We narrowed to 75,668 results for: Rest
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Plasmid#17421DepositorInsertMET25 promoter + polylinker + CYC1 terminator (MET17 Budding Yeast)
UseTagsExpressionBacterial and YeastMutationMET25 promoter is between SacI and XbaI restricti…PromoterAvailable sinceMarch 12, 2008AvailabilityAcademic Institutions and Nonprofits only -
pHR-FKBP:mCherry-KRas4Bc30
Plasmid#86633PurposeExpresses mCherry-tagged membrane recruiter constructDepositorInsertFKBP:mCherry-KRas4Bc30
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGGDestSC-ATG
Plasmid#49322PurposeDestination vector for Golden Gate assembly; Few restriction sites for subcloning.DepositorTypeEmpty backboneUseDestination vectorTagsExpressionMutationPromoterAvailable sinceSept. 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pJQ200uc1
Plasmid#78502PurposeSuicide vector for gene replacement, modified MCS to maximise utility of insert restriction sites.DepositorTypeEmpty backboneUseSuicide vector for gene replacementTagsnoneExpressionMutationPromoternoneAvailable sinceAug. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
arrSa
Plasmid#78169Purposezf arrSa in pCRII-TOPODepositorInsertarrSa
UseTagsExpressionMutationPromoterAvailable sinceJune 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGL3 RARE-RFP
Plasmid#183054PurposeFluorescent reporter for Retinoic Acid activityDepositorInsertRFP
UseTagsExpressionMutationPromoterRARE, SV40Available sinceApril 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRL443
Plasmid#70261PurposeShuttle vector suitable for mobilizing plasmids to Anabaena (Nostoc) sp. strain PCC 7120 and other cyanobacterial strains.DepositorTypeEmpty backboneUseTagsExpressionMutationPromoterAvailable sinceFeb. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pIHEU-MCS
Plasmid#58375PurposeUAS vector to express gene of interest in DrosophilaDepositorTypeEmpty backboneUseTagsExpressionInsectMutationPromoterAvailable sinceJan. 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pGEX6P1-N-FLAG
Plasmid#119759PurposeAllows cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag, and a retained N-terminal FLAG tag.DepositorTypeEmpty backboneUseTagsFLAG and GSTExpressionBacterialMutationPromotertac promoterAvailable sinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pSFV3-lacZ
Plasmid#92074PurposeThis is an expression plasmid for production of recombinant genomic SFV RNA encoding beta-galactosidase.DepositorInsertlacZ
UseLacz expression plasmid, for run-off transcriptio…TagsExpressionMutationPromoterAvailable sinceJune 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-N-His
Plasmid#119755PurposeAllows cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag, and a retained N-terminal 10x His tag.DepositorTypeEmpty backboneUseTags10xHis and GSTExpressionBacterialMutationPromotertac promoterAvailable sinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUltra-Plus-H2B-mTagBFP2
Plasmid#161790Purpose3rd generation lentiviral bicistronic vector for mammalian expression of H2B-mTagBFP2 and one gene of interestDepositorInsertH2B-mTagBFP2
UseLentiviral; BicistronicTagsmTagBFP2ExpressionMammalianMutationI174A in the mTagBFP2 protein according to PLoS O…PromoterUbC and UbC promoterAvailable sinceNov. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pNDamMyc
Plasmid#59217PurposeDrosophila DamID vector used to fuse gene of interest to Myc-tagged E.coli Dam.DepositorTypeEmpty backboneUseDamidTagsDam and MycExpressionInsectMutationPromoterDrosophila heat-shock promoterAvailable sinceMay 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-N-Strep2
Plasmid#119758PurposeAllows cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag, and a retained N-terminal Strep-II tag.DepositorTypeEmpty backboneUseTagsGST and Strep-tag II fusion tagExpressionBacterialMutationPromotertac promoterAvailable sinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGPI-SceI
Plasmid#32060PurposeConstruction of unmarked gene deletions. Suicide cloning vector designed to introduce a targeted I-SceI restriction site in the genome of BurkholderiaDepositorTypeEmpty backboneUseMutagenesis plasmidTagsExpressionMutationPromoterAvailable sinceAug. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMycDam
Plasmid#59218PurposeDrosophila DamID vector used to fuse gene of interest to Myc-tagged E.coli DamDepositorTypeEmpty backboneUseDamidTagsDam and MycExpressionInsectMutationPromoterDrosophila heat-shock promoterAvailable sinceMay 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMS2-2
Plasmid#220628PurposePlasmid allows you to place the RNA sequence of interest next to two MS2 coat protein recognition sites.DepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceJune 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
p405MET25
Plasmid#15961DepositorInsertMET25 promoter + polylinker + CYC1 terminator (MET17 Budding Yeast)
UseTagsExpressionBacterial and YeastMutationMET25 promoter is between SacI and XbaI restricti…PromoterAvailable sinceNov. 2, 2007AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-N-Myc
Plasmid#119757PurposeAllows cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag, and a retained N-terminal Myc tag.DepositorTypeEmpty backboneUseTagsGST and MycExpressionBacterialMutationPromotertac promoterAvailable sinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFKBPmCherry-Akt2(KD)
Plasmid#86598PurposeExpresses recruitable mCherry-tagged kinase domain of human Akt2(WT)DepositorInsertFKBP-mCherry-Akt2(KD)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHaloTag-PS(RA)
Plasmid#86627PurposeExpresses Akt pseudosubstrate (P-3 RA) mutant tagged with a HaloTagDepositorInsertHaloTag-PS(RA)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
H-ACNF-H
Plasmid#15515DepositorInsertH element
UseMouse TargetingTagsExpressionMutationTargeting vector to delete the 2,119 nt sequence,…PromoterAvailable sinceAug. 24, 2007AvailabilityAcademic Institutions and Nonprofits only -
pBlue-hFX-HA(AscI)
Plasmid#15404DepositorInserthuman frataxin cDNA (FXN Human)
UseTagsAU1 and HAExpressionBacterialMutation700 bp insert can be released by digestion with r…PromoterAvailable sinceAug. 17, 2007AvailabilityAcademic Institutions and Nonprofits only -
pDL1530
Plasmid#226636PurposeThis plasmid is for the induced expression of nrsB (formerly known as ORF229) from ICP1_2001.DepositorInsertnrsB (ORF 229)
UseTagsExpressionMutationPromoterAvailable sinceNov. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
POC1400
Plasmid#213121PurposeTesting the activity of BpiI-associated switch methylasesDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
POC1401
Plasmid#213122PurposeTesting the activity of LguI-associated switch methylasesDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
POC1399
Plasmid#213120PurposeTesting the activity of BsaI-associated switch methylasesDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGL3 RARE-d2EGFP
Plasmid#183055PurposeDestabilized EGFP fluorescent reporter for Retinoic Acid activityDepositorInsertd2EGFP
UseTagsExpressionMutationPromoterRARE, SV40Available sinceApril 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSFV3
Plasmid#92072PurposeEmpty expression plasmid for production of recombinant genomic SFV RNA. Co-transfection with RNA from pSFV-Helper1 or pSFV-Helper2 will produce recombinant virus like particles.DepositorTypeEmpty backboneUseEmpty expression plasmid, for run-off transcripti…TagsExpressionMutationPromoterAvailable sinceJune 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pABY-T2
Plasmid#201765PurposeFor C-terminal tagging of a protein-of-interest with the ALFA tag using S2/S3 primers in yeastDepositorInsertALFA tag
UseTagsExpressionYeastMutationPromoterAvailable sinceAug. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pIII/MS2-2
Plasmid#220630PurposeFor expressing an RNA containing your sequence of interest adjacent to two MS2 sites from an RNA polymerase III promoter.DepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceOct. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBIS-GALkFLP(Trp1)
Plasmid#49459Purposeoverespression of Flp step-arrest mutant in yeast, TRP1 selectionDepositorInsertFlp(H305L) (flp Budding Yeast)
UseTagsExpressionYeastMutationH305L, ‘step-arrest’ mutant,PromoterGAL10Available sinceNov. 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
pABY-T1
Plasmid#201764PurposeFor C-terminal tagging of a protein-of-interest with the ALFA tag using S2/S3 primers in yeastDepositorInsertALFA tag
UseTagsExpressionYeastMutationPromoterAvailable sinceAug. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUPD2-HR1b-GFP-L (B3-B4)
Plasmid#211853PurposeE3-DART degron casette for N-terminal fusion to protein of interest using GoldenbraidDepositorInsertHR1b
UseSynthetic BiologyTagsGFPExpressionMutationPromoterAvailable sinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCL20C-ddIPpoI
Plasmid#49053PurposeExpresses the restriction enzyme IPpoI under the control of Shield1DepositorInsertDD-HA-ER-IPpoI
UseLentiviralTagsExpressionMutationPromoterAvailable sinceDec. 17, 2013AvailabilityAcademic Institutions and Nonprofits only -
BsaI-tagRFP-BsaI-pUC-kan
Plasmid#170771Purposetest plasmid for Golden Gate assembly, encodes fluorescent red proteinDepositorInserttagRFP
UseTagsExpressionMutationPromoternoneAvailable sinceFeb. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDL1403
Plasmid#226635PurposeThis plasmid induces natural competence in classical biotype Vibrio cholerae and expresses the genes tfox and qstR.DepositorInsertstfox
qstR
UseTagsExpressionMutationPromoterAvailable sinceJan. 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pABY-T3
Plasmid#201766PurposeFor C-terminal tagging of a protein-of-interest with the ALFA tag using S2/S3 primers in yeastDepositorInsertALFA tag
UseTagsExpressionYeastMutationPromoterAvailable sinceAug. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pABY-T4
Plasmid#201767PurposeFor C-terminal tagging of a protein-of-interest with the ALFA tag using S2/S3 primers in yeastDepositorInsertALFA tag
UseTagsExpressionYeastMutationPromoterAvailable sinceAug. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
Halo-TMD
Plasmid#195347PurposeFluorescent labeling for cell membranesDepositorInsertHaloTag
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFGL815
Plasmid#52322PurposeA vector backbone for ATMT (Agrobacterium tumerfaciens-mediated transformation). There is a 69-bp MCS (multiple cloning site) which contains 12 unique restriction sites.DepositorTypeEmpty backboneUseAgrobacteriumTagsExpressionBacterialMutationPromoterAvailable sinceJuly 8, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMtBS
Plasmid#32226DepositorInsertMCV Small t antigen
UseTagsExpressionMammalianMutationSplice site was silently mutated, and a restricti…PromoterAvailable sinceOct. 19, 2011AvailabilityAcademic Institutions and Nonprofits only -
pAllet
Plasmid#202560PurposeE. coli cloning vector with large MCS (50+ unique restriction sites)DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
DinoIII
Plasmid#128100PurposeDinoflagellate expression vector backbone. XbaI and BglII restriction sites for gene insertion.DepositorInsertRNA complex & upstream/downstream of LHC
UseTagsExpressionBacterialMutationPromoterInserts are potential promotersAvailable sinceApril 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pID3.01B
Plasmid#22720DepositorInsertunc-119 rescuing fragment / pie-1promoter-GFP- gateway destination cassette B -pie-1 3’
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 21, 2010AvailabilityAcademic Institutions and Nonprofits only -
pcDNA 3.1(-) TRIM12A
Plasmid#87061PurposeMurine homologues of human Trim5DepositorInsertTRIM12A (Trim12a Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA 3.1(-) TRIM12C
Plasmid#87080PurposeMurine homologues of human Trim5DepositorInsertTRIM12C (Trim12c Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCBLggCDS
Plasmid#199737PurposeGolden Gate entry vector for gene of interest cloningDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceApril 27, 2023AvailabilityAcademic Institutions and Nonprofits only