We narrowed to 9,182 results for: Pol

1. pCAG-lox-inv[Talpha1-HA-Dre]-lox-mOrange2-NEDD1-gTBD

   Plasmid

   #196894

   Purpose

   Neuron-specific expression of the gamma-tubulin-binding domain (gTBD) of NEDD1 fused to mOrange aimed to displace endogenous gamma-TuRC from the centrosome. Used in combination with Talpha1-iCre-pA plasmids

   Depositor

   Frank Bradke

   Article

   Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

   Insert

   N-gTBD-mOrange2 (NEDD1 Human)
   

   Use

   Cre/Lox; Dre/rox

   Expression

   Mammalian

   Promoter

   Quimeric CAG

   Available Since

   May 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAG-lox-inv[Talpha1-Dre-PA]-lox-2xHA-CAMSAP3

   Plasmid

   #196895

   Purpose

   Neuron-specific expression of Calmodulin Regulated Spectrin Associated Protein Family Member 3 (CAMSAP3) fused to 2xHA-tags. Used in combination with Talpha1-iCre-pA plasmids

   Depositor

   Frank Bradke

   Article

   Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

   Insert

   2xHA-CAMSAP3 (Camsap3 Mouse)
   

   Use

   Cre/Lox; Dre/rox

   Expression

   Mammalian

   Promoter

   Quimeric CAG

   Available Since

   May 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBactin-mOrange2-Cdk5rap2-CTD

   Plasmid

   #196855

   Purpose

   Expression of the centrosome targeting carboxy-terminal domain (CTD) of Cdk5rap2 fused to mOrange2. Used to displace endogenous Cdk5rap2 from centrosomes.

   Depositor

   Frank Bradke

   Article

   Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

   Insert

   mOrange2-Cdk5rap2-CTD (Cdk5rap2 Discosoma sp.)
   

   Expression

   Mammalian

   Mutation

   None (wt)

   Promoter

   Chicken bactin (plus Chicken bactin intron)

   Available Since

   May 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBactin-AcGFP-Cdk5rap2-CTDdeltaCBD

   Plasmid

   #196856

   Purpose

   Expression of the centrosome targeting carboxy-terminal domain (CTD) laking the centrosome binding domain (deltaCBD) of Cdk5rap2 fused to AcGFP. It cannot displace Cdk5rap2 from centrosomes

   Depositor

   Frank Bradke

   Article

   Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

   Insert

   AcGFP-Cdk5rap2-CTDdeltaCBD (Cdk5rap2 Rat)
   

   Expression

   Mammalian

   Mutation

   Lacking the centrosome-targeting domain (CTD)

   Promoter

   Chicken bactin (plus Chicken bactin intron)

   Available Since

   May 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pB80-hsKIF5B(1-560)Rigor-L-mVenus-L-SspB(micro)

   Plasmid

   #193721

   Purpose

   Optogenetic coupling to dimeric human kinesin-1 (KIF5B aa1-807) via SSPB(micro) for anterograde transport

   Depositor

   Lukas Kapitein

   Article

   Burute et al Sci Adv. 2022 Nov 4;8(44):eabo2343. doi:

   Insert

   hsKIF5B(1-560)Rigor-L-mVenus-L-SspB(micro) (KIF5B Synthetic, Human)
   

   Tags

   mVenus-SSPB(micro)

   Expression

   Mammalian

   Mutation

   hsKIF5B(aa1-560), RIGOR: G234A; mVenus: Met1Del, …

   Promoter

   Chicken beta-actin

   Available Since

   March 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV2-UbC-3xNLS-mScarlet-I

   Plasmid

   #191099

   Purpose

   To express a bright monomeric red FP to label eucaryotic cell nuclei. To be used in tissue clearing methods and other fluorescent microscopy methods

   Depositor

   Pantelis Tsoulfas, Murray Blackmore

   Article

   Wang et al Elife. 2022 Jul 15;11. pii: 76254. doi:

   Insert

   3xNLS-mScarlet-I
   

   Use

   AAV and Synthetic Biology

   Tags

   Three repeats of the nuclear localizzation seque…

   Expression

   Mammalian

   Mutation

   optimized to human codon usage

   Promoter

   UbC

   Available Since

   Dec. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. ATX3 13Q- I77K Q78K W87K

   Plasmid

   #185908

   Purpose

   Expresses ataxin-3 full length with 3 UIMs and 13Q in the Poly-Q track-I77K, Q78K,W87K (N-terminal ) fused with His-Tag and TEV cleavage sequence (C-terminal).

   Depositor

   Sandra Macedo Ribeiro

   Article

   Figueiredo et al Cells. 2022 Jun 19;11(12). pii: cells111

   Insert

   ataxin-3 full length with 3 UIMs and 13Q in the Poly-Q track and point mutations I77K, Q78K and W87K (ATXN3 Synthetic, Human)
   

   Tags

   6x His-Tag and TEV cleavage site

   Expression

   Bacterial

   Mutation

   Point mutations in Atx3 gene I77K Q78K and W87K

   Available Since

   Aug. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGEX2T-TEV CHMP1B 186-199 L188A/L192A

   Plasmid

   #108288

   Purpose

   Expresses residues 186-199 with L to A mutations at residues 188 and 192 of CHMP1B with an N-terminal GST tag and a TEV cleavage site; Internal ID: WISP 09-286

   Depositor

   Wesley Sundquist

   Article

   Nguyen et al Nat Struct Mol Biol. 2020 Apr;27(4):392-

   Insert

   CHMP1B (CHMP1B Human)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   changed L 188 to A, changed L 192 to A

   Available Since

   April 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGEX2T-TEV CHMP1B 186-199 L192A/L195A

   Plasmid

   #108290

   Purpose

   Expresses residues 186-199 with L to A mutations at residues 192 and 195 of CHMP1B with an N-terminal GST tag and a TEV cleavage site; Internal ID: WISP 09-287

   Depositor

   Wesley Sundquist

   Article

   Nguyen et al Nat Struct Mol Biol. 2020 Apr;27(4):392-

   Insert

   CHMP1B (CHMP1B Human)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   changed L 188 to A, changed L 192 to A

   Available Since

   April 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pKHH030_sgAMBRA1#2

    Plasmid

    #174147

    Purpose

    Lentiviral vector expressing a sgRNA against the human AMBRA1 gene

    Depositor

    Julien Sage

    Article

    Chaikovsky et al Nature. 2021 Apr;592(7856):794-798. doi:

    Insert

    AMBRA (AMBRA1 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    Sept. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pKHH030_sgAMBRA1#1

    Plasmid

    #174146

    Purpose

    Lentiviral vector expressing a sgRNA against the human AMBRA1 gene

    Depositor

    Julien Sage

    Article

    Chaikovsky et al Nature. 2021 Apr;592(7856):794-798. doi:

    Insert

    AMBRA (AMBRA1 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    Sept. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. CPH3569 (YCp LEU2 Rpb1 P1455_E1456InsGGGGGLEVLFQGP(H)10,K1487N,D1497K; msLink2)

    Plasmid

    #91820

    Purpose

    Yeast expression of S. cerevisiae Rpb1 with an internal PreScission protease site and 10xHis tag for purification of the linker-CTD

    Depositor

    Chris Hill

    Article

    Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1

    Insert

    RPO21 (RPO21 Budding Yeast)
    

    Tags

    10xHis

    Expression

    Yeast

    Mutation

    5xGly linker, PreScission Protease site (LEVLFQGP…

    Promoter

    Endogenous Rpb1 promoter

    Available Since

    March 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCJ200

    Plasmid

    #162673

    Purpose

    pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating S136C and a sequence Tyr75:Phe104 to introduce a 6th disulfide bond as in AoFaeB-2.

    Depositor

    Gregg Beckham

    Article

    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11

    Insert

    MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    S136C and a sequence Y75-F104 to introduce a 6th…

    Promoter

    T7/lac

    Available Since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCJ210

    Plasmid

    #162683

    Purpose

    pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating S136C, G489C, S530C, and a sequence Tyr75:Phe104 to introduce a 6th and 7th disulfide bond as in AoFaeB-2.

    Depositor

    Gregg Beckham

    Article

    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11

    Insert

    MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    S136C, G489C, S530C, and a sequence Y75-F104 to i…

    Promoter

    T7/lac

    Available Since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCJ220

    Plasmid

    #162686

    Purpose

    pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating lid deletion (Gly251:Thr472) and C224W and C529S mutations from PETase active site.

    Depositor

    Gregg Beckham

    Article

    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11

    Insert

    MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1) with lid deletion and C224W and C529S mutations
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    deltaG251-T472, C224W, C529S; codon optimized for…

    Promoter

    T7/lac

    Available Since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCJ221

    Plasmid

    #162687

    Purpose

    pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating lid deletion (Gly251:Thr472) and C224H and C529F mutations from PETase active site.

    Depositor

    Gregg Beckham

    Article

    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11

    Insert

    MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), with lid deletion and C224H and C529F mutations
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    deltaG251-T472, C224H, C529F; codon optimized for…

    Promoter

    T7/lac

    Available Since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1-N-Myc_DW164AA 1-205 USP7

    Plasmid

    #131254

    Purpose

    Mammalian expression of a mutant USP7 TRAF-like domain (DW164AA), with an N-terminal Myc tag

    Depositor

    Roger Woodgate

    Article

    Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

    Insert

    Ubiquitin carboxyl-terminal hydrolase 7 (USP7 Human)
    

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Expresses a mutant form of our pcDNA3.1-N-Myc_1-2…

    Available Since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1-N-Myc_DED/AAA 535-888 USP7

    Plasmid

    #131255

    Purpose

    Mammalian expression of USP7 UBL domains 1-3, with an N-terminal Myc tag and mutations in UBL2 (corresponding to DE758AA_D764A of the full-length protein)

    Depositor

    Roger Woodgate

    Article

    Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

    Insert

    Ubiquitin carboxyl-terminal hydrolase 7 (USP7 Human)
    

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    A mutant form of our pcDNA3.1-N-Myc_535-888 USP7 …

    Available Since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1-N-Myc_DW164AA + DED/AAA USP7

    Plasmid

    #131256

    Purpose

    Mammalian expression of N-terminally Myc-tagged USP7, with mutations in the TRAF-like domain (DW164AA) and UBL2 (DE758AA_D764A)

    Depositor

    Roger Woodgate

    Article

    Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

    Insert

    Ubiquitin carboxyl-terminal hydrolase 7 (USP7 Human)
    

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Contains point mutations that convert USP7 aspart…

    Available Since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGK-Ldb3-mKate2

    Plasmid

    #128766

    Purpose

    Fluorescent mKate reporter for Ldb3

    Depositor

    Deepak Srivastava

    Article

    Stone et al Cell Stem Cell. 2019 Jul 3;25(1):87-102.

    Insert

    Ldb3 promoter (Ldb3 Mouse)
    

    Expression

    Mammalian

    Promoter

    Ldb3

    Available Since

    Nov. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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