We narrowed to 1,071 results for: proc.2
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Plasmid#67833PurposeExpression of FL-EKLF driven by SV40 promoterDepositorInsertEKLF (Klf1 Mouse)
TagsFLAGExpressionMammalianMutationFull-length mEKLF is aa 20-376; amino acid 19 is …PromoterSV40Available SinceAug. 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGEX4T2 gp78 G2BR mt / JM26
Plasmid#13308DepositorInsertgp78 (AMFR Human)
TagsGSTExpressionBacterialMutationcDNA corresponds to aa309-643 derived from human …Available SinceOct. 16, 2006AvailabilityAcademic Institutions and Nonprofits only -
pSN672
Plasmid#177362PurposeExpresses human KIF1A(1-393) fused with leucine zipper and His tagDepositorAvailable SinceAug. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
p6595 MSCV-IP-N-HA-GPRC5B
Plasmid#34894DepositorAvailable SinceFeb. 8, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCI-Neo-gp78 Cue-m1,2 / JM22
Plasmid#13305DepositorInsertgp78 (AMFR Human)
ExpressionMammalianMutationM467G ; F468G ; P469R ; V476R ; D479V ; L480DAvailable SinceOct. 16, 2006AvailabilityAcademic Institutions and Nonprofits only -
pGEX4T2 gp78 CD-m1,2 / JM25
Plasmid#13307DepositorInsertgp78 (AMFR Human)
TagsGSTExpressionBacterialMutationcDNA corresponds to aa309-643 derived from human …Available SinceOct. 16, 2006AvailabilityAcademic Institutions and Nonprofits only -
pCI-Neo-gp78 G2BR mt / JM23
Plasmid#13306DepositorInsertgp78 (AMFR Human)
ExpressionMammalianMutationQ579A ; R580A ; M581A ; L582G ; V583G ; Q584GAvailable SinceOct. 16, 2006AvailabilityAcademic Institutions and Nonprofits only -
pPU-huMDA5-miR30-L1221
Plasmid#174223PurposeAll-in-one huMDA5 rescue-control shRNA knockdown vectorDepositorAvailable SinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pscALPSpuro-MDA5-S88E
Plasmid#174227PurposeExpresses human MDA5 S88E mutantDepositorAvailable SinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
cacna2d1 R241A pMT2
Plasmid#206110Purposeexpression of rat alpha2delta-1 calcium channel auxillary subunit and with R421A mutation that disrupts Gabapentin bindingDepositorAvailable SinceNov. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
cacna2d1 R241A HA pMT2
Plasmid#206111Purposeexpression of rat alpha2delta-1 calcium channel auxillary subunit with a single HA tag inserted after aa549 and with an R421A mutation that disrupts Gabapentin bindingDepositorAvailable SinceNov. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
Cav2.2 BBS W391A pcDNA3
Plasmid#206077PurposeCav2.2 calcium channel with an exofacial double Bungarotoxin Binding Site motif in domain II and a mutation (W391A) in the ?1-interacting domain (AID) in the I-II loop that prevents Beta bindingDepositorAvailable SinceSept. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSN554
Plasmid#177361PurposeExpresses human KIF1A(1-393) fused with leucine zipper, mScarlet and StrepII tagDepositorInsertKIF1A (KIF1A Human)
TagsLeucine zipper, StrepII tag, and mScarletExpressionBacterialPromoterT7Available SinceAug. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSN687
Plasmid#177363PurposeExpresses human KIF1A(1-393)(P305L) fused with leucine zipper and His tagDepositorInsertKIF1A (KIF1A Human)
TagsHis tag and Leucine zipperExpressionBacterialMutationchanged Proline 305 to LeucinePromoterT7Available SinceAug. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCJ200
Plasmid#162673PurposepET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating S136C and a sequence Tyr75:Phe104 to introduce a 6th disulfide bond as in AoFaeB-2.DepositorInsertMHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1)
TagsHisExpressionBacterialMutationS136C and a sequence Y75-F104 to introduce a 6th…PromoterT7/lacAvailable SinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCJ210
Plasmid#162683PurposepET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating S136C, G489C, S530C, and a sequence Tyr75:Phe104 to introduce a 6th and 7th disulfide bond as in AoFaeB-2.DepositorInsertMHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1)
TagsHisExpressionBacterialMutationS136C, G489C, S530C, and a sequence Y75-F104 to i…PromoterT7/lacAvailable SinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNB_EA_3EGFP
Plasmid#185967PurposeExpresses EGFP from PR promoter in E.coli Dh5alpha cellDepositorInsertPr-Egfp cassette in forward direction in Network Brick
UseSynthetic BiologyPromoterPRAvailable SinceAug. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-myc-S (Wuhan variant)
Plasmid#169846PurposeMammalian expression plasmid for S of SARS-CoV-2 (Wuhan variant) with N-terminal c-myc tagDepositorInsertProtein S
TagsHA leader and c-myc tagExpressionMammalianMutationCodon-optimized for human cell expression.PromoterCMVAvailable SinceJan. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNB_EA_R1CiM_4EGFP_R3EGFPi2
Plasmid#185968PurposeMolecular IMPLY gate, encodes mutant Ci protein from Plteto-1 promoter, and EGFP form PR and PlTataa promoter. Must be expressed in E.coli DH5alphaz1 for experimental characterization.DepositorInsertMolecular IMPLY gate
UseSynthetic BiologyAvailable SinceOct. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNB_EA_R3tdTomatoi2
Plasmid#185969PurposeExpresses tdTomato from PR promoter with optimized RBS (i2) in E.coli Dh5alpha cell. The construct is in reverse direction network brick.DepositorInsertPr-i2rbs-tdTomato cassette in reverse direction in Network Brick
UseSynthetic BiologyPromoterPRAvailable SinceAug. 29, 2022AvailabilityAcademic Institutions and Nonprofits only