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Showing: 1701 - 1750 of 79968 results

  1. pUC-L1L2

    Plasmid
    #114017
    Purpose
    Create Entry clones for Gateway LR reactions through simple restriction enzyme based cloning
    Depositor
    Christopher Grefen
    Insert
    ccdB gene, Chloramphenicol resistance gene
    Use
    Gateway Cloning
    Available Since
    Jan. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    114017-plasmid-map-sequence-id-222768

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  2. 371-A

    Plasmid
    #97026
    Purpose
    yORF
    Depositor
    Chris Jeans
    Type
    Empty backbone
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    July 11, 2017
    Availability
    Academic Institutions and Nonprofits only
    97026-plasmid-map-sequence-id-182505

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  3. Flag-APEX3-C Cloning Vector

    Plasmid
    #220615
    Purpose
    To express a protein of interest fused to the C-terminus of Flag-APEX3 for proximity labeling or TEM (unpublished)
    Depositor
    Ken-Ichi Takemaru
    Type
    Empty backbone
    Tags
    Flag-APEX3 fusion
    Expression
    Mammalian
    Available Since
    June 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    220615-plasmid-map-sequence-id-435941

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  4. pOpen-SfiIR (cDNA)

    Plasmid
    #165528
    Purpose
    Type II restriction enzyme that recognizes the double-stranded sequence GGCCNNNNNGGCC and cleaves after N-4.
    Depositor
    Drew Endy, Jennifer Molloy, FreeGenes Project
    Insert
    SfiI (used to create cDNA libraries)
    Use
    Synthetic Biology
    Available Since
    Aug. 5, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165528-plasmid-map-sequence-id-339067

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  6. pSLfa[Tc'hsp5'3'UTR]fa

    Plasmid
    #86450
    Purpose
    Heat shock-inducible promoter including hsp-UTRs, ubiquitous expression upon heat-shock of any inserted gene of interest in Tribolium; (Shuttle plasmid)
    Depositor
    Gregor Bucher
    Insert
    Tc'hsp5'3'UTR
    Expression
    Insect
    Available Since
    March 22, 2017
    Availability
    Academic Institutions and Nonprofits only
    86450-plasmid-map-sequence-id-171108

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  7. Flag-2xAPEX2-C Cloning Vector

    Plasmid
    #136991
    Purpose
    To express a protein of interest fused to the C-terminus of Flag-2xAPEX2 for proximity labeling or TEM
    Depositor
    Ken-Ichi Takemaru
    Type
    Empty backbone
    Tags
    Flag
    Expression
    Mammalian
    Available Since
    Feb. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    136991-plasmid-map-sequence-id-271567

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  8. 371-U

    Plasmid
    #97036
    Purpose
    MYFQSNA-yORF
    Depositor
    Chris Jeans
    Type
    Empty backbone
    Tags
    MYFQSNA
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    July 24, 2017
    Availability
    Academic Institutions and Nonprofits only
    97036-plasmid-map-sequence-id-182502

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  9. pLenti-sgRNA

    Plasmid
    #71409
    Purpose
    There is no gene/insert. It is a backbone plasmid that others can use to insert sgRNAs of interest. The cloning site for this BsmBI
    Depositor
    Eric Lander, David Sabatini
    Type
    Empty backbone
    Use
    Lentiviral
    Available Since
    Jan. 14, 2016
    Availability
    Academic Institutions and Nonprofits only
    71409-plasmid-map-sequence-id-322475

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  10. pSNAP-tag (T7) Vector

    Plasmid
    #101137
    Purpose
    Expression of protein of interest with SNAP-Tag in bacterial cells
    Depositor
    New England Biolabs, Ana Egana
    Type
    Empty backbone
    Tags
    SNAP-tag (SNAP26b)
    Expression
    Bacterial
    Available Since
    Sept. 27, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    101137-plasmid-map-sequence-id-194162

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  11. pDEST-Spe4-RfA-mScarlet-I-stop

    Plasmid
    #186393
    Purpose
    Destination vector for the expression of a fluorescent fusion of a protein of interest with C-ter mScarlet-I into embryos, by mRNA microinjection
    Depositor
    Patrick Lemaire
    Type
    Empty backbone
    Use
    Gateway Cloning
    Available Since
    Sept. 29, 2022
    Availability
    Academic Institutions and Nonprofits only
    186393-plasmid-map-sequence-id-373587

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  12. pDEST-Spe4-Kozak-miRFP713-RfA

    Plasmid
    #186381
    Purpose
    Destination vector for the expression of a fluorescent fusion of N-ter miRFP713 and a protein of interest into embryos, by mRNA microinjection
    Depositor
    Patrick Lemaire
    Type
    Empty backbone
    Use
    Gateway Cloning
    Available Since
    May 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    186381-plasmid-map-sequence-id-392698

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  13. pDEST-Spe4-RfA-miRFP713-stop

    Plasmid
    #186391
    Purpose
    Destination vector for the expression of a fluorescent fusion of a protein of interest with C-ter miRFP713 into embryos, by mRNA microinjection
    Depositor
    Patrick Lemaire
    Type
    Empty backbone
    Use
    Gateway Cloning
    Available Since
    Oct. 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    186391-plasmid-map-sequence-id-373838

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  14. pDEST-Spe4-RfA-emiRFP703-stop

    Plasmid
    #186386
    Purpose
    Destination vector for the expression of a fluorescent fusion of a protein of interest with C-ter emiRFP703 into embryos, by mRNA microinjection
    Depositor
    Patrick Lemaire
    Type
    Empty backbone
    Use
    Gateway Cloning
    Available Since
    Oct. 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    186386-plasmid-map-sequence-id-373831

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  15. pDEST-Spe4-Kozak-emiRFP703-RfA

    Plasmid
    #186376
    Purpose
    Destination vector for the expression of a fluorescent fusion of N-ter emiRFP703 and a protein of interest into embryos, by mRNA microinjection
    Depositor
    Patrick Lemaire
    Type
    Empty backbone
    Use
    Gateway Cloning
    Available Since
    June 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    186376-plasmid-map-sequence-id-398283

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  16. pDEST-Spe4-RfA-mVenus-stop

    Plasmid
    #186394
    Purpose
    Destination vector for the expression of a fluorescent fusion of a protein of interest with C-ter mVenus into embryos, by mRNA microinjection
    Depositor
    Patrick Lemaire
    Type
    Empty backbone
    Use
    Gateway Cloning
    Available Since
    May 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    186394-plasmid-map-sequence-id-392701

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  17. pDEST-Spe4-RfA-mNeonGreen-stop

    Plasmid
    #186392
    Purpose
    Destination vector for the expression of a fluorescent fusion of a protein of interest with C-ter mNeonGreen into embryos, by mRNA microinjection
    Depositor
    Patrick Lemaire
    Type
    Empty backbone
    Use
    Gateway Cloning
    Available Since
    Oct. 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    186392-plasmid-map-sequence-id-373841

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  18. pDEST-Spe4-RfA-mEos4b-stop

    Plasmid
    #186390
    Purpose
    Destination vector for the expression of a fluorescent fusion of a protein of interest with C-ter mEos4b into embryos, by mRNA microinjection
    Depositor
    Patrick Lemaire
    Type
    Empty backbone
    Use
    Gateway Cloning
    Available Since
    Oct. 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    186390-plasmid-map-sequence-id-373835

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  19. pDEST-Spe4-RfA-mClover3-stop

    Plasmid
    #186389
    Purpose
    Destination vector for the expression of a fluorescent fusion of a protein of interest with C-ter mClover3 into embryos, by mRNA microinjection
    Depositor
    Patrick Lemaire
    Type
    Empty backbone
    Use
    Gateway Cloning
    Available Since
    Oct. 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    186389-plasmid-map-sequence-id-373834

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  20. pDEST-Spe4-Rfa-mCherry-stop

    Plasmid
    #186388
    Purpose
    Destination vector for the expression of a fluorescent fusion of a protein of interest with C-ter mCherry into embryos, by mRNA microinjection
    Depositor
    Patrick Lemaire
    Type
    Empty backbone
    Use
    Gateway Cloning
    Available Since
    Oct. 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    186388-plasmid-map-sequence-id-373832

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  21. pDEST-Spe4-Kozak-mVenus-RfA

    Plasmid
    #186384
    Purpose
    Destination vector for the expression of a fluorescent fusion of N-ter mVenus and a protein of interest into embryos, by mRNA microinjection
    Depositor
    Patrick Lemaire
    Type
    Empty backbone
    Use
    Gateway Cloning
    Available Since
    Oct. 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    186384-plasmid-map-sequence-id-373827

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  22. pDEST-Spe4-Kozak-mScarlet-I-RfA

    Plasmid
    #186383
    Purpose
    Destination vector for the expression of a fluorescent fusion of N-ter mScarlet-I and a protein of interest into embryos, by mRNA microinjection
    Depositor
    Patrick Lemaire
    Type
    Empty backbone
    Use
    Gateway Cloning
    Available Since
    Oct. 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    186383-plasmid-map-sequence-id-373826

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  23. pDEST-Spe4-Kozak-mNeonGreen-RfA

    Plasmid
    #186382
    Purpose
    Destination vector for the expression of a fluorescent fusion of N-ter mNeonGreen and a protein of interest into embryos, by mRNA microinjection
    Depositor
    Patrick Lemaire
    Type
    Empty backbone
    Use
    Gateway Cloning
    Available Since
    Oct. 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    186382-plasmid-map-sequence-id-373805

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  24. pDEST-Spe4-Kozak-mEos4b-RfA

    Plasmid
    #186380
    Purpose
    Destination vector for the expression of a fluorescent fusion of N-ter mEos4b and a protein of interest into embryos, by mRNA microinjection
    Depositor
    Patrick Lemaire
    Type
    Empty backbone
    Use
    Gateway Cloning
    Available Since
    Oct. 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    186380-plasmid-map-sequence-id-373797

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  25. pDEST-Spe4-Kozak-mClover3-RfA

    Plasmid
    #186379
    Purpose
    Destination vector for the expression of a fluorescent fusion of N-ter mClover3 and a protein of interest into embryos, by mRNA microinjection
    Depositor
    Patrick Lemaire
    Type
    Empty backbone
    Use
    Gateway Cloning
    Available Since
    Sept. 30, 2022
    Availability
    Academic Institutions and Nonprofits only
    186379-plasmid-map-sequence-id-373710

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  26. pDEST-Spe4-Kozak-mCherry-RfA

    Plasmid
    #186378
    Purpose
    Destination vector for the expression of a fluorescent fusion of N-ter mCherry and a protein of interest into embryos, by mRNA microinjection
    Depositor
    Patrick Lemaire
    Type
    Empty backbone
    Use
    Gateway Cloning
    Available Since
    Sept. 30, 2022
    Availability
    Academic Institutions and Nonprofits only
    186378-plasmid-map-sequence-id-373704

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  27. pLKO.3G_X7

    Plasmid
    #171213
    Purpose
    3rd gen lentiviral backbone for cloning and expression of new shRNA sequences. Uses eGFP for selection. Please see cloning information for suggested restriction sites
    Depositor
    Jenny Wang
    Insert
    N/A; empty backbone
    Use
    Lentiviral and RNAi
    Expression
    Mammalian
    Available Since
    July 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    171213-plasmid-map-sequence-id-338282

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  28. pCDH-CB-IRES-copGFP-T2A-Puro

    Plasmid
    #72299
    Purpose
    Express gene of interest under CB promoter
    Depositor
    Kazuhiro Oka
    Type
    Empty backbone
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    chicken beta-actin + CMV enhancer
    Available Since
    Jan. 25, 2016
    Availability
    Industry, Academic Institutions, and Nonprofits
    72299-plasmid-map-sequence-id-131901

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  29. pBIR-GFP

    Plasmid
    #49807
    Purpose
    In vivo GFP based system to measure break induced replication (BIR) repair efficiency. expression of I-SceI generates a double strand break that when repaired by BIR mechanism will restore GFP.
    Depositor
    Thanos Halazonetis
    Insert
    GFP
    Expression
    Mammalian
    Promoter
    beta-actin
    Available Since
    Jan. 10, 2014
    Availability
    Academic Institutions and Nonprofits only
    49807-plasmid-map-sequence-id-74303

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  30. pDEST-Spe4-RfA-Halotag-stop

    Plasmid
    #186387
    Purpose
    Destination vector for the expression of a fluorescent fusion of a protein of interest with C-ter Halo tag into embryos, by mRNA microinjection
    Depositor
    Patrick Lemaire
    Type
    Empty backbone
    Use
    Gateway Cloning
    Available Since
    Sept. 29, 2022
    Availability
    Academic Institutions and Nonprofits only
    186387-plasmid-map-sequence-id-373586

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  31. pDEST-Spe4-Kozak-Snaptag-RfA

    Plasmid
    #186385
    Purpose
    Destination vector for the expression of a fluorescent fusion of N-ter Snap tag and a protein of interest into embryos, by mRNA microinjection
    Depositor
    Patrick Lemaire
    Type
    Empty backbone
    Use
    Gateway Cloning
    Available Since
    Oct. 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    186385-plasmid-map-sequence-id-373828

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  32. 371-Bc

    Plasmid
    #97028
    Purpose
    yORF-tev-His6
    Depositor
    Chris Jeans
    Type
    Empty backbone
    Tags
    His6 tag and TEV cleavage site
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    July 24, 2017
    Availability
    Academic Institutions and Nonprofits only
    97028-plasmid-map-sequence-id-182499

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  33. 371-B

    Plasmid
    #97027
    Purpose
    His6-tev-yORF
    Depositor
    Chris Jeans
    Type
    Empty backbone
    Tags
    His6 and TEV cleavage site
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    July 11, 2017
    Availability
    Academic Institutions and Nonprofits only
    97027-plasmid-map-sequence-id-182504

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  34. pDEST-Spe4-Kozak-Halotag-RfA

    Plasmid
    #186377
    Purpose
    Destination vector for the expression of a fluorescent fusion of N-ter Halo tag and a protein of interest into embryos, by mRNA microinjection
    Depositor
    Patrick Lemaire
    Type
    Empty backbone
    Use
    Gateway Cloning
    Available Since
    May 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    186377-plasmid-map-sequence-id-392694

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  35. pDEST-Spe4-RfA-Snaptag-stop

    Plasmid
    #186395
    Purpose
    Destination vector for the expression of a fluorescent fusion of a protein of interest with C-ter Snap tag into embryos, by mRNA microinjection
    Depositor
    Patrick Lemaire
    Type
    Empty backbone
    Use
    Gateway Cloning
    Available Since
    Oct. 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    186395-plasmid-map-sequence-id-373843

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  36. 371-R

    Plasmid
    #97033
    Purpose
    StrepII-tev-yORF
    Depositor
    Chris Jeans
    Type
    Empty backbone
    Tags
    StrepII tag and TEV cleavage site
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    July 11, 2017
    Availability
    Academic Institutions and Nonprofits only
    97033-plasmid-map-sequence-id-182501

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  37. 371-L

    Plasmid
    #97031
    Purpose
    FLAG-tev-yORF
    Depositor
    Chris Jeans
    Type
    Empty backbone
    Tags
    FLAG tag and TEV cleavage site
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    July 11, 2017
    Availability
    Academic Institutions and Nonprofits only
    97031-plasmid-map-sequence-id-182492

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  38. p405ADH1

    Plasmid
    #15966
    Depositor
    Nicolas Buchler, Fred Cross
    Insert
    p405ADH1 (ADH1 Budding Yeast)
    Expression
    Bacterial and Yeast
    Mutation
    ADH1 promoter is between SacI and XbaI restrictio…
    Available Since
    Nov. 2, 2007
    Availability
    Academic Institutions and Nonprofits only
    15966-plasmid-map-sequence-id-8451

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  39. N1-LOC8R

    Plasmid
    #66896
    Purpose
    Leader Of Cox8 Repeated (LOC8R) is a synthetic mitochondrial targeting sequence compatible with any N1 vector. Internal restriction sites enable modular subcloning of 1x, 2x, 3x or 4x presequences.
    Depositor
    Georg Ramm
    Insert
    LOC8R
    Available Since
    July 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    66896-plasmid-map-sequence-id-114924

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  40. pMC6-TEFkFLP(LYS2)

    Plasmid
    #49458
    Purpose
    overespression of Flp step-arrest mutant in yeast, LYS2 selection
    Depositor
    Marc Gartenberg
    Insert
    Flp(H305L) (flp Budding Yeast)
    Expression
    Yeast
    Mutation
    H305L, ‘step-arrest’ mutant,
    Promoter
    TEF1
    Available Since
    Dec. 3, 2013
    Availability
    Academic Institutions and Nonprofits only
    49458-plasmid-map-sequence-id-73670

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  41. ACT1-CUP1 9bp dist

    Plasmid
    #234131
    Purpose
    PCR to add remove nucleotides within intron of ACT1-CUP1 insert then restriction digest and ligation of new insert to backbone
    Depositor
    Aaron Hoskins
    Insert
    ACT1-CUP1
    Expression
    Yeast
    Mutation
    Intron truncation
    Available Since
    April 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    234131-plasmid-map-sequence-id-466522

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  42. ACT1-CUP1 12bp dist

    Plasmid
    #234130
    Purpose
    PCR to add remove nucleotides within intron of ACT1-CUP1 insert then restriction digest and ligation of new insert to backbone
    Depositor
    Aaron Hoskins
    Insert
    ACT1-CUP1
    Expression
    Yeast
    Mutation
    Intron truncation
    Available Since
    April 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    234130-plasmid-map-sequence-id-466519

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  43. ACT1-CUP1 27bp dist

    Plasmid
    #234129
    Purpose
    PCR to add remove nucleotides within intron of ACT1-CUP1 insert then restriction digest and ligation of new insert to backbone
    Depositor
    Aaron Hoskins
    Insert
    ACT1-CUP1
    Expression
    Yeast
    Mutation
    Intron truncation
    Available Since
    April 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    234129-plasmid-map-sequence-id-466539

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  44. ACT1-CUP1 15bp dist

    Plasmid
    #234128
    Purpose
    PCR to add remove nucleotides within intron of ACT1-CUP1 insert then restriction digest and ligation of new insert to backbone
    Depositor
    Aaron Hoskins
    Insert
    ACT1-CUP1
    Expression
    Yeast
    Mutation
    Intron truncation
    Available Since
    April 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    234128-plasmid-map-sequence-id-466529

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  45. ACT1-CUP1 42bp dist

    Plasmid
    #234127
    Purpose
    PCR to add remove nucleotides within intron of ACT1-CUP1 insert then restriction digest and ligation of new insert to backbone
    Depositor
    Aaron Hoskins
    Insert
    ACT1-CUP1
    Expression
    Yeast
    Mutation
    Intron truncation
    Available Since
    April 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    234127-plasmid-map-sequence-id-466531

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  46. ACT1-CUP1 50bp dist

    Plasmid
    #234112
    Purpose
    PCR to add remove nucleotides within intron of ACT1-CUP1 insert then restriction digest and ligation of new insert to backbone
    Depositor
    Aaron Hoskins
    Insert
    ACT1-CUP1
    Expression
    Yeast
    Mutation
    Intron truncation
    Available Since
    April 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    234112-plasmid-map-sequence-id-466054

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  47. ACT1-CUP1 46bp dist

    Plasmid
    #234111
    Purpose
    PCR to add remove nucleotides within intron of ACT1-CUP1 insert then restriction digest and ligation of new insert to backbone
    Depositor
    Aaron Hoskins
    Insert
    ACT1-CUP1
    Expression
    Yeast
    Mutation
    Intron truncation
    Available Since
    April 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    234111-plasmid-map-sequence-id-466051

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  48. 371-T

    Plasmid
    #97035
    Purpose
    His6-Thioredoxin-tev-yORF
    Depositor
    Chris Jeans
    Type
    Empty backbone
    Tags
    His6 tag, TEV cleavage site, and Thioredoxin funs…
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    July 11, 2017
    Availability
    Academic Institutions and Nonprofits only
    97035-plasmid-map-sequence-id-182503

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  49. 371-S

    Plasmid
    #97034
    Purpose
    His6-Sumo-tev-yORF
    Depositor
    Chris Jeans
    Type
    Empty backbone
    Tags
    His6 tag, SUMO domain, and TEV cleavage site
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    July 11, 2017
    Availability
    Academic Institutions and Nonprofits only
    97034-plasmid-map-sequence-id-182498

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  50. 371-O

    Plasmid
    #97032
    Purpose
    His6-Mocr-tev-yORF
    Depositor
    Chris Jeans
    Type
    Empty backbone
    Tags
    His6 tag, TEV cleavage site, and mOCR fusion
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    July 11, 2017
    Availability
    Academic Institutions and Nonprofits only
    97032-plasmid-map-sequence-id-182497

    Enlarge

Showing: 1701 - 1750 of 79968 results