We narrowed to 25,140 results for: Nes

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pANC98c(3xFLAG-NES-FRB-(G4S)4-cTEV(219)-IRES-EBFP)

Plasmid

#248359

Purpose

Lentiviral vector that can express FRB along with a truncated C terminal split TEV. Expressed off of CMV tet ON promoter with EBFP marker

Depositor

Arnab Mukherjee

Article

Chacko et al Sci Adv. 2026 Jan 16;12(3):eaec1211. doi

Insert

3xFLAG-NES-FRB-(G4S)4x-cTEV(219)

Use

Lentiviral

Promoter

tight TRE

Available Since

Jan. 30, 2026

Availability

Academic Institutions and Nonprofits only

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pLV hU6-VEGFR2#3 sgRNA Nestin-dCas9-KRAB-T2a-GFP

Plasmid

#196990

Purpose

Derived from pLV hU6-sgRNA Nestin-dCas9-KRAB-T2a-GFP with KDR sgRNA

Depositor

Elizabeth Kirby

Article

Dause et al Mol Neurobiol. 2025 Mar 25. doi: 10.1007

Insert

VEGFR2

Use

CRISPR and Lentiviral

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-CMV-Puro-P2A-NES/PKI-mNeonGreen2x-NLS/FUS

Plasmid

#228400

Purpose

Encodes NES/PKI-mNeonGreen2x-NLS/FUS which is a tandem of mNeongreen with an N-terminal PKI NES and a C-terminal FUS NLS that shuttles between the nucleus and the cytoplasm

Depositor

Dirk Daelemans

Article

Vercruysse et al Commun Biol. 2024 Nov 23;7(1):1560. doi:

Insert

NES/PKI-mNeonGreen2x-NLS/FUS

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pGL4-CMV-NLS/cMyc-AcGFP2x-NES/iKB2-P2A-Puro

Plasmid

#228398

Purpose

Encodes NLS/cMyc-AcGFP2x-NES/iKB2 which is a tandem of AcGFP with an N-terminal cMyc NLS and a C-terminal iKB2 NES that shuttles between the nucleus and the cytoplasm

Depositor

Dirk Daelemans

Article

Vercruysse et al Commun Biol. 2024 Nov 23;7(1):1560. doi:

Insert

NLS/cMyc-AcGFP2x-NES/iKB2

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-miRFP680-NES-p2A-ezrin-T567A-mRuby3-IRES-Neo

Plasmid

#222637

Purpose

Ezrin activation reporter, T567 phosphorylation deficient

Depositor

Arnold Hayer

Article

Marshall-Burghardt et al Sci Adv. 2024 Sep 6;10(36):eadn6858. doi

Insert

miRFP680-NES-p2A-ezrin-T567A-mRuby3 (EZR Human)

Use

Lentiviral

Tags

mRuby3

Mutation

T567A

Promoter

EF1a

Available Since

July 23, 2024

Availability

Academic Institutions and Nonprofits only

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pLV-EF1a-NLS-msfGFP-T2A-mCherry-NES> mPGK-PuroR

Plasmid

#218916

Purpose

Lentiviral construct expressing msfGFP and nuclear mCherry proteins.

Depositor

Sven Eyckerman

Article

Delhaye et al Cell Rep Methods. 2024 Jul 15;4(7):10081

Insert

EF1a-msfGFP-T2A-mCherry-NLS > mPGK-PuroR

Use

Lentiviral

Available Since

June 4, 2024

Availability

Academic Institutions and Nonprofits only

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pJT216 Lenti pEF-dCas9-3XFLAG-LibCloneSite-T2A-tagBFP-P2A-Blast

Plasmid

#187320

Purpose

dCas9 expression vector with cloning site for C terminal fusions

Depositor

Michael Bassik, Lacra Bintu

Article

Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

3XFLAG

Expression

Mammalian

Available Since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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10xHis-MBP-TEV-S. pyogenes dCas9 M1C D10A C80S H840A C574S

Plasmid

#60815

Purpose

dCas9 with single cysteine residue (M1C) for site-specific labelling

Depositor

Jennifer Doudna

Article

O'Connell et al Nature. 2014 Sep 28. doi: 10.1038/nature

Insert

dCas9 M1C D10A C80S H840A C574S

Tags

His10 and MBP

Expression

Bacterial

Mutation

M1C D10A C80S H840A C574S

Available Since

Dec. 4, 2014

Availability

Academic Institutions and Nonprofits only

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plx208-CMV-Signal seuqence-enkephalin-CapC-FLAG-μOR-NNES-SmBit

Plasmid

#213538

Purpose

Expresses the CapC-caged opioid peptide in mammalian cells

Depositor

Wenjing Wang

Article

Shen et al Nat Methods. 2023 Jan;20(1):112-122. doi

Insert

Signal seuqence-enkephalin-CapC-FLAG-μOR-NNES-SmBit

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pKG3658_pPV2-NLS-FXBP-ZF43-P2A-NES-VP64-FRB-P2A-iRFP720

Plasmid

#239699

Purpose

BsaI Golden Gate assembly plasmid encoding the COMET NLS-FXBP-ZF43-P2A-NES-VP64-FRB-P2A-iRFP720 CDS

Depositor

Kate Galloway

Article

Peterman et al Nucleic Acids Research, Volume 53, Issue

Insert

NLS-FXBP-ZF43-P2A-NES-VP64-FRB-P2A-iRFP720

Use

Synthetic Biology

Available Since

July 10, 2025

Availability

Academic Institutions and Nonprofits only

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pLV hU6-Non-target sgRNA Nestin-dCas9-KRAB-T2a-GFP

Plasmid

#196989

Purpose

Derived from pLV hU6-sgRNA Nestin-dCas9-KRAB-T2a-GFP with non-target sgRNA

Depositor

Elizabeth Kirby

Article

Dause et al Mol Neurobiol. 2025 Mar 25. doi: 10.1007

Insert

Non-Target

Use

CRISPR and Lentiviral

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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pGL4-CMV-3xFLAG-NLS/SV40-AcGFP-NES/PKI-P2A-Puro

Plasmid

#228399

Purpose

Encodes 3xFLAG-NLS/SV40-AcGFP-NES/PKI which is a AcGFP with an N-terminal SV40 NLS and a C-terminal PKI NES that shuttles between the nucleus and the cytoplasm

Depositor

Dirk Daelemans

Article

Vercruysse et al Commun Biol. 2024 Nov 23;7(1):1560. doi:

Insert

3xFLAG-NLS/SV40-AcGFP-NES/PKI

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pUC57-ITR2-H1-GFP sgRNA Target 3- eCMV- S.pyoegenes-3XFLAG-SV40NLS-ITR2

Plasmid

#210739

Purpose

Coding for Sp Cas9 alongside Sp sgRNA targeting GFP Target 3

Depositor

Vincent Dion

Article

Mangin et al bioRxiv 2023.10.24.563270

Inserts

Sp Cas9 (cas9 S pyogenes)
Sp sgRNA GFP Target 3

Use

CRISPR

Tags

3xFLAG, SV40 NLS, PolyA signal

Expression

Mammalian

Promoter

H1 and eCMV

Available Since

Jan. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pUC57-ITR2-H1-GFP sgRNA Target 1- eCMV- S.pyoegenes-3XFLAG-SV40NLS-ITR2

Plasmid

#210737

Purpose

Coding for Sp Cas9 alongside Sp sgRNA targeting GFP Target 1

Depositor

Vincent Dion

Article

Mangin et al bioRxiv 2023.10.24.563270

Inserts

Sp Cas9 (cas9 S pyogenes)
Sp sgRNA GFP Target 1

Use

CRISPR

Tags

3xFLAG, SV40 NLS, PolyA signal

Expression

Mammalian

Promoter

H1 and eCMV

Available Since

Jan. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pUC57-ITR2-H1-GFP sgRNA Target 2- eCMV- S.pyoegenes-3XFLAG-SV40NLS-ITR2

Plasmid

#210738

Purpose

Coding for Sp Cas9 alongside Sp sgRNA targeting GFP Target 2

Depositor

Vincent Dion

Article

Mangin et al bioRxiv 2023.10.24.563270

Inserts

Sp Cas9 (cas9 S pyogenes)
Sp sgRNA GFP Target 2

Use

CRISPR

Tags

3xFLAG, SV40 NLS, PolyA signal

Expression

Mammalian

Promoter

H1 and eCMV

Available Since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pUC57-ITR2-H1-GFP sgRNA Target 4- eCMV- S.pyoegenes-3XFLAG-SV40NLS-ITR2

Plasmid

#210740

Purpose

Coding for Sp Cas9 alongside Sp sgRNA targeting GFP Target 4

Depositor

Vincent Dion

Article

Mangin et al bioRxiv 2023.10.24.563270

Inserts

Sp Cas9 (cas9 S pyogenes)
Sp sgRNA GFP Target 4

Use

CRISPR

Tags

3xFLAG, SV40 NLS, PolyA signal

Expression

Mammalian

Promoter

H1 and eCMV

Available Since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pJT126 lenti pEF-rTetR(SE-G72P)-3XFLAG-LibCloneSite-T2A-mCherry-BSD-WPRE

Plasmid

#161926

Purpose

Lentiviral backbone for GoldenGate cloning effectors as a fusion with rTetR(SE-G72P)-3XFLAG

Depositor

Michael Bassik, Lacra Bintu

Article

Tycko et al Cell. 2020 Dec 9. pii: S0092-8674(20)315

Insert

rTetR(SE-G72P)

Use

Lentiviral and Synthetic Biology

Tags

3XFLAG

Expression

Mammalian

Promoter

pEF

Available Since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-IE94-Flex-TM-KA2-Cam-NES-TeVN-AsLOV2-TEVseq-bGH-PA

Plasmid

#171619

Purpose

AAV floxed Soma-targeted Cal-Light with KA2

Depositor

Hyungbae Kwon

Article

Hyun et al Nat Commun. 2022 Dec 12;13(1):7692. doi:

Insert

IE94-Flex-TM-KA2-Cam-NES-TeVN-AsLOV2-TEVseq-bGH-PA

Use

AAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-gLuc sp-GS linker-HiBit-Flag-oβ2AR-TS-NNES-eLOV-TEVcs-Flag-Gal4-V5

Plasmid

#125234

Purpose

Expresses SPARK oβ2AR transmembrane component in mammalian cells

Depositor

Alice Ting

Article

Kim et al eLife. 2019 Apr 3;8. pii: 43826. doi: 10

Insert

gLuc sp-GS linker-HiBit-Flag-oβ2AR-TS-NNES-eLOV-TEVcs-Flag-Gal4-V5

Use

AAV

Tags

Flag, V5

Expression

Mammalian

Promoter

CMV

Available Since

July 11, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti_EF1α-NES-Kinprola_PKA-mEGFP_bGH-PA term_EF1α_FLAG-SV40NLS-Cas9-NLS-T2A-BSD-WPRE-SV40

Plasmid

#233371

Purpose

EF1α driven co-expression of the PKA activity recorder Kinprola_PKA fused to mEGFP and Cas9 expression through lentivirus transduction

Depositor

Kai Johnsson

Article

Sun et al Nat Chem Biol. 2025 Jul 10. doi: 10.1038

Inserts

NES-Kinprola_PKA-mEGFP
FLAG-SV40NLS-Cas9-NLS-T2A-BSD

Use

Lentiviral

Tags

FLAG, NES, and mEGFP

Promoter

EF1α

Available Since

March 27, 2025

Availability

Academic Institutions and Nonprofits only

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