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Plasmid#212801PurposeConstitutive expression of 3xFLAG-tagged budding yeast Ubc7 in mammalian cellsDepositorInsertUbc7
UseTags3xFLAGExpressionMammalianMutationPromoterAvailable sinceJan. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TO-Ubc13
Plasmid#212806PurposeConstitutive or doxycycline-inducible expression of budding yeast Ubc13 in mammalian cellsDepositorInsertUbc13
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TO-Mms2
Plasmid#212807PurposeConstitutive or doxycycline-inducible expression of budding yeast Mms2 in mammalian cellsDepositorInsertMms2
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET24a-His7-NUbo-GFP
Plasmid#212719PurposeBacterial expression of His7-NUbo-GFPDepositorInsertGFP
UseTags7xHis-NUboExpressionBacterialMutationPromoterAvailable sinceJan. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET24a-NUbo-GFP-His6
Plasmid#212718PurposeBacterial expression of NUbo-GFP-His6DepositorInsertGFP
UseTags6xHis and NUboExpressionBacterialMutationPromoterAvailable sinceJan. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET30-His6-TEV-Mms2
Plasmid#212716PurposeBacterial expression of His6-Mms2, His6-tag cleavable with TEV-proteaseDepositorInsertMms2
UseTags6xHis-TEVExpressionBacterialMutationPromoterAvailable sinceJan. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYea5.1_Notch1-L24_sfGFP
Plasmid#207865PurposeYeast 2µ vector for expression of Suc2SP-Notch1-L24_100-sfGFP-GAL4DepositorInsertSuc2SP-Notch1-L24_100-sfGFP-GAL4 fusion protein
UseTagsStrep-TagIIExpressionBacterial and YeastMutationPromoterAvailable sinceDec. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pWR54
Plasmid#203330Purposeepisomal bioluminescent reporter plasmid for S. stipitisDepositorInsertsTEF1 promoter (S. stipitis) (TEF1 S. stipitis (yeast))
coHPH
ACT1 terminator (S. stipitis)
CCW12 promoter (S. stipitis)
coCBG
ADH2 terminator (S. stipitis)
ARS from S. stipitis chromosome 1
UseTagsExpressionYeastMutationPromoterAvailable sinceDec. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pYea5.1_Notch1_sfGFP
Plasmid#207801PurposeYeast 2µ vector for expression of Suc2SP-Notch1_100-sfGFP-GAL4DepositorInsertSuc2SP-Notch1_100-sfGFP-GAL4 fusion protein
UseTagsStrep-TagIIExpressionBacterial and YeastMutationPromoterTEFAvailable sinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pWR16
Plasmid#202711Purposeintegrating bioluminescent reporter plasmid for S. stipitisDepositorInsertsTEF1 promoter (S. stipitis) (TEF1 S. stipitis (yeast))
coHPH
ACT1 terminator (S. stipitis)
TDH3 promoter (S. stipitis)
coCBG
ADH2 terminator (S. stipitis)
URA3 and flanking sequence
UseTagsExpressionYeastMutationPromoterAvailable sinceOct. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJS506
Plasmid#200712PurposePromoter region from ATPase PMA1 as Pro5U promoter module for heterologous expression of plasma membrane localized transporters in yeastDepositorInsertPMA1 promoter (PMA1 Budding Yeast)
UseSynthetic Biology; Modular cloningTagsExpressionMutationdomesticated for MoClo cloning (removal of intern…PromoterAvailable sinceAug. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLV-REPORT(PGK/CMV)-GRE
Plasmid#172336PurposeGal4 Response elementDepositorInsertsmonomeric nuclear Tomato
d2eGFP
HygR
UseLentiviral and Synthetic BiologyTags3x SV40 NLSExpressionMammalianMutationPromoterPGK/CMVAvailable sinceJune 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
KAN(G418)-TET-OFF – ubiquitin – LEU – 4FLAG
Plasmid#193317PurposeDoxycycline-induced transcriptional regulation and Ubiquitin - LEU - 4xFLAG N-terminal tagging. Ubiquitin is cleaved off, resulting in an unstable protein with an N-terminal leucine.DepositorInsertUbiquitin - Leucine - 4FLAG N-terminal tagging
UseTags4FLAGExpressionBacterialMutationPromoterAvailable sinceFeb. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMM4_14
Plasmid#183062PurposePlasmid expressing a biosensor for acetic acid: BM3R1-Haa1-mTurquoise2 under RET2p and mCherry under HREsYGP1p with flanks for genome integration into HO locusDepositorInsertsmCherry
BM3R1-HAA1-mTurquoise2
UseSynthetic BiologyTagsmCherry and mTurquoise2ExpressionYeastMutationPromoterHREsYGP1p and RET2Available sinceSept. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJCble
Plasmid#185861PurposeThe CRISPR/Cas9-and-gRNA-expressing plasmid with a phleomycin-resistent selectable markerDepositorInsertPTDH3-Cas9(N); PSNR52-srRNA-TSUP4; ble
UseTagsExpressionYeastMutationNonePromoterAvailable sinceSept. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pIALD2S
Plasmid#185865PurposeKnocking out ALD6 and expressing anacetylating aldehyde dehydrogenase (Lactobacillus reuteri pduP ) in Saccharomyces cerevisiaeDepositorInsertALD6 (-125, 40)> PADH1> Lr.pduP> TPDC1-ALD6 (1054, 1749)
UseTagsExpressionYeastMutationNonePromoterAvailable sinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pfa6a mScarlet-TsCC(B)::KANMX
Plasmid#176397PurposePlasmid for cloning mScarlet fused to TsCC(B) coiled coil into yeast.DepositorInsertmScarlet-TsCC(B)
UseTagsmScarlet fused to TsCC(B)ExpressionBacterial and YeastMutationPromoternoneAvailable sinceJuly 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDIV492
Plasmid#177700PurposePlasmid expressing optimized Cas9 and NAT marker. Compatible with CTG-clade yeast species.DepositorInsertsCas9
Nat
UseCRISPRTagsSV40ExpressionBacterial and YeastMutationPromoterRHR2p (Debaryomyces hansenii ) and TEF1p (Ashbya …Available sinceJuly 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pYL237
Plasmid#173194PurposeCRISPR donor plasmid for making the RNA-binding mutant EZH2 control cell line. It contains a puromycin resistance gene and an mCherry geneDepositorInsertEZH2 RNA-binding mutant
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceApril 28, 2022AvailabilityAcademic Institutions and Nonprofits only