We narrowed to 31,528 results for: REP

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pKLV2-U6gRNA5(gBFP)-PGKmCherry2ABFP-W

Plasmid

#67986

Purpose

Cas9 activity reporter with mCherry and BFP

Depositor

Kosuke Yusa

Article

Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

Inserts

U6gRNA cassette, PGKmCherryABFP cassette, WPRE
Guide RNA targeting modified BFP

Use

CRISPR and Lentiviral

Mutation

Deleted BbsI site within WPRE, modified BFP

Available Since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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GFP-Lamin-A

Plasmid

#206027

Purpose

Expresses GFP-tagged Lamin A in mammalian cells

Depositor

Geert van den Bogaart

Article

Warner et al Cell Rep. 2024 Feb 27;43(3):113866. doi:

Insert

Lamin-A (LMNA Human)

Tags

eGFP

Expression

Mammalian

Promoter

CMV

Available Since

March 4, 2024

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(Empty)-PGKmCherry2ABFP-W

Plasmid

#67985

Purpose

Cas9 activity reporter (control) with mCherry and BFP

Depositor

Kosuke Yusa

Article

Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

Insert

U6gRNA cassette, PGKmCherryABFP cassette, WPRE

Use

CRISPR and Lentiviral

Mutation

Deleted BbsI site within WPRE, modified BFP

Available Since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(gGFP)-PGKmCherry2AGFP-W

Plasmid

#67982

Purpose

Cas9 activity reporter with mCherry and GFP

Depositor

Kosuke Yusa

Article

Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

Insert

U6gRNA cassette, PGKmCherry2AGFP cassette, WPRE

Use

CRISPR and Lentiviral

Mutation

Deleted BbsI site within WPRE

Available Since

Nov. 10, 2015

Availability

Academic Institutions and Nonprofits only

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pKLV2.2-mU6gRNA5(SapI)-hU6gRNA5(BbsI)-PGKpuroBFP-W

Plasmid

#72667

Purpose

Lentiviral dual CRISPR gRNA expression vector (mouse U6 and human U6 promoters)

Depositor

Kosuke Yusa

Article

Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

Insert

mU6gRNA cassette, hU6gRNA cassette, PGKpuroBFP cassette, WPRE

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Oct. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(BbsI)-PGKpuro2AmCherry-W

Plasmid

#67977

Purpose

CRISPR gRNA expression vector with an improved scaffold and puro/mCherry markers

Depositor

Kosuke Yusa

Article

Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

Insert

U6gRNA cassette, PGKpuro2AmCherry cassette, WPRE

Use

Lentiviral

Mutation

Deleted BbsI site within WPRE

Promoter

human U6 promoter, mouse PGK promoter

Available Since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(BbsI)-PGKpuro2ABFP-W

Plasmid

#67974

Purpose

CRISPR gRNA expression vector with an improved scaffold and puro/BFP markers

Depositor

Kosuke Yusa

Article

Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

Insert

U6gRNA cassette, PGKpuro2ABFP cassette, WPRE

Use

Lentiviral

Mutation

Deleted BbsI site within WPRE

Promoter

human U6 promoter, mouse PGK promoter

Available Since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-GSDMD_1-275

Plasmid

#218230

Purpose

Gasdermin-D_1-275 is a pore-forming GSDMD formed by cleavage by host caspases. Transfection in HEK293 cells showed GSDMD 1-275 pores secrete 3CLpro, nucleocapsid; in excess, kills cells by pyroptosis

Depositor

Christopher Overall

Article

Grin et al Cell Rep. 2024 Dec 24;43(12):115080. doi

Insert

GSDMD (GSDMD Human)

Tags

flag

Expression

Mammalian

Mutation

construct only codes for amino acids 1-275

Available Since

July 1, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pKLV2-EF1a-Cas9Bsd-W

Plasmid

#68343

Purpose

Lentiviral vector expressing Cas9 fused with the Blasticidin resistant gene at the C-terminus

Depositor

Kosuke Yusa

Article

Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

Insert

EF1a-Cas9 cassette, WPRE

Use

CRISPR and Lentiviral

Tags

Cas9 fused with Flag-tag and a NLS at the N termi…

Promoter

EF1a

Available Since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(Empty)-PGKBFP2AGFP-W

Plasmid

#67979

Purpose

Cas9 activity reporter (control) with BFP and GFP.

Depositor

Kosuke Yusa

Article

Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

Insert

U6gRNA cassette, PGKBFP2AGFP cassette, WPRE

Use

Lentiviral

Mutation

Deleted BbsI site within WPRE

Promoter

human U6 promoter, mouse PGK promoter

Available Since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pCG_SARS-CoV2 ORF3a C-V5-tag

Plasmid

#179980

Purpose

Mammalian expression vector for SARS-CoV-2 ORF3a, V5-tagged. Sequence codon-optimized.

Depositor

Konstantin Sparrer

Article

Hayn et al Cell Rep. 2021 May 18;35(7):109126. doi:

Insert

SARS-CoV-2 ORF3a (ORF3a Synthetic)

Expression

Mammalian

Mutation

human codon-optimized

Available Since

Feb. 7, 2022

Availability

Academic Institutions and Nonprofits only

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Lenti-CMV-MLH1dn-IRES-puroR

Plasmid

#207354

Purpose

Lentiviral transfer plasmid containing CMV-driven expression cassette encoding MLH1dn for prime editing (PE4 and PE5 systems).

Depositor

Marianne Carlon

Article

Bulcaen et al Cell Rep Med. 2024 May 21;5(5):101544. d

Insert

MutL homolog 1 (MLH1 Human)

Use

Lentiviral

Mutation

Δ754-756 dominant negative mutant

Available Since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(Empty)-PGKmCherry2AGFP-W

Plasmid

#67981

Purpose

Cas9 activity reporter (control) with mCherry and GFP

Depositor

Kosuke Yusa

Article

Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

Insert

U6gRNA cassette, PGKmCherry2AGFP cassette, WPRE

Use

CRISPR and Lentiviral

Mutation

Deleted BbsI site within WPRE

Available Since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pU6-RNA-pCAG-bpNLS-miCBE-SV40NLS-bGH polyA-pCMV-mCherry-AmpR

Plasmid

#248061

Purpose

Expression vector for expression of miCBE-ωRNA v2 driven by chicken β-actin promoter and mCherry driven by CMV promoter.

Depositor

Chunlong Xu

Article

Guo et al Cell Rep. 2024 Nov 26;43(11):114973. doi

Insert

pU6-RNA-pCAG-bpNLS-miCBE-SV40NLS-bGH polyA-pCMV-mCherry-AmpR

Use

CRISPR

Tags

flag

Expression

Mammalian

Mutation

none

Promoter

CAG, U6

Available Since

Jan. 5, 2026

Availability

Academic Institutions and Nonprofits only

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PB-tetO-hNIL

Plasmid

#197089

Purpose

PiggyBac plasmid with tet-inducible expression of transcription factors for motor neuron differentiation

Depositor

Brian Wainger

Article

Held et al Cell Rep. 2023 Aug 30;42(9):113046. doi:

Insert

NGN2-P2A-ISL1-T2A-LHX3 (NEUROG2, ISL1, LHX3 Human)

Expression

Mammalian

Promoter

TRE3G

Available Since

Nov. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pKLV2-EF1a-BsdCas9-W

Plasmid

#67978

Purpose

Lentiviral vector expressing Cas9 fused with the Blasticidin resistant gene at the N-terminus

Depositor

Kosuke Yusa

Article

Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

Insert

EF1a-Cas9 cassette, WPRE

Use

CRISPR and Lentiviral

Tags

Cas9 fused with Flag-tag and a NLS at the N termi…

Promoter

EF1a

Available Since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pKLV2.2-h7SKgRNA5(SapI)-hU6gRNA5(BbsI)-PGKpuroBFP-W

Plasmid

#72666

Purpose

Lentiviral dual CRISPR gRNA expression vector (human 7SK and human U6 promoters)

Depositor

Kosuke Yusa

Article

Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

Insert

h7SKgRNA cassette, hU6gRNA cassette, PGKpuroBFP cassette, WPRE

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Oct. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pCF806-shABC2

Plasmid

#186714

Purpose

Expresses dox-controlled miR-E shRNAs (UT4GEPIR). Multi-miR shRNAs targeting A-RAF, B-RAF, and C-RAF (shABC2 = shARAF.169-shBRAF.1296-shCRAF.387). The shABC2 set is better than shABC1.

Depositor

Christof Fellmann

Article

Amen et al Cell Rep Methods. 2022 Jun 21;2(7):10023

Insert

ARAF, BRAF, CRAF (BRAF, RAF1, ARAF Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Jan. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pYX-N-Puro-mNeon-TSC22D4

Plasmid

#222671

Purpose

endogenously tag TSC22D4 with N-terminal mNeon

Depositor

Jason Moffat

Article

Xiao et al Cell Rep. 2024 Jul 23;43(7):114417. doi:

Insert

TSC22D4 (TSC22D4 Human)

Tags

mNeon

Expression

Bacterial

Available Since

Oct. 18, 2024

Availability

Academic Institutions and Nonprofits only

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Anti-Kv7.2/KCNQ2 K+ channel [N26A/23R]

Plasmid

#177524

Purpose

Mammalian Expression Plasmid of anti-Kv7.2/KCNQ2 K+ channel (Human). Derived from hybridoma N26A/23.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-Kv7.2/KCNQ2 K+ channel (Homo sapiens) recombinant mouse monoclonal antibody (KCNQ2 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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