We narrowed to 17,821 results for: Por

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tdTomato-KIF1A

Plasmid

#102850

Purpose

Overexpresses KIF1A and cell fill TdTomato

Depositor

Erik Dent

Article

McVicker et al Nat Commun. 2016 Sep 23;7:12741. doi: 10

Insert

KIF1A (Kif1a Rat)

Tags

tdTomato

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 30, 2018

Availability

Academic Institutions and Nonprofits only

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pDN34

Plasmid

#61343

Purpose

mCherry fused to LINuS, a light-inducible nuclear localization signal (biNLS2/PKIt NES)

Depositor

Barbara Di Ventura, Roland Eils

Article

Niopek et al Nat Commun. 2014 Jul 14;5:4404. doi: 10.

Insert

mCherry

Tags

GS linker-AsLov2-biNLS2 and PKIt NES

Expression

Mammalian

Promoter

CMV

Available Since

May 13, 2015

Availability

Academic Institutions and Nonprofits only

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pSpCas9_T2A_mCherry sgBFP

Plasmid

#192929

Purpose

All-in-one plasmid for cutting BFP region in DSB Spectrum V2

Depositor

Michael Yaffe

Article

van de Kooij et al Nat Commun. 2022 Sep 8;13(1):5295. doi:

Insert

SpCas9

Tags

T2A mCherry

Expression

Mammalian

Available Since

Dec. 7, 2022

Availability

Academic Institutions and Nonprofits only

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EGFP-uTEV1-CB5

Plasmid

#171007

Purpose

HiLITR protease with CB5 C-terminal tmd targeting information (ER)

Depositor

Alice Ting

Article

Coukos et al Elife. 2021 Aug 20;10. pii: 69142. doi:

Insert

EGFP-uTEV1-CB5(tmd)

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Promoter

pTRE-tight (TetOn)

Available Since

Aug. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pC0068 PspCas13 (B12) His6-TwinStrep-SUMO-BsaI

Plasmid

#115219

Purpose

Expresses PspCas13b from a SUMO-tagged bacterial expression construct

Depositor

Feng Zhang

Article

Gootenberg et al Science. 2018 Apr 27;360(6387):439-444.

Insert

PspCas13

Tags

SUMO

Expression

Bacterial

Available Since

Sept. 21, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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EGFP-uTEV1-NES

Plasmid

#171006

Purpose

HiLITR protease with C-terminal NNES (Cytoplasmic)

Depositor

Alice Ting

Article

Coukos et al Elife. 2021 Aug 20;10. pii: 69142. doi:

Insert

EGFP-uTEV1-NES

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Promoter

pTRE-tight (TetOn)

Available Since

Aug. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-InPAkt

Plasmid

#181914

Purpose

Indicator of Phosphoinositides using Akt; genetically encoded FRET-based sensor for monitoring 3-phosphoinositide dynamics in living cells.

Depositor

Jin Zhang

Article

Ananthanarayanan et al Proc Natl Acad Sci U S A. 2005 Oct 18;10

Insert

InPAkt

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Promoter

CMV

Available Since

July 6, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-nuclear-EKAR4

Plasmid

#174439

Purpose

Nuclear-targeted EKAR4.

Depositor

Jin Zhang

Article

Keyes et al Elife. 2020 May 26;9. pii: 57410. doi: 1

Insert

nuclear-EKAR4

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pAS_4xMma PylT sfGPF 150TAG

Plasmid

#154766

Purpose

plasmid with 4xMma PylT cassette and amber suppression reporter sfGFP 150 TAG stop, for transient transfection or stable, piggybac-mediated, integration

Depositor

Simon Elsaesser

Article

Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021

Insert

sfGFP

Expression

Mammalian

Mutation

150TAG in GFP reporter, U25C in PylT

Promoter

EF1

Available Since

Sept. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pJJR82

Plasmid

#75027

Purpose

EGFP^SEC^3xFlag vector with ccdB markers for cloning homology arms

Depositor

Mike Boxem

Article

Ramalho et al Development. 2020 Jul 22;147(14):dev1880

Insert

EGFP^SEC^3xFlag

Use

CRISPR

Tags

3xFLAG and C. elegans codon optimized EGFP

Expression

Worm

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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DR5rev::erRFP

Plasmid

#61011

Purpose

Auxin signalling reporter (DR5rev) driving RFP (ER-targeted)

Depositor

Alexis Maizel

Article

Marin et al Plant Cell. 2010 Apr;22(4):1104-17. doi:

Insert

DR5rev::erRFP:35S_term

Expression

Plant

Promoter

DR5rev::erRFP:35Sterm

Available Since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pSCMV-H2B-FT-Medium

Plasmid

#157670

Purpose

Retroviral vector for expressing chimeric human histone H2B fused with the "Medium" variant of the monomeric color-changing fluorescent timer (FT) in mammalian cells.

Depositor

Shangqin Guo

Article

Eastman et al Cell Rep. 2020 Jun 23;31(12):107804. doi

Insert

Histone H2B

Use

Retroviral

Tags

Medium-FT

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pDisplay-GRAB_eCB2.0-IRES-mCherry-CAAX

Plasmid

#164611

Purpose

Express the endocannabinoid sensor GRAB_eCB2.0 in mammalian cells

Depositor

Yulong Li

Article

Dong et al Nat Biotechnol. 2021 Nov 11. pii: 10.103

Insert

Endocannabinoid sensor GRAB_eCB2.0

Expression

Mammalian

Mutation

S383T

Promoter

CMV

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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MelanoTag-PURO (pLJC5-GPR143-mScarlet-3xHA)

Plasmid

#160369

Purpose

Stable expression of GPR143-mScarlet-3xHA fusion protein for the isolation of melanosomes.

Depositor

David Sabatini

Article

Adelmann et al Nature. 2020 Nov 18. pii: 10.1038/s41586

Insert

GPR143 (GPR143 Human)

Use

Lentiviral

Tags

mScarlet-3xHA

Available Since

Nov. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pSCMV-H2B-FT-Fast

Plasmid

#157669

Purpose

Retroviral vector for expressing chimeric human histone H2B fused with the "Fast" variant of the monomeric color-changing fluorescent timer (FT) in mammalian cells.

Depositor

Shangqin Guo

Article

Eastman et al Cell Rep. 2020 Jun 23;31(12):107804. doi

Insert

Histone H2B

Use

Retroviral

Tags

Fast-FT

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pSCMV-H2B-FT-Slow

Plasmid

#157671

Purpose

Retroviral vector for expressing chimeric human histone H2B fused with the "Slow" variant of the monomeric color-changing fluorescent timer (FT) in mammalian cells.

Depositor

Shangqin Guo

Article

Eastman et al Cell Rep. 2020 Jun 23;31(12):107804. doi

Insert

Histone H2B

Use

Retroviral

Tags

Slow-FT

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-GRAB_OTmut

Plasmid

#185388

Purpose

Expresses the genetically-encoded fluorescent oxytocin(OT) control sensor GRAB_OTmut in neurons

Depositor

Yulong Li

Article

Qian et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Has Service

AAV1 and AAV9

Insert

GPCR activation based oxytocin control sensor GRAB_OTmut

Use

AAV

Promoter

hSyn

Available Since

Sept. 20, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-pmEKAR4

Plasmid

#174440

Purpose

Plasma membrane-targeted EKAR4.

Depositor

Jin Zhang

Article

Keyes et al Elife. 2020 May 26;9. pii: 57410. doi: 1

Insert

pmEKAR4

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT_CMV_NES-Caprola_05-mEGFP

Plasmid

#194683

Purpose

CMV driven expression of the calcium recorder Caprola_05 in mammalian cell lines fused to mEGFP

Depositor

Kai Johnsson

Article

Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

Insert

Caprola_05-mEGFP

Tags

mEGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pLYS5-MCU-Flag

Plasmid

#50054

Purpose

Expression of human MCU in mammalian cells

Depositor

Vamsi Mootha

Article

Sancak et al Science. 2013 Nov 14.

Insert

MCU-Flag (MCU Human)

Tags

Flag

Expression

Mammalian

Available Since

Jan. 21, 2014

Availability

Academic Institutions and Nonprofits only

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