We narrowed to 4,940 results for: MLS

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pL-rpb1-D260N

Plasmid

#10985

Depositor

Jeff Strathern

Article

Malagon et al Genetics. 2006 Apr . 172(4):2201-9.

Insert

rpb1-D260N (RPO21 Budding Yeast)

Expression

Bacterial and Yeast

Mutation

Mutation 778 G to A in the RPB1 ORF resulting i…

Available Since

Dec. 16, 2005

Availability

Academic Institutions and Nonprofits only
pL-rpb1-D261N

Plasmid

#10986

Depositor

Jeff Strathern

Article

Malagon et al Genetics. 2006 Apr . 172(4):2201-9.

Insert

rpb1-D261N (RPO21 Budding Yeast)

Expression

Bacterial and Yeast

Mutation

Mutation 781 G to A in the RPB1 ORF resulting i…

Available Since

Dec. 16, 2005

Availability

Academic Institutions and Nonprofits only
pL-rpb1-R320C

Plasmid

#10987

Depositor

Jeff Strathern

Article

Malagon et al Genetics. 2006 Apr . 172(4):2201-9.

Insert

rpb1-R320C (RPO21 Budding Yeast)

Expression

Bacterial and Yeast

Mutation

Mutation 958 C to T in the RPB1 ORF resulting i…

Available Since

Dec. 16, 2005

Availability

Academic Institutions and Nonprofits only
pL-rpb1-E1230K

Plasmid

#10990

Depositor

Jeff Strathern

Article

Malagon et al Genetics. 2006 Apr . 172(4):2201-9.

Insert

rpb1-E1230K (RPO21 Budding Yeast)

Expression

Bacterial and Yeast

Mutation

Mutation 3688 G to A in the RPB1 ORF resulting in…

Available Since

Dec. 16, 2005

Availability

Academic Institutions and Nonprofits only
pLVX-TetOne-Puro-EcSTH-FLAG

Plasmid

#218628

Purpose

This plasmid contains human codon optimized sequence of EcSTH which is a soluble transhydrogenase from E. coli that can be used to increase NADH/NAD+ ratio in mammalian cells.

Depositor

Valentin Cracan

Article

Pan et al Nat Chem Biol. 2023 Oct 26. doi: 10.1038

Insert

EcSTH

Use

Lentiviral and Synthetic Biology

Tags

FLAG

Expression

Mammalian

Available Since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

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INCTbiosyn-pUB-HspINTphiC31

Plasmid

#127518

Purpose

Plasmid encodes H. sapiens codon optimized Integrase phiC31.

Depositor

Martin Bonamino

Article

Gomide et al Commun Biol. 2020 May 22;3(1):255. doi:

Insert

Integrase phiC31 coding sequence codon optimized for H. sapiens expression.

Expression

Mammalian

Available Since

May 6, 2020

Availability

Academic Institutions and Nonprofits only

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pECE HA AMPKa1 AS

Plasmid

#69831

Purpose

Expresses Expresses AMPK alpha 1 in mammalian cells

Depositor

Anne Brunet

Article

Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

Insert

5'-AMP-activated protein kinase catalytic subunit alpha-1 (PRKAA1 Human)

Tags

HA

Expression

Mammalian

Promoter

SV40 early promoter

Available Since

May 12, 2016

Availability

Academic Institutions and Nonprofits only

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pET-(pos36)GFP-9xGGS-Cre-6xHis

Plasmid

#62938

Purpose

Expression of (+36)GFP-GGS9-Cre-6xHis in bacterial cells

Depositor

David Liu

Article

Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

Insert

(ps36)GFP-9xGGS-Cre

Tags

(pos36)GFP and 6xHis tag

Expression

Bacterial

Promoter

T7

Available Since

May 18, 2015

Availability

Academic Institutions and Nonprofits only

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GEF-H1 FLARE191.pa

Plasmid

#134931

Purpose

GEF-H1 biosensor that has GEF activity.

Depositor

Klaus Hahn

Article

Azoitei et al J Cell Biol. 2019 Sep 2;218(9):3077-3097

Insert

GEF-H1(1-191)-yPet-4X linker-Cerulean3-GEF-H1(192-end)

Tags

None.

Expression

Bacterial and Mammalian

Promoter

CMV and T7

Available Since

Dec. 15, 2020

Availability

Academic Institutions and Nonprofits only

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pJFA345D4

Plasmid

#49238

Purpose

TET1-catalytic domain (CD) expression vector for TALE-TET1 fusion protein to induce targeted demethylation in mammalian cells

Depositor

Keith Joung

Article

Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038

Insert

TET1 tet methylcytosine dioxygenase 1 (TET1 Synthetic, Human)

Use

TALEN; Tale-tet1 expression vector

Promoter

EF1a

Available Since

Dec. 20, 2013

Availability

Academic Institutions and Nonprofits only

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pRiboI-APEX2m

Plasmid

#176843

Purpose

Expression of APEX2 from a codon-optimized gene (single-copy integrating plasmid)

Depositor

Jessica Seeliger

Article

Ahamed et al Methods Enzymol. 2022;664:267-289. doi:

Insert

pRibo-APEX2m

Use

Integrating

Expression

Bacterial

Promoter

pRibo, theophilline riboswitch inducible hsp60 pr…

Available Since

Jan. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pRibo-Sec-APEX2m

Plasmid

#176844

Purpose

Expression of APEX2 in the periplasm from a codon-optimized gene (multi-copy episomal plasmid)

Depositor

Jessica Seeliger

Article

Ahamed et al Methods Enzymol. 2022;664:267-289. doi:

Insert

pRibo-Sec-APEX2m

Use

Inducible

Expression

Bacterial

Promoter

pRibo, theophilline riboswitch inducible hsp60 pr…

Available Since

Jan. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPRv2 sgSp1

Plasmid

#174907

Purpose

Second generation sgRNA against Sp1

Depositor

Jane Clifford

Article

Swift et al Cell Rep. 2021 Mar 16;34(11):108840. doi

Insert

Specificity Protein 1 (SP1 Human)

Expression

Mammalian

Mutation

Missense mutation to mutate PAM sequence

Promoter

EFS-NS

Available Since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pIVT/Smarcc1

Plasmid

#122145

Purpose

For making Smarcc1 RNA

Depositor

Carmen Williams

Article

Miao et al Biol Reprod. 2018 Jan 9. pii: 4794936. d

Insert

Smarcc1 (Smarcc1 Mouse)

Use

For in vitro transcription

Promoter

T7

Available Since

March 20, 2019

Availability

Academic Institutions and Nonprofits only

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mCerulean3-EB3-C-20

Plasmid

#55417

Purpose

Localization: MT End Binding Protein, Excitation: 433, Emission: 475

Depositor

Michael Davidson

Article

Markwardt et al PLoS One. 2011 . 6(3):e17896.

Insert

EB3 (MAPRE3 Human)

Tags

mCerulean3

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 7, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA-Wnt7B-V5

Plasmid

#35934

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt7B (Wnt7b Human)

Tags

V5

Expression

Mammalian

Mutation

None

Promoter

CMV

Available Since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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Wnt4 STOP

Plasmid

#35873

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt4 (WNT4 Human)

Use

Gateway Cloning

Mutation

Silent Mutation C (492) --> T

Available Since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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Wnt7B STOP

Plasmid

#35878

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt7B (Wnt7b Human)

Use

Gateway Cloning

Mutation

None

Available Since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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Wnt10A STOP

Plasmid

#35883

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt10A (WNT10A Human)

Use

Gateway Cloning

Mutation

None

Available Since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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MLM3587

Plasmid

#43886

Purpose

Activation Domain: VP64; RVD of C-terminal 0.5 TALE repeat: NN (binds G)

Depositor

Keith Joung

Article

Maeder et al Nat Methods. 2013 Feb 10. doi: 10.1038/n

Insert

TALE-activator VP64 Backbone

Use

Tale transcriptional activator expression vector

Promoter

EF1alpha

Available Since

March 8, 2013

Availability

Academic Institutions and Nonprofits only

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