We narrowed to 7,572 results for: Lif

1. pWPT-/CGA-mEGFP-IRES-mCherry

   Plasmid

   #49233

   Purpose

   Lentiviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.

   Depositor

   Clifford Wang

   Article

   Ferreira et al Proc Natl Acad Sci U S A. 2013 Jul 9;110

   Inserts

   mEGFP
   mCherry
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   SfuI site to create C-terminal fusions

   Expression

   Mammalian

   Mutation

   Promoter

   EF1-short

   Available since

   Dec. 5, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. Sumo3 PolQM1 deltains2

   Plasmid

   #78460

   Purpose

   Inducible expression in E. coli

   Depositor

   Sylvie Doublie, Susan Wallace

   Article

   Hogg et al J Mol Biol. 2011 Jan 21;405(3):642-52. d

   Insert

   Polymerase theta (POLQ Human)
   

   Use

   Tags

   His and SUMO3

   Expression

   Bacterial

   Mutation

   delta 1-1791; delta 2264-2315; Q2513R (see deposi…

   Promoter

   Available since

   July 28, 2016

   Availability

   Academic Institutions and Nonprofits only

3. pMpGWB119

   Plasmid

   #68573

   Purpose

   Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

   Depositor

   Takayuki Kohchi

   Article

   Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

   Type

   Empty backbone

   Use

   Tags

   Modified EAR motif plant-specific repression doma…

   Expression

   Plant

   Mutation

   Promoter

   Cauliflower mosaic virus 35S promoter

   Available since

   June 24, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCru5-/CGA-mEGFP-IRES-mCherry

   Plasmid

   #49224

   Purpose

   Retroviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.

   Depositor

   Clifford Wang

   Article

   Ferreira et al Proc Natl Acad Sci U S A. 2013 Jul 9;110

   Inserts

   mEGFP
   mCherry
   

   Use

   Retroviral and Synthetic Biology

   Tags

   SfuI site to create C-terminal fusions

   Expression

   Mammalian

   Mutation

   Promoter

   Viral LTR (or CMV)

   Available since

   Dec. 5, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDONR221-ARF6

   Plasmid

   #67314

   Purpose

   hArf6 with stop codon in Gateway Entry vector

   Depositor

   Richard Kahn

   Article

   Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

   Insert

   ARF6 (ARF6 Human)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   None

   Available since

   Oct. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

6. RCAS(A) dRhoA N19 (CT#847)

   Plasmid

   #14002

   Depositor

   Cliff Tabin

   Article

   Hartmann et al Cell. 2001 Feb 9. 104(3):341-51.

   Insert

   RhoA (Rho1 Fly)
   

   Use

   Retroviral; Avian expression

   Tags

   Expression

   Mutation

   T19N

   Promoter

   Available since

   Feb. 16, 2007

   Availability

   Academic Institutions and Nonprofits only

7. L-NARS-2

   Plasmid

   #166532

   Purpose

   scFv of a human scaffold targeting Asparaginyl-tRNA synthetase. Antigen coverage aa 1-458 of 458, full-length

   Depositor

   Susanne Gräslund

   Article

   Preger et al J Biol Chem. 2020 Oct 9;295(41):13981-13

   Insert

   single chain-Fv, scFv
   

   Use

   Recombinant antibody expression

   Tags

   3xFLAG, His6 and OmpA leader sequence

   Expression

   Bacterial

   Mutation

   Promoter

   LacZ

   Available since

   April 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. O-AARS-51

   Plasmid

   #166539

   Purpose

   scFv of a human scaffold targeting Alanyl-tRNA synthetase. Antigen coverage aa 1-455 of 968

   Depositor

   Susanne Gräslund

   Article

   Preger et al J Biol Chem. 2020 Oct 9;295(41):13981-13

   Insert

   single chain-Fv, scFv
   

   Use

   Recombinant antibody expression

   Tags

   3xFLAG, His6 and OmpA leader sequence

   Expression

   Bacterial

   Mutation

   Promoter

   LacZ

   Available since

   April 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. L-TARS-11

   Plasmid

   #166536

   Purpose

   scFv of a human scaffold targeting Threonyl-tRNA synthetase. Antigen coverage aa 320-723 of 723

   Depositor

   Susanne Gräslund

   Article

   Preger et al J Biol Chem. 2020 Oct 9;295(41):13981-13

   Insert

   single chain-Fv, scFv
   

   Use

   Recombinant antibody expression

   Tags

   3xFLAG, His6 and OmpA leader sequence

   Expression

   Bacterial

   Mutation

   Promoter

   LacZ

   Available since

   April 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. L-WARS-26

    Plasmid

    #166528

    Purpose

    scFv of a human scaffold targeting Tryptophanyl-tRNA synthetase. Antigen coverage aa 1-1471 of 471, full-length

    Depositor

    Susanne Gräslund

    Article

    Preger et al J Biol Chem. 2020 Oct 9;295(41):13981-13

    Insert

    single chain-Fv, scFv
    

    Use

    Recombinant antibody expression

    Tags

    3xFLAG, His6 and OmpA leader sequence

    Expression

    Bacterial

    Mutation

    Promoter

    LacZ

    Available since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCru5-/UAA-mEGFP-IRES-mCherry

    Plasmid

    #49225

    Purpose

    Retroviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.

    Depositor

    Clifford Wang

    Article

    Ferreira et al Proc Natl Acad Sci U S A. 2013 Jul 9;110

    Inserts

    mEGFP
    mCherry
    

    Use

    Retroviral and Synthetic Biology

    Tags

    SfuI site to create C-terminal fusions

    Expression

    Mammalian

    Mutation

    Promoter

    Viral LTR (or CMV)

    Available since

    Dec. 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pLenti6.3/V5-DEST-PKCi-A120E-ΔPB1

    Plasmid

    #56658

    Purpose

    expresses constitutively active human PKCi with a deletion on the PB1 domain

    Depositor

    Pedro Salas

    Article

    Forteza et al Biol Open. 2013 Oct 8;2(11):1264-9. doi:

    Insert

    Human PKCi (PRKCI Human)
    

    Use

    Lentiviral

    Tags

    V5/His

    Expression

    Mutation

    A120E and deletion of PB1 domain (aa 24–98)

    Promoter

    CMV

    Available since

    Sept. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

13. pFlg167

    Plasmid

    #101019

    Purpose

    fluorescent, functional, glutamate receptor subunit

    Depositor

    Thomas Hughes

    Article

    Sheridan et al Neuroscience. 2006 Aug 25;141(2):837-49.

    Insert

    GluR 1 (Gria1 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    fluorescent protein inserted into subunit

    Promoter

    cmv

    Available since

    Sept. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pp32ΔCT

    Plasmid

    #67241

    Purpose

    pp32(1-149) cloned into pProEX HTb

    Depositor

    Cynthia Wolberger

    Article

    Huyton et al Protein Sci. 2007 Jul;16(7):1308-15. Epu

    Insert

    pp32 (ANP32A Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    contains amino acid residues 1-149

    Promoter

    Available since

    May 16, 2016

    Availability

    Academic Institutions and Nonprofits only

15. pWPT-ACC/ACC-mEGFP-IRES-mCherry

    Plasmid

    #49229

    Purpose

    Lentiviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.

    Depositor

    Clifford Wang

    Article

    Ferreira et al Proc Natl Acad Sci U S A. 2013 Jul 9;110

    Inserts

    mEGFP
    mCherry
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    SfuI site to create C-terminal fusions

    Expression

    Mammalian

    Mutation

    Promoter

    EF1-short

    Available since

    Dec. 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pT7-agrBD-IV

    Plasmid

    #53440

    Purpose

    Derivative of pT7-agrBD-I with a point mutation in the agrD gene to change AIP coding region from type-I to type-IV

    Depositor

    Cynthia Collins

    Article

    Marchand et al Biotechnol Bioeng. 2013 Nov;110(11):3003

    Insert

    agrBD locus (with D29Y mutation in agrD)
    

    Use

    Synthetic Biology

    Tags

    V5 epitope, 6x HIS (not expressed on agrB or agrD…

    Expression

    Bacterial

    Mutation

    D to Y mutation of residue 29 of the agrD gene (c…

    Promoter

    T7-lac

    Available since

    June 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pDEST47-ARF4

    Plasmid

    #67455

    Purpose

    Mammalian expression of hArf4 with stop codon (native expression)

    Depositor

    Richard Kahn

    Article

    Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

    Insert

    ARF4 (ARF4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

18. pMpGWB222

    Plasmid

    #68613

    Purpose

    Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

    Depositor

    Takayuki Kohchi

    Article

    Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

    Type

    Empty backbone

    Use

    Tags

    Modified EAR motif plant-specific repression doma…

    Expression

    Plant

    Mutation

    Promoter

    Cauliflower mosaic virus 35S promoter

    Available since

    July 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMpGWB219

    Plasmid

    #68610

    Purpose

    Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

    Depositor

    Takayuki Kohchi

    Article

    Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

    Type

    Empty backbone

    Use

    Tags

    Modified EAR motif plant-specific repression doma…

    Expression

    Plant

    Mutation

    Promoter

    Cauliflower mosaic virus 35S promoter

    Available since

    June 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pFRT-TODestFLAGHA_RBPMS_KDR_HindIII

    Plasmid

    #65662

    Purpose

    FLAG-HA expression vector with RBPMS carrying mutation to introduce HindIII site and encoding silent mutation to render resistant to siRNA R2 (Farazi et al. 2014)

    Depositor

    Thomas Tuschl

    Article

    Teplova et al Q Rev Biophys. 2015 Sep 8:1-11.

    Insert

    RBPMS (RBPMS Human)
    

    Use

    Tags

    FLAG HA

    Expression

    Mammalian

    Mutation

    introduced HindIII site (final goal to exchange F…

    Promoter

    CMV, 2x Tet

    Available since

    June 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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