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Plasmid#131326PurposegRNA to generate F97A cage-mutant EEDDepositorInsertEED-KO-gRNA-2
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceMarch 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
EED-KO-gRNA-1
Plasmid#131321PurposegRNA to knockout EED. Use with EED-KO-gRNA-2DepositorInsertEED-KO-gRNA-1
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceMarch 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCR8_TaNAM-A1
Plasmid#124574PurposeTaNAM-A1 CDS in the Gateway-compatible pCR8 vector.DepositorInsertTaNAM-A1
UseTopo gateway cloning vectorTagsExpressionMutationPromoterNoneAvailable sinceAug. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMH-HT_ygiF-TTM
Plasmid#127794PurposeExpresses the TTM/CYTH domain of ygiF in E. coli cellsDepositorInsertygiF (ygiF Escherichia coli)
UseTags6xHis tag + TEV cleavage siteExpressionBacterialMutationN-terminal TTM domain of ygiF (residues 1-200)PromoterAvailable sinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pNIC28-BSA4-S15
Plasmid#127821PurposeProduction of recombinant human ribosomal protein S15DepositorInsertRPS15 (RPS15 Human)
UseTagsExpressionBacterialMutationPromoterT7Available sinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pNH-TrxT-G-T136D-S15
Plasmid#127824PurposeProduction of recombinant human ribosomal protein S15DepositorInsertRPS15 (RPS15 Human)
UseTagsHis6 - Thioredoxin - TEVExpressionBacterialMutationT136DPromoterT7Available sinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pNH-TrxT-G-S10
Plasmid#127829PurposeProduction of recombinant human ribosomal protein S10DepositorInsertRPS10 (RPS10 Human)
UseTagsHis6 - Thioredoxin - TEVExpressionBacterialMutationPromoterT7Available sinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJL1-neoR
Plasmid#117048PurposeIn vitro expression of neoR (kanamycin resistance gene) from the T7 promoterDepositorInsertneoR
UseTagsExpressionBacterialMutationPromoterT7Available sinceMay 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pClodANL-FGF4
Plasmid#107874PurposeBacterial expression of FGF4-NanoLucDepositorInsertFGF4 (FGF4 Synthetic)
UseLuciferaseTagsNanoLucExpressionBacterialMutationPromoterpro DAvailable sinceApril 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAL832
Plasmid#119305Purposeprecusor outer membrane protein A, ompA, truncated after amino acid 176DepositorInsertprecursor outer membrane protein A, ompA, truncated after amino acid 176 including with mutations T95C and I153C
UseTagsnoneExpressionBacterialMutationtruncated after amino acid 176, mutations Threoni…PromoterlacAvailable sinceJan. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAL800
Plasmid#119306Purposeprecusor outer membrane protein A, ompA, with amino acids 1 through 171 deleted and mutations G244C and C290SDepositorInsertprecusor outer membrane protein A, ompA, with amino acids 1 through 171 deleted and mutations G244C and C290S
UseTagsnoneExpressionBacterialMutationamino acids 1 through 171 deleted, and mutatioms …PromoterlacAvailable sinceJan. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAL928
Plasmid#119853Purposeprecusor alkaline phosphatase, phoA, with C169S, C179S, C287S, C337S, A369C and A427CDepositorInsertprecursor alkaline phosphatase, phoA, with mutations C169S, C179S, C287S, C337S, A369C and A427C
UseTagsnoneExpressionBacterialMutationCysteine 169 to Serine, Cysteine 179 to Serine, C…PromoterT7Available sinceDec. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pVITRO-Fab_Theo-LUCID
Plasmid#119048PurposePlasmid encoding the mammalian expression of the Theophiline LUCID based on antibodies (BRET semi-synthetic biosensor)DepositorInsertsFab_Theo-LUCID_part1
Fab_Theo-LUCID_part2
UseTagsFLAG-tag, His-tag, and Strep-tagExpressionMammalianMutationPromoterEF-1α + CMV/SV40 enhancers and IRESAvailable sinceDec. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pVITRO-Fab_Quin-LUCID
Plasmid#119049PurposePlasmid encoding the mammalian expression of the Quinine LUCID based on antibodies (BRET semi-synthetic biosensor)DepositorInsertsFab_Quin-LUCID_part1
Fab_Quin-LUCID_part2
UseTagsFLAG-tag, His-tag, and Strep-tagExpressionMammalianMutationPromoterEF-1α + CMV/SV40 enhancers and IRESAvailable sinceDec. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pVITRO-Fab_MTX-LUCID
Plasmid#119047PurposePlasmid encoding the mammalian expression of the Metotraxate LUCID based on antibodies (BRET semi-synthetic biosensor)DepositorInsertsFab_MTX-LUCID_part1
Fab_MTX-LUCID_part2
UseTagsFLAG-tag, His-tag, and Strep-tagExpressionMammalianMutationPromoterEF-1α + CMV/SV40 enhancers and IRESAvailable sinceDec. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBAD-MTX-u36_Snifit
Plasmid#119045PurposePlasmid encoding the bacterial expression of the Snifit-MTX with unnatural amino-acid at the position 36 (FRET semi-synthetic biosensor)DepositorInsertMTX-u36_Snifit
UseTagsHis-tag and Strep-tagExpressionBacterialMutation36TAG R44L H45QPromoterpBADAvailable sinceNov. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBAD-MTX-u51_Snifit
Plasmid#119046PurposePlasmid encoding the bacterial expression of the Snifit-MTX with unnatural amino-acid at the position 51 (FRET semi-synthetic biosensor)DepositorInsertMTX-u51_Snifit
UseTagsHis-tag and Strep-tagExpressionBacterialMutation51TAG R44L H45QPromoterpBADAvailable sinceNov. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBAD-MTX-u23_Snifit
Plasmid#119044PurposePlasmid encoding the bacterial expression of the Snifit-MTX with unnatural amino-acid at the position 23 (FRET semi-synthetic biosensor)DepositorInsertMTX-u23_Snifit
UseTagsHis-tag and Strep-tagExpressionBacterialMutation23TAG R44L H45QPromoterpBADAvailable sinceNov. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCarCBADE
Plasmid#117757PurposeCodon optimized CarCBADE enzymes from Pectobacterium carotovorum in pBbaA5k backboneDepositorInsertCodon optimised CarCBA, Protein CarD, Ferredoxin CarE
UseTagsExpressionBacterialMutationPromoterPLac(UV5)Available sinceNov. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV7.1-Flag-NLS-hSPDEF delta2
Plasmid#100871PurposeMammalian expression of human SPDEF truncationDepositorInsertSPDEF (SPDEF Human)
UseTags3xFLAGExpressionMammalianMutationtruncated protein 71-246aaPromoterCMVAvailable sinceOct. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pINDUCER20-Flag-NLS-hSPDEF delta3
Plasmid#100876PurposeLentiviral expression of human SPDEF truncationDepositorInsertSPDEF (SPDEF Human)
UseLentiviralTags3xFLAGExpressionMammalianMutationtruncated protein 243-335aaPromoterTRE2Available sinceSept. 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV7.1-Flag-NLS-hSPDEF delta3
Plasmid#100872PurposeMammalian expression of human SPDEF truncationDepositorInsertSPDEF (SPDEF Human)
UseTags3xFLAGExpressionMammalianMutationtruncated protein 244-335aaPromoterCMVAvailable sinceSept. 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV7.1-Flag-NLS-hSPDEF delta1
Plasmid#100870PurposeMammalian expression of human SPDEF truncationDepositorInsertSPDEF (SPDEF Human)
UseTags3xFLAGExpressionMammalianMutationtruncated protein 1-248aaPromoterCMVAvailable sinceSept. 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pWN10042
Plasmid#89052PurposeGF-ori-FP plasmid for GFxFP assayDepositorInsertsEGFP part 1
EGFP part 2
UseCRISPRTagsExpressionMammalianMutationPromoterCAG and noneAvailable sinceApril 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBRC169-DXMT
Plasmid#79822PurposeDsREDmonomer-based BiFC vector for plants (Positive control: DXMT)DepositorInsertCaDXMT1
UseReporter plasmidTagsRC169/DsREDmonomer (169–225)ExpressionPlantMutationPromoterCaMV 35S promoter and CaMV35SAvailable sinceDec. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBRN168-DXMT
Plasmid#79821PurposeDsREDmonomer-based BiFC vector for plants (Positive control: DXMT)DepositorInsertCaDXMT1
UseReporter plasmidTagsRN168/DsREDmonomer (1–168)ExpressionPlantMutationPromoterCaMV 35S promoter and CaMV35SAvailable sinceDec. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBRC169-MXMT
Plasmid#79820PurposeDsREDmonomer-based BiFC vector for plants (Positive control: MXMT)DepositorInsertCaMXMT1
UseReporter plasmidTagsRC169/DsREDmonomer (169–225)ExpressionPlantMutationPromoterCaMV 35S promoter and CaMV35SAvailable sinceDec. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTCW018
Plasmid#83531PurposeAromatic amino acid inducible expression of alpha pheromone, and pheromone inducible exression of an ARO7 RNA interference hairpinDepositorInsertpARO9-mf_2-pFUS1J2-ARO7i-CYC1t
UseTagsExpressionYeastMutationPromoterpARO9/pFUS1J2Available sinceOct. 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pXY029 (mEos2-MTSBs)
Plasmid#72652PurposeInducible expression of mEos2-MTSBs in bacteriaDepositorInsertmEos2-MTSBs
UseTagsmEos2ExpressionBacterialMutationPromoterT5-lacAvailable sinceFeb. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pONL-C1_RhoB
Plasmid#65698PurposeExpresses ONL-RhoB in mammalian cellsDepositorInsertRhoB (RHOB Human)
UseLuciferaseTagsONLExpressionMammalianMutationPromoterCMVAvailable sinceJan. 19, 2016AvailabilityAcademic Institutions and Nonprofits only