We narrowed to 8,430 results for: BLI
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Plasmid#222016PurposeExpress human PHOSPHO2 protein (C-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-fusion protein (ENLYFQGS-WSHPQFEK-(GGGS)2-GGSA-WSHPQFEK)) in mammalian cellDepositorInsertPHOSPHO2 (PHOSPHO2 Human)
UseTagsTEV cleavable site and Twin-Strep-tagExpressionMammalianMutationdeleted stop codon (TAA)Promoterchicken β-actin promoterAvailable sinceJan. 8, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGEX-PHOSPHO2-TEV-Twin-Strep
Plasmid#222017PurposeExpress human PHOSPHO2 protein (C-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-fusion protein (ENLYFQGS-WSHPQFEK-(GGGS)2-GGSA-WSHPQFEK)) in E. coliDepositorInsertPHOSPHO2 (PHOSPHO2 Human)
UseTagsTEV cleavable site and Twin-Strep-tagExpressionBacterialMutationdeleted stop codon (TAA)Promoterlac promoterAvailable sinceJan. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
AICSDP-167:CDH5-mEGFP
Plasmid#217425PurposeHomology arms and mEGFP sequence for C-terminus tagging of human cadherin 5DepositorInsertCDH5 Homology Arms with mEGFP (CDH5 Human)
UseCRISPR; Donor templateTagsmEGFPExpressionMutationhomology arms contain point mutations to disrupt …PromoterAvailable sinceNov. 25, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCDB179-Cut190*SS
Plasmid#218706PurposeEncodes codon optimized Cut190*SSenzyme for bacterial expressionDepositorInsertCut190*SS
UseTags10xHisExpressionBacterialMutationPromoterAvailable sinceNov. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDB179-LCC-ICCG
Plasmid#218687PurposeEncodes codon optimized LCC-ICCG enzyme for bacterial expressionDepositorInsertLCC-ICCG
UseTags10xHisExpressionBacterialMutationPromoterAvailable sinceNov. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDB179-SfCut
Plasmid#218708PurposeEncodes codon optimized SfCut enzyme for bacterial expressionDepositorInsertSfCut
UseTags10xHisExpressionBacterialMutationPromoterAvailable sinceNov. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti-MCS-3AID-HA-2A-mTurquoiseBSD
Plasmid#225704PurposeEmpty backbone for lentiviral expression of AID degron tagged POI and mTurquoise fused BlasticidinRDepositorTypeEmpty backboneUseLentiviral and Synthetic BiologyTagsT2A-mTurquoiseExpressionMammalianMutationPromoterpEF1AAvailable sinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti-MCS-3AID-HA-2A-mCherryBSD
Plasmid#225700PurposeEmpty backbone for lentiviral expression of AID degron tagged POI and mCherry fused BlasticidinRDepositorTypeEmpty backboneUseLentiviral and Synthetic BiologyTagsT2A-mCherryExpressionMammalianMutationPromoterpEF1AAvailable sinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti-rTetR(SE)-VP64-2A-mCherryBSD
Plasmid#225695PurposeLentiviral expression of rTetR(SE) fused to VP64 and mCherry fused BlasticidinRDepositorInsertrTetR-VP64
UseLentiviral and Synthetic BiologyTags3xFLAG and T2A-mCherryExpressionMammalianMutationPromoterAvailable sinceOct. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti-rTetR(SE)-KRAB-2A-mTurquoise
Plasmid#225690PurposeLentiviral expression of rTetR(SE) fused to ZNF10 KRAB and mTurquoise-NLSDepositorInsertrTetR-ZNF10 KRAB
UseLentiviral and Synthetic BiologyTags3xFLAG and T2A-mTurquoiseExpressionMammalianMutationPromoterAvailable sinceOct. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDGB3_o1_LIR-2nd intron-3'eGFP-T35S-SIR-p35S-5'eGFP-1st intron-LIR (GB5177)
Plasmid#225442PurposeBeYDV LIR:2nd intron + 3'eGFP +T35S:BeYDV SIR + p35S + 5'eGFP + 1st intron:BeYDV LIR for INPACT configuration.DepositorInsertLIR:2nd intron:3'eGFP:T35S:SIR:p35S:5'eGFP:1st intron:LIR
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceOct. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLXB3o1_hygR-MAR10-New BeYDV geminino-SP-nano72VHH-IgG1-MAR10 (GB4954)
Plasmid#225441PurposeModule for stable transformation of Geminino 1.0-n72_h_HC flanked by two MAR10 insulators and next to a Hygromycin resistance cassette.DepositorInserthygR:MAR10:New BeYDV geminino:SP-nano72VHH-IgG1:MAR10
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceOct. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSN667
Plasmid#219963PurposeExpressed sfGFP fused human KIF1Bß(1-721) in insect cellsDepositorInsertcodon optimized human KIF1Bß (1770a.a. isoform) (KIF1B Human)
UseTagssfGFP::2xStrepII tagExpressionInsectMutationencodes 1-721PromoterpolyhedrinAvailable sinceAug. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTipi2.1_Anti-EE [IPI-3D5EE_48.K] Mouse/Rabbit LC kappa
Plasmid#221352PurposeMammalian expression of Anti-EE [IPI-3D5EE_48.K] Mouse/Rabbit LC kappa; to be used with Anti-EE [IPI-3D5EE_48.K] heavy chain (Plasmid 221351) to make the antibody.DepositorInsertAnti-EE [IPI-3D5EE_48.K] Mouse/Rabbit LC kappa
UseAffinity Reagent/ AntibodyTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 1, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pTipi2.1_Anti-GCN4 [IPI-C11L34] Mouse/Rabbit HC
Plasmid#221353PurposeMammalian expression of Anti-GCN4 [IPI-C11L34] Mouse/Rabbit HC; to be used with Anti-GCN4 [IPI-C11L34] light chain (Plasmid 221354) to make the antibody.DepositorInsertAnti-GCN4 [IPI-C11L34] Mouse/Rabbit HC
UseAffinity Reagent/ AntibodyTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 1, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pTipi2.1_Anti-EE [IPI-3D5EE_48.K] Mouse/Rabbit HC
Plasmid#221351PurposeMammalian expression of Anti-EE [IPI-3D5EE_48.K] Mouse/Rabbit HC; to be used with Anti-EE [IPI-3D5EE_48.K] light chain (Plasmid 221352) to make the antibody.DepositorInsertAnti-EE [IPI-3D5EE_48.K] Mouse/Rabbit HC
UseAffinity Reagent/ AntibodyTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 1, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
MARS-nGFP
Plasmid#205234PurposeExpresses PLEKHA5 aa 143-271 (K163A and R164A) fused to a HA-tagged GFP nanobody in mammalian cellsDepositorInsertPLEKHA5 aa 143-271 (K163A and R164A) fused to a HA-tagged GFP nanobody (PLEKHA5 Human)
UseTagsHA, Nuclear Export Sequence, mScarlet-i, and nGFP…ExpressionMammalianMutationamino acids 143-271 of PLEKHA5 (GenBank reference…PromoterCMVAvailable sinceJuly 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3-2xMARS-PLD
Plasmid#205237PurposeExpresses two repeats of PLEKAH5 aa 143-271 (K163A and R164A) fused to phospholipase D from Streptomyces sp. PMFDepositorInsertsUseTagsNuclear Export Sequence and mCherryExpressionMammalianMutationamino acids 143-271 of PLEKHA5 (GenBank reference…PromoterCMVAvailable sinceJuly 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSN668
Plasmid#219962PurposeExpressed sfGFP fused human KIF1Bß (1770 a.a. isoform) in insect cellsDepositorInsertcodon optimized human KIF1Bß (1770a.a. isoform) (KIF1B Human)
UseTagssfGFP::2xStrepII tagExpressionInsectMutationPromoterpolyhedrinAvailable sinceJuly 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLV-SFFV-ZFYVE27-WPRE-UbC-Emerald
Plasmid#214469PurposeLentiviral vector plasmid expressing human zinc finger FYVE-type containing 27 (ZFYVE27) under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoterDepositorInsertsUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJuly 11, 2024AvailabilityAcademic Institutions and Nonprofits only