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We narrowed to 5,049 results for: golden gate

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Showing: 1701 - 1750 of 5049 results

  1. MUT_S1-H1

    Plasmid
    #218394
    Purpose
    3-exon minigene, with SRSF1 motif-rich middle exon, hnRNPA1 motif-rich region in second intron, and AG>GG mutation in final 3' splice site
    Depositor
    Oded Regev
    Insert
    Modified CHO DHFR minigene
    Expression
    Mammalian
    Promoter
    tetracycline-responsive promoter
    Available Since
    May 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    218394-plasmid-map-sequence-id-433909

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  2. PB_gRNA_S.pyogenes_scaffold_BB

    Plasmid
    #226429
    Purpose
    PiggyBac transposon vector backbone for cloning of sgRNA compatible with S.pyogenes dCas9 enzyme. BbsI Golden Gate site upstream of the scaffold for protospacer insertion. Puromycin selection marker.
    Depositor
    Alon Goren
    Type
    Empty backbone
    Use
    CRISPR; Piggybac transposon
    Expression
    Mammalian
    Promoter
    U6
    Available Since
    Sept. 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    226429-plasmid-map-sequence-id-458099

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  3. pAIP4_2WWE

    Plasmid
    #234210
    Purpose
    Heterologous protein expression of PI3K-C2-gamma in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    2WWE (PIK3C2G Human)
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234210-plasmid-map-sequence-id-464175

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  5. pTwist-Kan-HC-L0-TaBSS1-5PU

    Plasmid
    #242007
    Purpose
    L0 Pro+5U Golden Gate part of 5'UTR, core promoter, and upstream regulatory regions of Triticum aestivum MANY-NODED DWARF 1 (MND1) B-subgenome homoeolog (TraesCS7B02G413900)
    Depositor
    Cristobal Uauy
    Insert
    TaBSS1-5PU
    Use
    Synthetic Biology
    Expression
    Plant
    Available Since
    March 30, 2026
    Availability
    Academic Institutions and Nonprofits only
    242007-plasmid-map-sequence-id-504528

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  6. pMB.MBP.JARID2

    Plasmid
    #247018
    Purpose
    Expression of MBP-JARID2 in insect cells, MBP tags are C3-cleavable (PreScission Protease)
    Depositor
    Chen Davidovich
    Insert
    JARID2 (JARID2 Human)
    Tags
    MBP
    Expression
    Insect
    Available Since
    Nov. 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    247018-plasmid-map-sequence-id-493374

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  7. pMV_AA100

    Plasmid
    #245322
    Purpose
    Cas9 CRISPRko all-in-one positive control; targets CD55
    Depositor
    John Doench, David Root
    Insert
    Cas9, sgRNA CD55 (CD55 Synthetic)
    Use
    Lentiviral
    Mutation
    None
    Available Since
    Oct. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    245322-plasmid-map-sequence-id-488050

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  8. pMV_AA104

    Plasmid
    #245326
    Purpose
    Cas9 CRISPRa positive control guide; targets CD4
    Depositor
    John Doench, David Root
    Insert
    sgRNA CD4 (CD4 Synthetic)
    Use
    Lentiviral
    Mutation
    None
    Available Since
    Oct. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    245326-plasmid-map-sequence-id-488034

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  9. pTwist-Kan-HC-L0-TaBSS1-3D

    Plasmid
    #242008
    Purpose
    L0 3U* Golden Gate part of 3' UTR and downstream regulatory regions of Triticum aestivum MANY-NODED DWARF 1 (MND1) B-subgenome homoeolog (TraesCS7B02G413900)
    Depositor
    Cristobal Uauy
    Insert
    TaBSS1-3D
    Use
    Synthetic Biology
    Expression
    Plant
    Available Since
    Oct. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    242008-plasmid-map-sequence-id-487882

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  10. pAttBFNeo

    Plasmid
    #244923
    Purpose
    attB Neo Resistant plasmid for RMI targeting an attP site
    Depositor
    Michael Nonet
    Article
    Nonet et al bioRxiv 2024.03.01.583017
    Type
    Empty backbone
    Use
    Phic31 recombinase mediated insertion (rmi)
    Expression
    Worm
    Promoter
    none
    Available Since
    Sept. 8, 2025
    Availability
    Academic Institutions and Nonprofits only
    244923-plasmid-map-sequence-id-485479

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  11. pAttBFNeo2

    Plasmid
    #244924
    Purpose
    attB Neo Resistant plasmid for RMI targeting an attP site
    Depositor
    Michael Nonet
    Article
    Nonet et al bioRxiv 2024.03.01.583017
    Type
    Empty backbone
    Use
    Phic31 recombinase mediated insertion (rmi)
    Expression
    Worm
    Promoter
    none
    Available Since
    Sept. 8, 2025
    Availability
    Academic Institutions and Nonprofits only
    244924-plasmid-map-sequence-id-485477

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  12. pAttPF3Neo

    Plasmid
    #244930
    Purpose
    attP Neo Resistant plasmid for RMI targeting an attB site
    Depositor
    Michael Nonet
    Article
    Nonet et al bioRxiv 2024.03.01.583017
    Type
    Empty backbone
    Use
    Phic31 recombinase mediated insertion (rmi)
    Expression
    Worm
    Promoter
    none
    Available Since
    Sept. 8, 2025
    Availability
    Academic Institutions and Nonprofits only
    244930-plasmid-map-sequence-id-485475

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  14. pSG1016-2xBsaI-SaKKH_P2A_EGFP

    Plasmid
    #239461
    Purpose
    Codon-optimized S. aureus KKH Cas9. 2x BsaI sites for easy cloning of varying deaminases. EGFP can serve as a transfection marker.
    Depositor
    Alexis Komor
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    EGFP
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    June 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    239461-plasmid-map-sequence-id-476832

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  16. pAIP4_1T3Y

    Plasmid
    #234228
    Purpose
    Heterologous protein expression of coactosin-like protein in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    1T3Y (COTL1 Human)
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234228-plasmid-map-sequence-id-464193

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  17. pAIP4_3TOW

    Plasmid
    #234201
    Purpose
    Heterologous protein expression of multivesicular body subunit 12B (ESCRT-I complex subunit MVB12B) in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    3TOW (MVB12B Human)
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234201-plasmid-map-sequence-id-464166

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  18. pAIP4_1KQX

    Plasmid
    #234181
    Purpose
    Heterologous protein expression of cellular retinol-binding protein type II in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    1KQX (rbp2a Zebrafish)
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234181-plasmid-map-sequence-id-464146

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  19. pAIP4_1AKO

    Plasmid
    #234162
    Purpose
    Heterologous protein expression of exodeoxyribonuclease III (Exonuclease III) in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    1AKO (xthA Escherichia coli K-12)
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234162-plasmid-map-sequence-id-464128

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  20. pAIP4_4NFB

    Plasmid
    #234161
    Purpose
    Heterologous protein expression of paired immunoglobulin-like type 2 receptor alpha in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    4NFB (PILRA Human)
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234161-plasmid-map-sequence-id-464127

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  22. L1T11 - T2BP/p35S (BFP, mCherry, NeonGreen)

    Plasmid
    #219688
    Purpose
    Level 1 History-dependent target, switch Bxb1 then PhiC31, p35S promoter, and output: mtagBFP2 then mCherry then NeonGreen (for transformation, Kan plant resistance, Kan bacteria resistance).
    Depositor
    Jennifer Nemhauser
    Insert
    T2BP/p35S (BFP, mCherry, NeonGreen)
    Expression
    Plant
    Available Since
    July 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    219688-plasmid-map-sequence-id-435723

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  23. MUT_S7-H1

    Plasmid
    #218396
    Purpose
    3-exon minigene, with SRSF7 motif-rich middle exon, hnRNPA1 motif-rich region in second intron, and AG>GG mutation in final 3' splice site
    Depositor
    Oded Regev
    Insert
    Modified CHO DHFR minigene
    Expression
    Mammalian
    Promoter
    tetracycline-responsive promoter
    Available Since
    May 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    218396-plasmid-map-sequence-id-433914

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  26. pRDB_248

    Plasmid
    #245335
    Purpose
    Cas12a CRISPRko positive control guide; targets CD63
    Depositor
    John Doench, David Root
    Insert
    sgRNA CD63 (CD63 Synthetic)
    Use
    Lentiviral
    Mutation
    None
    Available Since
    Oct. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    245335-plasmid-map-sequence-id-488021

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  28. pMV_AA101

    Plasmid
    #245323
    Purpose
    Cas9 CRISPRa positive control guide; targets CD274
    Depositor
    John Doench, David Root
    Insert
    sgRNA CD274 (CD274 Synthetic)
    Use
    Lentiviral
    Mutation
    None
    Available Since
    Oct. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    245323-plasmid-map-sequence-id-488047

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  29. pRDB_510

    Plasmid
    #245337
    Purpose
    Cas9 CBE positive control guide; targets CD274
    Depositor
    John Doench, David Root
    Insert
    sgRNA CD274 (CD274 Synthetic)
    Use
    Lentiviral
    Mutation
    None
    Available Since
    Oct. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    245337-plasmid-map-sequence-id-488041

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  30. pRDB_509

    Plasmid
    #245336
    Purpose
    Cas9 ABE positive control guide; targets CD274
    Depositor
    John Doench, David Root
    Insert
    sgRNA CD274 (CD274 Synthetic)
    Use
    Lentiviral
    Mutation
    None
    Available Since
    Oct. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    245336-plasmid-map-sequence-id-488033

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  31. pTwist-Kan-HC-L0-TaMOFB1(int)

    Plasmid
    #242005
    Purpose
    L0 CDS1 Golden Gate part of the pre-mRNA (excluding UTRs) of Triticum aestivum MORE FLORET 1 (MOF-1) / MULTI-FLORET SPIKELET 2 (MFS-2) B-subgenome homoeolog (TraesCS2B02G420900)
    Depositor
    Cristobal Uauy
    Insert
    TaMOF-B1_with_introns
    Use
    Synthetic Biology
    Expression
    Plant
    Available Since
    Oct. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    242005-plasmid-map-sequence-id-487883

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  32. pAttBFGNeo2

    Plasmid
    #244928
    Purpose
    attB Neo Resistant mNeonGreen marked plasmid for RMI targeting an attP site
    Depositor
    Michael Nonet
    Article
    Nonet et al bioRxiv 2024.03.01.583017
    Type
    Empty backbone
    Use
    Phic31 recombinase mediated insertion vector
    Expression
    Worm
    Promoter
    none
    Available Since
    Sept. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    244928-plasmid-map-sequence-id-486011

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  33. pAttBFGNeo

    Plasmid
    #244925
    Purpose
    attB Neo Resistant mNeonGreen marked plasmid for RMI targeting an attP site
    Depositor
    Michael Nonet
    Article
    Nonet et al bioRxiv 2024.03.01.583017
    Type
    Empty backbone
    Use
    Phic31 recombinase mediated insertion (rmi)
    Expression
    Worm
    Promoter
    none
    Available Since
    Sept. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    244925-plasmid-map-sequence-id-486006

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  34. pAIP4_3CX2

    Plasmid
    #234221
    Purpose
    Heterologous protein expression of myosin-binding protein C, cardiac-type in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    3CX2 (MYBPC3 Human)
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234221-plasmid-map-sequence-id-464186

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  35. pAIP4_4ACJ

    Plasmid
    #234212
    Purpose
    Heterologous protein expression of Wufb25h12 in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    4ACJ (oxr1a Zebrafish)
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234212-plasmid-map-sequence-id-464177

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  36. pAIP4_1EYH

    Plasmid
    #234159
    Purpose
    Heterologous protein expression of epsin-1 (EPS-15-interacting protein 1) in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    1EYH (Epn1 Rat)
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234159-plasmid-map-sequence-id-464125

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  37. MUT_S1-spacer-H1

    Plasmid
    #218399
    Purpose
    3-exon minigene, with SRSF1 motif-rich middle exon, hnRNPA1 motif-rich region in second intron following a neutral spacer, and AG>GG mutation in final 3' splice site
    Depositor
    Oded Regev
    Insert
    Modified CHO DHFR minigene
    Expression
    Mammalian
    Promoter
    tetracycline-responsive promoter
    Available Since
    June 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    218399-plasmid-map-sequence-id-436404

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  38. IBMc460

    Plasmid
    #161940
    Purpose
    Carries Golden Gate substitution insert for IBM with gp41-1 intein. PPhlF as C-lobe promoter
    Depositor
    Baojun Wang
    Article
    Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:
    Insert
    pSB1A3-BbsI-GY-gp-41-1N-cat-PPhlF-RiboJ-B32-gp41-1C-SS-SapI
    Expression
    Bacterial
    Promoter
    PPhlF
    Available Since
    March 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    161940-plasmid-map-sequence-id-329041

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  39. IBMc180

    Plasmid
    #161942
    Purpose
    Carries Golden Gate substitution insert for acVHH-assisted bisection mapping
    Depositor
    Baojun Wang
    Article
    Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:
    Insert
    pSB1A3-BbsI-10aa-acVHH-T-cat-PBAD(SapI)-RiboJ-BCD22-acVHH-10aa-SapI
    Expression
    Bacterial
    Promoter
    PBAD
    Available Since
    March 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    161942-plasmid-map-sequence-id-329042

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  41. pAIP4_3RZY

    Plasmid
    #234156
    Purpose
    Heterologous protein expression of fatty acid-binding protein, adipocyte (ALBP) in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    3RZY (FABP4 Human)
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234156-plasmid-map-sequence-id-464122

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  44. L1PB26- pAHP6--PhiC31-NLS-tUBQ1 + pGATA23--Bxb1-SAUR-tUBQ10

    Plasmid
    #219701
    Purpose
    Level 1 PhiC31 and Bxb1 integrase construct for tracking of lateral root development: pAHP6 promoter for PhiC31 and pGATA23 promoter for Bxb1, NLS tag for PhiC31 and RNA degradation tag for Bxb1
    Depositor
    Jennifer Nemhauser
    Insert
    pAHP6::PhiC31-NLS:tUBQ1 + pGATA23::Bxb1-SAUR:tUBQ10
    Expression
    Plant
    Available Since
    Oct. 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    219701-plasmid-map-sequence-id-435731

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  45. pAGM4723:Ble:Cas9

    Plasmid
    #112208
    Purpose
    Golden Gate Level 2 construct encoding Cas9-YFP, Zeocin resistance.
    Depositor
    Thomas Mock
    Inserts
    Cas9
    SheBle
    Use
    CRISPR; Diatom, phaeodactylum
    Tags
    Phaeodactylum fcp promoter and terminator and YFP
    Mutation
    Phaeodactylum Fcp promoter
    Promoter
    Phaeodactylum Fcp
    Available Since
    July 31, 2018
    Availability
    Academic Institutions and Nonprofits only
    112208-plasmid-map-sequence-id-217248

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  46. pJF16

    Plasmid
    #253747
    Purpose
    Encodes GFP_AID* and pALD6-OsTIR1(F74G) expression cassettes. The OsTIR1(F74G) coding sequence is codon optimized for S. cerevisiae.
    Depositor
    Matthias Heinemann
    Inserts
    GFP_AID*
    OsTIR1
    Tags
    mAID*-tag
    Expression
    Yeast
    Mutation
    F74G
    Promoter
    ALD6 and TEF1
    Available Since
    April 27, 2026
    Availability
    Academic Institutions and Nonprofits only
    253747-plasmid-map-sequence-id-507847

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  47. pJF34

    Plasmid
    #253752
    Purpose
    Encodes GFP_AID* and pADH1-OsTIR1(F74G) expression cassettes. The OsTIR1(F74G) coding sequence is not codon optimized for S. cerevisiae.
    Depositor
    Matthias Heinemann
    Inserts
    GFP_AID*
    OsTIR1 (non codon optimized)
    Tags
    mAID*-tag
    Expression
    Yeast
    Mutation
    F74G
    Promoter
    ADH1 and TEF1
    Available Since
    April 27, 2026
    Availability
    Academic Institutions and Nonprofits only
    253752-plasmid-map-sequence-id-507841

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  48. pJF15

    Plasmid
    #253746
    Purpose
    Encodes GFP_AID* and pRPL18B-OsTIR1(F74G) expression cassettes. The OsTIR1(F74G) coding sequence is codon optimized for S. cerevisiae.
    Depositor
    Matthias Heinemann
    Inserts
    GFP_AID*
    OsTIR1
    Tags
    mAID*-tag
    Expression
    Yeast
    Mutation
    F74G
    Promoter
    RPL18B and TEF1
    Available Since
    April 27, 2026
    Availability
    Academic Institutions and Nonprofits only
    253746-plasmid-map-sequence-id-507840

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  49. pJF17

    Plasmid
    #253748
    Purpose
    Encodes GFP_AID* and pRNR2-OsTIR1(F74G) expression cassettes. The OsTIR1(F74G) coding sequence is codon optimized for S. cerevisiae.
    Depositor
    Matthias Heinemann
    Inserts
    GFP_AID*
    OsTIR1
    Tags
    mAID*-tag
    Expression
    Yeast
    Mutation
    F74G
    Promoter
    RNR2 and TEF1
    Available Since
    April 27, 2026
    Availability
    Academic Institutions and Nonprofits only
    253748-plasmid-map-sequence-id-507839

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  50. pJF18

    Plasmid
    #253749
    Purpose
    Encodes GFP_AID* and pREV1-OsTIR1(F74G) expression cassettes. The OsTIR1(F74G) coding sequence is codon optimized for S. cerevisiae.
    Depositor
    Matthias Heinemann
    Inserts
    GFP_AID*
    OsTIR1
    Tags
    mAID*-tag
    Expression
    Yeast
    Mutation
    F74G
    Promoter
    REV1 and TEF1
    Available Since
    April 27, 2026
    Availability
    Academic Institutions and Nonprofits only
    253749-plasmid-map-sequence-id-507834

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Showing: 1701 - 1750 of 5049 results