We narrowed to 8,578 results for: sgRNA

1. pU6-DHFR

   Plasmid

   #80329

   Purpose

   Allows for expression of an sgRNA in Toxoplasma gondii and confers resistance to pyrimethamine

   Depositor

   Sebastian Lourido

   Article

   Sidik et al Cell. 2016 Sep 8;166(6):1423-1435.e12. d

   Insert

   DHFR-TSc3
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   DHFR-TS 5' UTR

   Available since

   Sept. 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. AAVS1_Puro_hPGK1_mScarlet-I-TOSI_Donor

   Plasmid

   #178087

   Purpose

   HDR donor plasmid to introduce the mScarlet-I mTOR signaling indicator (TOSI) cassette to AAVS1 using AAVS1 T2 sgRNA.

   Depositor

   Yannick Doyon

   Article

   Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

   Insert

   mScarlet-I mTOR signaling indicator (TOSI)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Human PGK1

   Available since

   Dec. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSpCas9_gRNA1TERTKO_BsagRNA5tertko_2A-GFP

   Plasmid

   #198863

   Purpose

   Plasmid encoding for 2 gRNAs targeting the human TERT gene

   Depositor

   Agnel Sfeir

   Article

   Laprade et al Mol Cell. 2020 Jul 2;79(1):115-126.e6. d

   Insert

   TERT sgRNA (TERT Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   u6

   Available since

   April 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAC153-dual-dCas9VP96-sgExpression

   Plasmid

   #48239

   Purpose

   Dual expression construct expressing both dCas9VP96 and sgRNA from separate promoters

   Depositor

   Rudolf Jaenisch

   Article

   Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

   Insert

   dCas9
   

   Use

   CRISPR

   Tags

   HA-Tag, HA-tag, and VP96

   Expression

   Mammalian

   Mutation

   D10A H840A

   Promoter

   Available since

   Sept. 17, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBM019

   Plasmid

   #128179

   Purpose

   Construct for generating stable Cas9-expressing Toxoplasma gondii

   Depositor

   Sebastian Lourido

   Article

   Markus et al mSphere. 2019 Jun 26;4(3). pii: 4/3/e003

   Inserts

   sgRNA#1
   SpCas9
   Chloramphenicol acetyltransferase
   

   Use

   CRISPR; Toxoplasma gondii expression

   Tags

   3X FLAG and Nuclear Localization Signal

   Expression

   Mutation

   Promoter

   TUB1 (Toxoplasma gondii), U6 (Toxoplasma gondii),…

   Available since

   July 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDECKO-mCherry GFP

   Plasmid

   #78535

   Purpose

   Control plasmid (paired gRNAs against GFP)

   Depositor

   Roderic Guigo, Rory Johnson

   Article

   Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

   Insert

   gRNA1+scaffold+H1promoter+gRNA2
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 (for expressing sgRNA) and U6 promoter

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. LentiU6-hASCL1 gRNA_1-MS2-Puro

   Plasmid

   #192670

   Purpose

   Lentiviral expression of sgRNA targeting hASCL1 promoter to activate human ASCL1 transcription

   Depositor

   Jeffrey Miner

   Article

   Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

   Insert

   Human ASCL1 activating gRNA #1 (ASCL1 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. qTAG-C-Solo-mStayGold-PXN

   Plasmid

   #227316

   Purpose

   Donor template for mStayGold insertion into the C-terminus of the PXN locus. For focal adhesion visualization. To be co-transfected with sgRNA plasmid px330-PITCh-PXN (Addgene #227315)

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   PXN Homology Arms flanking a mStayGold Tag (PXN Human)
   

   Use

   CRISPR; Donor template

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Promoterless

   Available since

   Nov. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. Lenti-eCas9-sgMIB1

   Plasmid

   #140239

   Purpose

   Lentiviral vector expressing high-specificity eSpCas9(1.1) and a gRNA targeting human MIB1

   Depositor

   Urs Greber

   Article

   Bauer et al Cell Rep. 2019 Dec 17;29(12):3785-3795.e

   Insert

   sgRNA targeting human MIB1
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   May 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJY-RpABE-PDS3_IMS

    Plasmid

    #112875

    Purpose

    binary vector for IMS (Induced Mis-Splicing) of PDS3 in Arabidopsis

    Depositor

    Jae-Young Yun, Jin-Soo Kim

    Article

    Kang et al Nat Plants. 2018 Jun 4. pii: 10.1038/s41

    Inserts

    RPS5A promoter
    ABE7.10
    U6-sgRNA targeting PDS3
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Sept. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. qTAG-C-Solo-mScarlet-H2BC11

    Plasmid

    #207756

    Purpose

    Donor template for mScarlet insertion into the C-terminus of the H2BC11 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 Addgene #207755

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    H2BC11 Homology Arms flanking a mScarlet Tag (H2BC11 Human)
    

    Use

    CRISPR; Donor template

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 16, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. B270 + SPRTN sgSTOP

    Plasmid

    #100718

    Purpose

    B270 plasmid expressing SPRTN sgSTOP (cloned in BbsI site) in combination with ATP1A1 sgSTOP

    Depositor

    Alberto Ciccia

    Article

    Billon et al Mol Cell. 2017 Sep 4. pii: S1097-2765(17

    Insert

    sgSTOP targeting SPRTN (cloned using BbsI)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 promoters

    Available since

    Sept. 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pPV-H1-ccdB-mEF1α-RiH

    Plasmid

    #100598

    Purpose

    piggyBac vector for sgRNA cloning

    Depositor

    Akitsu Hotta

    Article

    Ishida et al Sci Rep. 2018 Jan 10;8(1):310. doi: 10.1

    Type

    Empty backbone

    Use

    Piggybac vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human H1 PolIII promoter, human FTH1 promoter, mo…

    Available since

    April 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pX330-EN1082_Rad21_STOP

    Plasmid

    #156450

    Purpose

    For transient expression of Cas9 and sgRNA targeting mouse RAD21 stop codon

    Depositor

    Elphege Nora

    Article

    Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

    Insert

    Cas9, RAD21 (Rad21 Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    none

    Promoter

    Available since

    Sept. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. c3GIC9n

    Plasmid

    #62192

    Purpose

    col1a1 targeting vector for inducible [TRE3G]-GFP-IRES-Cas9n (D10A variant) expression. Contains NsiI cloning site for U6-sgRNA cassettes

    Depositor

    Lukas Dow

    Article

    Dow et al Nat Biotechnol. 2015 Apr;33(4):390-4. do

    Insert

    humanized S. Pyogenes Cas9 (D10A)
    

    Use

    CRISPR

    Tags

    HA

    Expression

    Mutation

    D10A substitution (Cas9n)

    Promoter

    TRE3G

    Available since

    Aug. 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. tdTomato/pTREX-b

    Plasmid

    #68709

    Purpose

    Expresses tdTomato in pTREX-b vector which confers resistance to blasticidin. This vector is used for cloning a specific sgRNA by BamHI, to transfect Cas9-expressing Trypanosoma cruzi epimastigotes.

    Depositor

    Roberto Docampo

    Article

    Lander et al MBio. 2015 Jul 21;6(4). pii: e01012-15.

    Insert

    tdTomato
    

    Use

    Expression of tdtomato red fluorescence protein i…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pJG85

    Plasmid

    #89281

    Purpose

    Entry vector containing the TaU6 promoter and an Esp3I Golden Gate cloning site for cloning of the sgRNA, all between attL5 and attL2 sites

    Depositor

    Daniel Voytas

    Article

    Gil-Humanes et al Plant J. 2016 Dec 10. doi: 10.1111/tpj.1

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    May 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGL3-U6-pegRNA_SRD5A3-EGFP

    Plasmid

    #159781

    Purpose

    S. pyogenes pegRNA for C to A mutation at the SRD5A3 site of human cells using prime editing

    Depositor

    Xiaolong Wang

    Article

    Liu et al Cell Discov. 2020 Apr 28;6:27. doi: 10.1

    Insert

    spacer of pegRNA targeting SRD5A3 gene (SRD5A3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBTK615

    Plasmid

    #110609

    Purpose

    BTK assembled plasmid - used in Stage 2 assembly to target Cas9 or dCas9

    Depositor

    Jeffrey Barrick

    Article

    Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

    Insert

    sgRNA
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. FUG-T2A-Cas9

    Plasmid

    #75346

    Purpose

    Ubiquitin promoter expresses GFP and humanized spCas9 (from PX330) via a T2A motif. For cell transfection or use in lentiviral packaging. Use Pac1 and/or BstB1 sites to insert sgRNAs from PXL.

    Depositor

    Bryan Luikart

    Article

    Williams et al Sci Rep. 2016 May 10;6:25611. doi: 10.10

    Insert

    Cas9
    

    Use

    CRISPR and Lentiviral

    Tags

    GFP (via t2a)

    Expression

    Mammalian

    Mutation

    Promoter

    Ubiquitin

    Available since

    May 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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