We narrowed to 17,786 results for: Met;

1. PH-PLCD1_mScarlet-H_IRES_SYFP2_PH_N1

   Plasmid

   #85070

   Purpose

   Yellow/red FRET biosensor for monotoring plasmamembrane PtdIns(4,5)P2 levels (PIP2)

   Depositor

   Dorus Gadella

   Article

   Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

   Insert

   PH-PLCD1 (PLCD1 Human)
   

   Use

   Tags

   IRES-sYFP2-PH and mScarlet-H

   Expression

   Mammalian

   Mutation

   PLCD aa 1–170

   Promoter

   CMV

   Available since

   March 16, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. SNF-5 pXOOM

   Plasmid

   #48697

   Purpose

   Expressed SNF-5 in Xenopus oocytes or mammalian cells

   Depositor

   Dmitri Boudko

   Article

   Metzler et al J Exp Biol. 2013 Aug 1;216(Pt 15):2843-5

   Insert

   snf-5 (snf-5 Nematode)
   

   Use

   Xenopus oocyte expression

   Tags

   Expression

   Mammalian

   Mutation

   K42E (when compared to NP_496735.1)

   Promoter

   T7

   Available since

   Dec. 20, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBluescriptII[attB/Ins1]

   Plasmid

   #74100

   Purpose

   "intermediate vector" for the two-step cloning method (Wang and Szaro, Dev Biol, 2015)

   Depositor

   Ben Szaro

   Article

   Wang et al Dev Biol. 2015 Feb 1;398(1):11-23. doi:

   Inserts

   Phi C31 attb element
   Dual chicken beta-globin hypersensitive site 4 (HS4) insulators
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. peCALWY-2

   Plasmid

   #22234

   Depositor

   Maarten Merkx

   Article

   Vinkenborg et al Nat Methods. 2009 Oct;6(10):737-40. doi:

   Insert

   eCALWY-2 Zn(II) sensor (ATOX1 GFP's, Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   sequence identical to eCALWY-1, but linkerlength …

   Promoter

   Available since

   Nov. 20, 2009

   Availability

   Academic Institutions and Nonprofits only

5. TNF-SBP-mCherry_puromycin

   Plasmid

   #65285

   Purpose

   TNF reporter only (no hook) (RUSH system)

   Depositor

   Franck Perez

   Article

   Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

   Insert

   TNF (TNF Human)
   

   Use

   Tags

   Streptavidin Binding Protein (SBP) and mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCVL Traffic Light Reporter 1.1 (Ani target) Ef1a Puro

   Plasmid

   #31480

   Depositor

   Andrew Scharenberg

   Article

   Certo et al Nat Methods. 2011 Jul 10;8(8):671-6. doi

   Insert

   Traffic Light Reporter 1.1 (ani target)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   mCherry has M9S, M16L to reduce background mCherr…

   Promoter

   Available since

   Aug. 22, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET21-pelB-LaG-17

   Plasmid

   #172747

   Purpose

   Bacterial expression of HIS-tagged anti-GFP nanobody LaG-17; HIS tag preceded by HRV 3C/PreScission cleavage site.

   Depositor

   Michael Rout

   Article

   Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

   Insert

   LaG-17
   

   Use

   Tags

   6xHIS and pelB leader for periplasmic secretion

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-SMVP-Cas9N-U6-gRNA TdL

   Plasmid

   #80938

   Purpose

   Expresses Cas9N in mammalian cells; expresses gRNA TdL for Ai9 cleavage.

   Depositor

   George Church

   Article

   Chew et al Nat Methods. 2016 Sep 5. doi: 10.1038/nm

   Insert

   Cas9N
   

   Use

   AAV, CRISPR, and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRSFDuet-1_StefinA

   Plasmid

   #182402

   Purpose

   Bacterial expression of stefinA

   Depositor

   Dan Mulvihill

   Article

   Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

   Insert

   stefin (CSTA Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. cmv-sfGFP-GB1-scAB-ubc-nls-pcp-5xSunTag(SRv1-2P)

    Plasmid

    #185800

    Purpose

    one plasmid encoding scFv GFP and pcp-5xSunTag components for SunRISER SRv1

    Depositor

    Robin Lee

    Article

    Guo et al Cell Rep Methods. 2022 May 25;2(6):10022

    Insert

    cmv-sfGFP-GB1-scAB--ubc-nls-pcp-5xSunTag
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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