We narrowed to 4,045 results for: golden gate

Showing: 1701 - 1750 of 4045 results

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pGGD000

Plasmid

#48859

Purpose

Empty entry vector for creating GreenGate C-terminal tag modules.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Type

Empty backbone

Use

Golden gate compatible cloning vector

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pYY62-P1int-TP901-GFPdropout

Plasmid

#235773

Purpose

Plasmid used for constructing donor DNA for TP901-compatible p1 integration. This plasmid is a sfGFP dropout plasmid where sfGFP can be replaced by GOIs using Golden Gate Assembly.

Depositor

Chang Liu

Article

Pisera et al bioRxiv [Preprint]. 2024 Nov 30:2024.11.

Type

Empty backbone

Available Since

May 5, 2025

Availability

Academic Institutions and Nonprofits only

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BCJJ163

Plasmid

#117512

Purpose

AtRPS5a promoter Golden Gate Level 0

Depositor

Jonathan D Jones

Article

Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

Insert

BsaI:GGAG:pRPS5a:AATG:BsaI

Expression

Plant

Promoter

AtRPS5a

Available Since

Nov. 1, 2018

Availability

Academic Institutions and Nonprofits only

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LBJJ491

Plasmid

#117513

Purpose

AtYAO promoter Golden Gate Level 0

Depositor

Jonathan D Jones

Article

Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

Insert

BsaI:GGAG:pYAO:AATG:BsaI

Expression

Plant

Promoter

AtYAO

Available Since

Nov. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pRW402-P1int-BXB1

Plasmid

#235979

Purpose

Plasmid used for constructing donor DNA for BXB1-compatible p1 integration. You can insert your GOIs into this plasmid by using Golden Gate Assembly.

Depositor

Chang Liu

Article

Pisera et al bioRxiv [Preprint]. 2024 Nov 30:2024.11.

Type

Empty backbone

Available Since

April 16, 2025

Availability

Academic Institutions and Nonprofits only

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pJet1.2 sfGFP

Plasmid

#117123

Purpose

C-terminal GFP tag

Depositor

Stephan Gruber

Article

Diebold-Durand et al Methods Mol Biol. 2019;2004:49-61. doi:

Insert

Superfolder GFP

Use

Golden gate donor vector for gene targeting in ba…

Tags

sfGFP

Available Since

Nov. 14, 2018

Availability

Academic Institutions and Nonprofits only

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ZIKV isolate VE_Ganxian2016 NS2B-NS3 protease co-expression of NS2B and NS3 protease domain

Plasmid

#228663

Purpose

Bacterial co-expression of the NS2B and NS3 proteases from Zika virus for crystallization assays in P43 2 2 (PDB:8PN6)

Depositor

ASAP Discovery, Frank von Delft

Insert

Zika virus portion of NS2B protease coexpressed with NS3 protease

Tags

His-GST-TEV

Expression

Bacterial

Available Since

Feb. 18, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pSC142

Plasmid

#122835

Purpose

SUC2 promoter with GreenGate A-B flanking sequences

Depositor

Markus Schmid

Article

Lee et al PLoS One. 2019 Sep 26;14(9):e0222778. do

Insert

SUC2 promoter

Use

Golden gate compatible cloning vector

Available Since

March 26, 2019

Availability

Academic Institutions and Nonprofits only

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pSR1154

Plasmid

#221653

Purpose

Tetracycline sensor v3 for Staphylococcus aureus

Depositor

Lauren Andrews

Article

Rondthaler et al ACS Synth Biol. 2023 Dec 8. doi: 10.1021

Inserts

sfGFP cassette
TetR cassette

Use

Synthetic Biology

Expression

Bacterial

Promoter

PXyl-TetO and PZwf

Available Since

March 5, 2025

Availability

Academic Institutions and Nonprofits only

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pGGA008

Plasmid

#48817

Purpose

Provides the alcA promoter as GreenGate module.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Insert

alcA promoter

Use

Golden gate compatible cloning vector

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pICSL4723-P1

Plasmid

#191786

Purpose

Level P Golden gate accepter

Depositor

Wendy Harwood

Article

Lawrenson et al Methods Mol Biol. 2023;2653:253-271. doi

Type

Empty backbone

Expression

Plant

Available Since

Jan. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pGGC011

Plasmid

#48825

Purpose

Provides alcR as GreenGate coding sequence module.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Insert

alcR

Use

Golden gate compatible cloning vector

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pAIP4_2PPN

Plasmid

#234190

Purpose

Heterologous protein expression of peptidyl-prolyl cis-trans isomerase FKBP1A (PPIase FKBP1A) in Escherichia coli

Depositor

Christopher Bahl

Insert

2PPN (FKBP1A Human)

Tags

10x HisTag-Smt3

Expression

Bacterial

Promoter

T7

Available Since

March 6, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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DVC_pBR322_AF

Plasmid

#217598

Purpose

Destination vector with chloramphenicol resistance and pBR322 origin of replication. Carries A-F CIDAR MoClo overhangs for level 1 construct cloning.

Depositor

Wei Huang

Article

Nielsen et al ACS Synth Biol. 2025 Jan 12. doi: 10.102

Type

Empty backbone

Use

Synthetic Biology

Mutation

None

Available Since

Aug. 19, 2024

Availability

Academic Institutions and Nonprofits only

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DVC_p15A_AF

Plasmid

#217588

Purpose

Destination vector with chloramphenicol resistance and p15A origin of replication. Carries A-F CIDAR MoClo overhangs for level 1 construct cloning.

Depositor

Wei Huang

Article

Nielsen et al ACS Synth Biol. 2025 Jan 12. doi: 10.102

Type

Empty backbone

Use

Synthetic Biology

Mutation

None

Available Since

Aug. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pSF18

Plasmid

#222593

Purpose

BB2 ZWF1 tet-on overexpression cassette

Depositor

Matthias Steiger

Article

Matthias et al PLOS One

Insert

ZWF1

Expression

Bacterial

Mutation

Internal BsaI and BpiI sites removed from insert

Promoter

tet-on

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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DVK_p15A_AE

Plasmid

#217582

Purpose

Destination vector with kanamycin resistance and p15A origin of replication. Carries A-E CIDAR MoClo overhangs for level 1 construct cloning.

Depositor

Wei Huang

Article

Nielsen et al ACS Synth Biol. 2025 Jan 12. doi: 10.102

Type

Empty backbone

Use

Synthetic Biology

Mutation

None

Available Since

Aug. 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
DVK_pBR322_AE

Plasmid

#217592

Purpose

Destination vector with kanamycin resistance and pBR322 origin of replication. Carries A-E CIDAR MoClo overhangs for level 1 construct cloning.

Depositor

Wei Huang

Article

Nielsen et al ACS Synth Biol. 2025 Jan 12. doi: 10.102

Type

Empty backbone

Use

Synthetic Biology

Mutation

None

Available Since

Aug. 6, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET22b-TEVP-cleavableELP

Plasmid

#219402

Purpose

Tobacco etch virus protease-responsive elastin-like polypeptide (ELP)

Depositor

Danielle Mai

Article

Wirtz et al Biomacromolecules. 2024 Jul 9. doi: 10.1

Insert

TEVP-cleavableELP

Tags

6xHis

Expression

Bacterial

Available Since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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pGGA000

Plasmid

#48856

Purpose

Empty entry vector for creating GreenGate promoter modules.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Type

Empty backbone

Use

Golden gate compatible cloning vector

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pGGC025

Plasmid

#48830

Purpose

Provides 3xGFP as GreenGate coding sequence module.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Insert

3xGFP

Use

Golden gate compatible cloning vector

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
DVC_p15A_AE

Plasmid

#217587

Purpose

Destination vector with chloramphenicol resistance and p15A origin of replication. Carries A-E CIDAR MoClo overhangs for level 1 construct cloning.

Depositor

Wei Huang

Article

Nielsen et al ACS Synth Biol. 2025 Jan 12. doi: 10.102

Type

Empty backbone

Use

Synthetic Biology

Mutation

None

Available Since

April 10, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
DVC_p15A_EF

Plasmid

#217589

Purpose

Destination vector with chloramphenicol resistance and p15A origin of replication. Carries E-F CIDAR MoClo overhangs for level 1 construct cloning.

Depositor

Wei Huang

Article

Nielsen et al ACS Synth Biol. 2025 Jan 12. doi: 10.102

Type

Empty backbone

Use

Synthetic Biology

Mutation

None

Available Since

Sept. 11, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
DVK_p15A_AF

Plasmid

#217583

Purpose

Destination vector with kanamycin resistance and p15A origin of replication. Carries A-F CIDAR MoClo overhangs for level 1 construct cloning.

Depositor

Wei Huang

Article

Nielsen et al ACS Synth Biol. 2025 Jan 12. doi: 10.102

Type

Empty backbone

Use

Synthetic Biology

Mutation

None

Available Since

Aug. 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
DVA_p15A_AF

Plasmid

#217604

Purpose

Destination vector with ampicillin resistance and p15A origin of replication. Carries A-F CIDAR MoClo overhangs for level 0 or 2 construct cloning.

Depositor

Wei Huang

Article

Nielsen et al ACS Synth Biol. 2025 Jan 12. doi: 10.102

Type

Empty backbone

Use

Synthetic Biology

Mutation

None

Available Since

Aug. 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pGGC000

Plasmid

#48858

Purpose

Empty entry vector for creating GreenGate coding sequence modules.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Type

Empty backbone

Use

Golden gate compatible cloning vector

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pTALYM3

Plasmid

#47874

Purpose

Destination plasmid with mClover for Golden Gate reaction of TALE assembly

Depositor

Maria-Elena Torres-Padilla

Article

Miyanari et al Nat Struct Mol Biol. 2013 Oct 6. doi: 10

Type

Empty backbone

Use

TALEN

Tags

mClover

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 10, 2013

Availability

Academic Institutions and Nonprofits only

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pGGF000

Plasmid

#48861

Purpose

Empty entry vector for creating GreenGate plant resistance cassette modules.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Type

Empty backbone

Use

Golden gate compatible cloning vector

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pBP_sgRNA

Plasmid

#190128

Purpose

Level 0 MoClo plasmid for Golden Gate cloning of sgRNAs

Depositor

Veronica Godoy-Carter

Article

Brychcy et al ACS Synth Biol. 2023 Aug 16. doi: 10.102

Type

Empty backbone

Use

CRISPR

Available Since

Aug. 24, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pBTK1051

Plasmid

#209541

Purpose

BTK Type 5 homology arm for Golden Gate assembly

Depositor

Jeffrey Barrick

Article

Lariviere et al bioRxiv 2023.09.19.558440

Insert

Recombination into the S. alvi wkB2 genome within the SALWKB2_RS11215/SALWKB2_RS11220 intergenic region

Use

Synthetic Biology

Available Since

Feb. 2, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
DVC_p15A_FG

Plasmid

#217590

Purpose

Destination vector with chloramphenicol resistance and p15A origin of replication. Carries F-G CIDAR MoClo overhangs for level 1 construct cloning.

Depositor

Wei Huang

Article

Nielsen et al ACS Synth Biol. 2025 Jan 12. doi: 10.102

Type

Empty backbone

Use

Synthetic Biology

Mutation

None

Available Since

Aug. 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pGGC026

Plasmid

#48831

Purpose

Provides 3xmCherry as GreenGate coding sequence module.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Insert

3xmCherry

Use

Golden gate compatible cloning vector

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pGGG002

Plasmid

#48851

Purpose

Provides an H-A adapter as GreenGate module.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Insert

H-A adapter - short

Use

Golden gate compatible cloning vector

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pGGB000

Plasmid

#48857

Purpose

Empty entry vector for creating GreenGate N-terminal tag modules.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Type

Empty backbone

Use

Golden gate compatible cloning vector

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pDG058

Plasmid

#122840

Purpose

SRDX repressor domain with GreenGate D-E flanking sequences

Depositor

Markus Schmid

Article

Lee et al PLoS One. 2019 Sep 26;14(9):e0222778. do

Insert

SRDX transcriptional repressor domain

Use

Golden gate compatible cloning vector

Available Since

March 26, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pMH185

Plasmid

#122839

Purpose

VP64 activator domain with GreenGate D-E flanking sequences

Depositor

Markus Schmid

Article

Lee et al PLoS One. 2019 Sep 26;14(9):e0222778. do

Insert

VP64 transcriptional activator domain

Use

Golden gate compatible cloning vector

Available Since

March 26, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pGGB001

Plasmid

#48819

Purpose

Provides mCherry:linker as GreenGate N-terminal tag module.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Insert

mCherry:Linker

Use

Golden gate compatible cloning vector

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pGGM000

Plasmid

#48864

Purpose

Empty entry vector for creating GreenGate supermodules w/o plant resistance cassette.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Type

Empty backbone

Use

Golden gate compatible cloning vector

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pGGN000

Plasmid

#48865

Purpose

Empty entry vector for creating GreenGate supermodules with plant resistance cassette.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Type

Empty backbone

Use

Golden gate compatible cloning vector

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pGGC012

Plasmid

#48826

Purpose

Provides GFP:NLS as GreenGate coding sequence module.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Insert

GFP:NLS

Use

Golden gate compatible cloning vector

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pGGE000

Plasmid

#48860

Purpose

Empty entry vector for creating GreenGate terminator modules.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Type

Empty backbone

Use

Golden gate compatible cloning vector

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pEF003

Plasmid

#122838

Purpose

RBCS terminator with GreenGate C-D flanking sequences

Depositor

Markus Schmid

Article

Lee et al PLoS One. 2019 Sep 26;14(9):e0222778. do

Insert

RBCS terminator

Use

Golden gate compatible cloning vector

Available Since

March 26, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
GGDestX1-Amp

Plasmid

#157652

Purpose

Golden Gate assembly and cell-free (bacterial) expression of fusion-proteins

Depositor

Karmella Haynes

Article

Haynes et al Methods Mol Biol. 2023;2599:191-214. doi

Type

Empty backbone

Use

Cell-free expression (e. coli sigma-70)

Expression

Bacterial

Promoter

OR2-OR1-Pr

Available Since

Feb. 8, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pJet1.2 sivC

Plasmid

#117131

Purpose

smc downstream homology region

Depositor

Stephan Gruber

Article

Diebold-Durand et al Methods Mol Biol. 2019;2004:49-61. doi:

Insert

sivC

Use

Golden gate donor vector for gene targeting in ba…

Available Since

Nov. 14, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pYPQ132D-RZ-Lb

Plasmid

#134348

Purpose

Golden Gate entry vector; empty vector to clone the 2nd LbCas12a crRNA under OsU3 promoter

Depositor

Yiping Qi

Article

Li et al Plant Biotechnol J. 2019 Nov 15. doi: 10

Type

Empty backbone

Use

CRISPR

Available Since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pGGE005

Plasmid

#48855

Purpose

Provides the At1g76110 terminator as GreenGate module.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Insert

At1g76110 terminator

Use

Golden gate compatible cloning vector

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pGGC051

Plasmid

#48832

Purpose

Provides GUS as GreenGate coding sequence module.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Insert

GUS

Use

Golden gate compatible cloning vector

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
LBJJ433

Plasmid

#117507

Purpose

AtEC1.2 promoter Golden Gate Level 0

Depositor

Jonathan D Jones

Article

Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

Insert

BsaI:GGAG:pEC1.2:AATG:BsaI

Expression

Plant

Available Since

Oct. 31, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pGGC015

Plasmid

#48828

Purpose

Provides mCherry as GreenGate coding sequence module.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Insert

mCherry

Use

Golden gate compatible cloning vector

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
cwby0082

Plasmid

#247432

Purpose

GGA cloning AGGA-H6-GS-promoter-ccdb-TTCC-SNAC-6xhis

Depositor

David Baker

Article

Qian et al bioRxiv 2025.08.05.668824; doi: https://

Insert

ccdb

Expression

Bacterial

Promoter

T7

Available Since

Nov. 25, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge

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We narrowed to 4,045 results for: golden gate