We narrowed to 58,797 results for: Ran

1. pLCRISPR-CMV-cGAS3

   Plasmid

   #102610

   Purpose

   CRISPR/Cas9 lentivector targeting human cGAS

   Depositor

   Nicolas Manel

   Article

   Gentili et al Science. 2015 Sep 11;349(6253):1232-6. d

   Insert

   gRNA targeting human cGAS
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHR-CMV-TetO2_3C-Avi-His6_IRES-mRuby2

   Plasmid

   #113889

   Purpose

   Mammalian (inducible) protein expression, in vivo biotinylation, bicistronic expression of cytosolic mRuby2

   Depositor

   A. Radu Aricescu

   Article

   Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   3C-Avi-His6

   Promoter

   CMV-MIE-TetO2

   Available Since

   Aug. 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEYFP-hDF

   Plasmid

   #72584

   Purpose

   human dysferlin full length tagged with YFP

   Depositor

   Renzhi Han

   Article

   Xu et al PLoS One. 2011;6(11):e27884. doi: 10.137

   Insert

   dysferlin (DYSF Human)
   

   Tags

   EYFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. AAVS1-phiC31-LP-TC

   Plasmid

   #183755

   Purpose

   AAVS1 targeting construct with the phiC31 landing pad cassette.

   Depositor

   Richard Davis

   Article

   Blanch-Asensio et al Cell Rep Methods. 2022 Sep 22;2(10):1003

   Insert

   FRT-mCherry-attP-BleoR-F3
   

   Use

   Synthetic Biology

   Expression

   Mammalian

   Available Since

   Sept. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pNLF1W

   Plasmid

   #136404

   Purpose

   Expression of N-terminal NanoLuc luciferase fused proteins in mammalian cells. Genes can be cloned using Gateway cloning system. Cloned vectors can be used for NanoBRET assay.

   Depositor

   Sebastian Maurer

   Article

   Yang et al Nat Commun. 2018 Sep 14;9(1):3747. doi:

   Type

   Empty backbone

   Use

   Luciferase

   Tags

   NanoLuc luciferase

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. Sc++

   Plasmid

   #155011

   Purpose

   Expresses the codon-optimized, engineered Sc++ Cas9 nuclease in mammalian cells

   Depositor

   Joseph Jacobson

   Article

   Chatterjee et al Nat Biotechnol. 2020 May 11. pii: 10.103

   Insert

   Sc++
   

   Expression

   Mammalian

   Mutation

   T1227K, Loop Substitution from S. anginosus

   Promoter

   EF1ɑ

   Available Since

   Sept. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAC152-dual-dCas9VP64-sgExpression

   Plasmid

   #48238

   Purpose

   Dual expression construct expressing both dCas9VP64 and sgRNA from separate promoters

   Depositor

   Rudolf Jaenisch

   Article

   Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

   Insert

   dCas9
   

   Use

   CRISPR

   Tags

   HA Tag, HA-Tag, and VP64

   Expression

   Mammalian

   Mutation

   D10A;H840A

   Available Since

   Sept. 27, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLV-EF1a-mTagBFP2-2A-Puro_hU6-DJ-DR36-BsmBI

   Plasmid

   #226009

   Purpose

   DjCas13d guide RNA cloning backbone (lenti)

   Depositor

   Silvana Konermann

   Article

   Wei et al Cell Syst. 2023 Dec 20;14(12):1087-1102.

   Type

   Empty backbone

   Use

   Lentiviral

   Available Since

   Oct. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. W118-1_hTGM2-V5

   Plasmid

   #180413

   Purpose

   lentiviral transduction of human TGM2 transcript variant 2 (with a C-terminal V5 tag) gene

   Depositor

   Huda Zoghbi

   Article

   Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

   Insert

   TGM2 (TGM2 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAG/ATP8B4-HA

    Plasmid

    #209212

    Purpose

    Mammalian expression of ATP8B4

    Depositor

    Hye-Won Shin

    Article

    Takatsu et al J Biol Chem. 2011 Nov 4;286(44):38159-38

    Insert

    ATP8B4 (ATP8B4 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    Nov. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. AAV-p3-PE(1153-dRnH)-C-term

    Plasmid

    #187182

    Purpose

    p3-PE2-C-term(1153-dRnH) in AAV2 backbone (ITRs) targeting DNMT1

    Depositor

    Gerald Schwank

    Article

    Bock et al Sci Transl Med. 2022 Mar 16;14(636):eabl

    Insert

    PE2 from plasmid #132775 with shortened RnaseH domain
    

    Use

    AAV

    Available Since

    Aug. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. GST-Delta23C11orf83

    Plasmid

    #65849

    Purpose

    bacterial expression of GST (N-ter) - Delta 23 C11orf83/UQCC3 (UQCC3 protein depleted of its N-terminal transmembrane domain)

    Depositor

    Amos Bairoch

    Article

    Desmurs et al Mol Cell Biol. 2015 Apr;35(7):1139-56. d

    Insert

    UQCC3 depleted of its N terminal transmembrane part (UQCC3 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    deletion of the 23 first aa (TM)

    Available Since

    July 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCSIIbsr-Eevee-ROCK-NES

    Plasmid

    #209918

    Purpose

    A FRET biosensor for ROCK activity

    Depositor

    Michiyuki Matsuda

    Article

    Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi

    Insert

    Eevee-ROCK-NES
    

    Use

    Lentiviral

    Tags

    ECFP and YPet

    Available Since

    Nov. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCLJ-F2P-LoxP-PGK-NeoR-LoxP

    Plasmid

    #227503

    Purpose

    Inserts 500bp Prdm8 promoter in place of the Fgf5 promoter along with removable selection cassette

    Depositor

    Joanna Wysocka

    Article

    Jensen et al Mol Cell. 2025 Jan 16;85(2):347-361.e7.

    Insert

    Prdm8 promoter
    

    Use

    AAV and Cre/Lox

    Available Since

    May 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRSFDuet-gst-xr

    Plasmid

    #214761

    Purpose

    Xylose reductase from Hypocrea jecorina fused with GST-tagged and codon optimized for expression in E. coli

    Depositor

    Pimchai Chaiyen

    Article

    Jaroensuk et al J Biol Chem. 2023 Dec 28:105598. doi: 10

    Insert

    glutathione S-transferases fused with xylose reductase
    

    Tags

    Glutathione S-transferases

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. C1-mCitrine-FKBP-EZRabd(t567D)

    Plasmid

    #155227

    Purpose

    Acute increase in MPA when used with FRB-FKBP dimerizaton system upon addition with Rapamycin

    Depositor

    Tobias Meyer

    Article

    Bisaria et al Science. 2020 Jun 12;368(6496):1205-1210

    Insert

    mCitrine fused to FKBP and the actin BD of Ezrin (T567D), aa552-586 for use with FRB and rapamycin induced dimerization system
    

    Tags

    mCitrine

    Expression

    Mammalian

    Mutation

    Changed Thr 567 to Asp in original Ezrin

    Available Since

    Aug. 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSNR52-sgTEF1

    Plasmid

    #46922

    Purpose

    Yeast CEN/ARS vector (Ura3) that contains sgRNA controlled by SNR 52 promoter, targeting endogenous TEF1 promoter

    Depositor

    Stanley Qi, Jonathan Weissman

    Article

    Gilbert et al Cell. 2013 Jul 9. pii: S0092-8674(13)008

    Insert

    sgTEF1 promoter (TEF1 Budding Yeast)
    

    Use

    CRISPR

    Expression

    Yeast

    Promoter

    SNR52

    Available Since

    Oct. 1, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1-GFP-FUS-IDR

    Plasmid

    #183236

    Purpose

    Expression of eGFP fused to a intrinsically disordered regions of the FUS protein

    Depositor

    Jorge Ferrer

    Article

    Beucher et al Nat Cell Biol. 2022 Oct 6. pii: 10.1038/

    Insert

    EGFP
    

    Tags

    FLAG tag and Fusion to an IDR from the FUS protei…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHR-CMV-TetO2_3C-Twin-Strep_IRES-mTurquoise2

    Plasmid

    #113886

    Purpose

    Mammalian (inducible) protein expression, bicistronic expression of cytosolic mTurquoise2

    Depositor

    A. Radu Aricescu

    Article

    Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    3C-Twin-Strep

    Promoter

    CMV-MIE-TetO2

    Available Since

    Aug. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-CaMKII-WHaloCaMP1a

    Plasmid

    #205310

    Purpose

    Production of AAV particles encoding WHaloCaMP1a from the CaMKII promoter

    Depositor

    Eric Schreiter

    Article

    Farrants et al bioRxiv. 2023 Jul 19:2023.07.18.549527.

    Insert

    WHaloCaMP1a
    

    Use

    AAV

    Available Since

    Aug. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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