We narrowed to 4,504 results for: pcr

1. pHyg-CUbo-9myc

   Plasmid

   #212786

   Purpose

   C-terminal PCR-based tagging with CUbo-9myc in budding yeast with HygR selection

   Depositor

   Helle Ulrich

   Article

   Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

   Type

   Empty backbone

   Use

   Pcr-based tagging

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pHyg-CUbo(K63R)-9myc

   Plasmid

   #212788

   Purpose

   C-terminal PCR-based tagging with CUbo(K63R)-9myc in budding yeast with HygR selection

   Depositor

   Helle Ulrich

   Article

   Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

   Type

   Empty backbone

   Use

   Pcr-based tagging

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pHyg-CUbo(K48R/K63R)-9myc

   Plasmid

   #212789

   Purpose

   C-terminal PCR-based tagging with CUbo(K48R/K63R)-9myc in budding yeast with HygR selection

   Depositor

   Helle Ulrich

   Article

   Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

   Type

   Empty backbone

   Use

   Pcr-based tagging

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pHyg-CUbo-AID*-GFP

   Plasmid

   #212770

   Purpose

   C-terminal PCR-based tagging with CUbo-AID*-yeGFP in budding yeast with HygR selection

   Depositor

   Helle Ulrich

   Article

   Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

   Type

   Empty backbone

   Use

   Pcr-based tagging

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pUra3-pCUP1-CUbo(K48R/K63R)

   Plasmid

   #212765

   Purpose

   N-terminal PCR-based tagging with pCUP1-CUbo(K48R/K63R) in budding yeast with URA3 selection

   Depositor

   Helle Ulrich

   Article

   Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

   Type

   Empty backbone

   Use

   Pcr-based tagging

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pUra3-pCUP1-CUbo(K63R)

   Plasmid

   #212764

   Purpose

   N-terminal PCR-based tagging with pCUP1-CUbo(K63R) in budding yeast with URA3 selection

   Depositor

   Helle Ulrich

   Article

   Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

   Type

   Empty backbone

   Use

   Pcr-based tagging

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pUra3-pCUP1-CUbo(K48R)

   Plasmid

   #212763

   Purpose

   N-terminal PCR-based tagging with pCUP1-CUbo(K48R) in budding yeast with URA3 selection

   Depositor

   Helle Ulrich

   Article

   Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

   Type

   Empty backbone

   Use

   Pcr-based tagging

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pUra3-pCUP1-CUbo

   Plasmid

   #212762

   Purpose

   N-terminal PCR-based tagging with pCUP1-CUbo in budding yeast with URA3 selection

   Depositor

   Helle Ulrich

   Article

   Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

   Type

   Empty backbone

   Use

   Pcr-based tagging

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pUra3-pCUP1-His7-NUbo

   Plasmid

   #212761

   Purpose

   N-terminal PCR-based tagging with pCUP1-His7-NUbo in budding yeast with URA3 selection

   Depositor

   Helle Ulrich

   Article

   Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

   Type

   Empty backbone

   Use

   Pcr-based tagging

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pUra3-pCUP1-NUbo

    Plasmid

    #212760

    Purpose

    N-terminal PCR-based tagging with pCUP1-NUbo in budding yeast with URA3 selection

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Type

    Empty backbone

    Use

    Pcr-based tagging

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pFA6a-His3MX6-PGAL1-GST

    Plasmid

    #41613

    Depositor

    John Pringle

    Article

    Longtine et al Yeast. 1998 Jul;14(10):953-61.

    Insert

    GAL1 promoter (GAL1 Budding Yeast)
    

    Use

    Yeast genomic targeting

    Tags

    A. gossypii translation elongation factor 1a gene…

    Expression

    Mutation

    Promoter

    Available since

    Dec. 11, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pFA6a-TRP1-PGAL1-HBTH

    Plasmid

    #26898

    Depositor

    Peter Kaiser

    Insert

    HBTH tag N-terminal
    

    Use

    Pcr tagging yeast

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 5, 2011

    Availability

    Academic Institutions and Nonprofits only

13. pFA6a-kanMX6-P3nmt1-GFP

    Plasmid

    #39289

    Depositor

    Jurg Bahler, John Pringle

    Article

    Bahler et al Yeast. 1998 Jul;14(10):943-51.

    Insert

    nmt1 promoter (nmt1 Fission Yeast)
    

    Use

    Yeast genomic targeting

    Tags

    A. gossypii translation elongation factor 1a gene…

    Expression

    Mutation

    -1163 to +6 of nmt1 locus

    Promoter

    Available since

    Sept. 25, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pFA6a-kanMX6-P41nmt1

    Plasmid

    #39281

    Depositor

    Jurg Bahler, John Pringle

    Article

    Bahler et al Yeast. 1998 Jul;14(10):943-51.

    Insert

    nmt1 promoter (nmt1 Fission Yeast)
    

    Use

    Yeast genomic targeting

    Tags

    A. gossypii translation elongation factor 1a gene…

    Expression

    Mutation

    -1163 to +6 of nmt1 locus with a 4bp deletion (TA…

    Promoter

    Available since

    Sept. 25, 2012

    Availability

    Academic Institutions and Nonprofits only

15. pFA6a-kanMX6-P81nmt1

    Plasmid

    #39282

    Depositor

    Jurg Bahler, John Pringle

    Article

    Bahler et al Yeast. 1998 Jul;14(10):943-51.

    Insert

    nmt1 promoter (nmt1 Fission Yeast)
    

    Use

    Yeast genomic targeting

    Tags

    A. gossypii translation elongation factor 1a gene…

    Expression

    Mutation

    -1163 to +6 of nmt1 locus with a 7bp deletion (AT…

    Promoter

    Available since

    Sept. 25, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pFA6a-kanMX6-P3nmt1-3HA

    Plasmid

    #39283

    Depositor

    Jurg Bahler, John Pringle

    Article

    Bahler et al Yeast. 1998 Jul;14(10):943-51.

    Insert

    nmt1 promoter (nmt1 Fission Yeast)
    

    Use

    Yeast genomic targeting

    Tags

    3xHA, A. gossypii translation elongation factor 1…

    Expression

    Mutation

    -1163 to +6 of nmt1 locus

    Promoter

    Available since

    Aug. 30, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pGC(2.1)-U6.3

    Plasmid

    #170516

    Purpose

    PCR template vector for amplifying gRNAcore(2.1)-pU6.3 promoter fragment; used together with pAC-CR7T-gRNA2.1-nlsBFP

    Depositor

    Chun Han

    Article

    Koreman et al Proc Natl Acad Sci U S A. 2021 Apr 6;118

    Insert

    gRNAcore(2.1)-pU6.3
    

    Use

    Pcr template vector for amplifying grnacore(2.1)-…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pFA6a-kanMX6-P3nmt1-GST

    Plasmid

    #39286

    Depositor

    Jurg Bahler, John Pringle

    Article

    Bahler et al Yeast. 1998 Jul;14(10):943-51.

    Insert

    nmt1 promoter (nmt1 Fission Yeast)
    

    Use

    Yeast genomic targeting

    Tags

    A. gossypii translation elongation factor 1a gene…

    Expression

    Mutation

    -1163 to +6 of nmt1 locus

    Promoter

    Available since

    Sept. 21, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pHyg-CUbo(K48R/K63R)-yeGFP-His6

    Plasmid

    #212785

    Purpose

    C-terminal PCR-based tagging with CUbo(K48R/K63R)-yeGFP-His6 in budding yeast with HygR selection

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Type

    Empty backbone

    Use

    Pcr-based tagging

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pRS416-Syf1

    Plasmid

    #234113

    Purpose

    Syf1 region PCR, restriction digest and ligation into pRS416 backbone

    Depositor

    Aaron Hoskins

    Insert

    Syf1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pRS414-Syf1

    Plasmid

    #234114

    Purpose

    Syf1 region PCR, restriction digest and ligation into pRS414 backbone

    Depositor

    Aaron Hoskins

    Insert

    Syf1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pFA6a-kanMX6-P41nmt-3HA

    Plasmid

    #39284

    Depositor

    Jurg Bahler, John Pringle

    Article

    Bahler et al Yeast. 1998 Jul;14(10):943-51.

    Insert

    nmt1 promoter (nmt1 Fission Yeast)
    

    Use

    Yeast genomic targeting

    Tags

    3xHA, A. gossypii translation elongation factor 1…

    Expression

    Mutation

    -1163 to +6 of nmt1 locus with a 4bp deletion (TA…

    Promoter

    Available since

    Aug. 30, 2012

    Availability

    Academic Institutions and Nonprofits only

23. pFA6a-kanMX6-P81nmt1-3HA

    Plasmid

    #39285

    Depositor

    Jurg Bahler, John Pringle

    Article

    Bahler et al Yeast. 1998 Jul;14(10):943-51.

    Insert

    nmt1 promoter (nmt1 Fission Yeast)
    

    Use

    Yeast genomic targeting

    Tags

    3xHA, A. gossypii translation elongation factor 1…

    Expression

    Mutation

    -1163 to +6 of nmt1 locus with a 7bp deletion (AT…

    Promoter

    Available since

    Aug. 14, 2012

    Availability

    Academic Institutions and Nonprofits only

24. pFA6a-kanMX6-P41nmt1-GFP

    Plasmid

    #39290

    Depositor

    Jurg Bahler, John Pringle

    Article

    Bahler et al Yeast. 1998 Jul;14(10):943-51.

    Insert

    nmt1 promoter (nmt1 Fission Yeast)
    

    Use

    Yeast genomic targeting

    Tags

    A. gossypii translation elongation factor 1a gene…

    Expression

    Mutation

    -1163 to +6 of nmt1 locus with a 4bp deletion (TA…

    Promoter

    Available since

    Aug. 20, 2012

    Availability

    Academic Institutions and Nonprofits only

25. pFA6a-kanMX6-P81nmt1-GFP

    Plasmid

    #39291

    Depositor

    Jurg Bahler, John Pringle

    Article

    Bahler et al Yeast. 1998 Jul;14(10):943-51.

    Insert

    nmt1 promoter (nmt1 Fission Yeast)
    

    Use

    Yeast genomic targeting

    Tags

    A. gossypii translation elongation factor 1a gene…

    Expression

    Mutation

    -1163 to +6 of nmt1 locus with a 7bp deletion (AT…

    Promoter

    Available since

    Aug. 20, 2012

    Availability

    Academic Institutions and Nonprofits only

26. pFA6a-kanMX6-P41nmt1-GST

    Plasmid

    #39287

    Depositor

    Jurg Bahler, John Pringle

    Article

    Bahler et al Yeast. 1998 Jul;14(10):943-51.

    Insert

    nmt1 promoter (nmt1 Fission Yeast)
    

    Use

    Yeast genomic targeting

    Tags

    A. gossypii translation elongation factor 1a gene…

    Expression

    Mutation

    -1163 to +6 of nmt1 locus with a 4bp deletion (TA…

    Promoter

    Available since

    Sept. 17, 2012

    Availability

    Academic Institutions and Nonprofits only

27. P63-sp64 Poly A

    Plasmid

    #59435

    Purpose

    synthetic poly A transcripts of VZV

    Depositor

    Randall Cohrs

    Article

    Cohrs et al J Virol. 2000 Dec;74(24):11464-71.

    Insert

    VZV ORF 63
    

    Use

    Makes synthetic transcripts that contains poly a

    Tags

    none

    Expression

    Mutation

    none

    Promoter

    SP6

    Available since

    Sept. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

28. P29-sp64 Poly A

    Plasmid

    #59434

    Purpose

    synthetic poly A transcripts of VZV

    Depositor

    Randall Cohrs

    Article

    Cohrs et al J Virol. 2000 Dec;74(24):11464-71.

    Insert

    VZV ORF 29
    

    Use

    Makes synthetic transcripts that contains poly a

    Tags

    none

    Expression

    Mutation

    none

    Promoter

    SP6

    Available since

    Sept. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

29. P21-sp64 Poly A

    Plasmid

    #59433

    Purpose

    synthetic poly A transcripts of VZV

    Depositor

    Randall Cohrs

    Article

    Cohrs et al J Virol. 2000 Dec;74(24):11464-71.

    Insert

    VZV ORF 21
    

    Use

    Makes synthetic transcripts that contains poly a

    Tags

    none

    Expression

    Mutation

    none

    Promoter

    SP6

    Available since

    Sept. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

30. pMVQA

    Plasmid

    #197894

    Purpose

    pCR®2.1-TOPO® backbone with 3' end of HIV-1(HXB2:9045 till poly-A (20))

    Depositor

    Thomas Klimkait

    Article

    Otte et al Cell Rep Methods. 2023 May 23;3(6):10048

    Insert

    HIV-1 3' end (HXB2: 9045 till poly-A(10)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge