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pBad-LgBiT-PhoCl1-SmBiT-MBP

Plasmid

#164034

Purpose

luciferase-based PhoCl screening construct

Depositor

Robert Campbell

Publication

Lu et al Chem Sci. 2021 May 31;12(28):9658-9672.

Insert

LgBiT-PhoCl1-SmBiT-MBP

Tags

His tag, T7 tag, Xpress Tag

Expression

Bacterial

Promoter

araBad promotor

Available since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pUC19_CRISPR_DpmrA

Plasmid

#160903

Purpose

CRISPR_Cas9, lambda red recombineering

Depositor

Anne-Catrin Uhlemann

Publication

McConville et al Cell Rep. 2020 Oct 27;33(4):108313. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Inserts

Cas9
Lambda Red Recombineering Genes
Zeocin Resistance Cassette

Promoter

araBAD

Available since

Feb. 9, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pB-EF1a-NLuc-IRES-Puro

Plasmid

#130936

Purpose

PiggyBac vector for constitutive NanoLuc expression in mammalian cells

Depositor

James Thomson

Publication

Chu et al Cell Rep. 2019 Aug 27;28(9):2247-2255.e5

Insert

NanoLuc

Expression

Mammalian

Promoter

EF1a

Available since

Oct. 11, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pREDIA

Plasmid

#51625

Purpose

E. coli genome modification

Depositor

Sheng Yang

Publication

Yang et al Appl Environ Microbiol. 2014 Jul 1;80(13

Insert

I-SceI (SCEI Budding Yeast)

Use

Red recombinase and i-scei endonuclease

Expression

Bacterial

Promoter

pRha

Available since

May 22, 2015

Availability

Academic Institutions and Nonprofits only

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pET-(-30)GFP-9xGGS-Cre-6xHis

Plasmid

#62372

Purpose

Expression of (-30)GFP-9xGGS-Cre-6xHis in bacterial cells

Depositor

David Liu

Publication

Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

Insert

(-30)GFP-9xGGS-Cre-6xHis

Tags

6xHis tag and (-30)GFP

Expression

Bacterial

Promoter

T7

Available since

May 11, 2015

Availability

Academic Institutions and Nonprofits only

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CMV-CAR-GECO1

Plasmid

#45493

Purpose

Carmine intensiometric genetically encoded Ca2+-indicators for optical imaging

Depositor

Robert Campbell

Publication

Wu et al ACS Chem Neurosci. 2013 Mar 19.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

CAR-GECO1

Expression

Mammalian

Mutation

R-GECO1 E163V/I166V/V174T/M176I/F222I/A302P

Promoter

CMV

Available since

July 23, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pLJM60-Tia1

Plasmid

#38243

Depositor

David Sabatini

Publication

Thoreen et al Nature. 2012 May 2;485(7396):109-13. doi

Insert

Tia1 (Tia1 Mouse)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available since

Nov. 7, 2012

Availability

Academic Institutions and Nonprofits only

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pET His6 MBP Asn10 TEV LIC cloning vector (1C)

Plasmid

#29654

Depositor

Scott Gradia

Type

Empty backbone

Tags

6xHis, 10xAsn, MBP, and TEV cleavage site

Expression

Bacterial

Promoter

T7-lacO (lactose/IPTG inducible)

Available since

Aug. 17, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

15 Articles

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GFP-C1-CAMKIIalpha-T286A

Plasmid

#21220

Depositor

Tobias Meyer

Publication

Shen et al Science. 1999 Apr 2. 284(5411):162-6.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

CAMKIIalpha-T286A (Camk2a Rat)

Tags

GFP

Expression

Mammalian

Mutation

T286A (Lab plasmid KS 0003).

Available since

July 29, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pGIPz-mPD-L1

Plasmid

#121488

Purpose

Expression of mouse PD-L1 WT

Depositor

Mien-Chie Hung

Publication

Li et al Nat Commun. 2016 Aug 30;7:12632. doi: 10

Insert

PD-L1

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available since

Feb. 2, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pET251-pUC 12xMS2V6 Loxp KANr Loxp

Plasmid

#104392

Purpose

Yeast endogenous mRNA tagging

Depositor

Robert Singer, Evelina Tutucci

Publication

Tutucci et al Nat Methods. 2017 Nov 13. doi: 10.1038/n

Insert

12xMS2V6

Expression

Bacterial

Mutation

All Loops are U-variants, interspaced by 50 nucle…

Available since

Jan. 2, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pEGFP-N1-hAIM2

Plasmid

#51542

Purpose

Express GFP-tagged hAIM2 in mammalian cells

Depositor

Emad Alnemri

Publication

Fernandes-Alnemri et al Nature. 2009 Mar 26;458(7237):509-13. do

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

AIM2 (AIM2 Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available since

March 19, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pFA6a-3HA-kanMX6

Plasmid

#39295

Depositor

Jurg Bahler, John Pringle

Publication

Bahler et al Yeast. 1998 Jul;14(10):943-51.

Insert

KanR

Use

Yeast genomic targeting

Tags

3HA, A. gossypii translation elongation factor 1a…

Available since

Sept. 25, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pEGFPpA-ACN

Plasmid

#20343

Depositor

Mario Capecchi

Publication

Wu et al Nat Protoc. 2008 . 3(6):1056-76.

Insert

EGFPpA-ACN

Use

Cre/Lox and Mouse Targeting

Expression

Mammalian

Available since

March 11, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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Agrp-pGL3

Plasmid

#17552

Depositor

Domenico Accili

Publication

Kitamura et al Nat Med. 2006 May . 12(5):534-40.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Agrp (Agrp Mouse)

Use

Luciferase

Mutation

Contains ~400bp of the mouse Pomc gene promoter, …

Available since

Oct. 15, 2008

Availability

Academic Institutions and Nonprofits only

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pGL3-FLIP1500

Plasmid

#16016

Depositor

Wafik El-Deiry

Publication

Ricci et al Mol Cell Biol. 2004 Oct . 24(19):8541-55

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

FLIP (CFLAR Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Available since

Nov. 2, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles
PB-TO-NFIA.1-SOX9 (bi-directional promoter)

Plasmid

#182306

Purpose

Piggybac Tet-ON plasmid for differentiating hiPSCs into astrocytes via NFIA and SOX9 expression

Depositor

iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

Inserts

NFIA (NFIA Human)
SOX9 (SOX9 Human)
puroR-T2A-mycNLS-mTagBFP2

Use

Piggybac

Tags

None and T2A-mycNLS-mTagBFP2

Expression

Mammalian

Promoter

TRE3G-Bi-directional

Available since

April 14, 2022

Availability

Academic Institutions and Nonprofits only

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pDEST-HemmarG

Plasmid

#31221

Depositor

Yuh-Nung Jan

Publication

Han et al Proc Natl Acad Sci U S A. 2011 Jun 7;108

Insert

CD4-tdGFP (CD4 Fly, Human, Aequorea victoria)

Tags

CD4 and EGFP/GFP

Expression

Insect

Mutation

Fusion of signal peptide of Drosophila Akh gene, …

Available since

Oct. 27, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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ACE2

Plasmid

#164219

Purpose

Expresses full length human ACE2 protein, along with TagBFP reporter and Puromycin selection marker

Depositor

Sriram Neelamegham

Publication

Yang et al Elife. 2020 Oct 26;9. pii: 61552. doi: 1

Insert

ACE2 (ACE2 Human)

Use

Lentiviral

Mutation

silent mutation to remove internal EcoRI site (GA…

Promoter

CMV

Available since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pET28a-6XHis-SUMO-SIRT2

Plasmid

#102622

Purpose

Expresses a truncation (aa38-356) of human SIRT2 isoform 1 in bacteria for purification

Depositor

Hening Lin

Publication

Jing et al Cancer Cell. 2016 Mar 14;29(3):297-310.

Insert

SIRT2 (SIRT2 Human)

Tags

6XHis-SUMO

Expression

Bacterial

Mutation

Deleted amino acids 1-37 and 357-389

Available since

Feb. 1, 2018

Availability

Academic Institutions and Nonprofits only

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