We narrowed to 164,826 results for: Gene

1. pWJ68

   Plasmid

   #46571

   Purpose

   Same as pdCas9, but with the w-dCas9 fusion (w is fused at the N-terminal end of dCas9)

   Depositor

   Luciano Marraffini

   Article

   Bikard et al Nucleic Acids Res. 2013 Jun 12.

   Inserts

   tracrRNA
   w-dcas9
   CRISPR array
   

   Use

   CRISPR

   Tags

   rpoZ

   Expression

   Bacterial

   Mutation

   D10A, H840A

   Promoter

   Available Since

   Aug. 27, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pKB01_tagRFP

   Plasmid

   #69103

   Purpose

   Expresses tagRFP (codon adapted for Streptococcus pneumoniae); under control of Zinc inducible promoter PZn. Plasmid integrates in S. pneumoniae bgaA locus by double cross-over.

   Depositor

   Jan-Willem Veening

   Article

   Beilharz et al Appl Environ Microbiol. 2015 Aug 7. pii:

   Insert

   tagRFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Oct. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGL3-IE1

   Plasmid

   #52894

   Purpose

   baculovirus IE-1 promoter driving firefly luciferase

   Depositor

   Zach Adelman

   Article

   Anderson et al Insect Mol Biol. 2010 Aug;19(4):441-9. d

   Insert

   IE1 promoter
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   Promoter

   baculovirus IE1

   Available Since

   May 27, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pH6HTC_IGF2BP3-Halo

   Plasmid

   #175310

   Purpose

   Production of HaloTag fusion protein

   Depositor

   Claudia Kutter

   Article

   Atanasoai et al bioRxiv 2021.11.08.467743

   Insert

   IGF2BP3 (IGF2BP3 Human)
   

   Use

   Cell free expression

   Tags

   HaloTag-6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Oct. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. M4786 KanMX::hisG-URA3-HisG Disruptor Converter

   Plasmid

   #51690

   Purpose

   Yeast marker swap plasmid for converting KanMX3 to hisG-URA3-hisG

   Depositor

   David Stillman

   Article

   Voth et al Yeast. 2003 Aug;20(11):985-93.

   Insert

   hisG-URA3-hisG (URA3 Budding Yeast)
   

   Use

   Yeast marker swap

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   June 6, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCsB

   Plasmid

   #136068

   Purpose

   Acceptor plasmid for even level position 2, lacZ blue-white screening

   Depositor

   Jim Haseloff

   Article

   Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

   Insert

   lacZ
   

   Use

   Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   Nov. 30, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pCsC

   Plasmid

   #136069

   Purpose

   Acceptor plasmid for even level position 3, lacZ blue-white screening

   Depositor

   Jim Haseloff

   Article

   Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

   Insert

   lacZ
   

   Use

   Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   Nov. 30, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pCsE

   Plasmid

   #136070

   Purpose

   Acceptor plasmid for even level position 4, lacZ blue-white screening

   Depositor

   Jim Haseloff

   Article

   Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

   Insert

   lacZ
   

   Use

   Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   Nov. 30, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pCS2-TagRFPT.zf1

   Plasmid

   #61390

   Purpose

   mRNA synthesis of TagRFPT codon modified for zebrafish

   Depositor

   Harold Burgess

   Article

   Horstick et al Nucleic Acids Res. 2015 Jan 27. pii: gkv

   Insert

   TagRFPT.zf1
   

   Use

   Zebrafish expression

   Tags

   Expression

   Mutation

   Promoter

   sp6

   Available Since

   Jan. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pPIDCB2

    Plasmid

    #169900

    Purpose

    Empty dox inducible vector with piggyBac, insulators, and a DTS. The constitutive mClover3 is fused to H2B to allow for visualization of the nucleus.

    Depositor

    Richard Schneider

    Article

    Chu et al Biol Open. 2020 Oct 6;9(10). pii: bio.05

    Type

    Empty backbone

    Use

    Avian expression

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Dox inducible

    Available Since

    Dec. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pENTR R4 ATCC LacZ GCTT

    Plasmid

    #173668

    Purpose

    Destination vector for Golden Gate Cloning assemblies, blue-white screening

    Depositor

    Eric Marois

    Article

    Green et al Elife. 2023 Dec 5;12:e93142. doi: 10.755

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial and Insect

    Mutation

    Promoter

    Available Since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSBbi-TIR1-tTA/Pur

    Plasmid

    #171683

    Purpose

    constitutive expression of TIR1-myc and tTA in sleeping beauty transposon vector with selection marker (puromycin)

    Depositor

    Randy Poon

    Article

    Yeung et al Mol Biol Cell. 2021 May 12:mbcE21020051.

    Inserts

    tetracycline-controlled transactivator
    Puromycin
    EF-1a promoter
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a promoter and RPBSA promoter

    Available Since

    Oct. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. CMV-AsCas12f-WT

    Plasmid

    #204635

    Purpose

    Expression of wild-type AsCas12f in mammalian cells

    Depositor

    Weixin Tang

    Article

    Wu et al Nat Chem Biol. 2023 Jul 3. doi: 10.1038/

    Insert

    AsCas12f
    

    Use

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. TTC3_pCSdest

    Plasmid

    #53851

    Depositor

    Roger Reeves

    Article

    Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

    Insert

    TTC3 (TTC3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SP6 and CMV

    Available Since

    March 27, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pET47b-T7-AsCas12f-WT

    Plasmid

    #204640

    Purpose

    Expression of wild-type AsCas12f in E. coli

    Depositor

    Weixin Tang

    Article

    Wu et al Nat Chem Biol. 2023 Jul 3. doi: 10.1038/

    Insert

    AsCas12f
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Sept. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSuper-gmiR-122

    Plasmid

    #171554

    Purpose

    a human miR-122-expressing plasmid

    Depositor

    Yan Zeng

    Article

    Li et al Cells. 2019 Jul 30;8(8). pii: cells80807

    Insert

    miR-122 (MIR122 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-G12-mTGFB1

    Plasmid

    #219553

    Purpose

    mTGFB1 expression in mammlian cells. pAAV vector. Reporter plasmid.

    Depositor

    Vladislav Verkhusha

    Article

    Baloban et al Protein Sci. 2024 May;33(5):e4993. doi:

    Insert

    mTGFB1 (Tgfb1 Mouse)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    12xUAS with TATA-box minimal promoter

    Available Since

    May 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMH5

    Plasmid

    #52531

    Purpose

    expresses FLP recombinase in Drosophila

    Depositor

    Klaus Foerstemann

    Article

    Bottcher et al Nucleic Acids Res. 2014 Apr 19.

    Insert

    FLP recombinase (flp Budding Yeast)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    ubiquitin

    Available Since

    April 23, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. Aurli_150841_GZG

    Plasmid

    #162563

    Purpose

    Inactivation of Aurli_150841 (crtIBY) in Aurantiochytrium limacinum MYA-1381 with zeocin resistance driven by native GAPDH promoter/termiator system.

    Depositor

    Jackie Collier

    Article

    Rius et al Genome Biol Evol. 2023 Mar 3;15(3):evad0

    Insert

    shble
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    GAPDH

    Available Since

    May 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. UAS-Cre-T2A-miRFP670

    Plasmid

    #193581

    Purpose

    Fluorescent reporter for Gal4-induced Cre recombination

    Depositor

    Satoru Takahashi

    Article

    Yoshihara et al Exp Anim. 2022 Aug 5;71(3):385-390. doi:

    Insert

    miRFP670
    

    Use

    Cre/Lox

    Tags

    T2A

    Expression

    Mutation

    Promoter

    5X UAS

    Available Since

    Jan. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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