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Showing: 6981 - 7000 of 8645 results

  4. HSC1-GiP

    Plasmid
    #58254
    Purpose
    This self-inactivated retroviral vector has an internal human EF1alpha promoter driving the expression of a bicistronic EGFP ires Puromycin cassette
    Depositor
    James Ellis
    Article
    Rival-Gervier et al Mol Ther. 2013 Aug;21(8):1536-50. doi: 1
    Insert
    Enhanced Green Fluorescence Protein, Puromycin resistance gene
    Use
    Retroviral
    Expression
    Mammalian
    Promoter
    Human EF1-alpha
    Available Since
    Oct. 10, 2014
    Availability
    Academic Institutions and Nonprofits only
    58254-plasmid-map-sequence-id-321491

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  6. pLaNGo11-7

    Plasmid
    #126055
    Purpose
    A self-assembling split-GFP based Golden Gate vector to determine protein targeting to plastids in plant cell
    Depositor
    Ralf-Bernd Klösgen
    Article
    Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs
    Inserts
    chloroplastic FNR transit peptide
    ccdB cassette (BsaI)
    GFP11x7 (5 aa linker)
    Use
    Binary
    Tags
    GFP1-10
    Expression
    Plant
    Available Since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    126055-plasmid-map-sequence-id-247152

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  7. BPK2101

    Plasmid
    #65770
    Purpose
    Bacterial expression plasmid for S. aureus Cas9 & sgRNA (need to clone in spacer into BsaI sites): T7-humanSaCas9-NLS-3xFLAG-T7-BsaIcassette-Sa-sgRNA
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature
    Insert
    mammalian codon-optimized SaCas9, and SaCas9 gRNA
    Use
    CRISPR
    Tags
    NLS-3xFlag
    Expression
    Bacterial
    Promoter
    T7 (x2)
    Available Since
    June 24, 2015
    Availability
    Academic Institutions and Nonprofits only
    65770-plasmid-map-sequence-id-112650

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  14. pOCC305

    Plasmid
    #118898
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for rec. protein with N-terminal TwinStrep-MBP tag, cleavable with 3C and C-terminal HIS6, cleavable with 3C
    Depositor
    Marc Bickle, PEPC MPI CBG
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-TwinStrep-MBP-3C-NotI-ccdB-AscI-3C-HIS6-stop-HindIII cassette
    Tags
    HIS6, cleavable with 3C protease and TwinStrep-MB…
    Expression
    Insect
    Promoter
    polH
    Available Since
    Jan. 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    118898-plasmid-map-sequence-id-232364

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  19. pAGK61

    Plasmid
    #228121
    Purpose
    Express node 1467 retron with split retron ncRNA and gRNA to edit (10bp barcode) EMX1 gene in human cells
    Depositor
    Seth Shipman
    Article
    Khan et al Nat Biotechnol. 2024 Sep 17. doi: 10.103
    Insert
    Retron node 1467, split retron ncRNA and gRNA to edit EMX1 gene (10bp barcode) in human cells
    Expression
    Mammalian
    Promoter
    CAG for RT, U6/H1 for ncRNA/gRNA
    Available Since
    Dec. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    228121-plasmid-map-sequence-id-455410

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Showing: 6981 - 7000 of 8645 results