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tsPurple chromoprotein

Plasmid

#117848

Purpose

BioBrick pSB1C3 plasmid that constitutively overexpresses tsPurple chromoprotein in E. coli

Depositor

Anthony Forster

Article

Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

Insert

promoter, RBS, tsPurple

Use

Synthetic Biology; Escherichia coli

Mutation

BioBrick sites removed

Available Since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pIVT-Flp-2A-BleoR

Plasmid

#232752

Purpose

IVT template for making bicistronic modRNA encoding Flippase and Zeocin resistance gene

Depositor

Kate Galloway

Article

Blanch-Asensio et al Nat Biomed Eng. 2026 Apr 30. doi: 10.103

Inserts

FlpO
BleoR

Use

In vitro transcription

Available Since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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Bxb1-GT_CAG-ITPKA-mScarlet-I

Plasmid

#229778

Purpose

Bxb1-GT donor plasmid for integrating a transcription unit for constitutive expression of a mScarlet-I F-actin reporter

Depositor

Richard Davis

Article

Blanch-Asensio et al Nat Biomed Eng. 2026 Apr 30. doi: 10.103

Insert

mScarlet-I

Use

Synthetic Biology

Tags

ITPKA

Expression

Mammalian

Promoter

CAG

Available Since

March 6, 2025

Availability

Academic Institutions and Nonprofits only

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gfasPurple chromoprotein

Plasmid

#117849

Purpose

BioBrick pSB1C3 plasmid that constitutively overexpresses gfasPurple chromoprotein in E. coli

Depositor

Anthony Forster

Article

Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

Insert

promoter, RBS, gfasPurple

Use

Synthetic Biology; Escherichia coli

Mutation

BioBrick sites removed

Available Since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

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CLYBL_Bxb1-GA_LP_Dual_TC

Plasmid

#229776

Purpose

CLYBL targeting construct with the Bxb1-GA landing pad cassette.

Depositor

Richard Davis

Article

Blanch-Asensio et al Nat Biomed Eng. 2026 Apr 30. doi: 10.103

Insert

FRT-mScarlet-attP(GA)-PuroR-F3

Use

Synthetic Biology

Expression

Mammalian

Promoter

pGK

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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AAV-eGFP-KASH

Plasmid

#248781

Purpose

AAV construct expressing eGFP-KASH enabling FACS-sorting of extracted nuclei

Depositor

David Liu

Article

Levy et al Nat Biomed Eng. 2020 Jan;4(1):97-110. do

Insert

eGFP-KASH

Promoter

Cbh

Available Since

Jan. 5, 2026

Availability

Academic Institutions and Nonprofits only

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aeBlue chromoprotein

Plasmid

#117846

Purpose

BioBrick pSB1C3 plasmid that constitutively overexpresses aeBlue chromoprotein in E. coli

Depositor

Anthony Forster

Article

Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

Insert

promoter, RBS, aeBlue

Use

Synthetic Biology; Escherichia coli

Mutation

BioBrick sites removed

Available Since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

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CMV-SauriABE8e

Plasmid

#189927

Purpose

Plasmid encoding SauriABE8e driven by the CMV promoter for plasmid transfection.

Depositor

David Liu

Article

Davis et al Nat Biomed Eng. 2022 Jul 28. pii: 10.103

Insert

SauriABE8e

Expression

Mammalian

Mutation

Nme2Cas9 D16A

Promoter

CMV

Available Since

Sept. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pBS3Clux

Plasmid

#55172

Purpose

lux-reporter vector, integration at sacA, ampr, cmr

Depositor

Thorsten Mascher

Article

Radeck et al J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.

Type

Empty backbone

Use

Synthetic Biology; Bacillus biobrick box

Promoter

none

Available Since

July 22, 2014

Availability

Academic Institutions and Nonprofits only

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eforRed chromoprotein

Plasmid

#117837

Purpose

BioBrick pSB1C3 plasmid that constitutively overexpresses eforRed chromoprotein in E. coli

Depositor

Anthony Forster

Article

Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

Insert

promoter, RBS, eforRed

Use

Synthetic Biology; Escherichia coli

Mutation

BioBrick sites removed

Available Since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pCHC005 (∆RE)

Plasmid

#226199

Purpose

Conjugation knockout plasmid (pK18mobsacB) to delete H16_0006 (Type I restriction endonuclease subunit).

Depositor

Christopher Johnson

Article

Calvey et al Metab Eng. 2023 Jan;75:78-90. doi: 10.10

Insert

Homology arms for type I restriction-modification system restriction subunit H16_A0006

Use

Synthetic Biology

Available Since

Nov. 18, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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CMV-Nme2ABE8e

Plasmid

#189928

Purpose

Plasmid encoding Nme2ABE8e driven by the CMV promoter for plasmid transfection.

Depositor

David Liu

Article

Davis et al Nat Biomed Eng. 2022 Jul 28. pii: 10.103

Insert

Nme2ABE8e

Tags

NLS

Expression

Mammalian

Mutation

Nme2Cas9 D16A

Promoter

CMV

Available Since

Sept. 7, 2022

Availability

Academic Institutions and Nonprofits only

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amajLime chromoprotein

Plasmid

#117843

Purpose

BioBrick pSB1C3 plasmid that constitutively overexpresses amajLime chromoprotein in E. coli

Depositor

Anthony Forster

Article

Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

Insert

promoter, RBS, amajLime

Use

Synthetic Biology; Escherichia coli

Mutation

BioBrick sites removed

Available Since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

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CMV-CjABE8e

Plasmid

#189929

Purpose

Plasmid encoding CjABE8e driven by the CMV promoter for plasmid transfection.

Depositor

David Liu

Article

Davis et al Nat Biomed Eng. 2022 Jul 28. pii: 10.103

Insert

CjABE8e

Expression

Mammalian

Mutation

CjCas9 D8A

Promoter

CMV

Available Since

Sept. 7, 2022

Availability

Academic Institutions and Nonprofits only

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fwYellow chromoprotein

Plasmid

#117841

Purpose

BioBrick pSB1C3 plasmid that constitutively overexpresses fwYellow chromoprotein in E. coli

Depositor

Anthony Forster

Article

Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

Insert

promoter, RBS, fwYellow

Use

Synthetic Biology; Escherichia coli

Mutation

BioBrick sites removed

Available Since

Aug. 7, 2019

Availability

Academic Institutions and Nonprofits only

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scOrange chromoprotein

Plasmid

#117840

Purpose

BioBrick pSB1C3 plasmid that constitutively overexpresses scOrange chromoprotein in E. coli

Depositor

Anthony Forster

Article

Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

Insert

promoter, RBS, scOrange

Use

Synthetic Biology; Escherichia coli

Mutation

BioBrick sites removed

Available Since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pCHC027 (∆pemK)

Plasmid

#226204

Purpose

Conjugation knockout plasmid (pK18msB) to delete the C. necator addiction system toxin PemK, to enable deletion of the pHG1 megaplasmid.

Depositor

Christopher Johnson

Article

Calvey et al Metab Eng. 2023 Jan;75:78-90. doi: 10.10

Insert

Upstream/downstream homology arms for addiction system toxinPemK.

Use

Synthetic Biology

Available Since

Nov. 14, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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spisPink chromoprotein

Plasmid

#117839

Purpose

BioBrick pSB1C3 plasmid that constitutively overexpresses spisPink chromoprotein in E. coli

Depositor

Anthony Forster

Article

Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

Insert

promoter, RBS, spisPink

Use

Synthetic Biology; Escherichia coli

Mutation

BioBrick sites removed

Available Since

July 31, 2019

Availability

Academic Institutions and Nonprofits only

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amilCP_Pink chromoprotein

Plasmid

#117851

Purpose

BioBrick pSB1C3 plasmid that constitutively overexpresses amilCP_Pink chromoprotein in E. coli

Depositor

Anthony Forster

Article

Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

Insert

promoter, RBS, amilCP_Pink

Use

Synthetic Biology; Escherichia coli

Mutation

64A,65C, BioBrick sites removed

Available Since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

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Bxb1-GT_hEF1a-mGL_CAG-mRuby2_Divergent

Plasmid

#229800

Purpose

Bxb1-GT donor plasmid with divergent syntax for constitutively expressing mRuby2 and mGreenLantern by the respective promoters CAG and hEF1a

Depositor

Richard Davis

Article

Blanch-Asensio et al Nat Biomed Eng. 2026 Apr 30. doi: 10.103

Insert

mGreenLantern; mRuby2

Use

Synthetic Biology

Expression

Mammalian

Promoter

hEF1a and CAG

Available Since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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asPink chromoprotein

Plasmid

#117838

Purpose

BioBrick pSB1C3 plasmid that constitutively overexpresses asPink chromoprotein in E. coli

Depositor

Anthony Forster

Article

Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

Insert

promoter, RBS, asPink

Use

Synthetic Biology; Escherichia coli

Mutation

BioBrick sites removed

Available Since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

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amilCP_Orange chromoprotein

Plasmid

#117850

Purpose

BioBrick pSB1C3 plasmid that constitutively overexpresses amilCP_Orange chromoprotein in E. coli

Depositor

Anthony Forster

Article

Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

Insert

promoter, RBS, amilCP_Orange

Use

Synthetic Biology; Escherichia coli

Mutation

64V,65G, BioBrick sites removed

Available Since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

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amilGFP chromoprotein

Plasmid

#117842

Purpose

BioBrick pSB1C3 plasmid that constitutively overexpresses amilGFP chromoprotein in E. coli

Depositor

Anthony Forster

Article

Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

Insert

promoter, RBS, amilGFP

Use

Synthetic Biology; Escherichia coli

Mutation

BioBrick sites removed

Available Since

July 31, 2019

Availability

Academic Institutions and Nonprofits only

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pBS1ClacZ

Plasmid

#55171

Purpose

lacZ-reporter vector, integration at amyE, ampr, cmr

Depositor

Thorsten Mascher

Article

Radeck et al J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.

Type

Empty backbone

Use

Synthetic Biology; Bacillus biobrick box

Promoter

none

Available Since

Aug. 6, 2014

Availability

Academic Institutions and Nonprofits only
Bxb1-GT_AIO-TetOn_Divergent Donor

Plasmid

#229784

Purpose

Bxb1-GT donor plasmid with divergent syntax for all-in-one doxycycline-inducible expression (empty)

Depositor

Richard Davis

Article

Blanch-Asensio et al Nat Biomed Eng. 2026 Apr 30. doi: 10.103

Insert

LacZ; rtTA-T2A-mTagBFP2

Use

Synthetic Biology

Expression

Mammalian

Promoter

TRE and CAG

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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meffBlue chromoprotein

Plasmid

#117845

Purpose

BioBrick pSB1C3 plasmid that constitutively overexpresses meffBlue chromoprotein in E. coli

Depositor

Anthony Forster

Article

Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

Insert

promoter, RBS, meffBlue

Use

Synthetic Biology; Escherichia coli

Mutation

BioBrick sites removed

Available Since

Aug. 7, 2019

Availability

Academic Institutions and Nonprofits only

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Bxb1-GA_AIO-TetOn_mScarlet_Divergent

Plasmid

#229791

Purpose

Bxb1-GA donor plasmid with divergent syntax for all-in-one doxycycline-inducible mScarlet expression

Depositor

Richard Davis

Article

Blanch-Asensio et al Nat Biomed Eng. 2026 Apr 30. doi: 10.103

Insert

TRE-mScarlet; rtTA-T2A-mTagBFP2

Use

Synthetic Biology

Tags

NLS

Expression

Mammalian

Promoter

TRE and CAG

Available Since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti-NG-PE2-BSD

Plasmid

#176933

Purpose

Expresses PE2-NG variant in mammalian cells

Depositor

Hyongbum Kim

Article

Jang et al Nat Biomed Eng. 2021 Aug 26. pii: 10.103

Insert

PE2-NG variant

Use

Lentiviral

Tags

SV40 NLS

Expression

Mammalian

Promoter

EFS

Available Since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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cjBlue chromoprotein

Plasmid

#117844

Purpose

BioBrick pSB1C3 plasmid that constitutively overexpresses cjBlue chromoprotein in E. coli

Depositor

Anthony Forster

Article

Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

Insert

promoter, RBS, cjBlue

Use

Synthetic Biology; Escherichia coli

Mutation

BioBrick sites removed

Available Since

July 31, 2019

Availability

Academic Institutions and Nonprofits only

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pCA24N-ligase-NLS-cTF

Plasmid

#87746

Purpose

IPTG-inducible expression of ligase-NLS-cTF fusion for protein purification

Depositor

Wayne Patrick

Article

Wilson et al Protein Eng Des Sel. 2013 Jul;26(7):471-

Insert

T4 DNA ligase (30 )

Tags

6xHis and NLS linker - cTF (chimeric transcriptio…

Expression

Bacterial

Promoter

T5-lac

Available Since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

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