159,461 results

1. lentiCRISPRv2FE-ABE8e-SpRY

   Plasmid

   #213008

   Purpose

   A lentiviral vector expressing the ABE8e-SpRY base editor and an sgRNA cloning site

   Depositor

   Richard Sherwood

   Article

   Ryu et al Nat Genet. 2024 Apr 24. doi: 10.1038/s41

   Insert

   ABE8e-SpRY-D10A
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   D10A nickase variant of SpRY

   Promoter

   Ef-1a

   Available since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pBSTNAV

   Plasmid

   #45801

   Depositor

   Luc Ponchon

   Article

   Ponchon et al Nucleic Acids Res. 2013 Aug;41(15):e150.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 5, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAAV-EFS-SpCas9

   Plasmid

   #104588

   Purpose

   AAV vector expressing Myc-tagged SpCas9 under the EFS promoter

   Depositor

   Ryohei Yasuda

   Article

   Nishiyama et al Neuron. 2017 Oct 18. pii: S0896-6273(17)

   Insert

   Streptococcus pyogenes Cas9
   

   Use

   AAV and CRISPR

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   EFS

   Available since

   Dec. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pAAV_BiVIPe4_GCaMP6f

   Plasmid

   #213859

   Purpose

   AAV construct expressing GCaMP6f driven by VIP interneuron-targeting enhancer. Alias: pAAV_YWE4.2_GCaMP6f

   Depositor

   Gordon Fishell

   Article

   Furlanis et al Neuron. 2025 May 21;113(10):1525-1547.e1

   Insert

   GCaMP6f
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pBT374

   Plasmid

   #125615

   Purpose

   Expresses evoFERNY-BE4max-NG in mammalian cells

   Depositor

   David Liu

   Article

   Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

   Insert

   evoFERNY-BE4max-NG
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   H102P D104N

   Promoter

   Available since

   July 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. mRNA-CleanCap entry vector

   Plasmid

   #188249

   Purpose

   Cloning vector for generating template plasmids to transcribe mRNA, with mNeonGreen dropout following BsaI digestion

   Depositor

   Howard Chang

   Article

   Chen et al Nat Biotechnol. 2022 Jul 18. pii: 10.103

   Insert

   mNeonGreen
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   T7

   Available since

   Sept. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pT077

   Plasmid

   #137879

   Purpose

   Donor vector for integration into the human AAVS1 safe harbor locus of Doxicycline-inducible KRAB-dCas9-IRES-EGFP

   Depositor

   Lindy Barrett

   Article

   Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

   Insert

   TRE3G-KRAB-dCas9-IRES-EGFP-CAG-TetON-NeoR-SA
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAAV-CAG-iGluSnFR4s-NGR-WPRE

   Plasmid

   #234451

   Purpose

   AAV-mediated expression of glutamate sensor with improved sensitivity and slow deactivation kinetics (4s = slow); NGR vector matches or outperforms PDGFR in all conditions tested.

   Depositor

   GENIE Project

   Article

   Aggarwal et al bioRxiv 2025.03.20.643984

   Insert

   iGluSnFR4s-NGR
   

   Use

   AAV

   Tags

   IgK chain and Myc epi tag-NGR GPI anchor

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pLNCX2 ER:ras

   Plasmid

   #67844

   Purpose

   4-hydroxytamoxifen inducible ER:ras fusion protein

   Depositor

   Masashi Narita

   Article

   Young et al Genes Dev. 2009 Apr 1;23(7):798-803. doi

   Insert

   ER1a H-RasG12V fusion
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   G525R renders the HBD largely insensitive to 17ß‐…

   Promoter

   CMV

   Available since

   Aug. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. EGFP-Tau(T217E)

    Plasmid

    #187024

    Purpose

    Expresses EGFP tagged human 2N4R tau (with T217E mutation) in mammalian cells with CMV promoter

    Depositor

    George Bloom

    Article

    Rajbanshi et al Alzheimers Dement. 2023 Jan 12. doi: 10.

    Insert

    MAPT (MAPT Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Changed Threonine 217 to Glutamic acid

    Promoter

    CMV

    Available since

    Jan. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge