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pCaSpeR dhd-eGFP

Plasmid

#113003

Purpose

P element vector for transposon insertion of dhd-eGFP into Drosophila genome

Depositor

Terry Orr-Weaver

Insert

dhd (dhd Fly)

Use

P insertion vector

Tags

eGFP

Available since

Aug. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pFastBac His HA GNU WT

Plasmid

#112994

Purpose

Baculovirus expression of His/HA-tagged Drosophila gnu

Depositor

Terry Orr-Weaver

Publication

Hara et al Elife. 2017 May 30;6. doi: 10.7554/eLife

Insert

gnu (gnu Fly)

Tags

His/HA

Expression

Insect

Available since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

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20XUAS IVS Jaws mVenus_tr

Plasmid

#111552

Purpose

Optogenetics in Drosophila: UAS-induced expression of Jaws mVenus

Depositor

Vivek Jayaraman

Publication

Chuong et al Nat Neurosci. 2014 Aug;17(8):1123-9. doi

Insert

Jaws_mVenus_tr

Expression

Insect

Mutation

wt

Promoter

hsp70

Available since

June 26, 2018

Availability

Academic Institutions and Nonprofits only

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10XUAS IVS Jaws mVenus_tr

Plasmid

#111553

Purpose

Optogenetics in Drosophila: UAS-induced expression of Jaws mVenus

Depositor

Vivek Jayaraman

Publication

Chuong et al Nat Neurosci. 2014 Aug;17(8):1123-9. doi

Insert

Jaws_mVenus_tr

Expression

Insect

Mutation

wt

Promoter

hsp70

Available since

June 26, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pUAST-HA-dAtg13

Plasmid

#111802

Purpose

Insect Expression of dAtg13

Depositor

Jun Hee Lee

Publication

Kim et al Autophagy. 2013 Aug;9(8):1201-13. doi: 1

Insert

dAtg13 (Atg13 Fly)

Tags

HA

Expression

Insect

Available since

June 21, 2018

Availability

Academic Institutions and Nonprofits only

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pJC-ase0.8kb-TALE3-VP64-P10

Plasmid

#104610

Purpose

Expresses TALE3 under the control of a 0.8kb ase enhancer element

Depositor

Tudor Fulga

Publication

Toegel et al Nat Commun. 2017 Nov 21;8(1):1663. doi:

Insert

0.8kb ase enhancer element (ase Fly)

Use

TALEN

Expression

Insect

Promoter

hsp70

Available since

Feb. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pJC-mhc2.4kb-TALE3-VP64-P10

Plasmid

#104611

Purpose

Expresses TALE3 under the control of a 2.4kb mhc enhancer element

Depositor

Tudor Fulga

Publication

Toegel et al Nat Commun. 2017 Nov 21;8(1):1663. doi:

Insert

2.4kb mhc enhancer element (Mhc Fly)

Use

TALEN

Expression

Insect

Promoter

hsp70

Available since

Jan. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX-4T-1 dSesn (1187)

Plasmid

#66919

Purpose

bacterial expression of dSesn

Depositor

Michael Karin, Jun Hee Lee

Publication

Lee et al Science. 2010 Mar 5;327(5970):1223-8. do

Insert

Sesn (Sesn Fly)

Tags

GST

Expression

Bacterial

Promoter

tac

Available since

Dec. 18, 2017

Availability

Academic Institutions and Nonprofits only

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pMCh-56

Plasmid

#92260

Purpose

Plasmid for expression of HA-tagged Drosophila melanogaster GW182 in D. melanogaster cells

Depositor

Marina Chekulaeva, Roy Parker

Publication

Chekulaeva et al RNA. 2009 May;15(5):794-803. doi: 10.126

Insert

dGW182 (gw Fly)

Tags

HA

Expression

Insect

Promoter

Ac5

Available since

Dec. 11, 2017

Availability

Academic Institutions and Nonprofits only

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pMCh-139

Plasmid

#92287

Purpose

Plasmid for expression of NHA-tagged Drosophila melanogaster GW182 NED in D. melanogaster cells

Depositor

Marina Chekulaeva, Witold Filipowicz

Publication

Chekulaeva et al Nucleic Acids Res. 2010 Oct;38(19):6673-

Insert

dGW182 NED (gw Fly)

Tags

NHA

Expression

Insect

Mutation

dGW182 NED: aa 205-490

Promoter

Ac5

Available since

Oct. 2, 2017

Availability

Academic Institutions and Nonprofits only

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pMT-HA-STX4

Plasmid

#89311

Purpose

Expresses HA tagged syntaxin 4 in Drosophila cells

Depositor

Andrew Peden

Insert

STX4 (Syx4 Fly)

Tags

HA tag

Expression

Insect

Promoter

P-MT (Metallothionein)

Available since

Aug. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pSTAP-seq_fly-tj

Plasmid

#86386

Purpose

Vector to measure enhancer responsiveness of candidates from a genomic library (cloned instead of the core promoter) by determining the abundance of transcripts originating from each candidate.

Depositor

Alexander Stark

Publication

Arnold et al Nat Biotechnol. 2016 Dec 26. doi: 10.103

Insert

D. melanogaster tj enhancer

Use

Stap-seq screening vector

Available since

June 2, 2017

Availability

Academic Institutions and Nonprofits only

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pUAST-attB-yki:V5-S111A

Plasmid

#85619

Purpose

Expresses V5 tagged mutant yki in Drosophila.

Depositor

Kenneth Irvine

Publication

Oh et al Oncogene. 2009 Apr 30;28(17):1916-27. do

Insert

yki-V5-S111A

Tags

V5

Expression

Insect

Available since

May 5, 2017

Availability

Academic Institutions and Nonprofits only

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pUAST-attB-yki:V5-S250A

Plasmid

#85618

Purpose

Expresses V5 tagged mutant yki in Drosophila.

Depositor

Kenneth Irvine

Publication

Oh et al Oncogene. 2009 Apr 30;28(17):1916-27. do

Insert

yki-V5-S250A

Tags

V5

Expression

Insect

Available since

May 5, 2017

Availability

Academic Institutions and Nonprofits only

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pAc-HT-Dmp53KRKR

Plasmid

#85699

Purpose

expression of Drosophila p53 KRKR mutant in Drosophila cells

Depositor

Licio Collavin, Giannino Del Sal

Publication

Mauri et al J Biol Chem. 2008 Jul 25. 283(30):20848-

Insert

Dmp53 (p53 Fly)

Tags

His

Expression

Insect

Mutation

K26R K302R

Promoter

Ac5

Available since

March 21, 2017

Availability

Academic Institutions and Nonprofits only
pDHJS3 AN-

Plasmid

#78241

Purpose

One of four plasmids needed to create the mismatched double Holliday junction substrate (MM-DHJS).

Depositor

Tao-shih Hsieh

Publication

Plank et al J Biol Chem. 2006 Jun 23;281(25):17510-6

Inserts

loxP
sequence "A" with NheI site
loxP2

Available since

Sept. 26, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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57F03_GAL80

Plasmid

#83254

Purpose

Expresses GAL80 under the control of 57F03 enhancer

Depositor

Leslie Vosshall

Publication

Yapici et al Cell. 2016 Apr 21;165(3):715-29. doi: 10

Insert

57F03 enhancer

Expression

Insect

Available since

Sept. 26, 2016

Availability

Academic Institutions and Nonprofits only

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exon L-firefly

Plasmid

#81006

Purpose

luciferase based mini-gene to report splicing at exon 25 in Drosophila parayltic

Depositor

Richard Baines

Publication

Lin et al Brain. 2015 Apr;138(Pt 4):891-901. doi:

Insert

paralytic (para Fly)

Tags

firefly luciferase

Expression

Insect

Mutation

spanning exon 24 to exon 26, termination codon in…

Promoter

P-AC5 (Actin 5C)

Available since

Aug. 19, 2016

Availability

Academic Institutions and Nonprofits only

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UAS-Xbp1-GFP

Plasmid

#80627

Purpose

expression of Xbp1-GFP (RA isoform) in Drosophila

Depositor

Pedro Domingos, Herman Steller

Publication

Ryoo et al EMBO J. 2007 Jan 10;26(1):242-52. Epub 2

Insert

Xbp1 (Xbp1 Fly)

Tags

EGFP

Expression

Insect

Mutation

RA isoform

Promoter

UAS

Available since

July 28, 2016

Availability

Academic Institutions and Nonprofits only
pAc5.1B-EGFP-DmCCr4

Plasmid

#79252

Purpose

Expresses EGFP-tagged DmCCr4 in S2 cells

Depositor

Elisa Izaurralde

Publication

Haas et al J Cell Biol. 2010 Apr 19. 189(2):289-302

Insert

DmCaf40 (twin Fly)

Use

Schneider 2 cell expression

Tags

EGFP

Promoter

Ac5 promoter

Available since

July 19, 2016

Availability

Academic Institutions and Nonprofits only

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