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Plasmid#155181PurposeExpresses colicin E1 residues 1-364DepositorInsertcolicin E1 residues 1-364
UseTagsC-terminal 6x Histidine tagExpressionBacterialMutationtruncation of colicin E1 including 1-364PromoterT7Available sinceAug. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-mBoNT/F
Plasmid#168015PurposeExpresses MBP-BoNT/F-LC(1-428)-6xHis in bacterial cellsDepositorInsertMBP-BoNT/F-LC(1-428)-6xHis
UseTagsHisx6 and maltose binding proteinExpressionBacterialMutationPromoterAvailable sinceApril 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET32-sLatherin
Plasmid#61685Purposebacterial expression of the horse sweat/saliva protein Latherin as a His-tagged thioredoxin fusionDepositorInsertLatherin (LATH horse)
UseTagsHIS and Trx-Tag thioredoxin proteinExpressionBacterialMutationCodon optimized for E.coliPromoterT7Available sinceMarch 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pETDuet-TFIIF
Plasmid#171082PurposeCo-expresses human TFIIF. The resulting plasmid was used to generate a expression construct encoding 2 subunits, with His-tag on RAP30. Originally from MN Gonzalez, was submitted with permissionDepositorUseTagsHisExpressionBacterialMutationPromoterT7 promoterAvailable sinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
Pet22b-YAP
Plasmid#166443PurposeBacterial expression of 6x His tagged YAPDepositorInsertYAP (YAP1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET-BoNT/X
Plasmid#168017PurposeExpresses BoNT/X-LC(1-439)-6xHis in bacterial cellsDepositorInsertBoNT/X-LC(1-439)-6xHis
UseTagsHisx6ExpressionBacterialMutationPromoterAvailable sinceApril 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28b-NM23-H1
Plasmid#157987PurposeExpresses NM23-H1 in E.coli cellDepositorInsertNM23-H1 (NME1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET-E(4130)A2
Plasmid#168021PurposeExpresses E(4130)A2-6xHis in bacterial cellsDepositorInsertE(4130)A2-6xHis
UseTagsHisx6ExpressionBacterialMutationPromoterAvailable sinceApril 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDB179-ThermoPETase
Plasmid#218698PurposeEncodes codon optimized ThermoPETase enzyme for bacterial expressionDepositorInsertThermoPETase
UseTags10xHisExpressionBacterialMutationPromoterAvailable sinceMay 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDB179-BhrPETase
Plasmid#218692PurposeEncodes codon optimized BhrPETase enzyme for bacterial expressionDepositorInsertBhrPETase
UseTags10xHisExpressionBacterialMutationPromoterAvailable sinceMay 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEThT-SAHS1
Plasmid#90025PurposeTo express His-tagged SAHS1 in bacteriaDepositorInsertSAHS1
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceAug. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET23a-HsRPS27a
Plasmid#69561PurposeBacterial expression; in vitro transcription/translation (T7)DepositorInsertRPS27A (RPS27A Human)
UseTagsExpressionBacterialMutationPromoterT7Available sinceDec. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pETM6-G6-vioABECD
Plasmid#66536PurposepETM6 vector containing vioA-vioB-vioE-vioC-vioD in monocistronic configuration with G6 promoterDepositorInsertsVioA
VioB
VioE
VioC
VioD
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterMutant T7 Promoter - G6Available sinceJuly 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET28a-RILP
Plasmid#74600Purposeused for expression in E. coli and purificationDepositorInsertRab-interacting lysosomal protein (RILP Human)
UseTagsFLAG, StrepII, and superfolder GFPExpressionBacterialMutationPromoterT7Available sinceJune 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET30b-7Y-SrtA
Plasmid#105601Purposeexpresses 7Y sortase A variant in E.coliDepositorInsertS.aureus Sortase A, 7Y mutant
UseTagsHis tagExpressionBacterialMutationPromoterT7Available sinceJan. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a(+)/FaGH16A
Plasmid#100910PurposeExpression of the Beta-1,3-1,4-Endo-Laminarinase FaGH16A in E.coliDepositorInsertFaGH16A
UseTagsHis-TagExpressionBacterialMutationPromoterAvailable sinceSept. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET30-2-GPD1
Plasmid#83911Purposestable overexpressionDepositorInsertglycerol-3-phosphate dehydrogenase 1 (GPD1 Human)
UseTags6x His tagsExpressionBacterialMutationPromoterT7Available sinceOct. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET51b_SNAP-ecDHFR
Plasmid#187108PurposeExpression of SNAP-ecDHFR fusion in bacteriaDepositorInsertSNAP-ecDHFR
UseTagsHisx10 and StrepExpressionBacterialMutationPromoterT7Available sinceJuly 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET19b+H2B
Plasmid#157809PurposeExpression of human histone H2BDepositorInsertH2B
UseTagsExpressionBacterialMutationPromoterT7Available sinceSept. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET32a-DEST
Plasmid#84652PurposeE. coli expression vector derived from pET32 with Gateway cassette.DepositorTypeEmpty backboneUseTagsHis, S tag, and Trx TagExpressionBacterialMutationPromoterT7Available sinceNov. 7, 2016AvailabilityAcademic Institutions and Nonprofits only