We narrowed to 58,883 results for: SAP

1. T10-T7-hMOV10

   Plasmid

   #185052

   Purpose

   expression of WT human MOV10

   Depositor

   Constance Ciaudo

   Article

   Arora et al EMBO Rep. 2022 Jul 20:e54458. doi: 10.15

   Insert

   cDNA WT hMOV10 (MOV10 Human)
   

   Use

   Tags

   T7

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMLS254

   Plasmid

   #73721

   Purpose

   SapTrap Halotag + Cbr-unc-119 donor plasmid

   Depositor

   Erik Jorgensen

   Article

   Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

   Insert

   Halotag + Cbr-unc-119
   

   Use

   CRISPR and Cre/Lox

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   March 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pMLS297

   Plasmid

   #73737

   Purpose

   SapTrap STOP codon + let-858 UTR N-terminal connector donor plasmid

   Depositor

   Erik Jorgensen

   Article

   Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

   Insert

   STOP codon + let-858 3'-UTR
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pMLS269

   Plasmid

   #73727

   Purpose

   SapTrap SL2::SV40-NLS C-terminal connector donor plasmid

   Depositor

   Erik Jorgensen

   Article

   Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

   Insert

   SL2 + SV40 NLS (gdp-2/gdp-3 intergenic region)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. USUV strain Vienna 2001 NS2B-NS3 protease fusion with non cleavable linker

   Plasmid

   #228658

   Purpose

   Bacterial expression of the fusion of the NS2B-NS3 protease from Usutu virus for biochemical assay

   Depositor

   ASAP Discovery, Frank von Delft

   Insert

   Usutu virus portion of NS2B protease fused to NS3
   

   Use

   Tags

   His-Twin Strep-Tev

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 18, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

6. SLEV strain Kern217 NS2B-NS3 protease fusion with non cleavable linker

   Plasmid

   #228657

   Purpose

   Bacterial expression of the fusion of the NS2B-NS3 protease from St. Louis encephalitis virus for biochemical assay

   Depositor

   ASAP Discovery, Frank von Delft

   Insert

   SLEV portion of NS2B protease fused to NS3
   

   Use

   Tags

   His-Twin Strep-Tev

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 18, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

7. ROCV strain SPH 34675 NS2B-NS3 protease fusion with non cleavable linker

   Plasmid

   #228655

   Purpose

   Bacterial expression of the fusion of the NS2B-NS3 protease from Rocio virus for biochemical assay

   Depositor

   ASAP Discovery, Frank von Delft

   Insert

   Rocio virus portion of NS2B protease fused to NS3
   

   Use

   Tags

   His-Twin Strep-Tev

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 18, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

8. POWV strain LB NS2B-NS3 protease fusion with non cleavable linker

   Plasmid

   #228654

   Purpose

   Bacterial expression of the fusion of the NS2B-NS3 protease from Powassan virus for biochemical assay

   Depositor

   ASAP Discovery, Frank von Delft

   Insert

   Powassan virus portion of NS2B protease fused to NS3
   

   Use

   Tags

   His-Twin Strep-Tev

   Expression

   Bacterial

   Mutation

   Alanine mutant to stop self-cleaving ..VEREEA*AL.…

   Promoter

   Available since

   Feb. 18, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

9. MVEV strain MVE-1-51 NS2B-NS3 protease fusion with non cleavable linker

   Plasmid

   #228653

   Purpose

   Bacterial expression of the fusion of the NS2B-NS3 protease from Murray Valley encephalitis virus for biochemical assay

   Depositor

   ASAP Discovery, Frank von Delft

   Insert

   MVEV portion of NS2B protease fused to NS3
   

   Use

   Tags

   His-Twin Strep-Tev

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 18, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

10. DENV-3 strain Phillippines/H87/1956 NS2B-NS3 protease fusion with non cleavable linker

    Plasmid

    #228651

    Purpose

    Bacterial expression of the fusion of the NS2B-NS3 protease from Dengue virus serotype 3 for biochemical assays

    Depositor

    ASAP Discovery, Frank von Delft

    Insert

    Dengue 3 portion of NS2B protease fused to NS3
    

    Use

    Tags

    His-Twin Strep-Tev

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 18, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

11. DENV-1 strain US/Hawaii/1944 NS2B-NS3 protease fusion with non cleavable linker

    Plasmid

    #228648

    Purpose

    Bacterial expression of the fusion of the NS2B-NS3 protease from Dengue virus serotype 1 for biochemical assay

    Depositor

    ASAP Discovery, Frank von Delft

    Insert

    Dengue 1 portion of NS2B protease fused to NS3
    

    Use

    Tags

    His-Twin Strep-Tev

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 18, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

12. SARS-CoV-2 isolate:Wuhan-Hu-1 Nucleocapsid Protein (C-term oligomerization domain)

    Plasmid

    #228641

    Purpose

    Bacterial expression of the SARS-CoV-2 N protein C terminal oligomerization domain used for crystallization assays.

    Depositor

    ASAP Discovery, Frank von Delft

    Insert

    SARS-CoV-2 N protein (C terminal domain) (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    His-Tev

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 18, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

13. pPB-cT3G-cERP2-PAX5

    Plasmid

    #222575

    Purpose

    PiggyBac transposon plasmid for doxycycline inducible expression of PAX5

    Depositor

    George Church

    Article

    Pierson Smela et al bioRxiv 2024.05.31.596483

    Insert

    PAX5 (PAX5 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pJRK108

    Plasmid

    #173732

    Purpose

    donor plasmid for SapTrap assembly

    Depositor

    Mike Boxem

    Article

    Kroll et al Genetics. 2021 Dec 10;219(4). pii: 63804

    Insert

    MP
    

    Use

    Other

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pMLS292

    Plasmid

    #73725

    Purpose

    SapTrap 2xNLS-mCherry + Cbr-unc-119 donor plasmid

    Depositor

    Erik Jorgensen

    Article

    Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

    Insert

    2xNLS-mCherry + Cbr-unc-119
    

    Use

    CRISPR and Cre/Lox

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    March 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. FUGW-APPwt-T2A-mCherry

    Plasmid

    #190803

    Purpose

    Express wild-type APP from Ubiquitin C promoter, co-expressing mCherry

    Depositor

    Ronald Hart

    Insert

    APP (APP Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Ubi C

    Available since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. HA-Clover-HIF-2alpha Wildtype

    Plasmid

    #163371

    Purpose

    Express HA- and Clover-tagged HIF-2alpha wildtype plasmid

    Depositor

    Violaine Sée

    Insert

    Hypoxia Inducible Factor 2 alpha (EPAS1 Human)
    

    Use

    Tags

    HA- and Clover-tagged

    Expression

    Mammalian

    Mutation

    Wildtype

    Promoter

    CMV Promoter

    Available since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCHAP2

    Plasmid

    #206847

    Purpose

    A plasmid containing a 15,249 bp fragment of the P. tricornutum chloroplast genome. The fragment is flanked by SapI recognition sites, allowing it to be released from the cloning vector pSAP1.

    Depositor

    Bogumil Karas

    Article

    Walker et al Plant Physiol. 2023 Dec 19:kiad670. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Jan. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCHAP1

    Plasmid

    #206846

    Purpose

    A plasmid containing a 14,519 bp fragment of the P. tricornutum chloroplast genome. The fragment is flanked by SapI recognition sites, allowing it to be released from the cloning vector pSAP1.

    Depositor

    Bogumil Karas

    Article

    Walker et al Plant Physiol. 2023 Dec 19:kiad670. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Jan. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pXF89

    Plasmid

    #139030

    Purpose

    MosSCI SapTrap assembly: Cassette 2 for 4- or 5-cassette system

    Depositor

    Erik Griffin

    Article

    Fan et al G3 (Bethesda). 2019 Dec 17. pii: g3.119.

    Insert

    halotag (no STOP codon, PATC introns)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge