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We narrowed to 2,617 results for: pCas

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Showing: 681 - 700 of 2617 results

  2. pUC CBA-SpCas9N863Anickase.EF1a-BFP.sgLMNApair1

    Plasmid
    #98976
    Purpose
    Cas9 Homologous Recombination Reporter. SpCas9N863A nickase and a pair of sgRNAs targeting hLMNA. Generates breaks with 44bp 3' overhangs. TagBFP.
    Depositor
    Jan Karlseder
    Publication
    Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    LMNA sgRNAs pair 1 and Cas9(N863A)
    Expression
    Mammalian
    Available since
    Nov. 29, 2017
    Availability
    Academic Institutions and Nonprofits only
    98976-plasmid-map-sequence-id-199220

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  3. pUC CBA-SpCas9D10Anickase.EF1a-BFP.sgLMNApair1

    Plasmid
    #98972
    Purpose
    Cas9 Homologous Recombination Reporter. SpCas9D10A nickase and a pair of sgRNA targeting hLMNA. Generates breaks with 44bp 5' overhangs. TagBFP.
    Depositor
    Jan Karlseder
    Publication
    Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    LMNA sgRNAs pair 1 and Cas9(D10A)
    Expression
    Mammalian
    Available since
    Nov. 29, 2017
    Availability
    Academic Institutions and Nonprofits only
    98972-plasmid-map-sequence-id-199211

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  4. pX330-Flag-eSpCas9-D10A

    Plasmid
    #80449
    Purpose
    enhanced SpCas9 mammalian expression vector
    Depositor
    Ervin Welker
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ehSpCas9_D10A
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Promoter
    CBh
    Available since
    Aug. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    80449-plasmid-map-sequence-id-157945

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  7. pCMV-T7-SpCas9-VRER-P2A-EGFP (RTW3160)

    Plasmid
    #139991
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized SpCas9-VRER(D1135V/G1218R/R1335E/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Publication
    Walton et al Science. 2020 Apr 17;368(6488):290-296.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human codon optimized SpCas9-VRER with BPNLS-3xFLAG-P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    VRER=D1135V/G1218R/R1335E/T1337R
    Promoter
    CMV and T7
    Available since
    March 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    139991-plasmid-map-sequence-id-275523

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  8. pX330-Flag-SpCas9-HF1 (without sgRNA)

    Plasmid
    #92102
    Purpose
    Expression plasmid for human codon-optimized high-fidelity SpCas9-HF1, px330-like backbone (without U6-sgRNA coding sequence)
    Depositor
    Ervin Welker
    Publication
    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    3xFlag-NLS-Streptococcus pyogenes Cas9-HF1-NLS
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    N497A, R661A, Q695A, Q926A
    Promoter
    Cbh
    Available since
    Oct. 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    92102-plasmid-map-sequence-id-197631

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  11. CROPseq-multi-v2-SpCas9-P2A-Puro-BsmBI_entry (pRW1512)

    Plasmid
    #225756
    Purpose
    Entry vector to clone sgRNA(s) into the CROPseq-multi-v2 construct with expression of SpCas9 and Puromycin resistance; v2 is compatible with T7 in vitro transcription detection
    Depositor
    Paul Blainey
    Publication
    Walton et al bioRxiv [Preprint]. 2024 Mar 17:2024.03.
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Available since
    Sept. 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    225756-plasmid-map-sequence-id-446064

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  14. pX330-Flag-eSpCas9 (without sgRNA)

    Plasmid
    #92354
    Purpose
    Expression plasmid for human codon-optimized high-fidelity eSpCas9 (without U6-sgRNA coding sequence)
    Depositor
    Ervin Welker
    Publication
    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    3xFLAG-NLS-Streptococcus pyogenes enhanced Cas9-NLS
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    K848A, K1003A, R1060A
    Promoter
    Cbh
    Available since
    Oct. 30, 2017
    Availability
    Academic Institutions and Nonprofits only
    92354-plasmid-map-sequence-id-197504

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  15. pCSD_2xNLS-SpCas9MT3-NLS-3xHA-NLS-dSaCas9-2xNLS

    Plasmid
    #107318
    Purpose
    Expresses SpCas9 (R1335K) fused to dSaCas9 in mammalian cells
    Depositor
    Scot Wolfe
    Publication
    Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SpCas9-SaCas9 fusion
    Use
    CRISPR
    Tags
    NLS
    Expression
    Mammalian
    Mutation
    SpCas9 (R1335K) fused to nuclease dead SaCas9 (D1…
    Promoter
    CMV IE94
    Available since
    Nov. 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    107318-plasmid-map-sequence-id-204674

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  16. pCSD_2xNLS-SpCas9WT-NLS-3xHA-NLS-SaCas9WT-2xNLS

    Plasmid
    #107320
    Purpose
    Expresses SpCas9 fused to SaCas9 in mammalian cells
    Depositor
    Scot Wolfe
    Publication
    Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SpCas9-SaCas9 fusion
    Use
    CRISPR
    Tags
    NLS
    Expression
    Mammalian
    Mutation
    SpCas9 is fused to SaCas9
    Promoter
    CMV IE94
    Available since
    Nov. 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    107320-plasmid-map-sequence-id-204686

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  18. pCMV-T7-ABE8.20m-nSpCas9-P2A-EGFP (KAC1158)

    Plasmid
    #185915
    Purpose
    pCMV and pT7 plasmid encoding human codon optimized ABE8.20m A-to-G base editor with nickase SpCas9(D10A) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Publication
    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human codon optimized ABE8.20m-nSpCas9-P2A-EGFP
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS and BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    nSpCas9=D10A
    Promoter
    CMV and T7
    Available since
    July 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    185915-plasmid-map-sequence-id-367353

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Showing: 681 - 700 of 2617 results