We narrowed to 7,139 results for: 11

1. Flag‐mTim3 (del.Cyto)

   Plasmid

   #49209

   Purpose

   expresses Flag tagged mouse Tim3 fragment (no cytosolic domain)

   Depositor

   Lawrence Kane

   Article

   Lee et al Mol Cell Biol. 2011 Oct;31(19):3963-74.

   Insert

   Tim3 (del.Cyto) (Havcr2 Mouse)
   

   Use

   Tags

   CD8 signal sequence and FLAG

   Expression

   Mammalian

   Mutation

   cytosolic domain deleted, no Tim3 signal sequence…

   Promoter

   Ef1a

   Available since

   Dec. 17, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. MSCV-EVA1ext-hFcIgG3-IRES-YFP

   Plasmid

   #64926

   Purpose

   To express extracellular portion of EVA1 (mouse) fused with human Fc(IgG3)

   Depositor

   Pau Serra

   Article

   Garabatos et al Monoclon Antib Immunodiagn Immunother. 2

   Insert

   mouse EVA1 fused with human FcIgG3 (Mpzl2, IGHG3 Human, Mouse)
   

   Use

   Retroviral

   Tags

   IRES-YFP

   Expression

   Mammalian

   Mutation

   Promoter

   LTR

   Available since

   June 5, 2015

   Availability

   Academic Institutions and Nonprofits only

3. Flag‐mTim3 (T2 truncation)

   Plasmid

   #49211

   Purpose

   expresses Flag tagged mouse Tim3 (T2 truncation)

   Depositor

   Lawrence Kane

   Article

   Lee et al Mol Cell Biol. 2011 Oct;31(19):3963-74.

   Insert

   Tim3 T2 truncation (Havcr2 Mouse)
   

   Use

   Tags

   FLAG and signal sequence

   Expression

   Mammalian

   Mutation

   T2 truncation ( lacks all but one tyrosine, which…

   Promoter

   EF1a

   Available since

   Dec. 11, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. Flag‐mTim3 (T1 truncation)

   Plasmid

   #49210

   Purpose

   expresses Flag tagged mouse Tim3 (T1 truncation)

   Depositor

   Lawrence Kane

   Article

   Lee et al Mol Cell Biol. 2011 Oct;31(19):3963-74.

   Insert

   Tim3 T1 truncation (Havcr2 Mouse)
   

   Use

   Tags

   FLAG and signal sequence

   Expression

   Mammalian

   Mutation

   T1 truncation ( contains all but the three most C…

   Promoter

   EF1a

   Available since

   Dec. 11, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. API5_pLX307

   Plasmid

   #98315

   Purpose

   Lentiviral expression of API5

   Depositor

   Sefi Rosenbluh, William Hahn

   Article

   Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

   Insert

   API5 (API5 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   E1Fa

   Available since

   Aug. 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1-nAChr-Alpha6-YFP

   Plasmid

   #50483

   Purpose

   this report is the first to directly measure nAChr subunit stoichiometry using FRET and plasma membrane localization of Alpha6 and Beta3 containing receptors using TIRF

   Depositor

   Henry Lester

   Article

   Drenan et al Mol Pharmacol. 2008 Jan;73(1):27-41. Epu

   Insert

   nAChr-alpha6 (Chrna6 Mouse)
   

   Use

   Tags

   YFP fusion in M3-M4 loop (after residue A405)

   Expression

   Mammalian

   Mutation

   Gly-Ala-Gly flexible linker flanking the YFP open…

   Promoter

   CMV

   Available since

   Jan. 13, 2014

   Availability

   Academic Institutions and Nonprofits only

7. pGEX4T-1 Slp2-a E11A/R32A

   Plasmid

   #40063

   Depositor

   Fernando Martin-Belmonte

   Article

   Galvez-Santisteban et al Nat Cell Biol. 2012 Jul 22. doi: 10.1038

   Insert

   Slp2-a E11A/R32A (Sytl2 Mouse)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Glutamate 11 to Alanine, Arginine 32 to Alanine

   Promoter

   Tac

   Available since

   Nov. 9, 2012

   Availability

   Academic Institutions and Nonprofits only

8. pEGFP-C1 Slp2-a E11A/R32A

   Plasmid

   #40055

   Depositor

   Fernando Martin-Belmonte

   Article

   Galvez-Santisteban et al Nat Cell Biol. 2012 Jul 22. doi: 10.1038

   Insert

   Slp2-a E11A/R32A (Sytl2 Mouse)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Glutamate 11 to Alanine, Arginine 32 to Alanine

   Promoter

   CMV promoter

   Available since

   Nov. 9, 2012

   Availability

   Academic Institutions and Nonprofits only

9. pRP.USH2A

   Plasmid

   #226555

   Purpose

   Minigene encoding USH2A exon 13 and surrounding introns/exons

   Depositor

   Dave Wee

   Article

   Tabaglio et al Mol Ther Nucleic Acids. 2025 Jan 7;36(1)

   Insert

   USH2A (USH2A Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLentiCRISPR v2 puro mGSDME gRNA2

    Plasmid

    #223522

    Purpose

    Knocking out mGSDME in mouse cells

    Depositor

    Judy Lieberman

    Article

    Zhang et al Nature. 2020 Mar;579(7799):415-420. doi:

    Insert

    GSDME (GSDME Mouse)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pLentiCRISPR v2 puro mGSDME gRNA1

    Plasmid

    #223521

    Purpose

    Knocking out mGSDME in mouse cells

    Depositor

    Judy Lieberman

    Article

    Zhang et al Nature. 2020 Mar;579(7799):415-420. doi:

    Insert

    GSDME (GSDME Mouse)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCIneo-lambdaN-HA-HsEDC4-del974-1265_V

    Plasmid

    #147786

    Purpose

    Mammalian Expression of HsEDC4-del974-1265

    Depositor

    Elisa Izaurralde

    Article

    Braun et al Nat Struct Mol Biol. 2012 Dec;19(12):132

    Insert

    HsEDC4-del974-1265 (EDC4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    one non silent mutation I286T compared to the seq…

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pD649-HAsp-PSG11-COMP5AP-AviTag-9xHis

    Plasmid

    #157304

    Purpose

    Mammalian expression of secreted protein fused to COMP5AP-AviTag-9xHis.

    Depositor

    Chris Garcia

    Article

    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

    Insert

    PSG11 (PSG11 Human)
    

    Use

    Tags

    COMP5AP-AviTag-9xHis

    Expression

    Mammalian

    Mutation

    Promoter

    CMV/SP6

    Available since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pD649-HAsp-PSG9-COMP5AP-AviTag-9xHis

    Plasmid

    #157388

    Purpose

    Mammalian expression of secreted protein fused to COMP5AP-AviTag-9xHis.

    Depositor

    Chris Garcia

    Article

    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

    Insert

    PSG9 (PSG9 Human)
    

    Use

    Tags

    COMP5AP-AviTag-9xHis

    Expression

    Mammalian

    Mutation

    Promoter

    CMV/SP6

    Available since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. W118-1_eGFP

    Plasmid

    #180379

    Purpose

    lentiviral transduction of eGFP gene

    Depositor

    Huda Zoghbi

    Article

    Lee et al EMBO J. 2021 Apr 1;40(7):e106106. doi: 1

    Insert

    eGFP (eGFP )
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. His-MBP-IST1 N-terminal domain

    Plasmid

    #184805

    Purpose

    Bacterial expression of IST1 N-terminal domain

    Depositor

    James Hurley

    Article

    Cada et al Proc Natl Acad Sci U S A. 2022 Jul 19;11

    Insert

    IST1 (IST1 Human)
    

    Use

    Tags

    His6-MBP

    Expression

    Bacterial

    Mutation

    aa 1-187 only

    Promoter

    T7

    Available since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAAV-hSyn-GFP-IRES-hSNCA (A11P/V70P)

    Plasmid

    #185717

    Purpose

    AAV expression of GFP and human α-Synuclein with A11P and V70P mutations from hSyn promoter

    Depositor

    Jun Ding

    Article

    Albarran et al Nat Neurosci. 2023 Jun;26(6):997-1007. d

    Insert

    synuclein, alpha (SNCA Human)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Changed Ala 11 to Pro, Val 70 to Pro

    Promoter

    hSyn

    Available since

    June 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. His-MBP-CHMP1B, S2C

    Plasmid

    #184803

    Purpose

    Bacterial expression of CHMP1B (S2C mutation)

    Depositor

    James Hurley

    Article

    Cada et al Proc Natl Acad Sci U S A. 2022 Jul 19;11

    Insert

    CHMP1B (CHMP1B Human)
    

    Use

    Tags

    His6-MBP

    Expression

    Bacterial

    Mutation

    S2C

    Promoter

    T7

    Available since

    June 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pGEX-2T-CBP(1603-2678)

    Plasmid

    #183774

    Purpose

    Express GST-CBP(1603-2678) in E. coli. Purified GST-CBP(1603-2678) was used for in vitro acetylation assays.

    Depositor

    Peter Harte

    Article

    Tie et al Mol Cell Biol. 2012 Jun;32(12):2323-34.

    Insert

    nej (nej Fly)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    April 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pSUMO GLTP

    Plasmid

    #170732

    Purpose

    The plasmids contain inserts (ORFs) for expressing various members of the GlycoLipid Transfer Protein (GLTP) superfamily.

    Depositor

    Rhoderick Brown

    Article

    Ochoa-Lizarralde et al J Biol Chem. 2018 Oct 26;293(43):16709-1

    Insert

    GLTP (GLTP Human)
    

    Use

    Tags

    SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge