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We narrowed to 11,809 results for: emb

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Showing: 6981 - 7000 of 11809 results

  1. pD10AH840AhCas9 (GB1041)

    Plasmid
    #68207
    Purpose
    Provides the human codon optimized CDS of Cas9 protein with mutated (D10A, H840A) and inactivated catalytic domains as a level 0 GoldenBraid part
    Depositor
    Diego Orzaez
    Publication
    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.
    Insert
    Cas9 coding region with mutated (D10A, H840A) and inactivated catalytic domains (human codon optimised)
    Use
    CRISPR and Synthetic Biology
    Mutation
    BsaI and BsmBI sites removed; human codon optimis…
    Available since
    March 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    68207-plasmid-map-sequence-id-135575

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  3. pCMV-myc-mIFITM3-Y20A

    Plasmid
    #58460
    Purpose
    Expresses murine IFITM3-Y20A with an N-terminal myc tag in mammalian cells.
    Depositor
    Jacob Yount
    Publication
    Chesarino et al J Biol Chem. 2014 Apr 25;289(17):11986-9
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    IFITM3 (Ifitm3 Mouse)
    Tags
    myc
    Expression
    Mammalian
    Mutation
    Tyrosine 20 to alanine
    Promoter
    CMV IE
    Available since
    Sept. 10, 2014
    Availability
    Academic Institutions and Nonprofits only

  5. pCVL.SSA TLR (Ani)

    Plasmid
    #45577
    Purpose
    Codes for the SSA-TLR with the target site for I-Ani I in a lentiviral backbone
    Depositor
    Andrew Scharenberg
    Publication
    Kuhar et al Nucleic Acids Res. 2013 Oct 10.
    Inserts
    5' iRFP arm
    eGFP with I-Ani I TS
    +3 mCherry
    3' iRFP arm
    Use
    Lentiviral
    Expression
    Bacterial and Mammalian
    Mutation
    embedded I-Ani I TS from 163-185bp, truncated 25 …
    Promoter
    SFFV
    Available since
    Nov. 4, 2013
    Availability
    Academic Institutions and Nonprofits only
    45577-plasmid-map-sequence-id-67848

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  8. pSB-CMV-MCS-Puro mCherry-Sec 61B -P2A-EGFP-LMNB1

    Plasmid
    #250911
    Purpose
    Dual color expression of mCherry tagged human Sec 61B and EGFP tagged zebrafish LaminB1 in mammalian cell lines
    Depositor
    Philipp Niethammer
    Publication
    Shen et al Nat Cell Biol. 2025 Dec 9. doi: 10.1038/
    Insert
    mCherry-Sec61B-P2A-EGFP-LMNB1 (SEC61B, lmnb1 Zebrafish, Human)
    Tags
    EGFP on LMNB1 and mCherry on Sec61b
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    March 4, 2026
    Availability
    Academic Institutions and Nonprofits only
    250911-plasmid-map-sequence-id-501821

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  12. pcDNA3.1-myc:Skp1(dC111):gs6:TEVs:gs6:fLuc:HA

    Plasmid
    #228530
    Purpose
    Firefly luciferase in fusion with a fragment of human Skp1 protein through a flexible linker and a TEV viral protease cleavage site. Expression of fLuc protein is deinhibited by TEV site cleavage.
    Depositor
    Roman Jerala
    Publication
    Verbic et al ACS Synth Biol. 2024 Mar 15;13(3):792-80
    Insert
    myc:Skp1(dC111):gs6:TEVs:gs6:fLuc:HA (SKP1 Photinus pyralis, Synthetic, Human)
    Tags
    HA and myc
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Dec. 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    228530-plasmid-map-sequence-id-456690

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  13. mEosEM-E_Lamin A

    Plasmid
    #226338
    Purpose
    Photoswitchable fluorescent protein for correlative light-electron microscopy; nuclear envelope-targeted mEosEM-E for mammalian expression
    Depositor
    Pingyong Xu
    Publication
    Peng et al Cells. 2022 Mar 23;11(7):1077. doi: 10.3
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Lamin A (LMNA Human)
    Tags
    mEosEM-E
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Dec. 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    226338-plasmid-map-sequence-id-456227

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  14. mCherry-CRY2–mPKCε-CAT-HA

    Plasmid
    #190483
    Purpose
    Expresses a fluorescently-tagged, optogenetically activated PKC-epsilon. Used in tandem with CIBN-CAAX
    Depositor
    Diomedes Logothetis
    Publication
    Gada et al J Biol Chem. 2022 May;298(5):101893. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mCherry-CRY2–mPKCε-CAT-HA (Prkce Mouse, Synthetic)
    Tags
    Fused to CRY2, HA, and mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Jan. 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    190483-plasmid-map-sequence-id-382847

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  15. pTRIP TRE BI_SEPT9_i1-14-GFP11_SEPT9_i1-14-GFP10

    Plasmid
    #180351
    Purpose
    mammalian co-expression of human SEPT9_i1 fused to GFP10 and of human SEPT9_i1 fused to GFP11
    Depositor
    Manos Mavrakis
    Publication
    Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301
    Inserts
    SEPTIN9_v1 (SEPTIN9 Human)
    SEPTIN9_v1 (SEPTIN9 Human)
    IRES-TagBFP
    Use
    Lentiviral
    Tags
    GFP10 and GFP11
    Expression
    Mammalian
    Promoter
    bidirectional TRE
    Available since
    Dec. 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    180351-plasmid-map-sequence-id-357912

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  18. pelB-MBP-P13-ss

    Plasmid
    #137065
    Purpose
    E. coli expression clone (T7lac promoter) for mature P13 (aa 22-179) from B. burgdorferi with N-terminal PelB signal peptide + His10 + mature maltose binding protein + TEV protease cleavage
    Depositor
    Debra Hansen
    Publication
    Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10
    Insert
    AAC66426.1 (BB_0034 Borrelia burgdorferi B31)
    Tags
    PelB signal peptide + His10 + maltose binding pro…
    Expression
    Bacterial
    Mutation
    mature P13: lacks the P13 signal peptide
    Promoter
    T7lac
    Available since
    April 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    137065-plasmid-map-sequence-id-291955

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Showing: 6981 - 7000 of 11809 results