We narrowed to 3,099 results for: GAI

1. pBXNH3LCA_TM287/288

   Plasmid

   #132701

   Purpose

   expresses TM287/288 in E.coli, to use as positive control in ELISA

   Depositor

   Markus Seeger

   Article

   Zimmermann et al Nat Protoc. 2020 Apr 8. pii: 10.1038/s41

   Insert

   TM287/288 (TM0288, TM0287 Thermotoga maritima)
   

   Use

   Tags

   Avi and His

   Expression

   Bacterial

   Mutation

   Promoter

   pBAD

   Available since

   Oct. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pX459-sg-tdTomato90/816

   Plasmid

   #179918

   Purpose

   Double cutting Cas9 plasmid with puromycin resistance and a single guide RNA targeting tdTomato fluorescent protein sites 90 and 816.

   Depositor

   Indeever Madireddy, Johan Sosa

   Article

   Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

   Insert

   tdTomato sgRNA
   

   Use

   CRISPR

   Tags

   2A-Puro and 3XFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   April 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCold I-Hero9

   Plasmid

   #187927

   Purpose

   Bacterial expression of heat-resistant obscure (Hero) protein Hero9

   Depositor

   Yukihide Tomari

   Article

   Tsuboyama et al PLoS Biol. 2020 Mar 12;18(3):e3000632. d

   Insert

   Hero9
   

   Use

   Tags

   Flag-6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. tdEos-Tubulin-C-18

   Plasmid

   #57683

   Purpose

   Localization: Microtubules, Excitation: 505 / 569, Emission: 516 / 581

   Depositor

   Michael Davidson

   Article

   Solowska et al J Neurosci. 2014 Jan 29;34(5):1856-67. d

   Insert

   Tubulin (TUBA1B Human)
   

   Use

   Tags

   tdEos

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 13, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

5. pJBL704

   Plasmid

   #140374

   Purpose

   Expresses TetR-regulated 3WJdB RNA aptamer driven by T7 promoter with the tetO sequence 4BP dowsntream from the promoter

   Depositor

   Julius Lucks

   Article

   Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038

   Insert

   T7-4BP-tetO-3WJdB-T
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pLKO.1-Teton-puro-shFAK #1

   Plasmid

   #198757

   Purpose

   conditional knockdown of FAK

   Depositor

   Hyonchol Jang

   Article

   Kim et al Cancers (Basel). 2019 Sep 2;11(9). pii:

   Insert

   shFAK #1 (PTK2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pLKO.1-Teton-puro-shFAK #2

   Plasmid

   #198758

   Purpose

   conditional knockdown of FAK

   Depositor

   Hyonchol Jang

   Article

   Kim et al Cancers (Basel). 2019 Sep 2;11(9). pii:

   Insert

   shFAK #2 (PTK2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pGL3-RbE2Fmut

   Plasmid

   #32391

   Depositor

   Julien Sage

   Article

   Burkhart et al Mol Cell Biol. 2010 Apr;30(7):1729-45. E

   Insert

   Rb promoter (Rb1 Mouse)
   

   Use

   Luciferase

   Tags

   Luciferase

   Expression

   Mammalian

   Mutation

   Mutated E2F binding site: TTTCCCGC --> TTTACATC

   Promoter

   Available since

   Sept. 27, 2011

   Availability

   Academic Institutions and Nonprofits only

9. pBZ191-pU6-sgBFP-CBh-sv40NLS-Cas9-NLS-T2A-mCherry

   Plasmid

   #170183

   Purpose

   Expression vector for a sgRNA against BFP and spCas9 linked to mCherry via a T2A peptide

   Depositor

   Hunter Fraser

   Article

   Zhao et al CRISPR J. 2022 Jan 24. doi: 10.1089/cris

   Insert

   sgBFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   Jan. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pJBL705

    Plasmid

    #140375

    Purpose

    Expresses TetR-regulated 3WJdB RNA aptamer driven by T7 promoter with the tetO sequence 5BP dowsntream from the promoter

    Depositor

    Julius Lucks

    Article

    Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038

    Insert

    T7-5BP-tetO-3WJdB-T
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pET21a(+) LdtMt2 (131-408)

    Plasmid

    #59337

    Purpose

    Expresses LdtMt2 for L,D-transpeptidation

    Depositor

    SeWon Suh

    Article

    Kim et al Acta Crystallogr D Biol Crystallogr. 201

    Insert

    LdtMt2 (131-408)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

12. NLS-dCas9-HA-NLS-GFP4-VPR

    Plasmid

    #183924

    Purpose

    VPR activation domain coupled to catalytically inactive dCas9 for localization; contains a GFP tetramer fpr labeling and as a spacer

    Depositor

    Karsten Rippe

    Article

    Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

    Insert

    dCas9-GFP4-VPR
    

    Use

    CRISPR

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available since

    July 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCNL-N1_zyxin

    Plasmid

    #65682

    Purpose

    Expresses Zyxin-CNL in mammalian cells

    Depositor

    Yasushi Okada

    Article

    Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

    Insert

    zyxin (Zyx Mouse)
    

    Use

    Luciferase

    Tags

    CNL

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 17, 2015

    Availability

    Academic Institutions and Nonprofits only

14. pTorPE-GEX-GECO1

    Plasmid

    #32464

    Depositor

    Robert Campbell

    Article

    Zhao et al Science. 2011 Sep 30;333(6051):1888-91.

    Insert

    GEX-GECO1.0
    

    Use

    Lab constructed

    Tags

    6 His tag and Modified TorA MNNNDLFQASRRRFLAQLG[G…

    Expression

    Bacterial

    Mutation

    GCaMP3 K69E/K119I/L173Q/T223S/F315L/F368L/L372Q/K…

    Promoter

    Available since

    March 30, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCNL-N1_Mito

    Plasmid

    #65670

    Purpose

    Expresses Mito-CNL in mammalian cells

    Depositor

    Yasushi Okada

    Article

    Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

    Insert

    cytochrome c oxidase subunit VIII
    

    Use

    Luciferase

    Tags

    CNL

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLPC myc-mRAP1 I312R

    Plasmid

    #73137

    Purpose

    Expression of myc-tagged mRAP1 with single point mutatoin I312R

    Depositor

    Agnel Sfeir

    Article

    Yeung et al Cell Rep. 2013 Jun 27;3(6):1847-56. doi:

    Insert

    Rap1 (Terf2ip Mouse)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    isoleucine changed to an arginine at amino acid 3…

    Promoter

    Available since

    March 11, 2016

    Availability

    Academic Institutions and Nonprofits only

17. pCNL-C1_RhoB

    Plasmid

    #65697

    Purpose

    Expresses CNL-RhoB in mammalian cells

    Depositor

    Yasushi Okada

    Article

    Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

    Insert

    RhoB (RHOB Human)
    

    Use

    Luciferase

    Tags

    CNL

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

18. pONL-N1_Golgi

    Plasmid

    #65695

    Purpose

    Expresses Golgi-ONL in mammalian cells

    Depositor

    Yasushi Okada

    Article

    Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

    Insert

    N-terminal 81 amino acids of beta 1,4-galactosyltransferase
    

    Use

    Luciferase

    Tags

    ONL

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pGEX-Elafin

    Plasmid

    #18099

    Depositor

    Ronny Drapkin

    Article

    Clauss et al Neoplasia. 2010 Feb;12(2):161-72.

    Insert

    Elafin (PI3 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 11, 2008

    Availability

    Academic Institutions and Nonprofits only

20. pCNL-N1_ITPKA

    Plasmid

    #65688

    Purpose

    Expresses ITPKA-CNL in mammalian cells

    Depositor

    Yasushi Okada

    Article

    Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

    Insert

    inositol triphosphate 3-kinase A
    

    Use

    Luciferase

    Tags

    CNL

    Expression

    Mammalian

    Mutation

    contains residues 9-52

    Promoter

    CMV

    Available since

    June 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge