We narrowed to 9,391 results for: BLI

1. 6xO/S luc

   Plasmid

   #69445

   Purpose

   detection/assessment of OCT4 and SOX2 transcription factor activity

   Depositor

   Lisa Dailey

   Insert

   minimal fgf4 promoter and 6XOS tandem repeat of composite OCT4/Sox2 binding site from FGF4 enhancer
   

   Use

   Luciferase

   Expression

   Mammalian

   Available Since

   May 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. Hs.KRAS4b G13D

   Plasmid

   #83133

   Purpose

   Gateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), G13D mutation

   Depositor

   Dominic Esposito

   Insert

   KRAS (KRAS Human)
   

   Use

   Gateway entry clone

   Mutation

   G13D

   Available Since

   Sept. 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. ACACA

   Plasmid

   #39083

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   ACACA (ACACA Human)
   

   Tags

   His Tev

   Expression

   Bacterial

   Mutation

   codon-optimized

   Available Since

   March 18, 2013

   Availability

   Academic Institutions and Nonprofits only

4. SARS-CoV-2 Spike-d18

   Plasmid

   #164583

   Purpose

   A mammalian expression vector to express codon-optimized SARS-CoV-2 Spike protein, with a C terminal truncation of 18 amino acids to improve pseudoparticle generation.

   Depositor

   Jason Sheltzer

   Insert

   SARS-CoV-2 Spike (S SARS-CoV-2)
   

   Expression

   Mammalian

   Mutation

   Deleted amino acids 1256-1273

   Promoter

   EF1a

   Available Since

   Jan. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. Lamin A (G465D)-mRFP

   Plasmid

   #124271

   Purpose

   Lamin A mutant (G465D), C-terminally tagged with mRFP. Mutation associated with FPLD lipodystrophy.

   Depositor

   Eric Schirmer

   Insert

   LMNA (G465D) (LMNA Human)
   

   Tags

   mRFP

   Expression

   Mammalian

   Mutation

   LMNA (G465D)

   Available Since

   May 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. EEF1A2 in pENTR

   Plasmid

   #216585

   Purpose

   gateway cloning donor vector containing EEF1A2

   Depositor

   Douglas Allan, Christopher Loewen

   Insert

   EEF1A2 (EEF1A2 Human)
   

   Use

   Gateway entry plasmid

   Available Since

   March 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pOET5 CVB3-VLP

   Plasmid

   #218146

   Purpose

   Forms coxsackievirus 3-like particle in insect cells by expression of VP1–VP4 polyprotein (GeneID M33854.1) and 3CD protease (GeneID M33854.1).

   Depositor

   Minna Hankaniemi

   Inserts

   VP1–VP4
   3CD
   

   Expression

   Insect

   Promoter

   CMV and Polyhedrin

   Available Since

   May 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGWB2-CYCD3;1

   Plasmid

   #140750

   Purpose

   Inducing plant cells to enter mitosis

   Depositor

   Bo Liu

   Article

   Xu et al New Phytol. 2019 Nov 2. doi: 10.1111/nph

   Insert

   Cyclin D3;1 (CYCD3;1 Mustard Weed)
   

   Expression

   Plant

   Promoter

   35S

   Available Since

   May 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLPS-hSox2-gfp

   Plasmid

   #49390

   Purpose

   mammalian expression of human Sox2 fused to EGFP

   Depositor

   Randall McKinnon

   Insert

   Sox2 (SOX2 Human)
   

   Use

   Cre/Lox; Acceptor vector

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   TGA (stop) to CCA (Pro) at nt 1027 with respect t…

   Promoter

   CMV

   Available Since

   Nov. 21, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. Hs.KRAS4b G12C

    Plasmid

    #83130

    Purpose

    Gateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), G12C mutation

    Depositor

    Dominic Esposito

    Insert

    KRAS (KRAS Human)
    

    Use

    Gateway entry clone

    Mutation

    G12C

    Available Since

    Sept. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. AICSDP-69: NPM1-mTagRFP-T

    Plasmid

    #124608

    Purpose

    Homology arms and linker-mTagRFP-T sequence for C-terminus tagging of human NPM1

    Depositor

    Allen Institute for Cell Science

    Insert

    NPM1 Homology Arms with linker-mTagRFP-T (NPM1 Human)
    

    Use

    CRISPR; Donor template

    Expression

    Mammalian

    Available Since

    May 13, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. Hs.KRAS4a G12D

    Plasmid

    #83168

    Purpose

    Gateway ORF Entry clone of human KRAS4A [NM_033360.3] with stop codon (for native or N-terminal fusions), G12D mutation

    Depositor

    Dominic Esposito

    Insert

    KRAS (KRAS Human)
    

    Use

    Gateway entry clone

    Mutation

    G12D

    Available Since

    Sept. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. 4IKP

    Plasmid

    #60080

    Purpose

    Baculovirus expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    CARM1:JMC049-A06:C226836 (CARM1 Human)
    

    Use

    Baculovirus expression

    Tags

    6xHis

    Mutation

    See comments

    Promoter

    polyhedrin

    Available Since

    Oct. 22, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

14. pCAGGS-N2c(G)

    Plasmid

    #73481

    Purpose

    N2c Glycoprotein expression vector

    Depositor

    Thomas Jessell

    Article

    Reardon et al Neuron. 2016 Jan 19. pii: S0896-6273(16)

    Insert

    N2c(G)
    

    Expression

    Mammalian

    Available Since

    March 21, 2016

    Availability

    Academic Institutions and Nonprofits only

15. pET28a-UBC9

    Plasmid

    #228949

    Purpose

    express a fragment of the wild-type human UBC9 (residues 2-158 of UniProt P63279 supplemented with the N-terminal thrombin-cleavable MGHHHHHHSSGLVPR|G- tag)

    Depositor

    Marcin Suskiewicz

    Insert

    UBC9 (UBE2I Human)
    

    Tags

    His tag and thrombine cleavage site : MGHHHHHHSSG…

    Expression

    Bacterial

    Promoter

    T7-lac

    Available Since

    May 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-hSyn-GluA1-scFv-mNeon-KDEL

    Plasmid

    #230056

    Purpose

    AAV plasmid that expresses a single chain variable fragment (scFv) for anti-GluA1 tagged with mNeon. Encodes an ER retrieval motif (KDEL) at C-terminus of mNeon.

    Depositor

    Matthew Kennedy

    Insert

    Anti-GluA1 single chain variable fragment (scFv)
    

    Use

    AAV

    Tags

    ER retrieval motif (KDEL) and mNeon

    Expression

    Mammalian

    Promoter

    human synapsin

    Available Since

    Jan. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. DDX3X in pENTR

    Plasmid

    #216572

    Purpose

    gateway cloning donor vector containing DDX3X

    Depositor

    Douglas Allan, Christopher Loewen

    Insert

    DDX3X (DDX3X Human)
    

    Use

    Gateway entry plasmid

    Available Since

    March 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-hSyn-DIO-HA-rM3D(Gs)-IRES-mCitrine

    Plasmid

    #50456

    Purpose

    Double floxed Gs-coupled rM3D-IRES-mCitrine under the control of human synapsin promoter

    Depositor

    Bryan Roth

    Insert

    rM3D-IRES-mCitrine (CHRM3 Human)
    

    Use

    AAV

    Tags

    HA

    Mutation

    See supplemental documents for DREADD mutations

    Promoter

    human Synapsin 1

    Available Since

    April 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLV hUbC- VP64-dCas9-VP64-T2A- GFP(No LoxP sites)

    Plasmid

    #66708

    Purpose

    expresses VP64-dCas9-VP64, same plasmid as Addgene 59791 but with LoxP sites removed

    Depositor

    Charles Gersbach

    Insert

    S.Pyogenes VP64-dCas9-VP64
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    VP64

    Mutation

    D10A and H840A

    Promoter

    hUbC

    Available Since

    April 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGWB660-CYCD3;1

    Plasmid

    #140751

    Purpose

    Inducing plant cells to enter mitosis

    Depositor

    Bo Liu

    Article

    Xu et al New Phytol. 2019 Nov 2. doi: 10.1111/nph

    Insert

    Cyclin D3;1 (CYCD3;1 Mustard Weed)
    

    Tags

    TagRFP

    Expression

    Plant

    Promoter

    CaMV 35 promoter

    Available Since

    May 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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