to…

    Available Since

    Feb. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAc5.1B-lambdaN-HA-DmAGO1-F594V_A

    Plasmid

    #145897

    Purpose

    Insect Expression of DmAGO1-F594V

    Depositor

    Elisa Izaurralde

    Article

    Eulalio et al Nat Struct Mol Biol. 2008 Apr . 15(4):34

    Insert

    DmAGO1-F594V (AGO1 Fly)
    

    Expression

    Insect

    Mutation

    variation with 2 non silent mutations compared to…

    Available Since

    Feb. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAc5.1B-lambdaN-HA-DmAGO1-siRNAres_A

    Plasmid

    #145899

    Purpose

    Insect Expression of DmAGO1-siRNAres

    Depositor

    Elisa Izaurralde

    Article

    Eulalio et al Nat Struct Mol Biol. 2008 Apr . 15(4):34

    Insert

    DmAGO1-siRNAres (AGO1 Fly)
    

    Expression

    Insect

    Mutation

    variation with 2 non silent mutations compared to…

    Available Since

    Feb. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. bdSUMO-5.3-GFP-HiBit

    Plasmid

    #171014

    Purpose

    For bacterial expression of bdSUMO tagged muGFP with 5.3 peptide and NanoLuc complementation peptide variant #86

    Depositor

    Angus Johnston

    Article

    Teo et al Nat Commun. 2021 Jun 17;12(1):3721. doi:

    Insert

    14x-His-bdSUMO-5.3-muGFP-HiBiT
    

    Use

    Luciferase

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. EGFP-acVHH (Y34W)-PB

    Plasmid

    #171072

    Purpose

    A COSMO mutant (Y34W) responds to caffeine to induce dimerization of a phospholipid-binding polybasic domain with subsequent plasma membrane translocation.

    Depositor

    Yubin Zhou

    Article

    Wang et al Adv Sci (Weinh). 2020 Dec 13;8(3):200214

    Insert

    anti-caffeine nanobody variant
    

    Tags

    modified STIM1–PB tag

    Expression

    Mammalian

    Mutation

    Y34W

    Promoter

    CMV

    Available Since

    July 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. EGFP-acVHH (Y34F)-PB

    Plasmid

    #171071

    Purpose

    A COSMO mutant (Y34F) responds to caffeine to induce dimerization of a phospholipid-binding polybasic domain with subsequent plasma membrane translocation.

    Depositor

    Yubin Zhou

    Article

    Wang et al Adv Sci (Weinh). 2020 Dec 13;8(3):200214

    Insert

    anti-caffeine nanobody variant
    

    Tags

    modified STIM1–PB tag

    Expression

    Mammalian

    Mutation

    Y34F

    Promoter

    CMV

    Available Since

    July 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. EGFP–acVHH (Y104F)–PB

    Plasmid

    #169683

    Purpose

    A COSMO mutant (Y104F) responds to caffeine to induce dimerization of a phospholipid-binding polybasic domain with subsequent plasma membrane translocation.

    Depositor

    Yubin Zhou

    Article

    Wang et al Adv Sci (Weinh). 2020 Dec 13;8(3):200214

    Insert

    anti-caffeine nanobody variant
    

    Tags

    modified STIM1–PB tag

    Expression

    Mammalian

    Mutation

    Y104F

    Promoter

    CMV

    Available Since

    July 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAc5.1B-lambdaN-HA-DmAGO1-F629V_A

    Plasmid

    #145898

    Purpose

    Insect Expression of DmAGO1-F629V

    Depositor

    Elisa Izaurralde

    Article

    Eulalio et al Nat Struct Mol Biol. 2008 Apr . 15(4):34

    Insert

    DmAGO1-F629V (AGO1 Fly)
    

    Expression

    Insect

    Mutation

    V315A, F629V, H913R - variation with 2 non silent…

    Available Since

    May 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGG422_UV5

    Plasmid

    #165607

    Purpose

    Vector for expression of the SpCas9 KG variant with sgRNA in E. coli: KG(SpCas9, D1332K/R1333G) and UV5-sgRNA (hEGFP spacer)

    Depositor

    Marcus Noyes

    Article

    Goldberg et al Nat Commun. 2021 Jan 13;12(1):349. doi:

    Insert

    lacUV5 driving Streptococcus pyogenes Cas9 KG(D1332K/R1333G) and hEGFP-sgRNA
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial

    Mutation

    D1332K and R1333G mutations in SpCas9

    Promoter

    lacUV5 driving Cas9 KG and UV5 driving sgRNA

    Available Since

    April 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET51b-His-TEV-SNAP-tag_dead_mt

    Plasmid

    #167270

    Purpose

    Overexpression of the dead variant (C145A) of SNAP-tag in E. coli

    Depositor

    Kai Johnsson

    Article

    Wilhelm et al Biochemistry. 2021 Aug 24;60(33):2560-25

    Insert

    SNAP-tag
    

    Tags

    His x10

    Expression

    Bacterial

    Mutation

    C145A

    Promoter

    T7

    Available Since

    April 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET51b-His-TEV-SNAP-tag_fast_dead_mt

    Plasmid

    #167272

    Purpose

    Overexpression of the fast (E30R) dead variant (C145A) of SNAP-tag in E. coli

    Depositor

    Kai Johnsson

    Article

    Wilhelm et al Biochemistry. 2021 Aug 24;60(33):2560-25

    Insert

    SNAPf-tag
    

    Tags

    His x10

    Expression

    Bacterial

    Mutation

    E30R-C145A

    Promoter

    T7

    Available Since

    April 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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21. pcDNA-GFP-EBA175-Flag(P1424G)

    Plasmid

    #155128

    Purpose

    Expresses N-terminally GFP-tagged EBA175 P1424G variant (residues 1284-1462) from pcDNA3.1

    Depositor

    Sinisa Urban

    Article

    Gandhi et al Cell Chem Biol. 2020 Aug 31. pii: S2451-

    Insert

    PfEBA175
    

    Tags

    Flag, GFP, and signal peptide (residues 1 to 32) …

    Expression

    Mammalian

    Mutation

    P1424G; insert codes for EBA175 residues 1284 to …

    Promoter

    CMV

    Available Since

    Nov. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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22. pcDNA-HA-PfROM4 (H578A)

    Plasmid

    #154858

    Purpose

    Expresses N-terminally HA-tagged PfROM4 variant H578A (codon optimized for mammalian expression) from pcDNA3.1

    Depositor

    Sinisa Urban

    Article

    Gandhi et al Cell Chem Biol. 2020 Aug 31. pii: S2451-

    Insert

    PfROM4 (PF3D7_0506900 Synthetic)
    

    Tags

    triple HA

    Expression

    Mammalian

    Mutation

    Histidine 578 changed to Alanine

    Promoter

    CMV

    Available Since

    Nov. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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23. pcDNA-HA-PfROM4 (G470A/G471N)

    Plasmid

    #154859

    Purpose

    Expresses N-terminally HA-tagged PfROM4 variant G470A/G471N (codon optimized for mammalian expression) from pcDNA3.1

    Depositor

    Sinisa Urban

    Article

    Gandhi et al Cell Chem Biol. 2020 Aug 31. pii: S2451-

    Insert

    PfROM4 (PF3D7_0506900 Synthetic)
    

    Tags

    triple HA

    Expression

    Mammalian

    Mutation

    Glycine 470 changed to Alanine and Glycine 471 ch…

    Promoter

    CMV

    Available Since

    Nov. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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24. pcDNA-HA-PfROM1 (A144G/N145G)

    Plasmid

    #154873

    Purpose

    Expresses N-terminally HA-tagged PfROM1 variant A144G/N145G (codon optimized for mammalian expression) from pcDNA3.1

    Depositor

    Sinisa Urban

    Article

    Gandhi et al Cell Chem Biol. 2020 Aug 31. pii: S2451-

    Insert

    PfROM1 (PF3D7_0506900 Synthetic)
    

    Tags

    triple HA

    Expression

    Mammalian

    Mutation

    Alanine 144 changed to Glycine and Asparagine 145…

    Promoter

    CMV

    Available Since

    Nov. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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25. pET15-His-∆NGlpG(C104A/M247C)

    Plasmid

    #155133

    Purpose

    Expresses N-terminally His-tagged E. coli GlpG C104A/M247C variant (residues 87-276) from pET15b

    Depositor

    Sinisa Urban

    Article

    Cho et al Nat Struct Mol Biol. 2019 Oct;26(10):910

    Insert

    GlpG (glpG Escherichia coli)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    C104A/M247C, insert codes for GlpG residues 87-276

    Promoter

    T7

    Available Since

    Sept. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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26. pET28b-FOXP2_P1

    Plasmid

    #122645

    Purpose

    Expresses truncated variant (aa271-aa714) of human FOXP2 in bacterial cells

    Depositor

    Hendrik Dietz

    Article

    Haussermann et al Angew Chem Int Ed Engl. 2019 Jun 3;58(23

    Insert

    Homo sapiens forkhead box P2 (FOXP2 Human)
    

    Tags

    His-tag and Strep-tag II

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pET28b-FOXP2_P3

    Plasmid

    #122647

    Purpose

    Expresses truncated variant (aa367-aa714) of human FOXP2 in bacterial cells

    Depositor

    Hendrik Dietz

    Article

    Haussermann et al Angew Chem Int Ed Engl. 2019 Jun 3;58(23

    Insert

    Homo sapiens forkhead box P2 (FOXP2 Human)
    

    Tags

    His-tag and Strep-tag II

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pET28b-FOXP2_P4

    Plasmid

    #122648

    Purpose

    Expresses truncated variant (aa503-aa714) of human FOXP2 in bacterial cells

    Depositor

    Hendrik Dietz

    Article

    Haussermann et al Angew Chem Int Ed Engl. 2019 Jun 3;58(23

    Insert

    Homo sapiens forkhead box P2 (FOXP2 Human)
    

    Tags

    His-tag and Strep-tag II

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pTH862-CEN-slowmaxRLuc/minCFLuc

    Plasmid

    #115371

    Purpose

    Expresses translation initiation-controlled Renilla luciferase and translation elongation-controlled firefly luciferase

    Depositor

    Tobias von der Haar

    Inserts

    Firefly Luciferase (codon minimised)
    Gcn4 5'-UTR + Renilla luciferase (codon optimised)
    

    Expression

    Yeast

    Mutation

    All codons have been changed for the most favoura…

    Promoter

    ADH1 and TDH3

    Available Since

    June 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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30. pCEP4-HA-Flag-natT1R2 d835

    Plasmid

    #113959

    Purpose

    mammalian expression plasmid for FLAG-tagged human T1R2 with signal peptide of influenza hemagglutinin; expression-enhanced variant

    Depositor

    Erik Procko

    Article

    Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061

    Insert

    T1R2 (TAS1R2 Human)
    

    Tags

    FLAG and Signal/leader sequence from influenza he…

    Expression

    Mammalian

    Mutation

    residues 22-834

    Promoter

    cmv

    Available Since

    Feb. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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