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pYPQ132-STU-Fn

Plasmid

#138098

Purpose

Golden Gate entry vector for 2nd crRNA cloning with FnCas12a crRNA scaffold flanked by HH and HDV ribozymes

Depositor

Yiping Qi

Publication

Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available since

March 31, 2021

Availability

Academic Institutions and Nonprofits only

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pYPQ133-STU-Fn

Plasmid

#138101

Purpose

Golden Gate entry vector for 3rd crRNA cloning with FnCas12a crRNA scaffold flanked by HH and HDV ribozymes

Depositor

Yiping Qi

Publication

Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available since

March 31, 2021

Availability

Academic Institutions and Nonprofits only

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pIR3DH8K

Plasmid

#165069

Purpose

Knocking out GAL80 using KanMX4 marker

Depositor

Claudia Vickers

Publication

Lu et al Nat Commun. 2021 Feb 16;12(1):1051. doi:

Insert

gal80Arm1-P-TPI1-KanMX4-gal80Arm2

Expression

Yeast

Mutation

WT

Available since

March 22, 2021

Availability

Academic Institutions and Nonprofits only

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ShaCas9

Plasmid

#163794

Purpose

Expresses ShaCas9, and cloning backbone for sgRNA

Depositor

Yongming Wang

Publication

Hu et al Nucleic Acids Res. 2021 Mar 15. pii: 617

Type

Empty backbone

Use

AAV and CRISPR

Expression

Mammalian

Available since

March 18, 2021

Availability

Academic Institutions and Nonprofits only

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pTFPhiC31

Plasmid

#139671

Purpose

Expression cassette for phiC31 Integrase in T-DNA vector

Depositor

James Birchler

Publication

Cody et al Plant Direct. 2020 Mar 9;4(3):e00209. do

Insert

phiC31 Integrase

Expression

Plant

Promoter

Maize Ubiquitin

Available since

Aug. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pC0.203

Plasmid

#119604

Purpose

Level 0 part. TSS promoter

Depositor

Alistair McCormick

Publication

Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

Insert

J23103_TSS

Use

Synthetic Biology

Available since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pC0.083

Plasmid

#119592

Purpose

Level 0 part. Promoter

Depositor

Alistair McCormick

Publication

Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

Insert

J23119MH_V01

Use

Synthetic Biology

Available since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pC.059

Plasmid

#119564

Purpose

Level 0 part. Promoter

Depositor

Alistair McCormick

Publication

Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

Insert

Ptrc10

Use

Synthetic Biology

Available since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pWS627

Plasmid

#123064

Purpose

G___ bicistronic protein

Depositor

Tom Ellis

Publication

Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

Insert

Ste4-2A-Ste18 [3-4a]

Expression

Yeast

Available since

April 5, 2019

Availability

Academic Institutions and Nonprofits only

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BB1_12_YlpTEF

Plasmid

#117819

Purpose

Promoter pTEF of Y. lipolytica for GoldenMOCS

Depositor

Michael Sauer

Publication

Egermeier et al FEMS Microbiol Lett. 2019 Jan 29. pii: 5

Insert

YlpTEF

Expression

Bacterial

Available since

Feb. 13, 2019

Availability

Academic Institutions and Nonprofits only

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BB1_34_YlCyc1TT

Plasmid

#117821

Purpose

Transcription terminator Cyc1TT of Y. lipolytica for GoldenMOCS

Depositor

Michael Sauer

Publication

Egermeier et al FEMS Microbiol Lett. 2019 Jan 29. pii: 5

Insert

YlCyc1TT

Expression

Bacterial

Available since

Feb. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pMEG_BB3_YL68N_AE

Plasmid

#117832

Purpose

GoldenMOCS backbone for 4 TU in Y. lipolytica

Depositor

Michael Sauer

Publication

Egermeier et al FEMS Microbiol Lett. 2019 Jan 29. pii: 5

Type

Empty backbone

Expression

Bacterial and Yeast

Available since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pMEG_BB3_YL68N_AC

Plasmid

#117830

Purpose

GoldenMOCS backbone for 2 TU in Y. lipolytica

Depositor

Michael Sauer

Publication

Egermeier et al FEMS Microbiol Lett. 2019 Jan 29. pii: 5

Type

Empty backbone

Expression

Bacterial and Yeast

Available since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pMEG_BB3_YL68N_AB

Plasmid

#117829

Purpose

GoldenMOCS backbone for 1 TU in Y. lipolytica

Depositor

Michael Sauer

Publication

Egermeier et al FEMS Microbiol Lett. 2019 Jan 29. pii: 5

Type

Empty backbone

Expression

Bacterial and Yeast

Available since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pMEG_BB3_YL68N_AD

Plasmid

#117831

Purpose

GoldenMOCS backbone for 3 TU in Y. lipolytica

Depositor

Michael Sauer

Publication

Egermeier et al FEMS Microbiol Lett. 2019 Jan 29. pii: 5

Type

Empty backbone

Expression

Bacterial and Yeast

Available since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pBC2102-reci

Plasmid

#117171

Purpose

U6-sgRNA-EFS-Cas9-T2A-mCherry-P2A-Hygro; wtCas9 without NLS

Depositor

David Savage

Publication

Oakes et al Nat Biotechnol. 2016 May 2. doi: 10.1038

Insert

reci_wtCas9 without NLS

Use

Lentiviral

Promoter

U6 Promoter

Available since

Oct. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pDM1019

Plasmid

#108976

Purpose

shuttle vector, N-terminal GFP tagging

Depositor

Rob Kay

Publication

Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

Type

Empty backbone

Use

Dictyostelium discoideum shuttle vector

Tags

GFP

Available since

July 18, 2018

Availability

Academic Institutions and Nonprofits only

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pMOD_A1310

Plasmid

#91034

Purpose

Module A, Promoter: ZmUbi, Gene: TaCas9_H840A nickase, Terminator: HSP

Depositor

Daniel Voytas

Publication

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

TaCas9_H840A nickase

Use

CRISPR

Mutation

H840A

Promoter

ZmUbi

Available since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

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pMOD_A0208

Plasmid

#91004

Purpose

Module A, Promoter: EC1.2, Gene: AtCas9_D10A nickase, Terminator: HSP

Depositor

Daniel Voytas

Publication

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

AtCas9_D10A nickase

Use

CRISPR

Mutation

D10A

Promoter

EC1.2

Available since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

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pFUSB4_AGGG

Plasmid

#48410

Depositor

Stephen Ekker

Publication

Ma et al PLoS One. 2013 May 29;8(5):e65259. doi:

Insert

RVD sequence: NI NN NN NN

Use

TALEN

Expression

Mammalian

Available since

March 14, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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