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Addgene

We narrowed to 8,430 results for: BLI

Showing: 6881 - 6900 of 8430 results
  1. pLP-hSox2-ires-gfp

    Plasmid
    #49391
    Purpose
    mammalian bicistronic expression of human Sox2 and EGFP
    Depositor
    Insert
    Sox2 (SOX2 Human)
    Use
    Cre/Lox; Acceptor vector
    Tags
    6xHN, EGFP, and IRES
    Expression
    Mammalian
    Mutation
    TGA (stop) to CCA (Pro) at nt 1027 with respect t…
    Promoter
    CMV
    Available since
    Dec. 2, 2013
    Availability
    Academic Institutions and Nonprofits only
  2. DNMT3B (3QKJ)

    Plasmid
    #32044
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Insert
    DNMT3B (DNMT3B Human)
    Use
    Tags
    His (with TEV cleavage)
    Expression
    Bacterial
    Mutation
    PWWP domain only (amino acids 293-442)
    Promoter
    Available since
    Feb. 22, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits
  3. AAV-SYN-PIK3CD

    Plasmid
    #203730
    Purpose
    AAV vector plasmid expressing human phosphoinositide 3-kinase catalytic subunit delta (PIK3CD) under the human synapsin (SYN) promoter
    Insert
    PIK3CD (PIK3CD Human)
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Human synapsin promoter
    Available since
    July 27, 2023
    Availability
    Academic Institutions and Nonprofits only
  4. Myog'-2A-mCherryNLS-PGK-Purodtk

    Plasmid
    #69547
    Purpose
    Donor vector for homologous recombination to insert a 2A-mCherry-NLS cassette in place of the stop codon of myogenin in the mouse genome.
    Depositor
    Inserts
    2A-mCherry-NLS
    Myogenin 5' homology arm (Myog Mouse)
    Myogenin 3' homology arm (Myog Mouse)
    Puro-delta-tk
    Use
    Mouse Targeting
    Tags
    2x nuclear localization signal and PVT1-2A "…
    Expression
    Mutation
    Silent V205V mutation in myogenin gene to prevent…
    Promoter
    Available since
    April 12, 2019
    Availability
    Academic Institutions and Nonprofits only
  5. pFP.R375

    Plasmid
    #138245
    Purpose
    Expresses the split mCherry reporter interrupted by the NrdA-2 intein (in cis)
    Depositor
    Inserts
    araC (araC )
    mCherryN(SNL).NrdA-2*.(CNE)mCherryC.H6
    Use
    Synthetic Biology
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Promoter
    P(araBAD) and iPC
    Available since
    March 25, 2020
    Availability
    Academic Institutions and Nonprofits only
  6. CD44I-c001

    Plasmid
    #175448
    Purpose
    Protein expression in bacterial cells (as inclusion bodies). CD44 Link module, M63-N172.
    Depositor
    Insert
    CD44 (CD44 Human)
    Use
    Tags
    His6-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
  7. pBABE.puro/TO/Flag/tGFP-HuR.wt

    Plasmid
    #110349
    Purpose
    Doxycycline inducible mammalian retroviral expression of HuR (wild type) with FLAG and turboGFP tags
    Insert
    ELAV like RNA binding protein 1 (ELAVL1 Human)
    Use
    Retroviral
    Tags
    FLAG and tGFP
    Expression
    Mammalian
    Mutation
    Promoter
    gag
    Available since
    Nov. 6, 2018
    Availability
    Academic Institutions and Nonprofits only
  8. pdCas9-sgRNA-com-BoxB-vector

    Plasmid
    #132556
    Purpose
    Expresses dCas9-DMNT3a-DMNT3l fusion protein, lambda N22-KRAB fusion protein, and sgRNA scaffold with com and BoxB replacing the Tetraloop and the loop 2 respectively.
    Depositor
    Inserts
    dCas9-DMNT3a-DMNT3l (DNMT3A Synthetic)
    Lambda N22-KRAB
    sgRNA acaffold with com and BoxB replacing the Tetraloop and the loop 2.
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 23, 2022
    Availability
    Academic Institutions and Nonprofits only
  9. HDGF2

    Plasmid
    #32021
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Insert
    HDGF2 (HDGFL2 Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    PWWP domain only (amino acid residues 1-93)
    Promoter
    T7
    Available since
    Jan. 3, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits
  10. pFP.R271

    Plasmid
    #138220
    Purpose
    Expresses the split mCherry reporter interrupted by the SspDnaX artificially mini-intein (in cis)
    Depositor
    Inserts
    araC (araC )
    mCherryN(IDE).SspDnaX.(CHT)mCherryC.H6
    Use
    Synthetic Biology
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Promoter
    P(araBAD) and iPC
    Available since
    May 7, 2020
    Availability
    Academic Institutions and Nonprofits only
  11. pSIREN-RetroQ-BPTF-sh28

    Plasmid
    #73669
    Purpose
    pSIREN-RetroQ vector containing shRNA sequence to BPTF
    Depositor
    Insert
    shRNA 28 to BPTF (BPTF, Bptf Human, Mouse)
    Use
    Retroviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 22, 2016
    Availability
    Industry, Academic Institutions, and Nonprofits
  12. pBABE.puro/TO/Flag/tGFP.HuR.S221D

    Plasmid
    #110351
    Purpose
    Doxycycline inducible mammalian retroviral expression of HuR (S221D) with FLAG and turboGFP tags
    Insert
    ELAV like RNA binding protein 1 (ELAVL1 Human)
    Use
    Retroviral
    Tags
    FLAG and tGFP
    Expression
    Mammalian
    Mutation
    S221D, P237S
    Promoter
    gag
    Available since
    Nov. 6, 2018
    Availability
    Academic Institutions and Nonprofits only
Showing: 6881 - 6900 of 8430 results