Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 11,283 results for: Vars;

Showing: 10381 - 10410 of 11283 results

  4. MSP2135

    Plasmid
    #72248
    Purpose
    Human expression plasmid for SpCas9-HF2 variant: CMV-T7-humanSpCas9-HF2(N497A, R661A, Q695A, Q926A, D1135E)-NLS-3xFLAG
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1
    Insert
    mammalian codon-optimized Streptococcus pyogenes Cas9 HF2(N497A/R661A/Q695A/Q926A/D1135E)-NLS-3xFlag
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Mammalian
    Mutation
    N497A, R661A, Q695A, Q926A, and D1135E
    Promoter
    CMV
    Available Since
    Jan. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    72248-plasmid-map-sequence-id-131868

    Enlarge

  9. p-spTorA-GFP-H6C

    Plasmid
    #168517
    Purpose
    GFP with the signal peptide of TorA (spTorA) in pBAD24. Includes a C-terminal HHHHHHC (6xHisC) extension.
    Depositor
    Siegfried Musser
    Article
    Bageshwar et al PLoS One. 2021 Sep 9;16(9):e0256715. doi
    Insert
    spTorA-GFP-H6C
    Tags
    6xHisC tag and spTorA (signal peptide of E. coli …
    Expression
    Bacterial
    Mutation
    The mut3 GFP variant with 5 additional mutations …
    Promoter
    araBAD
    Available Since
    Oct. 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    168517-plasmid-map-sequence-id-346120

    Enlarge

  18. pHS1950

    Plasmid
    #205983
    Purpose
    Ga0116245_10002768 T7/LacO-Cas6 + TwinStrep-Cas14 + Cas7 + His14-Cas5::pJ23119-DR-Fn spacer-DR
    Depositor
    Feng Zhang
    Article
    Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.
    Inserts
    Ga0116245_10002768 candidate type VII proteins
    Cas14
    Cas5
    CRISPR array
    Tags
    14xHis and TwinStrep-tag
    Expression
    Bacterial
    Promoter
    T7 (proteins), pJ23119 (crRNA)
    Available Since
    Nov. 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    205983-plasmid-map-sequence-id-415374

    Enlarge

  20. p2R3a-3AT

    Plasmid
    #71273
    Purpose
    Entry clone containing 3AT. Necessary to complete the transcriptional reporters by providing the poly-A tail. For use in plants and compatible with the MultiSite Gateway system
    Depositor
    Ari Pekka Mähönen
    Article
    Siligato et al Plant Physiol. 2015 Dec 7. pii: pp.01246
    Insert
    T3A ribulose-1,5-bisphosphate carboxylase 3A subunit terminator
    Use
    Gateway
    Available Since
    Dec. 7, 2015
    Availability
    Academic Institutions and Nonprofits only
    71273-plasmid-map-sequence-id-129830

    Enlarge

  23. BPK2797

    Plasmid
    #72251
    Purpose
    Human expression plasmid for SpCas9-VRQR variant: CMV-T7-humanSpCas9-VRQR(D1135V, G1218R, R1335Q, T1337R)-NLS-3xFLAG
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1
    Insert
    mammalian codon-optimized Streptococcus pyogenes Cas9 VRQR(D1135V/G1218R/R1335Q/T1337R)-NLS-3xFlag
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Mammalian
    Mutation
    D1135V, G1218R, R1335Q, and T1337R
    Promoter
    CMV
    Available Since
    Jan. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    72251-plasmid-map-sequence-id-131872

    Enlarge

  29. pVE2_11_p40S_QfDBD_VP16AD_cp_phl_DsRed_0xQUAS

    Plasmid
    #154229
    Purpose
    Encodes p40S:QfDBD-VP16AD-GFP-NLS synthetic transcription factor, activating DsRed-SKL with promoter CP_phl (no QUAS control)
    Depositor
    Arnold Driessen
    Article
    Mozsik et al Microb Cell Fact. 2019 Nov 18;18(1):203.
    Insert
    p40S:QfDBD-VP16AD-GFP-NLS
    Use
    Synthetic Biology; Fungal expression
    Tags
    GFP-NLS; DsRed-SKL
    Expression
    Bacterial
    Promoter
    p40S (AN0465 A. nidulans)
    Available Since
    July 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    154229-plasmid-map-sequence-id-306038

    Enlarge

  30. pAAV-Ef1a-FAS-NES-jRGECO1a-WPRE

    Plasmid
    #141236
    Purpose
    AAV vector with Ef1a promoter and LoxFAS sites for Cre-Off expression of jRGECO1a (jRGECO1a will not be expressed in mammalian cells that express Cre recombinase)
    Depositor
    Guohong Cui
    Article
    Meng et al Neuron. 2018 May 16;98(4):707-717.e4. do
    Insert
    NES-jRGECO1a
    Use
    AAV and Cre/Lox; Cre-off
    Tags
    6xHIS tag and nuclear export signal
    Promoter
    human elongation factor-1 alpha (EF-1 alpha)
    Available Since
    May 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    141236-plasmid-map-sequence-id-432423

    Enlarge

Showing: 10381 - 10410 of 11283 results